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Registros recuperados: 107
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A first generation genetic linkage map of the European flat oyster Ostrea edulis (L.) based on AFLP and microsatellite markers ArchiMer
Lallias, Delphine; Beaumont, A.r.; Haley, C.s.; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
This study presents the first genetic linkage map for the European flat oyster Ostrea edulis. AFLP and 20 microsatellite markers were genotyped in a three-generation pedigree comprised of 2 grand-parents, 2 parents and 92 progeny. Chi-square goodness of fit tests revealed high segregation distortion, which was significant for 32.8% of markers. Sixteen microsatellites and 235 AFLPs (170 AFLPs type 1:1 and 65 AFLPs type 3:1) were used to build sex-specific linkage maps using CriMap software. The first parental map (P1) consisted of 104 markers grouped in nine linkage groups, spanning 471.2 cM with an average spacing of 4.86 cM. The second parental map (P2) consisted of 117 markers grouped in ten linkage groups (which equals the haploid chromosome number),...
Tipo: Text Palavras-chave: Microsatellite; AFLP; Ostrea edulis; Flat oyster; Genetic linkage map.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2642.pdf
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A first genetic linkage map of the blue mussel Mytilus edulis ArchiMer
Lallias, Delphine; Hecquet, Céline; Boudry, Pierre; Lapegue, Sylvie; Beaumont, Andy.
The blue mussel, Mytilus edulis, is widely distributed in the northern hemisphere, from the White Sea to the Atlantic coast of southern France, and is an economically important species (1 million tons per year, FAO, 2002). At present however, little domestication has been achieved with bivalves, in contrast to agricultural crops, livestock and horticultural species like tomatoes, where selection and production is entirely controlled by man. For bivalves, despite their economic importance, no lines with selected characteristics have been taken to the production scale. There is therefore a need to make up for lost time. Our interest in genetic mapping sprang from some results showing a genetic component to some economically important traits like growth and...
Tipo: Text Palavras-chave: QTLs; AFLP; Genetic; Mussel; Mytilus edulis; Genetic linkage map.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3465.pdf
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A high density genetic map of maritime pine based on AFLPs Inra
Chagné, D.; Lalanne, C.; Madur, D.; Kumar, S.; Frigério, J.M.; Krier, C.; Decroocq, S.; Savouré, A.; Bou-Dagher-Kharrat, M.; Bertocchi, E.; Brach, J.; Plomion, C..
Tipo: Conference Paper Palavras-chave: AFLP; DOUBLE PSEUDO-TESTCROSS; TAILLE PHYSIQUE; CARTE GENETIQUE.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0300011664096195&uri=/notices/prodinra1/2009/12/
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A multivariate approach to explore the genetic variability in the F2 segregating population of a tomato second cycle hybrid JBAG
Cabodevila,V.G; Picardi,L.A; Pratta,G.R.
Segregating progeny from the tomato Second Cycle Hybrids (SCH) that were obtained from crossing RIL (Recombinant Inbred Lines) allows the detection of new genetic combinations that could increase genetic variability in F2 populations. The objectives of the present study were to evaluate eleven tomato quality traits in a segregating F2 population obtained from a SCH and, then, to characterize the molecular diversity by six AFLP (Amplified Fragment Length Polymorphism) primer combinations. Different multivariate analyses were used to assess the degree of concordance among these two approaches to detect genetic variability. Sixty-nine F2 plants were obtained by selfing the SCH (ToUNR18xToUNR1). The parental RIL were derived from an interspecific cross between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant breeding; Plant genetic resources; Fruit quality; AFLP; Multivariate analysis.
Ano: 2017 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332017000100001
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AFLP and microsatellites genetic divergence analyzes among 103 mango accessions. Repositório Alice
SANTOS, C. A. F.; LIMA NETO, F. P..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Mangifera indica; Divergência genética; AFLP; Manga; Melhoramento genético; Mango.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/961908
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AFLP marker analysis revealing genetic structure of the tree Parapiptadenia rigida (Benth.) Brenan (Leguminosae-Mimosoideae) in the southern Brazilian Tropical Rainforest Genet. Mol. Biol.
Souza,Laís Bérgamo de; Ruas,Eduardo A.; Rodrigues,Luana A.; Ruas,Claudete F.; Ruas,Paulo M..
Parapiptadenia rigida is a tropical early secondary succession tree characteristic of the Tropical Atlantic Rainforest. This species is of great ecological importance in the recovery of degraded areas. In this study we investigated the variability and population genetic structure of eight populations of P. rigida. Five AFLP primer combinations were used in a sample of 159 individuals representing these eight populations, rendering a total of 126 polymorphic fragments. The averages of percentage of polymorphic loci, gene diversity, and Shannon index were 60.45%, 0.217, and 0.322, respectively. A significant correlation between the population genetic variability and the population sizes was observed. The genetic variability within populations (72.20%) was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tropical tree; Genetic diversity; Population genetics; Conservation; AFLP.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400012
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AFLP marker linked to water-stress-tolerant bulks in barley (Hordeum vulgare L.) Genet. Mol. Biol.
Altinkut,A.; Kazan,K.; Gozukirmizi,N..
The amplified fragment length polymorphism (AFLP) assay is an efficient method for the identification of molecular markers, useful in the improvement of numerous crop species. Bulked Segregant Analysis (BSA) was used to identify AFLP markers associated with water-stress tolerance in barley, as this would permit rapid selection of water-stress tolerant genotypes in breeding programs. AFLP markers linked to water-stress tolerance was identified in two DNA pools (tolerant and sensitive), which were established using selected F2 individuals resulting from a cross between water-stress-tolerant and sensitive barley parental genotypes, based on their paraquat (PQ) tolerance, leaf size, and relative water content (RWC). All these three traits were previously shown...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bulked segregant analysis; Drought; Hordeum vulgare; AFLP; Paraquat.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100013
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AFLP markers and DNA barcodes indicate diverse cryptic species lineages withn Urobenus brasiliensis (Clitellata: Rhinodrilidae). Repositório Alice
LOCATELLI, M.; JAMES, S. W.; BROWN, G. G.; BARETTA, D.; BARTZ, M. L. C.; FORBES, A..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Minhoca; Espécie; Urobenus brasiliensis; AFLP; DNA.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/960846
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AFLP polymorphism in restored provenances of Ceiba aesculifolia within an urban heat island Phyton
Olvera-Mendoza,EI; Lara-Cabrera,SI; Sáenz-Romero,C; Lindig-Cisneros,R.
To quantify the effect on genetic diversity of restoring tree populations in an area under the influence of an urban heat island, we evaluated the genetic diversity of 72 Ceiba aesculifolia individuals, from a restoration experiment established from 2170 to 2260 m a.s.l. Reintroduced individuals were compared with the provenance from which the seeds were obtained, and two external provenances, for a total of 123 individuals. Samples were analyzed with three AFLP primer combinations. Polymorphisms of 38.4 to 62.5% were obtained. Genetic diversity estimated with the Simpson index ranged from 0.14 to 0.2. The provenances in the restoration site had higher diversity than the provenance that originated the seeds. Groups formed with provenances with similar...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; AFLP; Restoration ecology; Ceiba aesculifolia; Protected Natural Area.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200001
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AFLP-based genetic linkage maps of the blue mussel (Mytilus edulis) ArchiMer
Lallias, Delphine; Lapegue, Sylvie; Hecquet, Celine; Boudry, Pierre; Beaumont, A.
We report the construction of the first genetic linkage map in the blue mussel, Mytilus edulis. AFLP markers were used in 86 full-sib progeny from a controlled pair mating, applying a double pseudo-test cross strategy. Thirty-six primer pairs generated 2354 peaks, of which 791 (33.6%) were polymorphic in the mapping family. Among those, 341 segregated through the female parent, 296 through the male parent (type 1:1) and 154 through both parents (type 3:1). Chi-square goodness-of-fit tests revealed that 71% and 73% of type 1:1 and 3:1 markers respectively segregated according to Mendelian inheritance. Sex-specific linkage maps were built with mapmaker 3.0 software. The female framework map consisted of 121 markers ordered into 14 linkage groups, spanning...
Tipo: Text Palavras-chave: Mytilus edulis; Genetic linkage map; Blue mussel; AFLP.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2830.pdf
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Agronomic and molecular analyses for the characterisation of accessions in Tunisian olive germplasm collections Electron. J. Biotechnol.
Taamalli,Wael; Geuna,Filippo; Banfi,Riccardo; Bassi,Daniele; Daoud,Douja; Zarrouk,Mokhtar.
In this study, a total of 14 agronomic traits, five AFLP primer combinations and ten SSR loci were used to describe and to classify a group of Tunisian olive genotypes into groups based on molecular profiles and agronomic traits. The analysis of variance of the agronomical data revealed significant differences among accessions for all measured traits. The mean phenotypic dissimilarity (0.34 with a range of 0.08-0.6) was low in comparison to dissimilarity calculated using AFLP (0.50 with a range of 0.16-0.70) and SSR markers (0.76 with a range 0.35-0.94). The correlation between the agronomical dissimilarity matrix and the matrices of genetic dissimilarity based on SSR and AFLP markers was very weak: 0.156 (p = 0.05) and 0.185 (p = 0.05), respectively. The...
Tipo: Journal article Palavras-chave: Agronomic traits; AFLP; Olea europaea; SSR.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500002
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Agrupamiento de genotipos de nopal (Opuntia spp.) de México por medio de la técnica de AFLPs y características del fruto Phyton
Espinoza Sánchez,EA; Silos Espino,H; Flores Benitez,S; Valera Montero,LL; Rodríguez Salazar,E; Gallegos Vázquez,C; Guevara Lara,F; González Chavira,M; Guzmán Maldonado,HS.
Para contribuir al conocimiento de la diversidad genética del nopal se analizaron 85 genotipos representativos de Opuntia (silvestres y cultivados) por medio de la técnica de AFLPs y características del fruto. El análisis de AFLPs con los iniciadores Mse ICAC/Eco RI-AAG generaron más fragmentos polimórficos (57,5 en promedio) por genotipo; se obtuvo un dendrograma que no mostró una clara relación de acuerdo a la taxonomía conocida. En complemento al perfil genético, y para permitir una referencia morfológica o química directa, agrupamos a los genotipos de acuerdo al contenido de °Brix de sus frutos, debido a que esta es una característica primordial para su identificación visual y aprovechamiento de consumo directo. Se encontró que los genotipos que...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caracterización Molecular; Opuntia; AFLP; Dendrograma; Morfología del fruto.
Ano: 2014 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572014000200010
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An AFLP estimation of the outcrossing rate of Spondias tuberosa (Anacardiaceae), an endemic species to the Brazilian Semiarid region. Repositório Alice
SANTOS, C. A. F.; GAMA, R. N. C. de S..
The umbu tree (Spondias tuberosa) is one of the most important endemic species to the Brazilian tropical semiarid region. The umbu tree has edible fruits with a peculiar flavor that are consumed in natura or in a semi-industrialized form, such as jams, candies and juices. The majority of endemic species to Brazilian semiarid region have not been studied or sampled to form germplasm collections, which increases the risk of losing genetic variability of the adapted species to xerophytic conditions. The aim of this study was to estimate outcrossing rates in S. tuberosa using a multilocus mixed model in order to guide genetic resources and breeding programs of this species. DNA samples were extracted from 92 progenies of umbu trees, which were distributed...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Spondia tuberosa; AFLP; Sistema reprodutivo; Variabilidade genética; Planta nativa; Caatinga; Umbu; Melhoramento genético; Genetic variability.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/957559
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Análise da diversidade genética por aflp e identificação de marcadores associados à resistência a doenças em videira. Repositório Alice
OLIVEIRA, P. R. D. de; SCOTTON, D. C.; NISHIMURA, D. S.; FIGUEIRA, A..
RESUMO - Com objetivo de estudar diversidade genética e identificar marcadores associados à resistência ao míldio (Plasmopara viticola) e ao oídio (Uncinula necator), foram analisadas as cultivares de videira A 1976, CG 87746, CNPUV 154-27, Crimson Seedless, Gota de Ouro, Itália, Seyve Villard 12327 e Seyve Villard 12375. As análises de polimorfismo de comprimento de fragmento amplificado (AFLP) seguiram as etapas de digestão, ligação, pré-amplificação e amplificação. Efetuou-se a separação dos fragmentos amplificados em gel de 7% de poliacrilamida desnaturante (uréia 7M) e coloração com nitrato de prata. A similaridade foi estimada com base no coeficiente de Jaccard, e a agregação, por UPGMA. Foi gerada uma matriz de similaridade com bom ajustamento da...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: AFLP; Doença de Planta; Marcador Molecular; Míldio; Oídio; Resistência; Uva; Viticultura.
Ano: 2005 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/540917
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Análise da estrutura e diversidade molecular de populações de Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) associadas às culturas de milho e arroz no Rio Grande do Sul Neotropical Entomology
Busato,Gustavo R.; Grützmacher,Anderson D.; Oliveira,Antônio C. de; Vieira,Eduardo A.; Zimmer,Paulo D.; Kopp,Maurício M.; Bandeira,Juliana de M.; Magalhães,Taís R..
O objetivo do trabalho foi analisar a estrutura e a diversidade molecular de quatro populações de Spodoptera frugiperda (J.E. Smith) associadas às culturas de milho e arroz no Rio Grande do Sul. Foram coletadas lagartas de quatro populações em áreas isoladas (distanciadas em mais de 300 km) nos municípios de Santa Rosa (milho) e Uruguaiana (arroz irrigado) e em áreas adjacentes, no município de Pelotas (milho e arroz irrigado). A análise de 40 lagartas (10 de cada população), com cinco combinações de oligonucleotídios iniciadores geraram o total de 241 locos, dentre os quais 229 (95%) foram polimórficos. O dendrograma gerado pelo método de agrupamento UPGMA, baseado na matriz de similaridade de Dice, mostrou sobreposições entre as lagartas das populações...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Lagarta-do-cartucho; Lagarta-da-folha; AFLP; Biótipo.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000600008
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Análise morfoanatômica, bioquímica e molecular de dendezeiros (Elaeis guineensis Jacq.) regenerados por embriogênese somática em sistema de imersão temporária. Repositório Alice
GOMES, H. T..
O objetivo do trabalho foi avaliar a utilização de sistemas de imersão temporária na embriogênese somática de dendezeiros (Elaeis guineensis Jacq.), a partir de folhas imaturas de plantas adultas, caracterizar morfoanatômica e bioquimicamente os estádios embriogênicos obtidos no processo, além analisar os clones regenerados por marcadores ISSR e AFLP (MSAP). Num primeiro experimento, calos primários da variedade C2328 foram multiplicados por cinco subcultivos de 30 dias, em quatro diferentes sistemas de cultivo: semi-sólido, líquido sob agitação, além dos biorreatores de imersão temporária R.I.T.A.® e tipo frascos gêmeos (BIT). Em seguida, o biorreator mais eficiente foi avaliado isoladamente na multiplicação de calos de outras três variedades: B35-2932,...
Tipo: Tese/dissertação (ALICE) Palavras-chave: Arecaceae; Biorreatores de imersão temporária; Embrião somático; Variação somaclonal; ISSR; AFLP; MSAP; Morfogênese; Ontogênese; Micropropagação; Marcador molecular; Histologia; Fisiologia.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1065516
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Análisis preliminar de la variabilidad genética de Escallonia alpina y E. rubra (Escalloniaceae) Darwiniana
Morello,Santiago; Giussani,Liliana M; Sede,Silvana M.
Escallonia alpina y E. rubra (Escalloniaceae) comparten su área de distribución en el sur de los Andes y se diferencian por pocos caracteres morfológicos diagnósticos, tales como la disposición de las flores y la presencia y tipo de glándulas; sin embargo, en algunas poblaciones no es posible asignar los individuos a una u otra especie. Además, se han reconocido y descrito como híbridos varias formas de transición entre ambas. El objetivo de este trabajo es examinar la variabilidad genética poblacional de Escallonia alpina y E. rubra, y analizar si se corresponde con la variabilidad morfológica observada. Se estudió la morfología en 39 individuos de cinco poblaciones sobre los que también se realizó un análisis de AFLP. Se investigó la relación entre los...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AFLP; Escallonia; Patagonia; Sudamérica austral.
Ano: 2013 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0011-67932013000200004
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Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker Electron. J. Biotechnol.
Tiwari,Jagesh Kumar; Saurabh,Shaumaya; Chandel,Poonam; Singh,Bir Pal; Bhardwaj,Vinay.
Background: Genetic and epigenetic changes (DNA methylation) were examined in the tissue-culture propagated interspecific potato somatic hybrids between dihaploid Solanum tuberosum and S. pinnatisectum. Amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP) were applied to detect the genetic and epigenetic changes, respectively in the somatic hybrids mother plants (1st cycle) and their regenerants (30th cycles sub-cultured). Results: To detect genetic changes, eight AFLP primer combinations yielded a total of 329 scorable bands of which 49 bands were polymorphic in both mother plants and regenerants. None of the scorable bands were observed in term of loss of original band of mother plant or gain of novel band...
Tipo: Journal article Palavras-chave: AFLP; DNA methylation; In vitro propagation; MSAP; Potato; Somatic hybrids.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600005
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Analysis of genetic population structure in Acacia caven (Leguminosae, Mimosoideae), comparing one exploratory and two Bayesian-model-based methods Genet. Mol. Biol.
Pometti,Carolina L.; Bessega,Cecilia F.; Saidman,Beatriz O.; Vilardi,Juan C..
Bayesian clustering as implemented in STRUCTURE or GENELAND software is widely used to form genetic groups of populations or individuals. On the other hand, in order to satisfy the need for less computer-intensive approaches, multivariate analyses are specifically devoted to extracting information from large datasets. In this paper, we report the use of a dataset of AFLP markers belonging to 15 sampling sites of Acacia caven for studying the genetic structure and comparing the consistency of three methods: STRUCTURE, GENELAND and DAPC. Of these methods, DAPC was the fastest one and showed accuracy in inferring the K number of populations (K = 12 using the find.clusters option and K = 15 with a priori information of populations). GENELAND in turn, provides...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acacia caven; AFLP; GENELAND; DAPC.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100012
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Assessment of genetic diversity in sesame (Sesamum indicum L.) detected by Amplified Fragment Length Polymorphism markers Electron. J. Biotechnol.
Ali,Ghulam M; Yasumoto,Sirato; Seki-Katsuta,Masumi.
Sesame (Sesamum indicum L.) is one of the oldest oil crops and is widely cultivated in Asia and Africa. To determine the level of genetic diversity in relation to geographical origins and morphological characteristics, a total of 96 accessions have been collected from different parts of the world and were analyzed using AFLP techniques. Twenty-one primer pairs generated a total of 445 bands and among them 157 (35%) were polymorphic. Using UPGMA clustering analysis method based on the similarity coefficient, accessions were separated into two major groups. The first group mostly consists of Eastern Asian origin and another group consists of South Asian origin. Sub-clusters separated the accessions and form distinct diversity among groups. Considering the...
Tipo: Journal article Palavras-chave: AFLP; Genetic diversity; Geographical origin; Morphology; Sesame.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100002
Registros recuperados: 107
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