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| Registros recuperados: 94 | |
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| Lallias, Delphine; Beaumont, A.r.; Haley, C.s.; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie. |
| This study presents the first genetic linkage map for the European flat oyster Ostrea edulis. AFLP and 20 microsatellite markers were genotyped in a three-generation pedigree comprised of 2 grand-parents, 2 parents and 92 progeny. Chi-square goodness of fit tests revealed high segregation distortion, which was significant for 32.8% of markers. Sixteen microsatellites and 235 AFLPs (170 AFLPs type 1:1 and 65 AFLPs type 3:1) were used to build sex-specific linkage maps using CriMap software. The first parental map (P1) consisted of 104 markers grouped in nine linkage groups, spanning 471.2 cM with an average spacing of 4.86 cM. The second parental map (P2) consisted of 117 markers grouped in ten linkage groups (which equals the haploid chromosome number),... |
| Tipo: Text |
Palavras-chave: Microsatellite; AFLP; Ostrea edulis; Flat oyster; Genetic linkage map. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2642.pdf |
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| Lallias, Delphine; Hecquet, Céline; Boudry, Pierre; Lapegue, Sylvie; Beaumont, Andy. |
| The blue mussel, Mytilus edulis, is widely distributed in the northern hemisphere, from the White Sea to the Atlantic coast of southern France, and is an economically important species (1 million tons per year, FAO, 2002). At present however, little domestication has been achieved with bivalves, in contrast to agricultural crops, livestock and horticultural species like tomatoes, where selection and production is entirely controlled by man. For bivalves, despite their economic importance, no lines with selected characteristics have been taken to the production scale. There is therefore a need to make up for lost time. Our interest in genetic mapping sprang from some results showing a genetic component to some economically important traits like growth and... |
| Tipo: Text |
Palavras-chave: QTLs; AFLP; Genetic; Mussel; Mytilus edulis; Genetic linkage map. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3465.pdf |
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| Souza,Laís Bérgamo de; Ruas,Eduardo A.; Rodrigues,Luana A.; Ruas,Claudete F.; Ruas,Paulo M.. |
| Parapiptadenia rigida is a tropical early secondary succession tree characteristic of the Tropical Atlantic Rainforest. This species is of great ecological importance in the recovery of degraded areas. In this study we investigated the variability and population genetic structure of eight populations of P. rigida. Five AFLP primer combinations were used in a sample of 159 individuals representing these eight populations, rendering a total of 126 polymorphic fragments. The averages of percentage of polymorphic loci, gene diversity, and Shannon index were 60.45%, 0.217, and 0.322, respectively. A significant correlation between the population genetic variability and the population sizes was observed. The genetic variability within populations (72.20%) was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tropical tree; Genetic diversity; Population genetics; Conservation; AFLP. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400012 |
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| Altinkut,A.; Kazan,K.; Gozukirmizi,N.. |
| The amplified fragment length polymorphism (AFLP) assay is an efficient method for the identification of molecular markers, useful in the improvement of numerous crop species. Bulked Segregant Analysis (BSA) was used to identify AFLP markers associated with water-stress tolerance in barley, as this would permit rapid selection of water-stress tolerant genotypes in breeding programs. AFLP markers linked to water-stress tolerance was identified in two DNA pools (tolerant and sensitive), which were established using selected F2 individuals resulting from a cross between water-stress-tolerant and sensitive barley parental genotypes, based on their paraquat (PQ) tolerance, leaf size, and relative water content (RWC). All these three traits were previously shown... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bulked segregant analysis; Drought; Hordeum vulgare; AFLP; Paraquat. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100013 |
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| Lallias, Delphine; Lapegue, Sylvie; Hecquet, Celine; Boudry, Pierre; Beaumont, A. |
| We report the construction of the first genetic linkage map in the blue mussel, Mytilus edulis. AFLP markers were used in 86 full-sib progeny from a controlled pair mating, applying a double pseudo-test cross strategy. Thirty-six primer pairs generated 2354 peaks, of which 791 (33.6%) were polymorphic in the mapping family. Among those, 341 segregated through the female parent, 296 through the male parent (type 1:1) and 154 through both parents (type 3:1). Chi-square goodness-of-fit tests revealed that 71% and 73% of type 1:1 and 3:1 markers respectively segregated according to Mendelian inheritance. Sex-specific linkage maps were built with mapmaker 3.0 software. The female framework map consisted of 121 markers ordered into 14 linkage groups, spanning... |
| Tipo: Text |
Palavras-chave: Mytilus edulis; Genetic linkage map; Blue mussel; AFLP. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2830.pdf |
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| Espinoza Sánchez,EA; Silos Espino,H; Flores Benitez,S; Valera Montero,LL; Rodríguez Salazar,E; Gallegos Vázquez,C; Guevara Lara,F; González Chavira,M; Guzmán Maldonado,HS. |
| Para contribuir al conocimiento de la diversidad genética del nopal se analizaron 85 genotipos representativos de Opuntia (silvestres y cultivados) por medio de la técnica de AFLPs y características del fruto. El análisis de AFLPs con los iniciadores Mse ICAC/Eco RI-AAG generaron más fragmentos polimórficos (57,5 en promedio) por genotipo; se obtuvo un dendrograma que no mostró una clara relación de acuerdo a la taxonomía conocida. En complemento al perfil genético, y para permitir una referencia morfológica o química directa, agrupamos a los genotipos de acuerdo al contenido de °Brix de sus frutos, debido a que esta es una característica primordial para su identificación visual y aprovechamiento de consumo directo. Se encontró que los genotipos que... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caracterización Molecular; Opuntia; AFLP; Dendrograma; Morfología del fruto. |
| Ano: 2014 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572014000200010 |
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| OLIVEIRA, P. R. D. de; SCOTTON, D. C.; NISHIMURA, D. S.; FIGUEIRA, A.. |
| RESUMO - Com objetivo de estudar diversidade genética e identificar marcadores associados à resistência ao míldio (Plasmopara viticola) e ao oídio (Uncinula necator), foram analisadas as cultivares de videira A 1976, CG 87746, CNPUV 154-27, Crimson Seedless, Gota de Ouro, Itália, Seyve Villard 12327 e Seyve Villard 12375. As análises de polimorfismo de comprimento de fragmento amplificado (AFLP) seguiram as etapas de digestão, ligação, pré-amplificação e amplificação. Efetuou-se a separação dos fragmentos amplificados em gel de 7% de poliacrilamida desnaturante (uréia 7M) e coloração com nitrato de prata. A similaridade foi estimada com base no coeficiente de Jaccard, e a agregação, por UPGMA. Foi gerada uma matriz de similaridade com bom ajustamento da... |
| Tipo: Artigo de periódico |
Palavras-chave: AFLP; Doença de Planta; Marcador Molecular; Míldio; Oídio; Resistência; Uva; Viticultura. |
| Ano: 2005 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/540917 |
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| Busato,Gustavo R.; Grützmacher,Anderson D.; Oliveira,Antônio C. de; Vieira,Eduardo A.; Zimmer,Paulo D.; Kopp,Maurício M.; Bandeira,Juliana de M.; Magalhães,Taís R.. |
| O objetivo do trabalho foi analisar a estrutura e a diversidade molecular de quatro populações de Spodoptera frugiperda (J.E. Smith) associadas às culturas de milho e arroz no Rio Grande do Sul. Foram coletadas lagartas de quatro populações em áreas isoladas (distanciadas em mais de 300 km) nos municípios de Santa Rosa (milho) e Uruguaiana (arroz irrigado) e em áreas adjacentes, no município de Pelotas (milho e arroz irrigado). A análise de 40 lagartas (10 de cada população), com cinco combinações de oligonucleotídios iniciadores geraram o total de 241 locos, dentre os quais 229 (95%) foram polimórficos. O dendrograma gerado pelo método de agrupamento UPGMA, baseado na matriz de similaridade de Dice, mostrou sobreposições entre as lagartas das populações... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; Lagarta-do-cartucho; Lagarta-da-folha; AFLP; Biótipo. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000600008 |
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| GOMES, H. T.. |
| O objetivo do trabalho foi avaliar a utilização de sistemas de imersão temporária na embriogênese somática de dendezeiros (Elaeis guineensis Jacq.), a partir de folhas imaturas de plantas adultas, caracterizar morfoanatômica e bioquimicamente os estádios embriogênicos obtidos no processo, além analisar os clones regenerados por marcadores ISSR e AFLP (MSAP). Num primeiro experimento, calos primários da variedade C2328 foram multiplicados por cinco subcultivos de 30 dias, em quatro diferentes sistemas de cultivo: semi-sólido, líquido sob agitação, além dos biorreatores de imersão temporária R.I.T.A.® e tipo frascos gêmeos (BIT). Em seguida, o biorreator mais eficiente foi avaliado isoladamente na multiplicação de calos de outras três variedades: B35-2932,... |
| Tipo: Teses |
Palavras-chave: Biorreatores de imersão temporária; Embrião somático; Variação somaclonal; ISSR; AFLP; MSAP; Ontogênese.; Micropropagação; Marcador molecular; Histologia; Fisiologia; Morfogênese.; Arecaceae.. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1065516 |
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| Pometti,Carolina L.; Bessega,Cecilia F.; Saidman,Beatriz O.; Vilardi,Juan C.. |
| Bayesian clustering as implemented in STRUCTURE or GENELAND software is widely used to form genetic groups of populations or individuals. On the other hand, in order to satisfy the need for less computer-intensive approaches, multivariate analyses are specifically devoted to extracting information from large datasets. In this paper, we report the use of a dataset of AFLP markers belonging to 15 sampling sites of Acacia caven for studying the genetic structure and comparing the consistency of three methods: STRUCTURE, GENELAND and DAPC. Of these methods, DAPC was the fastest one and showed accuracy in inferring the K number of populations (K = 12 using the find.clusters option and K = 15 with a priori information of populations). GENELAND in turn, provides... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acacia caven; AFLP; GENELAND; DAPC. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100012 |
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| Ali,Ghulam M; Yasumoto,Sirato; Seki-Katsuta,Masumi. |
| Sesame (Sesamum indicum L.) is one of the oldest oil crops and is widely cultivated in Asia and Africa. To determine the level of genetic diversity in relation to geographical origins and morphological characteristics, a total of 96 accessions have been collected from different parts of the world and were analyzed using AFLP techniques. Twenty-one primer pairs generated a total of 445 bands and among them 157 (35%) were polymorphic. Using UPGMA clustering analysis method based on the similarity coefficient, accessions were separated into two major groups. The first group mostly consists of Eastern Asian origin and another group consists of South Asian origin. Sub-clusters separated the accessions and form distinct diversity among groups. Considering the... |
| Tipo: Journal article |
Palavras-chave: AFLP; Genetic diversity; Geographical origin; Morphology; Sesame. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100002 |
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| Registros recuperados: 94 | |
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