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| Albenne, C.; Skov, L.K.; Mirza, O.; Gajhede, M.; Feller, G.; d'Amico, S.; André, G.; Potocki-Véronèse, G.; van der Veen, B.A.; Monsan, P.; Remaud-Simeon, M.. |
| Amylosucrase from Neisseria polysaccharea is a remarkable transglucosidase from family 13 of the glycoside-hydrolases that synthesizes an insoluble amylose-like polymer from sucrose in the absence of any primer. Amylosucrase shares strong structural similarities with α-amylases. Exactly how this enzyme catalyzes the formation of α-1,4-glucan and which structural features are involved in this unique functionality existing in family 13 are important questions still not fully answered. Here, we provide evidence that amylosucrase initializes polymer formation by releasing, through sucrose hydrolysis, a glucose molecule that is subsequently used as the first acceptor molecule. Maltooligosaccharides of increasing size were produced and successively elongated at... |
| Tipo: Journal Article |
Palavras-chave: AMYLOSUCRASE; EVOLUTION MOLECULAIRE DIRIGEE; ALPHA AMYLASE; AMYLOSACCHARASE; INGENIERIE ENZYMATIQUE; NEISSERIA POLYSACCHAREA; NEISSERIACEAE. |
| Ano: 2004 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0500023786108456&uri=/notices/prodinra1/2010/11/ |
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