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Registros recuperados: 6
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Branched-chain fatty acids, increased in tears of blepharitis patients, are not toxic for conjunctival cells Inra
Joffre, C.; Souchier, M.; Leclere, L.; Buteau, B.; Grégoire, S.; Lizard, G.; Montange, T.; Acar, N.; Bron, A.; Creuzot Garcher, C.; Diebold, Y.; Bretillon, L..
Aim: The composition of the meibum of blepharitis patients is characterised by increased levels of branched-chain fatty acids (BCFAs) that return to normal values in patients treated with cyclins and lid hygiene. The aim of this study was to determine if BCFAs had toxic effects on conjunctival cells related to the disease.Methods: Chang and IOBA-NHC conjunctival human cells were treated with BCFAs (isoC16 and isoC20) or palmitic acid as a control for 4 h or 24 h at 50 μM or 100 μM. Morphological and functional changes were investigated by measuring mitochondrial dehydrogenase activity, cell permeability, mitochondrial depolarisation, chromatin condensation, IL-1β and reactive oxygen species production.Results: None of the fatty acids modified the...
Tipo: Journal Article Palavras-chave: DRY EYE; BENZALKONIUM CHLORIDE; EPITHELIAL-CELLS; MUSCLE CELLS; LIPID LAYER; APOPTOSIS; MEMBRANE; MARKER.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20105cdf51fe&uri=/notices/prodinra1/2010/07/
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Chronic psychological stress alters epithelial cell turn-over in rat ileum Inra
Boudry, G.; Jury, J.; Yang, P. C.; Perdue, M. H..
Tipo: Journal Article Palavras-chave: ENTÉROCYTE; PROLIFÉRATION; ÉPITHÉLIUM INTESTINE; ENTEROCYTE; PROLIFERATION; APOPTOSIS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009b845513d&uri=/notices/prodinra1/2010/09/
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Dietary oxysterols induce in vivo toxicity of coronary endothelial and smooth muscle cells Inra
Meynier, A.; Andre, A.; Lherminier, J.; Grandgirard, A.; Demaison, L..
Dietary cholesterol oxidation products (COPs) were reported to exhibit in vitro toxicity toward vascular cells. The aim of this study was to determine whether dietary COPs induce in vivo toxicity toward coronary arteries and to evaluate their effect on the coronary reactivity. Golden Syrian hamsters were fed either a normolipidic diet or a hyperlipidic diet with or without a mixture of COPs (1.4 mg/kg/day). At the end of the feeding periods, cardiac mitochondria and cytosol were prepared to determine the subcellular distribution of cytochrome c. Oxidative phosphorylation was evaluated with glutamate, pyruvate or palmitoylcarnitine as a substrate. The main coronary artery was examined all along its length by transmission electron microscopy (TEM). Plasma...
Tipo: Journal Article Palavras-chave: DIETARY OXYSTEROLS; CORONARY CELLS; TOXICITY; APOPTOSIS.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008bd62c13e&uri=/notices/prodinra1/2008/11/
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Influence of bovine colostrum on restoration of intestinal mucosa in weaned piglets Inra
Huguet, A.; Le Normand, L.; Fauquant, J.; Kaeffer, B.; Le Huëron-Luron, I..
The immediate post-weaning period characterized by reduced feed intake induces gut structural alterations. A rapid mucosarestoration is essential for an optimal growth recovery in piglets. Bovine colostrum has been shown to prevent villous atrophy inmice and to stimulate mucosal healing in patients suffering from inflammatory gut disease. Therefore we evaluated the influence of bovine colostrum on mucosa restoration in weaned piglets. In trial 1, twenty-four piglets weaned at 21 days of age were fed acontrol diet or a diet supplemented with bovine colostrum for 7 or 14 days. Duodenal villous sizes and crypt cell proliferation were analysed. In trial 2, three piglets weaned at 28 days of age were fasted for 48 h. At slaughter duodenal explants were prepared...
Tipo: Journal Article Palavras-chave: COLOSTRUM; ARN; APOPTOSIS; GUT RESTORATION; CELL PROLIFERATION; WEANING; PIGLETS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008730b7296&uri=/notices/prodinra1/2008/04/
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Recent insight into the pathogenicity mechanisms of the emergent pathogen Photorhabdus asymbiotica Inra
Costa, S.C.P.; Chavez, C.V.; Jubelin, G.; Givaudan, A.; Escoubas, J.M.; Brehélin, M.; Zumbihl, R..
Photorhabdus asymbiotica is unique among the entomopathogenic bacteria of this genus in also being able to infect humans, leading to its isolation from some clinical samples. Recent comparative genomics data and the results of studies of interactions between bacteria and cells provide insight into the adaptation of this bacterium to its new niche, the human body
Tipo: Journal Article Palavras-chave: PHOTORABDUS ASYMBIOTICA; EMERGENT PATHOGEN; INSECT; MAN; COMPARATIVE GENOMICS; PHAGOCYTOSIS; APOPTOSIS; VIRULENCE FACTORS.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010764fee76&uri=/notices/prodinra1/2010/10/
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The cyclomodulin Cif of Photorhabdus luminescens inhibits insect cell proliferation and triggers host cell death by apoptosis Inra
Varela Chavez, C.; Jubelin, G.; Courties, G.; Gomard, A.; Ginibre, N.; Pages, S.; Taieb, F.; Girard, P.A.; Oswald, E.; Givaudan, A.; Zumbihl, R.; Escoubas, J.M..
Cycle inhibiting factors (Cif) constitute a broad family of cyclomodulins present in bacterial pathogens of invertebrates and mammals. Cif proteins are thought to be type III effectors capable of arresting the cell cycle at G2/M phase transition in human cell lines. We report here the first direct functional analysis of CifPl, from the entomopathogenic bacterium Photorhabdus luminescens, in its insect host. The cifPl gene was expressed in P. luminescens cultures in vitro. The resulting protein was released into the culture medium, unlike the well characterized type III effector LopT. During locust infection, cifPl was expressed in both the hemolymph and the hematopoietic organ, but was not essential for P. luminescens virulence. CifPl inhibited...
Tipo: Journal Article Palavras-chave: PHOTORHABDUS; APOPTOSIS; CYCLE INHIBITING FACTOR; CIF; TYPE III SECRETION SYSTEM.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20119b3aacae&uri=/notices/prodinra1/2011/05/
Registros recuperados: 6
Primeira ... 1 ... Última
 

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