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Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens 55
Hung,Hung-Chang; Chan,Chi-Ho; Tsao,Shih-Ming; Chen,Shiuan-Chih; Wu,Tzu-Chin; Lu,Min-Chi; Wu,Der-Jinn; Huang,Shu-Ju; Lee,Yuan-Ti.
OBJECTIVE: The diagnostic efficacy of the BDProbeTEC ET Mycobacterium tuberculosis (MTB) complex direct detection assay (DTB) performed on bronchoalveolar lavage (BAL) specimens and sputum smears was compared with acid-fast bacilli (AFB) smear microscopy. METHOD: AFB smear microscopy, DTB and culture results of 286 patients with pulmonary tuberculosis were retrospectively reviewed. A total of 120 patients provided expectorated sputum samples, and 166 patients provided BAL specimens. Culture results and clinical diagnosis were used as gold standards. RESULTS: The sensitivity and specificity of the DTB assay in detecting MTB in sputum specimens was significantly higher compared to AFB smear microscopy (83.7% and 82.4%, vs. 75.6%, and 41.2%, respectively)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bronchoalveolar lavage; Mycobacterium tuberculosis; Acid-fast bacilli staining; Molecular diagnosis; BDProbeTEC ET system.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300005
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