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Registros recuperados: 6
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Comparison of haploid and doubled haploid sugar beet clones in their ability to micropropagate and regenerate Electron. J. Biotechnol.
Klimek-Chodacka,Magdalena; Baranski,Rafal.
Background: Haploid plant material is considered as recalcitrant to organogenesis, propagation, and maintenance in vitro. However, sugar beet (Beta vulgaris L.) breeders utilizing doubled haploid (DH) technology in their breeding programs indicate that sugar beet haploids may be cultured in vitro as well as diploids. Thus in this paper the in vitro performance of haploid and the doubled haploid sugar beet of various origin was evaluated. The DHs were derived from haploids by diploidization and twelve such haploid and corresponding DH clone pairs were obtained thus the comparison included haploid and DH clones that had identical allelic composition and differed only in their ploidy level. Results: The genotypes differed in shoot morphology and...
Tipo: Journal article Palavras-chave: Adventitious buds; Axillary shoots; Beta vulgaris; Organogenesis; Ploidy.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200001
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In vitro organogenesis from internodal segments of adult sweet orange plants. Repositório Alice
BASSAN, M. M.; MOURÃO FILHO, F. de A. A.; MIYATA, L. Y.; MENDES, B. M. J..
The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27ºC in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and Valência was promoted by EME supplemented with BAP, while NAA showed no apparent effect.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Citrus sinensis; Adventitious buds; Biotechnology; Plant growth regulators.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/901813
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In vitro organogenesis from internodal segments of adult sweet orange plants PAB
Bassan,Meire Menezes; Mourão Filho,Francisco de Assis Alves; Miyata,Luzia Yuriko; Mendes,Beatriz Madalena Januzzi.
The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27ºC in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and Valência was promoted by EME supplemented with BAP, while NAA showed no apparent effect.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Citrus sinensis; Adventitious buds; Biotechnology; Plant growth regulators.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000600015
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In vitro organogenesis of zucchini squash cv. Caserta Horticultura Brasileira
Stipp,Liliane Cristina L; Monteiro-Hara,Alessandra Cristina BA; Mendes,Beatriz Madalena J.
A protocol for the in vitro culture of Cucurbita pepo cv. Caserta was studied, using a cotyledon segment with an attached hypocotyl fragment as an explant. First, to determine the optimal seedling age, explants were collected from 4 to 6-day-old in vitro germinated seedlings and cultured in MS basal medium supplemented with benzylaminopurine (BAP, 4.5 µM), under a 16-h photoperiod at 27ºC. Based on the results obtained, the explants collected from the 4-day-old seedlings were then cultured in MS basal medium supplemented with different concentrations of BAP (0, 1.1, 2.2, 3.3, 4.5, or 5.5 µM) and incubated under a 16-h photoperiod at 27ºC. In vitro organogenesis was most efficient with explants collected from 4-day-old seedlings cultured in medium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adventitious buds; Cotyledon attached to hypocotyl; Cucurbita pepo; In vitro culture.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000200015
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Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis dehn and histological study of organogenesis in vitro BABT
Dibax,Roberson; Quisen,Regina Caetano; Bona,Cleusa; Quoirin,Marguerite.
The present work aimed at regenerating plants of Eucalyptus camaldulensis from the cotyledonary explants and describing the anatomy of the tissues during callogenesis and organogenesis processes, in order to determine the origin of the buds. The cotyledonary leaves of E. camaldulensis were cultured in Murashige and Skoog (MS), WPM and JADS media supplemented with 2.7 µM NAA and 4.44 µM BAP. The best results for bud regeneration were obtained on MS and WPM media (57.5 and 55% of calluses formed buds, respectively). Shoot elongation and rooting (80%) were obtained on MS/2 medium (with half-strength salt concentration) with 0.2% activated charcoal. Acclimatization was performed in the growth chamber for 48 h and then the plants were transferred to a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adventitious buds; Callogenesis; Organogenesis; Tissue culture; Anatomical study.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200009
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Tissue culture of Cecropia glaziovii Sneth (urticaceae): vegetative micropropagation and plant regeneration from callus Ciência e Agrotecnologia
Alves,Marcos Nopper.
Cecropia glaziovii is a tree with used in Brazilian popular medicine. Methods allowing the clonal propagation of this species are of great interest for superior genotype multiplication and perpetuation. For this reason, we examined the effect of different culture media and different types of explants on adventitious shoot regeneration from callus and buds of C. glaziovii. Leaves, petioles and stipules obtained from aseptically grown seedlings or from pre-sterilized plants were used to initiate cultures. Adventitious shoot regeneration was achieved when apical and axillary buds were inoculated on gelled Murashige & Skoog (MS) medium supplemented with 6-benzylaminopurine alone (BAP) (1.0, 5.0 or 10.0 mg L-1) or combined with -naphthalene acetic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Clonal propagation; Adventitious buds; Growth regulators; Callogenesis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500024
Registros recuperados: 6
Primeira ... 1 ... Última
 

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