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| Santisi,Santina; Cappello,Simone; Catalfamo,Maurizio; Mancini,Giuseppe; Hassanshahian,Mehdi; Genovese,Lucrezia; Giuliano,Laura; Yakimov,Michail M.. |
| Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas: Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source). Measures of total and live... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alcanivorax; Pseudomonas; Rhodococcus; Bioremediation; Bioaugmentation. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200377 |
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| Tapilatu, Y.; Acquaviva, M.; Guigue, C.; Miralles, G.; Bertrand, J.-c.; Cuny, P.. |
| Aims: To isolate and identify alkane-degrading bacteria from deep-sea superficial sediments sampled at a north-western Mediterranean station. Methods and Results: Sediments from the water/sediment interface at a 2400 m depth were sampled with a multicorer at the ANTARES site off the French Mediterranean coast and were promptly enriched with Maya crude oil as the sole source of carbon and energy. Alkane-degrading bacteria belonging to the genera Alcanivorax, Pseudomonas, Marinobacter, Rhodococcus and Clavibacter-like were isolated, indicating that the same groups were potentially involved in hydrocarbon biodegradation in deep sea as in coastal waters. Conclusions: These results confirm that members of Alcanivorax are important obligate alkane degraders in... |
| Tipo: Text |
Palavras-chave: Alcanivorax; Alkane-degrading bacteria; Deep-sea sediments. |
| Ano: 2010 |
URL: https://archimer.ifremer.fr/doc/00303/41417/40607.pdf |
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| Cappello,Simone; Genovese,Maria; Denaro,Renata; Santisi,Santina; Volta,Anna; Bonsignore,Martina; Mancini,Giuseppe; Giuliano,Laura; Genovese,Lucrezia; Yakimov,Michail M.. |
| Oil spill microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS2003) on quick stimulation of hydrocarbonoclastic bacteria. Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS2003 (SW+OIL+EPS2003); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL). Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out. Community composition of marine bacterioplankton was determined by 16S rRNA gene clone libraries. Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alcanivorax; Bioemulsificant exopolysaccharide (EPS2003); Oil spill. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400023 |
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