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Actividad antioxidante de alcaloides de Erythrina americana Miller. Colegio de Postgraduados
Ibarra Estrada, Emmanuel.
Los alcaloides son compuestos que presentan efectos fisiológicos y propiedades farmacológicas a bajas concentraciones. Los objetivos de este estudio fueron extraer y aislar fracciones crudas de alcaloides y el compuesto erisodina de semillas de E. americana, estimar sus rendimientos y evaluar la actividad antioxidante de fracciones crudas de alcaloides y del compuesto puro erisodina. La actividad antioxidante de las fracciones de alcaloides libres hexánicos, libres metanólicos, liberados metanólicos y de erisodina se evaluó mediante el método del radical estable 2,2-difenil-1-picrilhidrazilo (DPPH) que al reaccionar con un antirradical sufre una decoloración de azul-violeta a amarillo; dicha reacción se midió espectrofotométricamente. Entre las fracciones...
Palavras-chave: Radical libre; Antirradical; Erisodina; Alcaloides; Erythrina americana; Free radical; Antiradical; Erysodine; Alkaloids; Maestría; Fisiología Vegetal.
Ano: 2010 URL: http://hdl.handle.net/10521/221
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Actividad antioxidante de alcaloides de Erythrina americana Miller. Colegio de Postgraduados
Ibarra Estrada, Emmanuel.
Los alcaloides son compuestos que presentan efectos fisiológicos y propiedades farmacológicas a bajas concentraciones. Los objetivos de este estudio fueron extraer y aislar fracciones crudas de alcaloides y el compuesto erisodina de semillas de E. americana, estimar sus rendimientos y evaluar la actividad antioxidante de fracciones crudas de alcaloides y del compuesto puro erisodina. La actividad antioxidante de las fracciones de alcaloides libres hexánicos, libres metanólicos, liberados metanólicos y de erisodina se evaluó mediante el método del radical estable 2,2-difenil-1-picrilhidrazilo (DPPH) que al reaccionar con un antirradical sufre una decoloración de azul-violeta a amarillo; dicha reacción se midió espectrofotométricamente. Entre las fracciones...
Palavras-chave: Radical libre; Antirradical; Erisodina; Alcaloides; Erythrina americana; Free radical; Antiradical; Erysodine; Alkaloids; Maestría; Fisiología Vegetal.
Ano: 2010 URL: http://hdl.handle.net/10521/221
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Ajmalicine Bioproduction in Catharanthus Roseus (L) G. Don Inoculated with Arbuscular Mycorrhiza and Fertilized with Nitrogen Rev. Bras. Ciênc. Solo
Monnerat,Cecília Silva; Freitas,Marta Simone Mendonça; Vieira,Ivo José Curcino; Martins,Marco Antônio; Carvalho,Almy Junior Cordeiro de; Santos,Paulo Cesar dos; Lima,Thaísa Capato.
ABSTRACT Catharanthus roseus (L) G. Don (Madagascar periwinkle) belongs to the Apocynaceace family and is widely spread throughout tropical and subtropical regions of the world. The plant produces several important alkaloids, such as ajmalicine and serpentine, which are used in the treatment of circulatory diseases. The potential of inoculation with arbuscular mycorrhizal fungi (AMF) and nitrogen fertilization to enhance the production of alkaloids was investigated in periwinkle. A greenhouse experiment was carried out to evaluate the effects of arbuscular mycorrhizal fungi and N fertilizer dosages on plant growth, production of ajmalicine, and nutrient content in roots. The concentration of ajmalicine was determined by reverse-phase high-performance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Periwinkle; Apocynaceae; Alkaloids; Plant nutrition.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832018000100416
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Alkaloids in Solanum torvum Sw (Solanaceae): (With 2 Tables & 1 Figure) Phyton
Pérez-Amador,MC; Muñoz Ocotero,V; García Castañeda,JM; González Esquinca,AR.
A comparison was made between plants of Solanum torvum Sw that grow in Chiapas, Mexico, and plants of the same species originating from India. This was effected to establish either similarities or differences between these plants in total alkaloid contents and presence of solasodine, an important alkaloid for the partial synthesis of steroids. The total alkaloid content (0.12%) of the plants coming from Chiapas and India was the same. However, solasodine was found only in the plants of Chiapas. In addition, the total amount of glycoalkaloids (0.038%) and two glycosilated compounds derived from solasodine, solasonine (0.0043%) and solamargine (0.0028%), were determined.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanaceae; Solanum torvum; Alkaloids; Solasodine; Solamargine.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572007000100004
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Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook BJPS
Emir,Ahmet; Emir,Ceren; Bozkurt,Buket; Onur,Mustafa Ali; Somer,Nehir Unver; Kaya,Gulen Irem.
ABSTRACT In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut(r)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5, v/v/v) applied at a flow rate 1 mL min-1 using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaloids; Amaryllidaceae; Plant extracts; High Performance Liquid Chromatography/method validation; High Perfomance Liquid Chromatography/quantitative analysis..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100603
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ASSESSMENT OF BENZOPHENANTHRIDINE AND PROTOPINE ALKALOIDS IN BROILER CHALLENGED AND NOT BY SALMONELLA HEIDELBERG Rev. Bras. Ciênc. Avic.
Previato do Amaral,PFG; Otutumi,LK; Rodrigues,GV; Lima,ET; Fernandes,JIM; Vendrame,A; Mezalira,TS; Suenaga,SS; Sestari,DAO; Cestari,IED; Martins,LA.
ABSTRACT Salmonellosis is a globally important zoonosis, and Salmonella Heidelberg is one of the most prevalent serovars in poultry production worldwide, as well as in food poisoning cases. Antimicrobial drugs were previously widely used to face health challenges in animal production; however, since their ban as performance enhancers, many alternative strategies have been proposed. One of these strategies is the use of plant extracts, such as those containing the alkaloids benzophenanthridine and protopine. These compounds have antimicrobial, anti-inflammatory, immunomodulation, and nutritional effects. The objective of the present study was to evaluate the effects of the supply of a product containing benzophenanthridine and protopine (Sangrovit(r)WS 100...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaloids; Broilers; Intestinal morphology; Performance; Sanguinarine.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000300525
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Bromopyrrole alkaloids from the Caribbean sponge Agelas cerebrum OceanDocs
Regalado, E.L.; Laguna, A.; Mendiola, J.; Thomas, O.P.; Nogueiras, C..
Bioguided fractionation of Agelas cerebrum crude extract resulted in isolation of four bromopyrrole and four bromopyrrole aminoimidazole alkaloids, identified as 5-bromopyrrole-2-carboxylic acid (1), 4-bromopyrrole-2-carboxylic acid (2), 3,4-bromopyrrole-2-carboxylic acid (3), 4,5-bromopyrrole-2-carboxylic acid (4), oroidin (5), bromoageliferin (6), dibromoageliferin (7) and dibromosceptrin (8) on the basis of spectroscopic data analyses (UV, IR, HRMS, 1D and 2D NMR) and comparison with literature data. This is the first report of compounds 2 and 3 in a marine sponge belonging to the Agelas genus and the first evidence of the presence of 1 from a natural source.
Tipo: Journal Contribution Palavras-chave: Alkaloids; Sponges; Chemistry; Chemistry; Alkaloids; Sponges; Http://aims.fao.org/aos/agrovoc/c_1522; Http://aims.fao.org/aos/agrovoc/c_269; Http://aims.fao.org/aos/agrovoc/c_7321.
Ano: 2010 URL: http://hdl.handle.net/1834/3862
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Bufotenine is able to block rabies virus infection in BHK-21 cells J. Venom. Anim. Toxins incl. Trop. Dis.
Vigerelli,Hugo; Sciani,Juliana Mozer; Jared,Carlos; Antoniazzi,Marta Maria; Caporale,Graciane Maria Medeiros; Silva,Andréa de Cássia Rodrigues da; Pimenta,Daniel C.
Background Rabies is a fatal zoonotic neglected disease that occurs in more than 150 countries, and kills more than 55.000 people every year. It is caused by an enveloped single stranded RNA virus that affects the central nervous system, through an infection initiated by the muscular nicotinic acetylcholine receptor, according to many authors. Alkaloids, such as acetylcholine, are widespread molecules in nature. They are present in numerous biological fluids, including the skin secretion of many amphibians, in which they act (together with proteins, peptides and steroids) as protection agents against predators and/or microorganisms. Among those amphibians that are rich in alkaloids, there is the genus Rhinella.Methods Bufotenine was isolated from Rhinela...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabies; Rhinella; Bufotenine; Alkaloids; Toxins; Mass spectrometry.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200336
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Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts Anais da ABC (AABC)
PINTO,NÍCOLAS C.C.; SILVA,JUCÉLIA B.; MENEGATI,LAURA M.; GUEDES,MARIA CLARA M.R.; MARQUES,LUCAS B.; SILVA,THIAGO P. DA; MELO,ROSSANA C.N. DE; SOUZA-FAGUNDES,ELAINE M. DE; SALVADOR,MARCOS J.; SCIO,ELITA; FABRI,RODRIGO L..
ABSTRACT This study aimed to further investigate the cytotoxicity against tumor cell lines and several bacterial strains of Annona squamosa and its mode of action. Methanol extracts of A. squamosa leaves (ASL) and seeds (ASS) were used. ASL showed significant antibacterial activity against S. aureus, K. pneumoniae and E. faecalis with MIC values of 78, 78 and 39 µg/mL respectively. Moreover, ASL exhibited significant biofilm disruption, rapid time dependent kinetics of bacterial killing, increased membrane permeability and significantly reduced the cell numbers and viability. Regarding the cytotoxicity against tumor cell lines, ASS was more active against Jurkat and MCF-7 cells, with CI50 1.1 and 2.1 µg/mL, respectively. ASL showed promising activity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaloids; Annona squamosa; Antibacterial; Citotoxicity; Herbal medicine.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502053
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Development and validation of a UPLC-ESI-MS method for quantitation of the anti-Alzheimer drug galantamine and other Amaryllidaceae alkaloids in plants. Repositório Alice
PAIVA, J. R. DE; SOUZA, A. S. DE Q.; PEREIRA, R. de C. A.; RIBEIRO, P. R. V.; ZOCOLO, G. J.; BRITO, E. S. de; PESSOA, O. D. L.; CANUTO, K. M..
Tipo: Artigo de periódico Palavras-chave: Mal de Alzheimer; Galantamina; UPLC-ESI-MS; Alcalóide; Amaryllidaceae; Alkaloids.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118088
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Developmental Methylome of the Medicinal Plant Catharanthus roseus Unravels the Tissue-Specific Control of the Monoterpene Indole Alkaloid Pathway by DNA Methylation ArchiMer
Dugé De Bernonville, Thomas; Maury, Stéphane; Delaunay, Alain; Daviaud, Christian; Chaparro, Cristian; Tost, Jörg; O’connor, Sarah Ellen; Courdavault, Vincent.
Catharanthus roseus produces a wide spectrum of monoterpene indole alkaloids (MIAs). MIA biosynthesis requires a tightly coordinated pathway involving more than 30 enzymatic steps that are spatio-temporally and environmentally regulated so that some MIAs specifically accumulate in restricted plant parts. The first regulatory layer involves a complex network of transcription factors from the basic Helix Loop Helix (bHLH) or AP2 families. In the present manuscript, we investigated whether an additional epigenetic layer could control the organ-, developmental- and environmental-specificity of MIA accumulation. We used Whole-Genome Bisulfite Sequencing (WGBS) together with RNA-seq to identify differentially methylated and expressed genes among nine samples...
Tipo: Text Palavras-chave: Alkaloids; DNA methylation; Epigenetics; Folivory; Organs; Plant specialized metabolism.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00652/76453/77543.pdf
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Differences in wing melanization and pigmentation pattern in Drosophila buzzatii (Diptera: Drosophilidae) under chemical stress Rev. Soc. Entomol. Argent.
Mongiardino Koch,Nicolás; Hasson,Esteban; Soto,Ignacio M..
Recently, the genetic basis and developmental mechanisms underlying the process of melanization have become progressively elucidated, allowing us to study the evolutionary processes that govern the huge variability of pigmentation observed in nature. However, environmental factors contributing to such variability have received little attention, even though they might have profound ecological consequences. Here we describe a method for analyzing the wing melanization patterns of drosophilids in both qualitative and quantitative ways. We test this method on wings of Drosophila buzzatii Patterson & Wheeler individuals, reared in control and alkaloid-enriched mediums. The alkaloids were extracted from the secondary host of these flies, Echinopsis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila buzzatii; Melanin; Ecotoxicology; Alkaloids; Stress.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802012000100008
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Different leaf extracts from Brunfelsia uniflora in mice Ciência Rural
Carvalho,Ciro José Sousa de; Melo,Marília Martins; Michell,Ana Flávia Ribeiro Machado; Torres,Bruno Benetti Junta; Riet-Correa,Franklin; Guedes,Roberto Maurício Carvalho; Ferraz,Vany Perpetua; Soto-Blanco,Benito; Silva,Silvana Maria Medeiros de Sousa.
ABSTRACT: In Brazil, at least 16 poisonous plant species can affect the central nervous system of livestock. Recently in the state of Piauí, Northeastern Brazil, Brunfelsia uniflora was reported as a cause of nervous signs in donkeys, cattle, and small ruminants. In order to assess the toxicity of B. uniflora extracts, 20 Swiss mice were distributed into four groups (n=5) receiving by gavage alkaloids, flavonoids, saponins, or saline (control group). After administration of the extracts in a single dose (5g kg-1), all mice were observed daily for clinical signs. Mice that received the extracts showed moderate to severe clinical signs, including piloerection, vocalization, and seizures. All mice dosed with saponins died between 10 and 20min after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxicity; Alkaloids; Saponins; Flavonoids; Brunfelsia; Solanaceae.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000100552
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Ergot Disease of Pearl Millet Open Agri
R.P..
Palavras-chave: Millets; Hybrids; Alkaloids; Agronomic characters; Self pollination; Fungi; Grain; Yield losses; Cultural control; Integrated control.
Ano: 1990 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4083
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Estrategias nutricionales para mejorar la respuesta productiva de cerdos en engorda. Colegio de Postgraduados
Martínez Aispuro, José Alfredo.
El presente trabajo tuvo como objetivo reducir y/u optimizar la concentración de algunos nutrimentos en las dietas de cerdos de engorda que puede resultar benéfica en la formulación de raciones. Se realizaron 3 experimentos para evaluar el nivel de lisina, el uso de alcaloides y xilanasas en dietas para cerdos de engorda. En el experimento 1 se determinaron los niveles óptimos biológicos (NOB) de lisina total para cerdos en las etapas de crecimiento y finalización en el comportamiento productivo, las características de la canal, y la concentración de urea en plasma. El NOB para ganancia diaria de peso (GDP), conversión alimenticia, y ganancia de carne magra fue 0.88% de lisina total; mientras que el NOB para consumo de alimento fue 0.961% de lisina total....
Palavras-chave: Lisina; Alcaloides; Dietas con baja proteína; Xilanasas; Energía; Proteína; Lysine; Alkaloids; Low protein diets; Xylanase; Energy; Protein; Ganadería; Doctorado.
Ano: 2013 URL: http://hdl.handle.net/10521/2159
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ESTs from seeds to assist the selective breeding of Jatropha curcas L. for oil and active compounds. Repositório Alice
GOMES, K. A.; ALMEIDA, T. C.; GESTEIRA, A. da S.; LÔBO, I. P.; GUIMARÃES, A. C. R.; MIRANDA, A. B. de; VAN SLUYS, M. A.; CRUZ, R. S. da; CASCARDO, J. C. M.; CARELS, N..
We report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality $10. We found low levels of sequence redundancy and extensive metabolic coverage by homology comparison to GO. After comparison of 5841 non-redundant ESTs from a total of 13193 reads from GenBank with KEGG, we identified tags with nucleotide variations among J. curcas accessions for genes of fatty acid, terpene, alkaloid, quinone and hormone pathways of biosynthesis. More specifically, the expression level of four genes...
Tipo: Separatas Palavras-chave: Biofuel; Terpenes; Pinhão manso; Melhoramento genetico vegatal.; Biocombustível; Jatropha Curcas.; Alkaloids; Fatty acids; Genomics..
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/865928
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Evaluation of the effects of Uncaria rhynchophylla alkaloid extract on LPS-induced preeclampsia symptoms and inflammation in a pregnant rat model BJMBR
Wu,Liang-Zhi; Xiao,Xiao-Min.
Excessive pro-inflammatory cytokines result in adverse pregnancy outcomes, including preeclampsia-like phenotypes, and fetal growth restriction. Anti-inflammation might be an effective therapy. The aim of this research was to investigate whether Uncaria rhynchophylla alkaloid extract (URE), a highly safe anti-inflammation constituent of the herb, can inhibit inflammation and improve clinical characteristics of preeclampsia in a lipopolysaccharide (LPS)-induced preeclampsia rat model. The rat model was established by daily administration of LPS (1 μg/kg body weight per day) from gestational day (GD) 14 to 19. Different doses of URE (35, 70, and 140 mg/kg body weight per day) were administered from GD 14 to GD 19. The effects of URE on proteinuria, maternal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Preeclampsia; Inflammation; Uncaria rhynchophylla; Alkaloids.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600602
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Ganho de peso de alterações anatomopatológicas de tilápia-do-nilo arraçoadas com farelo de cacau. Repositório Alice
PEZZATO, L. E.; OLIVEIRA, A. C. B. de; DIAS, E.; BARROS, M. M.; PEZZATO, A. C..
O experimento foi desenvolvido no Laboratorio de Nutricao de Peixes (F.M.V.Z.), Campus de Botucatu, unidade integrada ao Centro de Aquicultura da UNESP, com o objetivo de avaliar o emprego de farelo de cacau (Theobroma cacao) em dietas para alevinos de tilapia-do-nilo (Oreochromis niloticus) Os alevinos foram alimentados com dietas que continham niveis crescentes de farelo de cacau (FC): 0% FC; 4% FC; 12% FC; 16% FC e 20% FC, por 120 dias, em experimento inteiramente casualizdo (tres repeticoes). O farelo de cacau, nos niveis testados, embora nao afete o ganho de peso (p>0,05), deve ser empregado com restricoes ao compor dieta, pois tanto pode exercer efeito deleterio sobre o figado dos peixes, como pode estimular alteracoes comportamentais da...
Tipo: Artigo de periódico Palavras-chave: Alcaloides; Feeding.; Alimentação; Dieta; Piscicultura; Aquicultura.; Aquaculture; Alkaloids; Diet..
Ano: 1996 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/104091
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Ipomoea brasiliana poisoning on buck reproduction Ciência Rural
Cunha,Ana Lizia Brito da; Medeiros,Juliana Pinto de; Riet-Correa,Franklin; Gardner,Dale; Chaves,Hisadora Advíncula da Silva; Silva Filho,Givaldo Bom da; Souza,Francisco de Assis Leite; Evêncio Neto,Joaquim; Mendonça,Fábio de Souza.
ABSTRACT: Swainsonine-containing plants comprise a group of important poisonous plants in Brazil. This research aimed to characterize both the behavioral changes related to reproduction and appearance of lesions in the reproductive system of bucks poisoned by Ipomoea brasiliana. I. brasiliana plants were collected and administered at a dose of 4g/kg (800µg swainsonine/kg) to two groups of bucks for 45 days. Goats from Group I were euthanized on the 46th day of the experiment, and goats from Group II were euthanized on the 120th day. Group III was composed of goats that did not receive I. brasiliana and were euthanized on the 120th day of the experiment. Reproductive behavioral changes were observed starting on day 20 and were characterized by an absence...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaloids; Lysosomal storage disease; Plant poisoning; Ruminants.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000552
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Mortality of Plutella xylostella larvae treated with Aspidosperma pyrifolium ethanol extracts PAB
Trindade,Roseane Cristina Prédes; Silva,Paulo Pedro da; Araújo-Júnior,João Xavier de; Lima,Ivanildo Soares de; Paula,José Elias de; Sant'Ana,Antônio Euzébio Goulart.
The objective of this work was to assess the effects of Aspidosperma pyrifolium ethanol extracts on cabbage moth (Plutella xylostella) larvae. The ethanol extracts of the stem bark, fruits and roots of A. pyrifolium were obtained by classical phytochemical methods, and the resulting subfractions were tested on P. xylostella, using 4 and 5 mg L-1. The crude ethanol extract of the stem bark was more lethal. The alkaloid-rich aqueous subfraction derived from the stem bark extract caused 100% larval mortality at 4 mg L-1. Insecticidal activity was associated with the presence of the monoterpenoid indole alkaloids aspidofractinine, 15-demethoxypyrifoline, and N-formylaspidofractinine. These alkaloids presented excellent insecticidal properties against P....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaloids; Cabbage moth; Insecticidal activity; Organic extracts; Pereiro-do-sertão.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001200024
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