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Antifibrotic effects of total or partial application of amniotic membrane in hepatic fibrosis Anais da ABC (AABC)
MAMEDE,KARINA M.; SANT’ANNA,LUCIANA B..
Abstract: Liver fibrosis is the final common pathway of chronic liver diseases, having cirrhosis as a possible progression, which has liver transplantation as the only effective treatment. Human amniotic membrane represents a potential strategy as a therapy for liver fibrosis, due to its anti-inflammatory, anti-fibrotic and immunomodulatory properties. The aim of this study was to evaluate amniotic membrane effects as a treatment for hepatic fibrosis induced in rats by bile duct ligation (BDL), verifying alterations between two different forms of amniotic membrane application, around all the lobes of the liver and around only one lobe of the liver. Two weeks after inducing fibrosis, an amniotic membrane fragment was applied to the surface of the liver,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amniotic membrane; Histopathology; Immunohistochemistry; Liver fibrosis; Myofibroblasts.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500702
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Histopathological and Immunohistochemical Changes in the Amniotic Membrane of Gestational Diabetic Mothers International Journal of Morphology
Togrul,Cihan; Görkem,Ümit; Coskun,Bugra; Nergisli,Cansu Kübra; Güngör,Tayfun; Deveci,Engin.
The aim of this study is to examine the changes in the amniotic membrane diagnosed with gestational diabetes mellitus. In this study, as a control group human amnion membrane from normotensive pregnancies was collected from diabetic women at 28­35 weeks of gestation. Gestational diabetes (n= 6) and normal amnion membrane (n= 6) for a total of 12 units were received. Amniotic membrane thickness was measured (p<0.0001) and it was significantly higher in GDM groups compared to control group. The diameter of the amniotic epithelial cell nuclei was measured (p=0.0022). Gestational diabetes results show that there was weakening between amniotic epithelial cell-cell junction. This study showed that structural changes in epithelial cells of amniotic membrane...
Tipo: Journal article Palavras-chave: Amniotic membrane; Gestational diabetic mothers; Immunohistochemical changes.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100030
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Lamellar keratoplasty in rabbits using an allogeneic free omental graft and omentum-derived mesenchymal cells associated with the canine amniotic membrane Ciência Rural
Barros,Séfora Vieira da Silva Gouvêa de; Aldrovani,Marcela; Lacerda,Luciana Cenço Correa de; Horr,Mônica; Marinho,Fábio Andrade; Lima,Tiago Barbalho; Silveira,Camila Pinho Balthazar da; Brito,Fábio Luiz da Cunha; Bassaneze,Vinícius; Flecher,Mayra Cunha; Beltrame,Renato Travassos; Nakamuta,Juliana Sanajotti; Krieger,José Eduardo; Laus,José Luiz; Oliveira,Krishna Duro de.
ABSTRACT: The objective of this research was to evaluate the clinical and microscopic effects in rabbits of lamellar keratoplasty using allogeneic omentum associated with canine amniotic membrane (AM). Rabbits were divided into two groups: one received the allogeneic free omental graft covered with the AM (OM-graft group), while the other received the AM graft containing omental mesenchymal cells (OM-cell group). Clinical signs were evaluated on different postoperative days. After the clinical assessments, the rabbits were euthanized and their corneas were obtained for histopathology and immunohistochemistry (Ki-67, marker for proliferation). Both groups showed chemosis, blepharospasm, eye discharge, hyperemia, and corneal opacity/edema. Neovascularization...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amniotic membrane; Cornea; Omentum; Progenitor cells; Wound healing..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001838
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Long-term effects of human amniotic membrane in a rat model of biliary fibrosis BJMBR
Sant'Anna,L.B.; Brito,F.S.; Barja,P.R.; Nicodemo,M.C..
Liver fibrosis is the most common outcome of chronic liver diseases, and its progression to cirrhosis can only be effectively treated with liver transplantation. The amniotic membrane (AM) has been studied as an alternative therapy for fibrosis diseases mainly for its favorable properties, including anti-inflammatory, anti-scaring and immunomodulatory properties. It was recently demonstrated that the AM reduces the progression of biliary fibrosis to its advanced stage, cirrhosis, when applied on the liver for 6 weeks after fibrosis induction. Here, we investigated the effects of AM on rat fibrotic liver, during a prolonged period of time. Fibrosis was induced by bile duct ligation (BDL), and at the same time, a fragment of AM was applied around the liver....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amniotic membrane; Bile duct ligation; Liver fibrosis; Long-term; Quantitative image analysis; Rat.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700611
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Ultrastructural study of the neovagina following the utilization of human amniotic membrane for treatment of congenital absence of the vagina BJMBR
We present an ultrastructural study of the utilization of human amniotic membrane in the treatment of congenital absence of the vagina in 10 patients. All patients were surgically treated with application of an amniotic membrane graft using the modified McIndoe and Bannister technique. Sixty days after surgery, samples of the vaginal neo-epithelium were collected for transmission electron microscopy analysis. The ultrastructural findings consisted of a lining of mature squamous epithelium indicating the occurrence of metaplasia of the amniotic epithelium into the vaginal epithelium. The cells were arranged in layers as in the normal vaginal epithelium, i.e., superficial, intermediate and deep layers. There were desmosomes and cytoplasmic intermediate...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Amniotic membrane; Amnion; Electron microscopy; Congenital absence of vagina; Neovagina.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700007
Registros recuperados: 5
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