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A conglomeration of preclinical models related to myocardial infarction BJPS
Ahsan,Farogh; Mahmood,Tarique; Usmani,Shazia; Bagga,Paramdeep; Shamim,Arshiya; Tiwari,Reshu; Verma,Neeraj; Siddiqui,Mohammed Haris.
Cardiovascular diseases are the main source of death and morbidity in developed and developing nations. Animal models are required to propel our understanding of the pathogenesis, increase our knowledge, disease progress, and mechanism behind cardiovascular disorder, providing new approaches focused to improve the diagnostic and the treatment of these pathological conditions and additionally to test various therapeutic ways to deal with tissue regeneration and re-establish heart working following damage. A perfect model framework ought to be reasonable, effectively controlled, reproducible, and physiologically illustrative of human disease, show cardinal signs and pathology that resembles after the human ailment and ethically stable. The decision of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Myocardial infarction; Animal model; Isoproterenol; Chemical induced; Ischeamia.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100557
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An experimental model of mycobacterial infection under corneal flaps BJMBR
Adan,C.B.D.; Sato,E.H.; Sousa,L.B.; Oliveira,R.S.; Leão,S.C.; Freitas,D..
In order to develop a new experimental animal model of infection with Mycobacterium chelonae in keratomileusis, we conducted a double-blind prospective study on 24 adult male New Zealand rabbits. One eye of each rabbit was submitted to automatic lamellar keratotomy with the automatic corneal shaper under general anesthesia. Eyes were immunosuppressed by a single local injection of methyl prednisolone. Twelve animals were inoculated into the keratomileusis interface with 1 µl of 10(6) heat-inactivated bacteria (heat-inactivated inoculum controls) and 12 with 1 µl of 10(6) live bacteria. Trimethoprim drops (0.1%, w/v) were used as prophylaxis for the surgical procedure every 4 h (50 µl, qid). Animals were examined by 2 observers under a slit lamp on the 1st,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Infectious eye diseases; Keratitis; Animal model; Mycobacterium chelonae; Lamellar keratotomy.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700010
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Anatomical Characteristics of Deer and Sheep Lumbar Spines: Comparison to the Human Lumbar Spine International Journal of Morphology
Wang,Yang; Liu,Ting; Song,Liang-Song; Zhang,Zhi-Xin; Li,You-Qiong; Lu,Lai-Jin.
Deer and sheep spines are often used as models of the human spine. A prerequisite for the use of animal models is information regarding the interspecies differences in the parameters of general interest. This would clarify the limitations of each animal model and substantiate the applicability of the obtained results to humans. Since sufficient data appear to be currently unavailable, we sought to investigate the feasibility of using deer and sheep as animal models for studies on the human spine. The objective of this study was a thorough comparison of the anatomical parameters of deer and sheep spines with those of the human spine. We employed three-dimensional reconstructions of computed tomography images, generated using figure analysis software, which...
Tipo: Journal article Palavras-chave: Spine; Animal model; Comparative anatomy; Sheep; Deer; Human.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100018
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Anti-CD3 Antibody Ameliorates Transfusion-Associated Graft-Versus-Host Disease in a Chemotherapy-Based Mouse Model With Busulfan and Fludarabine BABT
Li,Xiaofan; Song,Qingxiao; Hu1,Wanyu; Wan,Bo; Huang,Qinghua; Li,Qing.
ABSTRACT To establish a transfusion-associated graft-versus-host disease (TA-GVHD) mouse model with busulfan and fludarabine for effective treatment evaluation. BALB/c (H-2d) mice were injected with busulfan (15 mg/kg) and fludarabine (30 mg/kg) twice a day for 4 days. The mice were transfused with 106 T cell-depleted bone marrow (TCD-BM )and cells in different groups 3 days after chemotherapy: syngeneic BALB/c, MHC minor mismatch DBA/2 (H-2d), or MHC major mismatch C57BL/6(H2-b). Recipient BALB/c mice were injected with either blood only or blood+splenocyte. TA-GVHD was monitored in terms of body weight loss, clinical scores, and survival. Dexamethasone (50 mg/kg), cyclophosphamide (50 mg/kg), cyclosporine A (30 mg/kg), and anti-CD3 (1 mg/kg) were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transfusion-associated graft-versus-host disease; Fludarabine; Busulfan; Animal model; Major histocompatibility complex.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100305
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Anxiolytic effect of methylene blue microinjected into the dorsal periaqueductal gray matter BJMBR
de-Oliveira,R.W.; Guimarães,F.S..
The dorsal periaqueductal gray (DPAG) has been implicated in the behavioral and autonomic expression of defensive reactions. Several results suggest that, along with GABA, glutamate and serotonin, nitric oxide (NO) may play a role in defense reactions mediated by this region. To further investigate this possibility we microinjected methylene blue (MB; 10, 30 or 100 nmol/0.5 µl) into the DPAG of rats submitted to the elevated plus-maze test, an animal model of anxiety. MB has been used as an inhibitor of soluble guanylate cyclase (sGC) to demonstrate cGMP-mediated processes, and there is evidence that NO may exert its biological effects by binding to the heme part of guanylate cyclase, causing an increase in cGMP levels. The results showed that MB (30 nmol)...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Anxiety; Elevated plus-maze; Nitric oxide; Animal model.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200012
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Avaliação de modelos uni e bicaracterística na estimativa de parâmetros genéticos de características leiteiras de cabras Saanen. Repositório Alice
ALVES, A. A. C.; PINZON, A. C.; LOBO, A. M. B. O.; FACO, O.; LOBO, R. N. B..
Resumo: Foram utilizadas 643 lactações de 261 cabras da raça Saanen para avaliar a eficiência dos modelos unicaracterística e bicaracterística em estimar parâmetros genéticos para as características produção de leite até 305 dias (PL305) e duração da lactação (DL). Os componentes de variância, bem como os parâmetros genéticos foram estimados pelo método da Máxima Verossimilhança Restrita Livre de Derivadas (DFREML) sob modelo animal. A matriz de parentesco continha 1.548 animais. As estimativas dos parâmetros genéticos para as características estudadas foram similares para os modelos avaliados. Entretanto, o modelo bicaracterística permitiu melhor utilização das informações disponíveis acerca das características estudadas. [Evaluation of single and...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Raça Saanen; Caprino; Cabra leiteira; Melhoramento genético animal; Goats; Animal model; Genetic correlation; Heritability; Milk yield.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1034779
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Avaliação do efeito materno sobre o peso aos 196 dias de idade em ovinos Santa Inês. Repositório Alice
SARMENTO, J. L. R.; SANTOS, G. V. dos; BIAGIOTTI, D.; SOUSA, W. H. de; LOBO, R. N. B..
Resumo: O trabalho teve como objetivo investigar a importância do efeito materno na estimação dos componentes de (co)variância e parâmetros genéticos sobre o peso aos 196 dias de idade (P196) em ovinos Santa Inês. Foi utilizado modelo animal que, além do efeito genético aditivo direto, incluiu ou não os efeitos aleatórios genético aditivo materno, covariância genética entre os efeitos direto e materno, ambiente permanente materno e o efeito comum de ninhada, mais o erro aleatório associado a cada observação. Com base no BIC o modelo que melhor se ajustou à característica foi que continha os efeitos genético aditivo e comum de ninhada (modelo 4), indicando que a não inclusão do efeito de ninhada em estudos genéticos para P196 pode levar a estimativas de...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Modelo animal; Componentes de variancia; Efeito materno; Ovino; Genética animal; Parâmetro genético; Melhoramento genético animal; Peso; Sheep; Animal breeding; Animal model; Genetic parameters; Variance components.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/939226
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Comparison of subcutaneous inflammatory response to commercial and engineered zinc hydroxyapatite implants in rabbits Arq. Bras. Med. Vet. Zootec.
Muñoz,L.C.; Cardona.-Ramirez,S.; Silva,R.F..
ABSTRACT Hydroxyapatite (HA) is widely used as a biomaterial for bone repair and metallic prostheses coating. The main limitations of the current commercial synthetic hydroxyapatite compounds include high cost and decreased availability, especially for veterinary medicine purposes. Additionally, it is thought that HA biocompatibility and bioactivity could be enhanced by the addition of metal compounds. The objective of this work was to compare the subcutaneous tissue response of commercial and engineered hydroxyapatite obtained from the bovine femur diaphysis mixed with different concentrations of hexa-hydrated Zinc Nitrate in rabbits. Twenty-Five New Zealand female rabbits were used. Five treatments were done according to HA composition (commercial HA, no...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal model; Biocompatibility; Bone; Integration.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601873
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Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis. Repositório Alice
ROSSONI, R. D.; BARBOSA, J. O.; VILELA, S. F. G.; SANTOS, J. D. dos; BARROS, P. P. de; PRATA, M. C. de A.; ANBINDER, A. L.; FUCHS, B. B.; JORGE, A. O. C.; MYLONAKIS, E.; JUNQUEIRA, J. C..
In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Galleria mellonella; Infection; Animal model.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035034
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COX-2 gene expression and methylation profile in Sapajus apella as an experimental model for gastric adenocarcinoma Genet. Mol. Biol.
Rosário Pinheiro,Danilo do; Harada,Maria Lucia; Rodriguez Burbano,Rommel Mario; Nascimento Borges,Barbara do.
Abstract Gastric cancer (GC) remains one of the main causes of cancer-related death worldwide. There are two distinct histological types of GC: diffuse and intestinal. The latter is characterized by the presence of pre-neoplastic lesions. One of the most frequently altered enzymes in intestinal GC is COX-2, an important lesion marker. This work aimed to study COX-2 methylation and expression in N-methyl-N-Nitrosurea (MNU)-induced intestinal GC in six Sapajus apella animals. The partial promoter sequence of S. apella COX-2 gene was obtained and used to identify transcription factors and cis-regulatory element binding sites. The COX-2 methylation pattern was assessed using Methylation-Specific PCR (MSP), and expression was analyzed by immunohistochemistry...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PTGS2; Gene regulation; Animal model; Gastric cancer.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300496
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Dietary models for inducing hypercholesterolemia in rats BABT
Matos,Sheyla Leite; Paula,Heberth de; Pedrosa,Maria Lúcia; Santos,Rinaldo Cardoso dos; Oliveira,Eduardo Luiz de; Chianca Júnior,Deoclécio Alves; Silva,Marcelo Eustáquio.
The present work aimed at finding a dietetical model capable of promoting the highest hypercholesterolemia without affecting the development of the rats. Sixty female Fisher rats were divided into five groups. The first one was fed a control diet; the remaining four were fed hypercholesterolemic diets with cholesterol and different contents of soybean oil, starch, casein, micronutrients and fiber and, consequently, different caloric values. After eight weeks animals were evaluated in relation to growth, fecal excretion, liver weight and fat, cholesterol and its fractions, serum biochemical parameters and sistolic pressure and compared with controls. The best result was obtained with the diet containing 25 % soybean oil, 1.0 % cholesterol, 13 % fiber and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hypercholesterolemia; Animal model; Caloric value; Cholesterol.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200006
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Efeito de linhagem citoplasmática em características de produção de ovinos da raça Merino. Repositório Alice
MORAIS, O. R de; BERGMANN, J. A. G.; OJEDA, D. B.; FONSECA, C. G. da; BARBOSA, P. F..
Objetivou-se avaliar a importancia dos efeitos de linhagem citoplasmatica sobre as caracteristicas peso corporal (PCORP), peso de velo sujo (PVS) e diametro da fibra da la (DIAM), respectivamente em 707 e 703 femeas e 350 machos da raca Merino com um ano de idade. Linhagens citoplasmaticas (LCT) foram definidas como as primeiras 41 femeas do pedigree registradas no Brasil, na decada de 30. Informacoes de pedigree incluiram 30640 animais e, em media, 10 geracoes. As avaliacoes foram feitas usando-se metolologia da Maxima Verossimillanca Restrita, sob modelo animal. Para cada caracteristica foram feitas analises com tres modelos (modelo com efeito direto; modelo com efeitos direto e de LCT; e modelo com efeitos direto, materno e de LCT). Os modelos...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Heranca citoplasmatica; Heranca mitocondrial; Linhagem materna; Ovino; Animal model; Cytoplasmic inheritance; Maternal lineage; Mitochondrial inheritance; Sheep.
Ano: 1998 URL: http://www.alice.cnptia.embrapa.br/handle/doc/44178
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Efeito materno sobre a curva de crescimento de ovinos Santa Inês por meio de modelos de regressão aleatória. Repositório Alice
SAMENTO, J. L. R.; TORRES, R. de A.; SOUSA, W. H. de; LOBO, R. N. B.; SOUSA, J. E. R. de; CAVALCANTE NETO, A.; DO Ó, A. O..
Utilizaram-se 17.767 registros de pesos de 4.210 cordeiros da raça Santa Inês com o objetivo de avaliar a importância da inclusão do efeito materno nos modelos de estimação de componentes de (Co) variância e parâmetros genéticos resultantes para a curva de crescimento, por meio de modelos de regressão aleatória. As regressões fixas e aleatórias foram ajustadas por meio de polinômios de Legendre de ordem três, testando-se quatro modelos que diferiram quanto à inclusão dos efeitos genético aditivo materno e ambiente permanente materno. Observou-se considerável aumento no Log L e diminuição nos critérios AIC e BIC quando se adicionou o efeito materno (genético ou ambiente permanente), evidenciando sua importância. O efeito genético materno explicou maior...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Polinômios de Legendre; Ovino; Raça Santa Inês; Genética animal; Herdabilidade; Modelo animal; Curva de crescimento; Sheep; Legendre polynomial; Animal genetic; Heritability; Animal model.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/902150
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Efeitos da linhagem citoplasmática sobre características de crescimento em bovinos da raça Canchim. Repositório Alice
ALENCAR, M. M. de; TREMATORE, R. L.; BARBOSA, P. F.; FREITAS, A. F. de..
O objetivo deste trabalho foi avaliar efeitos da linhagem citoplasmática (LC) sobre os pesos ao nascimento (PN), a desmama (PD, 240 dias) e aos 12 meses de idade (P12) de, respecitvamente , 4722, 3852 e 3389 bezerros da raça Canchim, provinientes de acasalamento de touros e vacas 5/8 Charolês + 3/8 Zebu e Canchim.Os animais 5/8 Charolês + 3/8 Zebu foram obtidos do cruzamento alternado entre raças Charolês x Indubrasil.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Gado de corte; Gado Charolês; Gado Indubrasil; Herança citoplasmática; Modelo animal; Pesos; Beef cattle; Cytoplasmatic iheritance; Animal model; Weights.
Ano: 1998 URL: http://www.alice.cnptia.embrapa.br/handle/doc/44175
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Effect of methylprednisolone on perivascular pulmonary edema, inflammatory infiltrate, VEGF and TGF-beta immunoexpression in the remaining lungs of rats after left pneumonectomy BJMBR
Guimarães-Fernandes,F.; Samano,M.N.; Vieira,R.P.; Carvalho,C.R.; Pazetti,R.; Moreira,L.F.P.; Pêgo-Fernandes,P.M.; Jatene,F.B..
Pneumonectomy is associated with high rates of morbimortality, with postpneumonectomy pulmonary edema being one of the leading causes. An intrinsic inflammatory process following the operation has been considered in its physiopathology. The use of corticosteroids is related to prevention of this edema, but no experimental data are available to support this hypothesis. We evaluated the effect of methylprednisolone on the remaining lungs of rats submitted to left pneumonectomy concerning edema and inflammatory markers. Forty male Wistar rats weighing 300 g underwent left pneumonectomy and were randomized to receive corticosteroids or not. Methylprednisolone at a dose of 10 mg/kg was given before the surgery. After recovery, the animals were sacrificed at 48...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pneumonectomy; Glucocorticoids; Pulmonary edema; Vascular endothelial growth factor; Transforming growth factor beta; Animal model.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700006
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Effects of bovine enterovirus and type 1 diabetes on liver and kidney pyruvate kinase activity in an animal model Biological Sciences
Dalzochio, Thaís; Nienov, Otto Henrique; Wannmacher, Clovis; Berlese, Daiane Bolzan; Feksa, Luciane Rosa.
Type 1 diabetes (T1D) is an autoimmune disease characterized by the selective destruction of pancreatic beta cells. In addition to genetic factors, enteroviruses have been considered the main environmental factor involved in this pathology. Therefore, the objective of this study was to evaluate the effects of streptozotocin-induced diabetes and bovine enterovirus (BEV) on liver and kidney pyruvate kinase activity in rats. Fourteen male Wistar rats were divided in three groups: control, diabetes and a third group, which was fed with water experimentally contaminated by BEV. Increased blood glucose levels were found in both diabetes and enterovirus groups, whereas there were no alterations in the lipid profile. A reduced pyruvate kinase activity was observed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal model; Autoimmune disease; Enzymatic activity..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/43765
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Efficient retrovirus-mediated transfer of cell-cycle control genes to transformed cells BJMBR
Strauss,B.E.; Costanzi-Strauss,E..
The use of gene therapy continues to be a promising, yet elusive, alternative for the treatment of cancer. The origins of cancer must be well understood so that the therapeutic gene can be chosen with the highest chance of successful tumor regression. The gene delivery system must be tailored for optimum transfer of the therapeutic gene to the target tissue. In order to accomplish this, we study models of G1 cell-cycle control in both normal and transformed cells in order to understand the reasons for uncontrolled cellular proliferation. We then use this information to choose the gene to be delivered to the cells. We have chosen to study p16, p21, p53 and pRb gene transfer using the pCL-retrovirus. Described here are some general concepts and specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene therapy; Clinical trial; Retrovirus; Animal model; Cell cycle; Growth arrest.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700016
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Elevated mazes as animal models of anxiety: effects of serotonergic agents Anais da ABC (AABC)
Pinheiro,Simone H.; Zangrossi-Jr.,Hélio; Del-Ben,Cristina M.; Graeff,Frederico G..
This article reviews reported results about the effects of drugs that act upon the serotonergic neurotransmission measured in three elevated mazes that are animal models of anxiety. A bibliographic search has been performed in MEDLINE using different combinations of the key words X-maze, plus-maze, T-maze, serotonin and 5-HT, present in the title and/or the abstract, with no time limit. From the obtained abstracts, several publications were excluded on the basis of the following criteria: review articles that did not report original results, species other than the rat, intracerebral drug administration alone, genetically manipulated rats, and animals having any kind of experimental pathology. The reported results indicate that the effect of drugs on the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal model; Elevated maze; Anxiety; Panic; Serotonin.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100010
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Establishment of a rat model for uterine leiomyomas based on Western and traditional Chinese medicine theories BJMBR
Zhao,Hui; Li,Yao; Xu,QiuXia; Peng,Fu; Zhao,JinShuang; Webb,R. Clinton; Peng,Cheng; Yu,ChengHao.
Uterine leiomyomas (ULs) are benign monoclonal tumors that arise from the underlying myometrial tissue in the uterus. Effective therapies are still lacking because of poor understanding of the pathophysiology and epidemiology. Hence, it is urgent to establish efficient animal models to screen novel anti-UL therapies. In this study, for the first time, traditional Chinese medicine and Western medicine were combined to establish an animal model of ULs in rats. In order to evaluate the function and value of the novel model, it was compared with other models. The long-term and short-term rat models for ULs were established using progesterone and diethylstilbestrol. Rats in Qi stagnation and blood stasis group were injected with epinephrine hydrochloride and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Uterine leiomyomas; Animal model; Traditional Chinese medicine.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900603
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Evaluation of a rat model of exercise-induced fatigue using treadmill running with progressively increasing load Anais da ABC (AABC)
HOU,LIJUAN; LI,KE; HU,YANRU; BIAN,YIJUN; JI,WEI; SHI,KAIXUAN; LI,YITING; CHEN,MENGJIAO; LI,JIAXIN; LIU,XIAOLI; QIAO,DECAI.
Abstract: The mechanism behind exercise-induced fatigue is a significant topic in the field of sports physiology. Therefore, establishing and evaluating an acute exercise-induced fatigue animal model that explores the limits of the motor system may provide greater insight into these mechanisms. Heart rate is an important quantitative parameter that accurately reflects the immediate change in physical function due to exercise load. And there is likely to be an important correlation between heart rate and behavioral performance. In this study, changes in heart rate and behavioral indexes during exercise-induced fatigue were quantitatively analyzed in rats using heart rate telemetry and video methods respectively. The behavioral indexes were used as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Exercise-induced fatigue; Animal model; Treadmill running; Heart rate.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700624
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