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A STUDY OF ASSOCIATION BETWEEN ELISA RESPONSE TO INFECTIOUS DISEASE AGENTS AND CALVING INTERVAL IN CATTLE IN THE TROPICS OF MÉXICO Ciência Rural
Barajas-Rojas,José Alfonso; Riemann,Hans; Franti,Charles.
Forty eight cows of Holstein-Zebu crosses with at least one completed pregnancy were tested for antibodies to 20 disease agents. The testing was performed within one month (average two weeks) after calving when the IgG level constantly reaches its lowest value during the cows reproductive cycle. The results indicate that increasing levels of IgG against Leptospira interrogans serovar hardjo and Mycobacterium paratuberculosis may be associated with an increased calving interval. Increase in calving intervals could not be explained by age of cows or their genotypic characteristics.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Infectious diseases; Antibodies; Calving intervals; México.
Ano: 1993 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000300015
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Accuracy of immunoglobulin M and immunoglobulin A of saliva in early diagnosis of dengue: Systematic Review and Meta-analysis Anais da ABC (AABC)
COLONETTI,TAMY; ROCHA,BELISE V.E.; GRANDE,ANTÔNIO J.; ALEXANDRE,MARIA C.M.; DONDOSSOLA,EDUARDO R.; MADEIRA,KRISTIAN; ROSA,MARIA I..
ABSTRACT The objective of the study was to conduct a systematic review to synthesize the current evidence on the accuracy of IgM and IgA to early diagnosis the dengue virus. The review protocol was registered at PROSPERO (CRD 42015024808). We searched for studies in the following electronic database from 1990 to January 2018. The search identified 3507 studies. Five studies were included for quantitative analysis. Three studies included evaluations of salivary IgM provided a sensitivity of 86% and specificity of 93%. Two studies included evaluating of IgA salivary showed a combined sensitivity of 69% and a combined specificity of 98%. Despite the results found and the low methodological quality of the studies included in the meta-analysis it is still soon...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunoglobulin M; Immunoglobulin A; Antibodies; Dengue virus; Diagnosis..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000603147
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Antibodies against pseudorabies virus in feral swine in southeast Brazil Arq. Bras. Med. Vet. Zootec.
Cunha,E.M.S.; Nassar,A.F.C.; Lara,M.C.C.S.H.; Bersano,J.G.; Villalobos,E.M.C.; Oliveira,J.C.F..
Serum samples collected from 358 wild boars (Sus scrofa) in breeding farms in São Paulo, southeast Brazil, from 1998 to 2001, were tested for antibodies against pseudorabies virus (PRV) by means of serum neutralization (SN) and enzyme-linked immunobsorbent assay (ELISA). Seropositive animals were detected in three of seven herds analyzed. Overall seroprevalence as assessed by SN was 30.7%, ranging from 25.2% to 100% for the herds that presented seropositive animals. Indirect ELISA detected lower seroprevalence (19.3%). Sensitivity and specificity of ELISA were equal to 57.3% and 97.6%, respectively. Agreement was equal to 85.2% (P<0.0001). These results showed that PRV infections occurred in farmed feral swine in southeast Brazil, and affect...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wild boar; Sus scrofa; Pseudorabies virus; Aujeszky’s disease virus; Antibodies.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400002
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Anti-influenza A virus antibodies in Tayassuidae from commercial rearing farms in Brazil Ciência Rural
Baraldi,Thaís Gasparini; Almeida,Henrique Meiroz de Souza; Morais,Amanda Bonalume Cordeiro de; Storino,Gabriel Yuri; Montassier,Hélio José; Motta,Rodrigo Garcia; Ribeiro,Márcio Garcia; Oliveira,Luís Guilherme de.
ABSTRACT: Family Tayassuidae in the suborder Suina include two species of peccaries in Brazil: the white-lipped peccary (Tayassu pecari) and the collared peccary (Pecari tajacu). These animals share common pathogens with domestic swine (Sus scrofa); however, their role as potential carrier remains unclear. This study focused on detecting the prevalence of influenza A antibodies in Tayassu pecari and Pecari tajacu from commercial rearing farms from two states in Brazil. A set of 50 blood samples from Pecari tajacu and 55 from Tayassu pecari were analyzed using a commercial indirect ELISA in order to investigate anti influenza A antibodies. Pecari tajacu samples presented 22% (11/50) of seropositivity for the virus. Serological surveillance is an important...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Influenza; Antibodies; Peccaries.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000400451
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Anti-leishmania antibodies in cerebrospinal fluid from dogs with visceral leishmaniasis BJMBR
Lima,V.M.F.; Gonçalves,M.E.; Ikeda,F.A.; Luvizotto,M.C.R.; Feitosa,M.M..
Visceral leishmaniasis in Brazil is caused by Leishmania (Leishmania) chagasi and the dog is its most important reservoir. The clinical features in dogs include loss of weight, lymphadenopathy, renal failure, skin lesions, fever, hypergammaglobulinemia, hepatosplenomegaly, anemia, and, rarely, neurological symptoms. Most infected animals develop active disease, characterized by high anti-leishmania antibody titers and depressed lymphoproliferative ability. Antibody production is not primarily important for protection but might be involved in the pathogenesis of tissue lesions. An ELISA test was used to determine if there is an association between neurological symptoms and the presence of anti-L. chagasi antibodies in cerebrospinal fluid (CSF). Thirty serum...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Leishmania (Leishmania) chagasi; Visceral leishmaniasis; Cerebrospinal fluid; Dogs; Antibodies.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400010
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Are anti-interferon antibodies the cause of failure in: chronic HCV hepatitis treatment? BJID
Barone,Antonio Alci; Tosta,Rose Aparecida Borges; Tengan,Fátima Mitiko; Marins,José Humberto Caetano; Cavalheiro,Norma de Paula; Cardi,Bruno Andrade.
A follow-up study was made of 94 chronic hepatitis C patients at a hepatitis clinic in Brazil, after interferon alpha (IFN-alpha) therapy, to determine the influence of anti-interferon antibodies on treatment outcome. Patients diagnosed as having chronic hepatitis C, confirmed by PCR (HCV RNA) and liver biopsy, were treated with interferon alpha 2a or 2b for at least six months, and were followed up for 24 weeks after termination of treatment in order to assess biochemical, virological and clinical pathology responses. Only 6% of the 94 patients developed anti-IFN antibodies, 70% presented a biochemical response and 23% maintained a sustained virological response. Clinical evaluation revealed that in only 2 patients was there progression of fibrosis; the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Interferon-alpha; Antibodies; Chronic HCV; Treatment.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100002
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Assessment of the plan for pre-exposition vaccination with Fuenzalida-Palacios anti-rabies vaccine BABT
Preto,Angela Aparecida; Gomes,Elisa Maines; Fernandes,Maurício José; Hennig,Nei Adolfo; Germano,Pedro Manoel Leal.
Studies ere carried out to monitor the titres of anti-rabies antibodies taken from individuals exposed to the risk of infection. Fifty-seven individuals aged between 20 and 40, were vaccinated with a minimum titre of 1.3 IU Fuenzalida-Palacios type anti-rabies vaccine. The vaccination plan consisted of the application of three doses at two day intervals and a booster applied 28 days after the final dose. The accompainment of the antibody titres was achieved by means of the seroneutralization test over a total period of 18 months. Through the monitoring carried out, it was found to be necessary to apply a booster vaccination to part of the group. Some of these individuals were revaccinated with Fuenzalida-Palacios type vaccine with a minimum titre of 1.3...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunization; Rabies vaccine; Antibodies; Neutralization; Fuenzalida-Palacios.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300002
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Avaliação de níveis séricos de imunoglobulina, proteína e o desempenho de bezerras da raça Holandesa. Repositório Alice
PAULETTI, P.; MACHADO NETO, R.; PACKER, I.U.; BESSI, R..
Os níveis séricos de imunoglobulina G, proteína total e o desempenho foram avaliados em 59 bezerras da raça Holandesa do nascimento até 60 dias de idade, em um delineamento experimental inteiramente casualizado em parcelas subdivididas no tempo. Os animais foram separados de acordo com a concentração inicial de imunoglobulinas séricas adquiridas passivamente e alocados nos seguintes grupos: grupo 1: animais com baixo nível de imunidade passiva (até 20 mg/mL de IgG); grupo 2: animais com nível médio de imunidade passiva (entre 20 a 30 mg/mL de IgG), e grupo 3: animais com alto nível de imunidade passiva (acima de 30 mg/mL de IgG). Picos de concentrações de proteína total em todos os grupos experimentais foram encontrados nos primeiros dias de vida,...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Anticorpos; Colostro; Imunidade passiva; Anabolismo; Catabolismo; Antibodies; Colostrum; Passive immunity; Anabolism; Catabolism.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/106117
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Colostral immunoglobulins absorption in Canchim and Nelore calves R. Bras. Zootec.
Machado Neto,Raul; Packer,Irineu Umberto; Prado,Gladys Villas Boas do; Bessi,Rosana; Pauletti,Patricia.
The efficiency of absorption of colostral immunoglobulins was evaluated in five Canchim and seven Nelore calves. They received colostrum pools with concentration of 70.20 ± 6.14 mg/mL through esofageal feeder at 2, 12, 24 and 36 hours after birth. The immunoglobulins concentrations of the pools were estimated through specific gravity and measured by radial immunodifusion. In the blood collection at birth and during the first 70 days of life, the total protein was assayed by biuret method and the immunoglobulins were assayed by radial immunodifusion. Data were analysed as a randomized split-plot statistical model. The highest concentrations of serum immunoglobulins and total protein were observed at 24 hours of age. No significant differences (P>0.5484)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibodies; Colostrum; Newborn calves; Passive immunity.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982004000600021
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Comparação dos antígenos CAE/OPP e CAEV utilizando o teste de imunodifusao em gel de agarose (IDGA). Repositório Alice
ALVES, F. S. F.; PINHEIRO, R. R.; PIRES, P. C..
1997
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: CAEV; Teste sorológico; Artrite-encefalite Caprina; IDGA; Técnica imunológica; Caprino; Artrite; Diagnóstico; Doença animal; Anticorpo; Vírus; Goats; Encephalitis; Animal diseases; Diagnostic; Antibodies; Caprine arthritis encephalit virus; Immunological techniques.
Ano: 1997 URL: http://www.alice.cnptia.embrapa.br/handle/doc/514850
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Comparison of dot-ELISA and standard ELISA for detection of Neisseria meningitidis outer membrane complex-specific antibodies BJID
Belo,Elza FT; Farhat,Calil K; Gaspari,Elizabeth N De.
Dot-ELISA using the outer membrane complex antigens of Neisseria meningitidis as a target was standardized for rapid detection of meningococcal-specific antibodies in human serum. We investigated the level of meningococcal-specific IgG, IgA, and IgM in serum using dot-ELISA with outer membrane antigens prepared from Neisseria meningitidis serotype B:4.19:P1.15,3,7,9 (a strain isolated from a Brazilian epidemic). The dot-ELISA is based on the same principles as the standard ELISA and is useful for detection of anti-N. meningitidis B antibodies in serum of patients with meningococcal infections. For the assay, outer membrane complexes (OMCs) were absorbed by nitrocellulose membrane and blocked with a 5% skim milk solution. Serum samples were drawn upon...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neisseria meningitidis; Dot-ELISA; ELISA; Antibodies; Outer membrane complex.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100008
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Desenvolvimento de uma prova de imunoadsorcao enzimática para detecção de anticorpos contra Trypanosoma vivax em bovinos: resultados preliminares. Infoteca-e
MADRUGA, C. R.; ARAUJO, F. R.; CRUZ, T. M. da; SCHENK, M. A. M..
1999
Tipo: Séries anteriores (INFOTECA-E) Palavras-chave: Bovino; Trypanosoma vivax; Anticorpo; ELISA; Mato Grosso; Mato Grosso do Sul; Brasil; Cattle; Antibodies; Brazil.
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/322104
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Detection of antibodies to Toxoplasma gondii in wild animals in Brazil J. Venom. Anim. Toxins incl. Trop. Dis.
Silva,Rodrigo Costa da; Machado,Gustavo Puglia; Cruvinel,Tatiane Morosini de Andrade; Cruvinel,Ciro Alexandre; Langoni,Helio.
Background Toxoplasmosis is a worldwide zoonosis caused by an obligate intracellular protozoan parasite,Toxoplasma gondii, that affects all warm-blooded animals, including wild animals. The increased number of cases of parasitic infections is mainly due to the destruction of environmental conservation areas, which is driving wild animals out of their habitats and towards urban areas. In this study, the occurrence of T. gondii infection was investigated by the modified agglutination test (MAT) in 26 different species of run over and injured wild animals that were treated at a Brazilian university veterinary hospital, from June 2007 to August 2008.Findings Of the studied animals, six (23.1%; CI95% 11.1-42.2%) had T. gondii antibodies, with titers equal to 10...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxoplasma gondii; Natural infection; Antibodies; Wild animals; MAT.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200603
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Development of an enzyme-linked immunosorbent assay for quantification of Salmonella enteritidis-specific antibodies in egg yolk Rev. Bras. Ciênc. Avic.
Tayeb,IT; Nehme,P; Jaber,L; Barbour,EK.
The present study aims at developing an indirect ELISA to quantify yolk antibodies specific to all surface proteins of the invasive Salmonella Enteritidis (SE), which acquired the 1.8, 14.1, and ~ 50 Kb plasmids. An ELISA checkerboard was used in four different experiments to account for the different parameters included in the preliminary ELISA procedure, and consequently to maximize the difference in Optical Density (OD) values between control positive and negative yolk samples. The first experiment aimed at studying the impact of 5% Bovine Serum Albumin (BSA) dissolved in distilled water as a blocking reagent on a 28 µg/well SE antigen-coated plate, while applying the positive and negative control yolk samples to different concentrations of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibodies; Egg yolk; ELISA; Salmonella Enteritidis.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400009
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Diagnosis of American cutaneous leishmaniasis by enzyme immunoassay in patients from Northern Paraná State, Brazil BJID
Yoneyama,Kelly Aparecida Geraldo; Peder,Leyde Daiane de; Lonardoni,Maria Valdrinez Campana; Silveira,Thaís Gomes Verzignassi.
American cutaneous leishmaniasis (ACL) is an endemic disease caused by Leishmania parasites. The ACL diagnosis is commonly accomplished by parasitological and immunological methods. The objective of this work was to evaluate the enzyme immunoassay (EIA) for ACL laboratorial diagnosis. IgG antibodies against Leishmania (Viannia) braziliensis promastigotes were researched. For the method standardization 240 sera were used: 72 from patients with positive parasitological diagnosis, 38 from normal individuals and 113 from individuals with other pathologies. The sensibility was 93% and the specificity was 70%. Regarding diagnosis, EIA showed 97% positivity in patients with ACL and 97% negativity in patients with other cutaneous lesions. The EIA presented a good...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutaneous leishmaniasis; Diagnosis; Antibodies; Immunoenzyme; Leishmania braziliensis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300012
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Diagnosis of infectious bronchitis: an overview of concepts and tools Rev. Bras. Ciênc. Avic.
Villarreal,LYB.
Infectious bronchitis (IB) casues multi-systemic infection in chickens with signs similar caused by other poultry pathogens and thus a high diagnostic accuracy can only be achieved by s series of laboratory assays. This article reviews in a brief way the traditional virus assays such as embryo innoculation, tracheal rings and virus neutralization assays for the direct detection of Avian infectious bronchitis virus (IBV) and methods based on gene molecular biology and some assays for the detection of anti-IBV antibodies, including ELISA. A critical view on each technique is also provived by the author.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibodies; Diagnosis; IBV; Typing.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200006
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DNA vaccines for viral diseases BJMBR
Donnelly,J.J.; Ulmer,J.B..
DNA plasmids encoding foreign proteins may be used as immunogens by direct intramuscular injection alone, or with various adjuvants and excipients, or by delivery of DNA-coated gold particles to the epidermis through biolistic immunization. Antibody, helper T lymphocyte, and cytotoxic T lymphocyte (CTL) responses have been induced in laboratory and domesticated animals by these methods. In a number of animal models, immune responses induced by DNA vaccination have been shown to be protective against challenge with various infectious agents. Immunization by injection of plasmids encoding foreign proteins has been used successfully as a research tool. This review summarizes the types of DNA vaccine vectors in common use, the immune responses and protective...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plasmid; Nucleic acid; Protection; Antibodies; T cells; CTL.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200010
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Duração da imunidade passiva para lentivírus de pequenos ruminantes em cordeiros. Repositório Alice
SOUZA, T. S. de; COSTA, J. N.; PINHEIRO, R. R.; MELO, F. C. C. de; LIMA, C. C. V. de; ANDRIOLI, A.; AZEVEDO, D. A. A. de; SANTOS, V. W. S. dos; OLIVEIRA, E. L. de; COSTA NETO, A. de O..
Resumo: Com a finalidade de avaliar a imunidade passiva contra lentivírus de pequenos ruminantes (LVPR), em cordeiros, este estudo foi conduzido a partir de dois grupos experimentais. O primeiro (G1) foi estabelecido por nove cordeiros submetidos à mamada artificial de pool de colostro de cabras positivas para LVPR. O segundo (G2) foi o controle, constituído por dez cordeiros submetidos à mamada natural de colostro das suas mães negativas. Amostras de sangue foram obtidas antes da primeira mamada, após 24h do nascimento e com sete, 15, 30, 50, 70, 90 e 120 dias de vida. Determinaram-se as concentrações de proteína sérica total (PST), albumina (ALB), globulinas (GLOB) e imunoglobulina G (IgG) e anticorpos anti-LVPR foram pesquisados a partir das técnicas de...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Anticorpos colostrais; Imunidade passiva; Lentivirose; Imunodiagnóstico; Ovino; Cordeiro; Colostro; Anticorpo; Sheep; Passive immunity; Lentivirus; Antibodies; Immunodiagnosis; Colostrum; ELISA; Diagnosis.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1000640
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Efeito da raça, dieta, época e ordem de parição na concentração de imunoglobulina G no colostro de suínos. Repositório Alice
MACHADO NETO, R.; PACKER, I.U.; MENTEN, J.F.; LAVORENTI, A..
Este trabalho teve por objetivo estudar os efeitos da raça, dieta, época do ano e ordem de parição na concentração de imunoglobulina G (IgG) no colostro de porcas. A concentração de IgG foi determinada no colostro de 60 porcas, 33 da raça Large White e 27 da raça Landrace, submetidas a dietas contendo 0%, 7%, 14% e 21% de levedura seca (LS). A levedura seca de destilaria de álcool de cana-de-açúcar (Saccharomyces cerevisiae) substituiu parte do milho e do farelo de soja da ração mantendo o nível de 14% de proteína bruta. Não foi verificado efeito significativo (P>0,05) de raça e da dieta sobre a concentração de IgG do colostro. Os valores mais elevados de IgG foram observados no colostro de porcas que pariram entre maio e outubro (P<0,05). Foram...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Imunidade passiva; Porcas; Anticorpos; Fatores imunológicos; Passive immunity; Sows; Antibodies; Immunological factors.
Ano: 2001 URL: http://www.alice.cnptia.embrapa.br/handle/doc/83521
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Effect of bee pollen on the immunity and tibia characteristics in broilers Rev. Bras. Ciênc. Avic.
Oliveira,MC de; Silva,DM da; Loch,FC; Martins,PC; Dias,DMB; Simon,GA.
This study was carried out to evaluate the effect of bee pollen (BP) levels on the IgG and IgM titers, weight of lymphoid organs, and on the tibia morphometric measures and mineralization in broilers at 21 and 42 days of age. Four hundred birds were used in an entirely randomized design with four treatments (0, 0.5, 1 and 1.5% of BP feed inclusion) and five replicates. At 21 and 42 days of rearing, blood samples were collected for IgG and IgM analysis, as well as lymphoid organs (bursa, thymus and spleen) and the tibiae. There was no effect (p>0.05) of the BP inclusion on IgG titers, bursa and spleen weights, tibia morphometric measures and mineralization at 21 and 42 days, IgM titer at 42 days or thymus weight at 21 days. However, IgM titers at 21 days...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibodies; Bee products; Lymphoid organs.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000400006
Registros recuperados: 58
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