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Aging reduces the primary humoral response and the in vitro cytokine production in mice BJMBR
Simioni,P.U.; Costa,E.H.; Tamashiro,W.M.S.C..
Aging is accompanied by a decrease in several physiological functions that make older individuals less responsive to environmental challenges. In the present study, we analyzed the immune response of female BALB/c mice (N = 6) of different ages (from 2 to 96 weeks) and identified significant age-related alterations. Immunization with hapten-protein (trinitrophenyl-bovine serum albumin) conjugates resulted in lower antibody levels in the primary and secondary responses of old mice (72 weeks old). Moreover, young mice (2, 16, and 32 weeks old) maintained specific antibodies in their sera for longer periods after primary immunization than did old mice. However, a secondary challenge efficiently induced memory in old mice, as shown by the increased antibody...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aging; Cytokines; Antibody; Proliferation; T cell response; T cell subsets.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800012
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Antibodies specific to infectious bronchitis in broilers in Ceará state, Brazil Arq. Bras. Med. Vet. Zootec.
Cardoso,W.M.; Gomes,L.P.; Romão,J.M.; Salles,R.P.R.; Teixeira,R.S.C.; Sobral,M.H.N.R.; Câmara,S.R.; Oliveira,W.F..
The experiment was carried out to determine the antibody levels to infectious bronchitis virus (IBV) in 1120 broilers of two broiler flocks, both from the same parental flock and free from previous vaccination. Forty chicks of each line were alloted to the control group and the sera were tested by indirect ELISA. The vaccination program consisted on the administration of commercial vaccines against IBV at 10 and 25 days of age. Chicks with low levels of maternal antibodies (Mab) did not show significant titers to the first vaccinal stimulus. They presented a vaccinal response to the second vaccinal stimulus reaching the top around GMT 1100 at 45 days. Chicks with high Mab titers did not show significant titers to the primary and secondary vaccinal stimuli,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler; Infections bronchitis; Antibody.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300007
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Antibody conjugate radioimmunotherapy of superficial bladder cancer BABT
Perkins,Alan; Hopper,Melanie; Murray,Andrea; Frier,Malcolm; Bishop,Mike.
The administration of antibody conjugates for cancer therapy is now proving to be of clinical value. We are currently undertaking a programme of clinical studies using the monoclonal antibody C595 (IgG3) which reacts with the MUC1 glycoprotein antigen that is aberrantly expressed in a high proportion of bladder tumours. Radioimmunoconjugates of the C595 antibody have been produced with high radiolabelling efficiency and immunoreactivity using Tc-99m and In-111 for diagnostic imaging, and disease staging and the cytotoxic radionuclides Cu-67 and Re-188 for therapy of superficial bladder cancer. A Phase I/II therapeutic trail involving the intravesical administration of antibody directly into the bladder has now begun.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibody; Radioimmunotherapy; Bladder cancer.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500012
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Antigenic Relationship among Kinetoplastid Flagellates : Herpetomonas, Leishmania and Bodo OAK
De, A.; Das, Pradeep; Bhattacharya, Amal; Das, D..
Palavras-chave: Antigen; Antibody; Immunoreactivity; Kinetoplastid flagellates.
Ano: 1994 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/215
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Assessment of molecular recognition element for the quantification of human epidermal growth factor using surface plasmon resonance Electron. J. Biotechnol.
Rosti,Ira Amira; Ramanan,Ramakrishnan Nagasundara; Ling,Tau Chuan; Ariff,Arbakariya B.
Background: A method for the selection of suitable molecular recognition element (MRE) for the quantification of human epidermal growth factor (hEGF) using surface plasmon resonance (SPR) is presented. Two types of hEGF antibody, monoclonal and polyclonal, were immobilized on the surface of chip and validated for its characteristics and performance in the quantification of hEGF. Validation of this analytical procedure was to demonstrate the stability and suitability of antibody for the quantification of target protein. Results: Specificity, accuracy and precision for all samples were within acceptable limit for both antibodies. The affinity and kinetic constant of antibodies-hEGF binding were evaluated using a 1:1 Langmuir interaction model. The model...
Tipo: Journal article Palavras-chave: Antibody; BIAcore; Biosensors; Epidermal growth factor; Validation.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600015
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Clinical and laboratory aspects of common variable immunodeficiency Anais da ABC (AABC)
Kokron,Cristina M.; Errante,Paolo R.; Barros,Myrthes T.; Baracho,Gisele V.; Camargo,Maristela M.; Kalil,Jorge; Rizzo,Luiz V..
Common variable immunodeficiency (CVID) is an immunological disorder characterized by defective antibody production, recurrent infections, most notably of the respiratory tract, autoimmune phenomena and cancer. Some CVID patients may also present disturbances of the cellular immune response such as a decrease in the number and proportion of different lymphocyte populations, diminished lymphoproliferative response to mitogens and antigens, altered production of cytokines, and deficient expression of cell-surface molecules. Most Brazilian CVID patients included in this study show a decrease in T and B lymphocyte counts in the peripheral blood. Furthermore, their lymphocytes are more susceptible to apoptosis following activation than normal individuals, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunodeficiency; Humans; Antibody; Cytokines; Clinical features.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000400007
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Concentração de IgG no colostro de éguas e soro sanguineo da cria. Repositório Alice
UNANIAM, M.M.; SILVA, A.E.D.F.; PEREIRA, A.C..
O colostro constitui fonte de nutrientes e imunoglobulinas fundamentais para a sobrevivência do recem-nascido.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Eqüinos; Antibioticos; Primeiro parto; Cria; Soro sanguineo; Equine; Antibody; IDR; Time of first suckling.
Ano: 1995 URL: http://www.alice.cnptia.embrapa.br/handle/doc/43482
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Detection of anti: Rickettsia spp. antibodies in domestic chickens of extensive breeding in an endemic area for spotted fever in the state of Rio Grande do Sul, Brazil Ciência Rural
Maciel,Jonas Fernandes; Krawczak,Felipe da Silva; Oliveira,Caroline Sobotyk de; Moraes-Filho,Jonas; Labruna,Marcelo Bahia; Botton,Sônia de Avila; Vogel,Fernanda Silveira Flores; Sangioni,Luis Antonio.
The goal of this study was to investigate anti-Rickettsia spp. antibodies in sera of domestic chickens (Gallus gallus domesticus) of extensive breeding in Cerro Largo county, considered an endemic area for spotted fever in the State of Rio Grande do Sul, Brazil. Three hundred blood samples were collected and anti-Rickettsia spp. antibodies were evaluated by indirect immunofluorescence assay (IFA) in the sera obtained. The occurrence of anti-Rickettsia spp. antibodies detected in this study was 1.33% (4/300), with endpoint titers ranging from 64 to 256 for Rickettsia rickettsiii, R. parkeri and/or R. bellii. The results suggest these domestic chickens do not participate as a reservoir and/or amplifying host in the epidemiology of spotted fever in that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Indirect immunofluorescence assay; Antibody; Rickettsiosis; Domestic chicken.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001100019
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Development of a caspase-3 antibody as a tool for detecting apoptosis in cells from rainbow trout (Oncorhynchus mykiss) Electron. J. Biotechnol.
Rojas,Verónica; Guzmán,Fanny; Valenzuela,Cristián; Marshall,Sergio H; Mercado,Luis.
Background: Apoptosis is an active cell death process mediated by caspases activation, in which different extrinsic or intrinsic signalling pathways result in direct activation of effector caspases. Caspase-3 is considered to be the most important of the executioner caspases, which cause the morphological and biochemical changes detected in apoptotic cells. Different bacterial and virus pathogens have developed different strategies to survive inside the host and overcome natural protections, one of them is inducing apoptotic death in infected cells. We have demonstrated previously that Piscirickettsia salmonis activates this process in monocytes/macrophages from salmonid RTS11 cell line both by morphological and caspase detection assays; nevertheless,...
Tipo: Journal article Palavras-chave: Antibody; Caspase-3; Fish apoptosis; Piscirickettsia salmonis.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500012
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Effect of aging and oral tolerance on dendritic cell function BJMBR
Simioni,P.U.; Fernandes,L.G.R.; Gabriel,D.L.; Tamashiro,W.M.S.C..
Oral tolerance can be induced in some mouse strains by gavage or spontaneous ingestion of dietary antigens. In the present study, we determined the influence of aging and oral tolerance on the secretion of co-stimulatory molecules by dendritic cells (DC), and on the ability of DC to induce proliferation and cytokine secretion by naive T cells from BALB/c and OVA transgenic (DO11.10) mice. We observed that oral tolerance could be induced in BALB/c mice (N = 5 in each group) of all ages (8, 20, 40, 60, and 80 weeks old), although a decline in specific antibody levels was observed in the sera of both tolerized and immunized mice with advancing age (40 to 80 weeks old). DC obtained from young, adult and middle-aged (8, 20, and 40 weeks old) tolerized mice were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aging; Oral tolerance; Dendritic cell; Antibody; Proliferation; Cytokine secretion.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100010
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Elisa evaluation of the levels of antibodies against Infectious Bronchitis Virus in laying hens using egg yolk as substrate Rev. Bras. Ciênc. Avic.
Rauber,RH; Flôres,ML; Pereira,CE; Grigulo,M.
The present work was carried out to compare Infectious Bronchitis Virus (IBV) antibody titers in serum and egg yolk samples from laying hens. Sixty paired blood and egg samples were collected from laying hens of two farms. Serum samples were frozen, while egg yolk samples were diluted (1:500) before freezing. Serum and yolk samples were tested for the presence of IBV antibodies by indirect ELISA (commercial kit) and titers were compared by a correlation test (alpha=0.05). There was a high correlation (r=0.62) between the two kinds of samples, which means that titers of IBV antibodies in the egg yolk and in serum samples are quite the same. Considering that blood collection causes deep stress that leads to economic losses, and since eggs are collected daily...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibody; Egg yolk; ELISA; Infectious bronchitis; Serology.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200008
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Epitopic Specificity of the Human Immune Response to the Invariant Region of a Polymorphic Plasmodium faciparum Merozoite Surface Antigen OAK
Rzepczyk, C. M.; Anderson, K. L.; Crurshes, P. A.; Baxter, E. P.; Kere, N.; Irving, D.; Dyer, S.; Jones, G. L..
Merozoite surface antigen 2 (MSA2) is a Plasmodium falciparum vaccine candidate. Antibody responses to MSA2 in Melanesians naturally exposed to P. falciparum were investigated by ELISA using the recombinant MSA2 protein known as Ag 1609 and 65 overlapping synthetic peptides spanning predominantly the conserved N and C terminal regions of MSA2. Significant differences were obtained between control and malaria exposed subjects in the ability of sera to bind to Ag 1609. The median absorbance obtained with control sera was 0.36 whereas that obtained with sera from malaria exposed subjects was 1.8. The sera of ninety-five percent of malaria exposed subjects reacted significantly with Ag 1609. The percentage reactivity of the control sera with Ag 1609 was 39%....
Palavras-chave: Malaria; Merozoite surface antigen 2 (MSA2); Antibody.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/170
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Expressão da neurotoxina TX4(6-1) em sistema heterólogo e bioensaio com a lagarta do cartucho (Spodoptera frugiperda). Repositório Alice
FIGUEIREDO, J. E. F.; ROCHA, T.L.; FONTES, M.A.; CRUZ, I.; KALAPOTHAKIS, E.; GOMEZ, M.V.; PURCINO, A. A. C..
2000
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Praga; Inseto; Lagarta do cartucho; Neurotoxina; Proteina; Fusao; Anticorpos; Controle biologico; Phoneutria nigriventes; Spodoptera frugiperda; Pest; Insecta; Protein; Antibody; Biological control; Fusion.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484013
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Expressão da neurotoxina TX4-(6-1) em sistema heterólogo e bioensaio com a lagarta-do-cartucho (Spodoptera frugiperda). Repositório Alice
FIGUEIREDO, J. E. F.; ROCHA, T.L.; FONTES, M.A.; CRUZ, I.; KALAPOTHAKIS, E.; GOMEZ, M.V.; PURCINO, A. A. C..
2000
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Praga; Inseto; Lagarta do cartucho; Neurotoxina; Proteina; Fusao; Anticorpos; Controle biologico; Phoneutria nigriventer; Spodoptera frugiperda; Pest; Insecta; Antibody; Biological control; Protein; Fusion.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/483854
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FISH IMMUNE SYSTEM Gayana
Olabuenaga,Susana E..
The defence against infections is the essential function of the immune system of all Vertebrates. Fish present an efficient and developed immunologic response and in the case of Teleosts, with some similarities to the higher vertebrates. It can be divided into two types: innate or non specific and adquired or specific, both with a humoral and cellular compartments. Their relative importance can change according to the age of fish and is always under the influence of different factors. The most relevant components are mentioned here. There are two factors in fishes which strongly influence the immune response and antibody production, seasonal changes and temperature. The main lymphoid organs are described here: the thymus, the kidney and the spleen, as well...
Tipo: Journal article Palavras-chave: Antigen; Antibody; Leukocytes; Phagocytosis; Lymphokines; Inflammation; Immunostimulants.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200010
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Homologous ELISA procedure for the determination of penaeid shrimp vitellogenin ArchiMer
Mendoza, Roberto; Guillaume, Jean-charles; Fauvel, Christian.
Vitellogenin (VTG) was isolated from the haemolymph of Penaeus vannamei by a three step procedure including ultracentrifugation, gel filtration and ion exchange chromatography. VTG was used to raise polyclonal antibodies that were purified by ion exchange chromatography. A two step competitive assay was developed in which VTG could be quantitated by its capacity to inhibit the binding of antibody to the VTG previously adsorbed onto a solid phase. Sensitivity from equilibrium and from non-equilibrium assays was 41 and 2.3 ng/ml respectively. Estimates of within-assay and between-assay variabilities of standard curves were 4.2 and 9.6% respectively. The inhibition curves for dilutions of haemolymph from vitellogenic females, egg yolk extracts and purified...
Tipo: Text Palavras-chave: Pénéidé; Vitellogénine; Vitelline; Immuno-essai (ELISA); Anticorps; Penaeid; Vitellogenin; Vitellin; Immunoassay (ELISA); Antibody.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00188/29894/28333.pdf
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Immobilization of Protein A on SAMS for the elaboration of immunosensors ArchiMer
Briand, Elisabeth; Salmain, Michèle; Compere, Chantal; Pradier, Claire-marie.
Binary mixtures of 11-mercaptoundecanoic acid (MUA) and other thiols of various lengths and terminal functions were chemisorbed on gold-coated surfaces via S-Au bonds to form mixed self-assembled monolayers (SAMs). Several values of the inole fraction of MUA in the thiol mixtures were tested and the structure and composition of the resulted thin films were characterized by X-ray photoelectron spectroscopy (XPS) and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). The results made it clear that co-adsorption of MUA with thiols of similar chain length led to well-ordered monolayers whereas the co-adsorption of MUA with shorter thiols yielded less crystalline-like thin films, but with more reactive carboxylic acid terminal...
Tipo: Text Palavras-chave: PM IRRAS; Antibody; Protein A; Gold; Self assembled monolayers.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2222.pdf
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Immunomodulatory properties of Alternanthera tenella Colla aqueous extracts in mice BJMBR
Guerra,R.N.M.; Pereira,H.-A.W.; Silveira,L.M.S.; Olea,R.S.G..
Plants from the genus Alternanthera are thought to possess antimicrobial and antiviral properties. In Brazilian folk medicine, the aqueous extract of A. tenella Colla is used for its anti-inflammatory activity. The present study investigated the immunomodulatory property of A. tenella extract by evaluating the antibody production in male albino Swiss mice weighing 20-25 g (10 per group). The animals received standard laboratory diet and water ad libitum. The effect of A. tenella extract (5 and 50 mg/kg, ip) was evaluated in mice immunized with sheep red blood cells (SRBC 10%, ip) as T-dependent antigen, or in mice stimulated with mitogens (10 µg, Escherichia coli lipopolysaccharide, LPS, ip). The same doses (5 and 50 mg/kg, ip) of A. tenella extract were...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Antitumor activity; Antibody; Immunomodulation; Ehrlich ascites; Alternanthera.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900011
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Impact of co-blocking the costimulatory signals on immune-related genes after high-risk rabbit corneal allograft using 2nd-generation DNA sequencing technology Genet. Mol. Biol.
Zhao,Hai-Xia; Li,Xin-Yu; Guan,Wen-Ying; Han,Xiao-Tong.
Abstract The aim of this study was to evaluate the impact and mechanism of co-blocking of costimulatory signals CD28-B7-CD40-CD40L during immune allograft rejection. Forty-eight recipient rabbits were prepared as a high-risk corneal allograft model. After surgery, the animals were randomly divided into: control group, MR1 group, anti-B7 group, and co-blocking group (n=12, each group). Subconjunctival injection was first performed on the allograft surgery day until post-surgery day five. Four weeks later, or when immune rejection occurred, the cornea was sampled to detect and analyze the gene spectrum. The survival time in the co-blocking group was significantly longer than that in the other three groups (p < 0.05). Gene expression analysis revealed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Costimulatory signal; Corneal allograft; Immune rejection; Second-generation sequencing technology; Antibody.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300472
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Inactivated Parapoxvirus ovis as inducer of immunity in silver catfish (Rhamdia quelen) Anais da ABC (AABC)
PAVAN,TATIANA R.; NIED,CRISTIAN O.; NORO,MIRELA; ANZILIERO,DENIZ; FRANDOLOSO,RAFAEL; KREUTZ,LUIZ CARLOS.
ABSTRACT Molecules with immune modulating activity are ubiquitously distributed in nature and their impact on aquaculture has been exploited in order to increase fish resistance to pathogens. Here, we investigated the effect of inactivated Parapoxvirus ovis (iPPVO) on blood cells and innate and acquired immune response of silver catfish (Rhamdia quelen). iPPVO inoculation had no effect on respiratory burst activity; however, following iPPVO inoculation, we observed a significant decrease on circulating monocytes concomitantly with an increased number of heterophilic granulocytes and thrombocytes, which are the main cells involved in innate immunity and provide connection with acquired immunity. Fish inoculated with a combination of bovine serum albumin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunostimulant; Adjuvant; Antibody; Fish.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401451
Registros recuperados: 31
Primeira ... 12 ... Última
 

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