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Registros recuperados: 21
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Activity of essential oils from spices against Staphylococcus spp. isolated from bovine mastitis Arq. Bras. Med. Vet. Zootec.
Dal Pozzo,M; Santurio,D.F; Rossatto,L; Vargas,A.C; Alves,S.H; Loreto,E.S; Viegas,J.
Avaliou-se a atividade antimicrobiana dos óleos essenciais (OE) de Origanum vulgare (orégano), Thymus vulgaris (tomilho), Lippia graveolens (lipia), Zingiber officinale (gengibre), Salvia officinalis (sálvia), Rosmarinus officinalis (alecrim) e Ocimum basilicum (manjericão), e de suas frações majoritárias, carvacrol e timol, frente a 32 isolados de Staphylococcus spp, oriundos de rebanhos leiteiros bovinos. A concentração inibitória mínima (CIM) e a concentração bactericida mínima foram determinadas por meio da técnica de microdiluição em caldo. Orégano, tomilho e lípia (Orégano Mexicano) apresentaram atividade antimicrobiana similar, médias geométrica de CIM de 1600µg mL-1; 1564µg mL-1; 1562µg mL-1, respectivamente, no entanto menos ativos que carvacrol,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plants; Phytotherapy; Antimicrobials; Plantas medicinais; Fitoterapia; Antimicrobianos.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000500026
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Antibacterial efficacy of nisin, bacteriophage P100 and sodium lactate against Listeria monocytogenes in ready-to-eat sliced pork ham BJM
Figueiredo,Ana Cláudia L.; Almeida,Rogeria C.C..
ABSTRACT The effectiveness of bacteriophage P100, nisin and sodium lactate, individually and in combination, in inhibiting Listeria monocytogenes in ready-to-eat pork ham slices was assessed. The antimicrobials were applied to the surfaces of ready-to-eat pork ham slices, which were inoculated with a mixture of L. monocytogenes. Among the individual antimicrobial treatments, bacteriophage P100 was the most effective, decreasing L. monocytogenes to undetectable levels at zero and 72 h post-infection. Sodium lactate was the least effective treatment. Treatment with nisin at zero h significantly reduced initial cell density (p < 0.05). However, this pattern was not observed at 72 h of storage. A significant difference (p < 0.05) existed between the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobials; Food safety; Meat products; Listeria monocytogenes.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400724
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Antimicrobial resistance, diarrheagenic and avian pathogenic virulence genes in Escherichia coli from poultry feed and the ingredients Arq. Bras. Med. Vet. Zootec.
Rossato,J.M.; Brito,B.G.; Kobayashi,R.K.T.; Koga,V.L.; Sarmiento,J.J.P.; Nakazato,G.; Lopes,L.F.D.; Balsan,L.A.G.; Grassotti,T.T.; Brito,K.C.T..
ABSTRACT Diarrheagenic (DEC) and avian pathogenic Escherichia coli (APEC) are associated with intestinal and extra-intestinal infections (ExPEC), respectively. We aimed to analyze the antimicrobial susceptibility, gene encoding virulence factors associated to DEC and APEC, and phylogenetic classification in E. coli isolated from 320 samples of feed and ingredients. Antimicrobial susceptibility was performed using the disk diffusion method and Multiple Antibiotic Resistance (MAR) Index and Multi-Drug Resistance (MDR) were calculated. Phylogenetic classification was performed on samples harboring DEC and/or APEC virulence-associated genes. A total of 110 E. coli strains were isolated in 15% (49/320) of the evaluated inputs (n=13 vegetable meal; n=33 animal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Poultry production; Animal feed; Antimicrobials; Virulence; Resistance.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601968
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Antimicrobial susceptibility and pathogenicity of Escherichia coli strains of environmental origin Ciência Rural
Carvalho,Daiane; Finkler,Fabrine; Grassotti,Tiela Trapp; Kunert Filho,Hiran Castagnino; Lima,Francisco Esmaile de Sales; Soares,Beatriz Dugaich; Rossato,Juliana Marzari; Cunha,Augusto César da; Brito,Kelly Cristina Tagliari de; Brito,Benito Guimarães de.
The study aimed to evaluate the antimicrobial susceptibility of 109 samples of Escherichia coli (E. coli) of environmental origin and to characterize these isolates according to the degree of pathogenicity in vivo, verifying a possible relationship between this variable and susceptibility to the active principles tested. The isolates were subjected to disc diffusion test to 14 antibiotics. From 16.5% to 90% of the samples were sensitive; 1 - 28.5% showed intermediate degree of susceptibility and between 9 to 78% of E. coli analyzed were resistant. The highest resistance percentages were seen in the class of quinolones and tetracyclines (&gt;75%), and for sensitivity in the class of amphenicols (68.8%). By inoculating 1- day - old chicks, the isolates...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; Resistance; Antimicrobials; Pathogenicity; Poultry..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701249
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Antimicrobiales para el control de hongos en una dieta artificial y su impacto en la supervivencia y reproducción de Spodoptera frugiperda (J. E. Smith). Colegio de Postgraduados
Zambrano Gutiérrez, Jorge.
Las dietas artificiales son importantes para establecer crías de insectos bajo condiciones controladas de laboratorio. Sin embargo, en estas condiciones también pueden la contaminación de las dietas con hongos. Para evitar la contaminación de las dietas de insectos se utilizan antimicrobiales que en algunas ocasiones ya están presentes en la dieta o bien se deben agregar a ésta. En el laboratorio de Toxicología de Insecticidas la dieta artificial BioServ Inc., Frenchtown, NJ. se contaminaba con hongos. Así mismo se observó que al controlar la contaminación, ésta se podría utilizar en la cría masiva de Spodoptera frugiperda. El objetivo general del presente trabajo fue estimar la supervivencia, reproducción, duración del ciclo de vida, peso de larvas de...
Palavras-chave: Contaminación; Dietas artificiales; Hongos; Cría masiva; Control; Antimicrobiales; Sobrevivencia; Reproducción; Contamination; Artificials diets; Fungi; Mass rearing; Control; Antimicrobials; Survival; Reproduction; Entomología y Acarología; Maestría.
Ano: 2013 URL: http://hdl.handle.net/10521/2034
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Bacteriocins: molecules of fundamental impact on the microbial ecology and potential food biopreservatives BABT
Souza,Evandro Leite de; Silva,Clemilson Antonio da; Sousa,Cristina Paiva de.
Bacteriocins are proteic molecules synthesized for various lineages of Gram-positive and Gram-negative bacteria when exposed to stressful conditions. Bacteriocins have been characterized as molecules of high antimicrobial property even at low concentrations, provoking the microbial survival inhibition by antibiosis. These substances have their synthesis mediated for genetic mechanisms and develop their lethal action on the microbial cell by multiples mechanisms that can act of isolated or concomitant way culminating with microbial cell killing. This molecules class presents characteristic of stability to heat, low pH, refrigeration and freezing, and resistance to weak organics solvents, salts and enzymes. On the other hand, they are very sensitive to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteriocins; Antimicrobials; Food Industry; Biopreservation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500008
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Composition and Bioactive Properties of Yerba Mate (llex paraguariensis A. St.-Hil.): A Review Chilean J. Agric. Res.
Burris,Kellie P; Harte,Federico M; Michael Davidson,P; Neal Stewart Jr,C; Zivanovic,Svetlana.
Yerba Mate is a popular tea beverage produced and consumed in the South American countries of Argentina, Brazil, Chile, Paraguay, and Uruguay, and is processed from the leaves and stems of Ilex paraguariensis A. St.-Hil., a perennial shrub from the Aquifoliaceae family. Production occurs in six stages: harvesting older leaves and small stems, roasting by direct fire, drying under hot air, milling to specified size, aging to acquire optimal sensory attributes, and final packaging. While grown and consumed for centuries in South America, its popularity is increasing in the United States because of demand by consumers for healthier, more natural foods, its filling a niche for a different type of tea beverage, and for Yerba Mate's potential health benefits -...
Tipo: Journal article Palavras-chave: Antioxidant; Antimicrobials; Natural products; Yerba mate; Tea.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000200016
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Effect of Antimicrobials on Salmonella Spp. Strains Isolated from Poultry Processing Plants Rev. Bras. Ciênc. Avic.
Mion,L; Parizotto,L; Calasans,M; Dickel,EL; Pilotto,F; Rodrigues,LB; Nascimento,VP; Santos,LR.
ABSTRACT The routine use of antimicrobials in animal production for the treatment of infections, disease prevention, or as growth promoters is a predisposing factor for the development and dissemination of antimicrobial resistance. In food industries, sanitizers are used for the control of microbial colonization, and their efficacy depends on contact time and on the dilution of the products used. The present study assessed the effect of 12 antimicrobials and four commercial sanitizers on 18 Salmonella spp. strains isolated from poultry processing plants. None of the evaluated antimicrobials was 100% effective against the tested Salmonella spp. strains; however, 94% of the isolates were susceptible to ciprofloxacin, 77% to amoxicillin + clavulanic acid and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobials; Poultry; Sanitizers; Salmonella spp.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200337
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Especies novedosas de champiñones (Agaricus) con propiedades funcionales antioxidantes y antimicrobianas, aisladas de zonas rurales de México. Colegio de Postgraduados
Cruz Romero, Elizabeth.
México cuenta con una gran diversidad biológica, sin embargo, poco se han aprovechado los recursos genéticos nativos para la producción comercial de hongos comestibles. Los champiñones, son los hongos comestibles más comercializados en el mercado mexicano para lo cual se usan cepas extranjeras. La presente investigación se inició con el estudio preliminar de 15 cepas nativas de México de Agaricus spp. Dado que la investigación requería de biomasa micelial, al final solo se trabajó con dos cepas de Agaricus spp. (CP-83 y CP-89). Al micelio de estas cepas se les determinaron antioxidantes y pruebas antimicrobianas. Estas pruebas se realizaron de macerados y extractos acuosos y etanólico. La prueba antimicrobiana se realizo con una cepa de E. coli (CPB-8). En...
Palavras-chave: Agaricus; Micelio; Cultivo de hongos comestibles; Antioxidantes; Antimicrobianos; E. coli; Agaricus mycelia; Cultivation of edible mushrooms; Antimicrobials; EDAR; Estrategias para el Desarrollo Agrícola Regional; Maestría; Antioxidants.
Ano: 2012 URL: http://hdl.handle.net/10521/675
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Etiology of canine otitis media and antimicrobial susceptibility of coagulase-positive Staphylococci in Fortaleza city, Brazil BJM
Oliveira,Lis Christina de; Leite,Carlos Artur Lopes; Brilhante,Raimunda Sâmia N.; Carvalho,Cibele Barreto M..
This study evaluated the middle ear microbiota and antimicrobial susceptibility patterns from strains isolated from dogs with otitis media. A total of 62 dogs obtained from Zoonoses Control Center of Fortaleza City Ceará State / Brazil were studied over a 10-month period (August/2003 to June/2004). Of the total, 46.8% (n=30) of the animals were positive for otitis media and the infection was monomicrobial in 76.6% of them. The most frequent isolated agents were coagulase-positive Staphylococci (CPS-55%) and Pseudomonas sp (10%). For S. intermedius (n=13) and S. aureus subsp aureus (n=9), respectively, the greater resistance rates were observed using penicillin G (30.76% and 44.44%), ampicillin (7.69% and 44.44%), erythromycin (23.07% and 44.44%),...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Otitis media; Dogs; Staphylococci; Antimicrobials.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200009
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Evaluation of antibiotics as a methodological procedure to inhibit free-living and biofilm bacteria in marine zooplankton culture Anais da ABC (AABC)
Agostini,Vanessa O.; Macedo,Alexandre J.; Muxagata,Erik.
There is a problem with keeping culture medium completely or partially free from bacteria. The use of prokaryotic metabolic inhibitors, such as antibiotics, is suggested as an alternative solution, although such substances should not harm non-target organisms. Thus, the aim of this study was to assess the effectiveness of antibiotic treatments in inhibiting free-living and biofilm bacteria and their half-life in artificial marine environment using the copepod Acartia tonsa as bioindicador of non-harmful antibiotic combinations. Regarding to results, the application of 0.025 g L-1 penicillin G potassium + 0.08 g L-1 streptomycin sulphate + 0.04 g L-1 neomycin sulphate showed great potential for use in marine cultures and scientific experiments without...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acartia tonsa; Antimicrobials; Experiment; Marine culture; Toxicity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200733
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Garlic essential oil increases rates of eggs fertilization and hatching of Rhamdia quelen larvae in an artificial incubation system Ciência Rural
Marengoni,Nilton Garcia; Pereira,Letícia Angélica; Weiss,Luciane Aline; Besen,Mateus Antonio; Bombardelli,Robie Allan.
ABSTRACT: The objective of this study was to evaluate the effects of garlic (Allium sativum) essential oil on the rates of eggs fertilization and hatching and on the normal development of larvae of artificially incubated silver catfish (Rhamdia quelen). The experiment was carried out using a completely randomized experimental design with four treatments and five replicates. The treatments consisted of introducing garlic essential oil into the incubators, which used a closed system of water recirculation, at concentrations of 0, 1, 3, and 5mg L-1. Rates of eggs fertilization and larval hatching were determined at 10 and 24h after the beginning of eggs hydration. Morphology of larvae was assessed with the aid of a stereo microscope to determine the rate of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Silver catfish; Antimicrobials; Hatching rate; Larvae; Allium sativum.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600652
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Green and brown propolis: efficient natural biocides for the control of bacterial contamination of alcoholic fermentation of distilled beverage Ciênc. Tecnol. Aliment.
Mutton,Márcia Justino Rossini; Oliveira Filho,José Humberto de; Costa,Gustavo Henrique Gravatim; Roviero,Juliana Pelegrini; Freita,Lidyane Aline de.
This study aimed to evaluate the efficiency of natural biocides, brown and green propolis, for the control of bacterial contamination in the production of sugarcane spirit. The treatments consisted of brown and green propolis extracts, ampicillin, and a control and were assessed at the beginning and end of harvest season in ten fermentation cycles. In the microbiological analyses, the lactic acid bacteria were quantified in the inoculum before and after the treatment with biocides, and the viability of yeast cells during fermentation was evaluated. The levels of acids, glycerol, total residual reducing sugars, and ethanol were analyzed for the wine resulting from each fermentation cycle. A reduction in the number of bacterial contaminants in the inoculum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sugarcane spirit; Antimicrobials; Natural products; Lactic acid bacteria; Ethanol.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000400018
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Hazards in non-pasteurized milk on retail sale in Brazil: prevalence of Salmonella spp, Listeria monocytogenes and chemical residues BJM
Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Beloti,Vanerli; Barros,Marcia A.F.; Netto,Daisy Pontes; Pinto,José Paes A.N.; Andrade,Nélio José de; Silva,Wladimir P.; Franco,Bernadette D.G.M..
Fluctuations in the Brazilian milk market force small milk producers to find temporary trade alternatives, which include selling raw milk to people who prefer this type of milk rather than heat-processed milk. Considering the importance of these small milk producers to the market and the well-known health risks associated to consumption of raw milk, this study evaluated the microbiological quality and the presence of Listeria monocytogenes, Salmonella spp., chlorine, antimicrobials and insecticides (organophosphates and carbamates) in raw milk produced in 210 small and medium farms located in four important milk-producing Brazilian states (Paraná, São Paulo, Minas Gerais and Rio Grande do Sul). In 66% of the selected farms the milking was manual. In 33% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hazards; Raw milk; Salmonella; Listeria monocytogenes; Hygiene indicators; Insecticides; Antimicrobials.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200007
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Isolation of Staphylococcus epidermidis from inflamed upper respiratory tract of an orange-spined hairy dwarf porcupine (Sphiggurus villosus) J. Venom. Anim. Toxins incl. Trop. Dis.
Fornazari,F; Guimarães,FF; Teixeira,CR; Langoni,H.
The orange-spined hairy dwarf porcupine (Sphiggurus villosus) is a rodent species common in most parts of South America, and little is known about the pathologies that can afflict it. A specimen was delivered at the Wildlife Research and Medical Center (CEMPAS), School of Veterinary Medicine and Animal Husbandry, UNESP, Botucatu, SP, Brazil. The animal showed intense apathy, with purulent secretion in the nasal cavity and fracture of the lumbar spine. Due to the unfavorable prognosis, the porcupine was euthanized and microbiological culture of nasal discharge showed Staphylococcus epidermidis. The antimicrobial resistance test revealed sensitivity to all tested antimicrobials (ampicillin, oxacillin, tetracycline, penicillin G, neomycin, cephalexin,...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Staphylococcus epidermidis; Orange-spined hairy dwarf porcupine; Sphiggurus villosus; Antimicrobials; Susceptibility.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400016
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Membrane damage of bacteria by silanols treatment Electron. J. Biotechnol.
Kim,Yun-mi; Farrah,Samuel; Baney,Ronald H.
Antimicrobial action of silanols, a new class of antimicrobials, was investigated by transmission electron microscopy and fluorescent dye studies. Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa and Gram-positive bacteria, Staphylococcus aureus and Enterococcus faecalis were treated by silanols at concentration of less than 0.2 wt% for one hour. Membrane damage of the bacteria by the silanol treatment was clearly observed by transmission electron microscopy. Separation of the cytoplasmic membrane from the outer membrane for E. coli and disorganized cytoplasmic membrane of the Gram-positive bacteria were observed when compared to the control. Fluorescent dyes, green-fluorescent nucleic acid stain (Syto 9) and the red-fluorescent nucleic...
Tipo: Journal article Palavras-chave: Antimicrobials; Hydrophobicity; Organosilicon compounds.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200009
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Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome broiler carcasses BJM
Moreira,Maria Aparecida S.; Rodrigues,Patrícia P.C.F.; Tomaz,Rafael S.; Moraes,Célia A. de.
Members of the Enterobacteriaceae family are present in the intestines of man and animals as commensals or are important disease causing agents. Bacteria bearing multidrug efflux systems (MDR) are able to survive adverse ecological niches. Multiresistant Escherichia coli and Enterobacter cloacae isolates from wholesome broiler carcasses were investigated for the presence of MDR. Lowering of Minimal Inhibitory Concentration for antimicrobials in the presence of a proton-motive force (PMF) uncoupler was tested as a potential display of the MDR phenotype. PCR amplification of the genes encoding AcrA and AcrB, components of a MDR system was performed. Diversity of each species was ascertained by Pulsed-Field Gel Electrophoresis (PFGE) of DNA digested with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multiresistance; Antimicrobials; Enterobacteriaceae; Proton-motive force; Diversity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200007
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Multidrug efflux systems in Gram-negative bacteria BJM
Moreira,Maria Aparecida Scatamburlo; Souza,Edmar Chartone de; Moraes,Célia Alencar de.
Multidrug efflux mechanisms in bacteria contribute significantly to intrinsic and acquired resistance to antimicrobial agents. Genome analysis have confirmed the broad distribution of these systems in Gram-negative as well as in Gram-positive bacteria. Among resistance mechanisms, the multidrug efflux system or pump deserves special attention, since a cell that has acquired it can simultaneously diminish or even suppress the susceptibility to a wide range of antimicrobials. The efflux system is mediated by transport proteins which confer resistance to toxic compounds. In Gram-negative bacteria, a tripartite efflux system is necessary to expel the drug to the outer medium: a protein localized in the cytoplasmic membrane; another in the periplasmatic space...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gram-negative bacteria; Multidrug efflux systems; Antimicrobials; Transport proteins; Multiresistance.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100003
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Propolis extract in tshe diet of weaned piglets Ciência Rural
Gonçalves,Liliane Maria Piano; Kiefer,Charles; Silva,Camilla Mendonça; Leal,Cássia Rejane Brito; Alencar,Stephan Alexander da Silva; Carvalho,Kelly Cristina Nunes; Rufino,Luciana Moura.
ABSTRACT: This study was conducted to assess the use of propolis ethanolic extract as an alternative to performance-enhancing antibiotics for piglets. Seventy piglets weaned at 21 days of age, with initial weights of 7.3±0.4kg and final weights of 24.9±1.2kg, were randomly assigned to five diets: positive control (diet with 200ppm antibiotic); negative control (diet without growth promoter); and diets with 100, 500, and 1,000ppm of brown propolis ethanolic extract, with seven replicates of two piglets each. The duration of the experimental period was 35 days. The diets did not influence (P>0.05) daily feed intake, daily weight gain, final weight, and feed conversion of animals. The bacteriological profile of the nasal swab presented a predominance of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobials; Diarrhea; Ethanolic extract; Vegetable extract.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000100702
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Resistance profile to antimicrobials of Salmonella spp. isolated from human infections BABT
Delicato,Elaine Regina; Mikcha,Jane Martha Graton; Fernandes,Sueli Aparecida; Pelayo,Jacinta Sanchez.
The purpose of this study was to analyse the profile of antimicrobial resistance among 21 strains of Salmonella isolated from patients with gastroenteritis symptom. It was observed that S. enteritidis was the serotype prevalent. These strains were sensitive to the majority of the antimicrobials tested, however, high resistance was observed in S. typhimurium and S. enterica subsp. enterica serotype 4,5,12:i:-. Surveillance and an efficient monitoring should be priority for the public health for the containment of antimicrobial resistance in foodborne infections.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Humans; Serotypes; Resistance; Antimicrobials.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200005
Registros recuperados: 21
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