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Acumulación de ácido araquidónico en fosfolípidos y triglicéridos y producción de prostaglandinas con relación a estrés térmico en semilla de ostión Crassostrea gigas ArchiMer
Duran Encinas, Yazmin.
Crassostrea gigas is a marine invertebrate that inhabits the intertidal zone and therefore, is commonly exposed to multiple environmental stressors, including temperature. In recent years mass mortality events have been reported in Mexico in C. gigas associated with the increase in temperature during the summer. Organisms under thermal stress present an increase in prostaglandins (PG) production by cyclooxygenases (COX), an enzymatic route using arachidonic acid (ARA) present in the phospholipids (FL) of the cell membrane as substrate, but PG can also increase non-enzymatically by reactive oxygen species (ROS) that are produced during increased metabolism. It has been reported that some organisms exposed to stress accumulate a higher concentration of ARA...
Tipo: Text Palavras-chave: Arachidonic acid; Stress; Phospholipid; Isotope; Mollusk; Temperature; Triglyceride; Prostaglandin.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00620/73193/72387.pdf
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Anti-inflammatory and antispasmodic activity of Ipomoea imperati (Vahl) Griseb (Convolvulaceae) BJMBR
Paula,A.C.B.; Hayashi,L.S.S.; Freitas,J.C..
Ipomoea imperati (Convolvulaceae) lives on the sandy shores of the Brazilian coast and in other areas of the world. The anti-inflammatory activity of a methanol-water extract of the leaves of I. imperati was investigated in experimental models of acute and subchronic inflammation. Topical application of the extract (10 mg/ear) inhibited mouse ear edema induced by croton oil (89.0 ± 1.3% by the lipid fraction with an IC50 of 3.97 mg/ear and 57.0 ± 1.3% by the aqueous fraction with an IC50 of 3.5 mg/ear) and arachidonic acid (42.0 ± 2.0% with an IC50 of 4.98 mg/ear and 31.0 ± 2.0% with an IC50 of 4.72 mg/ear). Phospholipase A2, purified from Apis mellifera bee venom, was also inhibited by the extract (5.0 mg/ml lipid and aqueous fraction) in vitro in a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anti-inflammatory activity; Antispasmodic activity; Arachidonic acid; Croton oil; Medicinal plants; Phospholipase A2.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100014
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Arachidonic acid affects biofilm formation and PGE2 level in Candida albicans and non-albicans species in presence of subinhibitory concentration of fluconazole and terbinafine BJID
Mishra,Nripendra Nath; Ali,Shakir; Shukla,Praveen K..
Candida albicans utilizes arachidonic acid (AA) released during the course of infection (Candidiasis) from phospholipids of infected host cell membranes and synthesizes extracellular prostaglandin(s) which play an important role in hyphae formation and host cell damage. C. albicans biofilms secrete significantly more prostaglandin(s) and evidence suggests that Candida biofilms have dramatically reduced susceptibility to majority of antifungal drugs. AA influences the saturation level of lipids and fluidity of yeast cell membranes. Therefore the aim of this study was to evaluate the effect of AA alone or in combination with antifungal agents on biofilm formation and production of prostaglandin (PGE2) in C. albicans, C. parapsilosis, C. glabrata, C....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachidonic acid; Candida albicans; AmBR; Candida tropicalis; Prostaglandin E2; FDA.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300287
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Arachidonic acid triggers an oxidative burst in leukocytes BJMBR
Pompeia,C.; Cury-Boaventura,M.F.; Curi,R..
The change in cellular reducing potential, most likely reflecting an oxidative burst, was investigated in arachidonic acid- (AA) stimulated leukocytes. The cells studied included the human leukemia cell lines HL-60 (undifferentiated and differentiated into macrophage-like and polymorphonuclear-like cells), Jurkat and Raji, and thymocytes and macrophages from rat primary cultures. The oxidative burst was assessed by nitroblue tetrazolium reduction. AA increased the oxidative burst until an optimum AA concentration was reached and the burst decreased thereafter. In the leukemia cell lines, optimum concentration ranged from 200 to 400 µM (up to 16-fold), whereas in rat cells it varied from 10 to 20 µM. Initial rates of superoxide generation were high,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachidonic acid; NADPH oxidase; Nitroblue tetrazolium; Leukocytes; Reactive oxygen species.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100013
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Characterization of the ovary fatty acids composition of Rhamdia quelen (Quoy & Gaimard) (Teleostei: Siluriformes), throughout their reproductive cycle Neotropical Ichthyology
Anido,Rodrigo Vargas; Zaniboni-Filho,Evoy; Garcia,Alexandre Sachsida; Baggio,Sueli Regina; Fracalossi,Débora Machado.
Knowledge about gonad fatty acid composition is important for broodstock diet formulation. This study characterized ovary fatty acid composition of wild female jundiá catfish (Rhamdia quelen) in their different gonadal maturation stages. Female jundiá (n = 36, average weight= 383.8 + 208.8 g) were captured in the rio Uruguay, comprising all seasons. Ovaries were extracted and classified according to their gonadal maturation stage. Gonad-somatic ratio varied significantly among seasons, being higher in spring (3.7), followed by summer (2.2), winter (0.9) and autumn (0.6). Main fatty acids groups detected were: saturated (SFA= 35.5%), monounsaturated (MUFA= 28.1%) and polyunsaturated fatty acids (PUFA= 33.5%). Over the four seasons, palmitic acid was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachidonic acid; Broodstock diet; Docosahexaenoic acid; Eicosapentaenoic acid; Gonadal maturation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000200453
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Effect of arachidonic acid supplementation and cyclooxygenase/lipoxygenase inhibition on the development of early bovine embryos R. Bras. Zootec.
Pereira,Rosa Maria; Marques,Carla Cruz; Baptista,Maria da Conceição; Vasques,Maria Irene; Horta,António Eduardo.
The effect of arachidonic acid (AA) cascade on bovine embryo development in a granulosa cell co-culture system was studied. Arachidonic acid (100 µM) was supplemented from 1-cell to 8-16 cell block stage (first three days of co-culture) and from 1-cell to hatching. Specific cyclooxygenase (indomethacin, 28 µM) and lipoxygenase (nordihydroguaiaretic acid - NDGA, 28 µM) inhibitors were used from 1-cell to 8-16 cell block stage with AA. Embryo development was assessed by cleavage, day 7-day 8 and hatched embryo rates and by measuring growth rates through development stages found in days 7-10 of culture (day 0 = insemination day). Embryo quality was scored at day 8. A 6.5-10.4% increase on cleavage rate after AA supplementation was found. This AA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachidonic acid; Bovine embryos; Granulosa; In vitro.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982006000200012
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Effect of dietary fat saturation on lipid metabolism, arachidonic acid turnover and peritoneal macrophage oxidative stress in mice BJMBR
Oliveros,L.B.; Videla,A.M.; Giménez,M.S..
We investigated the effects of a saturated fat diet on lipid metabolism and arachidonic acid (AA) turnover in mouse resident peritoneal macrophages. The pro-oxidative effect of this diet was also studied. Female C57BL/6 mice were weaned at 21 days of age and assigned to either the experimental diet containing coconut oil (COCO diet), or the control diet containing soybean oil as fat source (10 mice per group). The fat content of each diet was 15% (w/w). Mice were fed for 6 weeks and then sacrificed. The concentration of total lipids, triglycerides, (LDL + VLDL)-cholesterol, thiobarbituric acid-reactive substances (TBARS) and reduced glutathione were increased in the plasma of mice fed the COCO diet, without changes in phospholipid or total cholesterol...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peritoneal macrophage; Coconut oil; Soybean oil; Lipids; Oxidative stress; Arachidonic acid.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300004
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Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68 BJM
Samadlouie,Hamid-Reza; Hamidi-Esfahani,Zohreh; Alavi,Seyed-Mehdi; Varastegani,Boshra.
The time courses for production of fungal biomass, lipid, phenolic and arachidonic acid (ARA) as well as expression of the genes involved in biosynthesis of ARA and lipid were examined in Mortierella alpina CBS 754.68. A significant increase in the arachidonic acid content in lipids that coincided with reduced levels of lipid was obtained. Reduced gene expression occurred presumably due to the steady reduction of carbon and nitrogen resources. However, these energy resources were inefficiently compensated by the breakdown of the accumulated lipids that in turn, induced up-regulated expression of the candidate genes. The results further indicated that the expression of the GLELO encoding gene is a rate-limiting step in the biosynthesis of ARA in the early...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mortierella alpina; Arachidonic acid; Lipid; Gene expression; Real time PCR.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200010
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Fatty acid composition of the catfish -1: Heterobranchus longifillis (Pisces: clariidae and Chyrsichthys nigrodigitatus (Pisces: Bagridae) reared in tropical fresh waters OceanDocs
Omuaru, V.; Idoniboye-Obu, T.I.E..
The relationship of the fatty acid composition of the three developmental stages (fingerling, juvenile and adult) of Heterobranchus longifilis (Pisces: Clariidae) and Chrysichthys nigrodigitatus (Pisces: Bagridae) have been evaluated by gas liquid chromatography (Glc) on polyethylene glycol-2-nitroterephthalic ester (PEGNTE). Results indicated that adults of these fishes, accumulated linolenic acid (C18:3w3) above dietary levels atthe fingerling stage which was remarkably depleted in the adult fishes. Its essentiality in their nutrition and metabolism is thus suggested. In the metabolic tumover,.C18:3w3, is utilized to build up the store of eicosapentaenoic acid (C20:5w3), inHeterobranchus longifillis while the store of eicosapentaenoic acis (C20:5w3),...
Tipo: Journal Contribution Palavras-chave: Palmitic acid; Docosatetraenoic acid; Gas liquid chromatography; Eicosapentaenoic acid; Oleic acid; Polyethylene glycol-2-nitroterephthalic ester; Linolenic acid; Arachidonic acid; Fish species.
Ano: 1998 URL: http://hdl.handle.net/1834/646
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Lipid composition of the giant lion's-paw scallop (Nodipecten subnodosus) in relation to gametogenesis - I. Fatty acids ArchiMer
Palacios, Elena; Racotta, I; Kraffe, E; Marty, Y; Moal, Jeanne; Samain, Jean-francois.
Seasonal variations in fatty acid composition in several tissues of a 1-year-old population of giant lion's-paw scallop Nodipecten subnodosus were analyzed during gonad development. Samples were taken bimonthly from December 1999 to June 2000, a period in which gonad development was occurring. Fatty acid accumulation in neutral and polar lipids of the female gonad was concomitant to the gonad development and presented a maximum in June. Among essential fatty acids, a specific accumulation was observed only for 20:5n - 3 in the female gonad, as suggested by an increase in its proportion in the neutral and the polar fractions. However, no specific accumulation was observed for 22:6n - 3, as its proportion remained relatively constant in both fractions. We...
Tipo: Text Palavras-chave: Reproduction; Pectinidae; HUFA; EPA; DHA; Arachidonic acid.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-767.pdf
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Lipid oxidation and fatty acid profile related to broiler breast meat color abnormalities BABT
Soares,Adriana Lourenço; Marchi,Denis Fabrício; Matsushita,Makoto; Guarnieri,Paulo Donizeti; Droval,Adriana Aparecida; Ida,Elza Iouko; Shimokomaki,Massami.
The aim of this work was to study the influence of lipid oxidation on broiler breast meat (Pectoralis major m) color abnormalities. There were 27.0 % more lipid oxidation in PSE in relation to normal meat and 41.0 % more in relation to DFD-like meat (p<0.05). The fatty acid profile was also significantly different since the arachidonic acid (AA) fraction increased 38.6 and 70.5 % in PSE meat comparing to normal and DFD-like meats, respectively. The ratio PUFA/SFA changed in these three types of meat: 0.736, 0.713 and 0.694 for PSE, normal and DFD-like meat respectively, reflecting the highest production of polyunsaturated fatty acids in the PSE meat samples. Results indicated that phospholipase A2 enzyme activities played a relevant role towards the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PSE meat; DFD meat; Arachidonic acid.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600023
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Omega 6 and 3 polyunsaturated fatty acids dog and cat nutrition MV&Z
Carciofi, Aulus Cavalieri; Bazolli, Rodrigo Sousa; Prada, Flávio.
between pro-inflammatory PUFA cis-linoleic (w6) and acachidonic (w6), versus the less inflammatory PUFA alpha-linolenic (w3), gamma-linolenic (w6), eicosapentaenoic (w3) and docosaeicosahenoic(w3) determines the fami1y of leukotrienes, prostaglandins and thromboxanes produced in the tissues, resulting in more or less inflammation (vasodilatation, aggregation, painful, etc.). Clinical studies have indicated positive effect of dietary relationship ofw6:w3 varying from 10:1to 2: I in inflammatory and coetaneous allergic diseases, arthritis, some tumors, kidney and cardiac disease, indicating a promising way to be explored.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polyunsaturated fatty acids; Arachidonic acid; Linolenic acid; Dog; Cat; Inflammation ácido graso poliinsaturado; Acido aracdónico; Ácido linolénico; Perro; Gato; Inflamación ácido graxo poliinsaturado; Ácido aracdônico; Ácido linolênico; Cão; Gato; Inflamação.
Ano: 2002 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3293
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Techniques for delivery of arachidonic acid to Pacific oyster, Crassostrea gigas, spat ArchiMer
Seguineau, Catherine; Soudant, P; Moal, Jeanne; Delaporte, Maryse; Miner, Philippe; Quere, Claudie; Samain, Jean-francois.
The present study tested two techniques for dietary supplementation of Crassostrea gigas spat with PUFA, such as arachidonic acid (AA). The first technique consisted of a preliminary enrichment and growth of an algal concentrate (T-ISO, Isochrysis sp.) with AA dissolved in an ethanol solution, the whole culture then being fed to the spat. This enrichment increased the AA weight percentage in T-ISO neutral and polar lipids from 0.6 to 22.4% and from 0.4 to 6.8%, respectively. The second delivery technique was direct addition separately of free AA dissolved in ethanol solution and algal concentrate (T-ISO + AA) to the spat-rearing tank. To test the efficiency of these delivery techniques, oyster spat were supplemented with AA-enriched T-ISO, T-ISO + AA, and...
Tipo: Text Palavras-chave: Food supply; Arachidonic acid; Spat; Crassostrea gigas; Dietary.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1094.pdf
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