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Registros recuperados: 19
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A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry BJMBR
Alves,L.P.S.; Almeida,A.T.; Cruz,L.M.; Pedrosa,F.O.; de Souza,E.M.; Chubatsu,L.S.; Müller-Santos,M.; Valdameri,G..
The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) production by the diazotrophic and plant-associated bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. The method consists of three steps: i) cell permeabilization, ii) Nile red staining, and iii) analysis by flow cytometry. The method was optimized step-by-step and can be carried out in less than 5 min. The final results indicated a high correlation coefficient (R2=0.99) compared to a standard method based on methanolysis and GC. This method...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flow cytometry; Nile red; Poly-3-hydroxybutyrate; Herbaspirillum seropedicae; Azospirillum brasilense.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100606
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Características agronômicas do trigo em função de Azospirillum brasilense, ácidos húmicos e nitrogênio em casa de vegetação AGRIAMBI
Rodrigues,Luan F. O. S.; Guimarães,Vandeir F.; Silva,Mônica B. da; Pinto Junior,Artur S.; Klein,Jeferson; Costa,Andreia C. P. R. da.
Objetivou-se, neste estudo, verificar o efeito do uso combinado de Azospirillum brasilense, ácidos húmicos e diferentes doses de nitrogênio nas características agronômicas do trigo. Os experimentos foram instalados no dia 21 de maio de 2011 e a colheita foi procedida no dia 13 de setembro de 2011. O delineamento experimental adotado foi o de blocos casualizados em esquema fatorial 4 x 4, com quatro repetições. O primeiro fator se refere à combinação da estirpe Ab-V5 de A. brasilense com os ácidos húmicos, sendo utilizados os seguintes tratamentos: controle; Ab-V5; ácido húmico e Ab-V5+ácido húmico e o segundo fator se refere às doses de nitrogênio (0, 30, 60 e 90 kg ha-1 N). A maioria das variáveis em análise apresentou efeito linear positivo dos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum L.; Azospirillum brasilense; Substâncias húmicas.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662014000100005
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Chemical characterization of wheat kernels naturally contaminated by deoxynivalenol-DON when cultivated under nitrogen management strategies Rev. Ciênc. Agron.
Souza,Thiago Montagner; Prando,André Mateus; Takabayashi-Yamashita,Cassia Reika; Zucareli,Claudemir; Hirooka,Elisa Yoko.
ABSTRACT Chemical composition and contamination of wheat kernels (Triticum aestivum L.) directly affect the quality of the flour obtained from them, determining its acceptability and use by industry. Field trials were conducted to evaluate the effect of agricultural practices on wheat kernel quality (chemical composition and contamination). Cultivation was carried out following maize or soybean (crop succession), with application of nitrogen doses in topdressing (0, 30, 60, 90, and 120 kg ha-1), using seeds inoculated or not inoculated with Azospirillum brasilense. Data were subjected to analysis of variance (ANOVA), comparison of means by Tukey's test (<0.05), and regression to nitrogen doses. Crop succession resulted in changes in kernel chemical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum (L.); Crop succession; Nitrogen doses; Azospirillum brasilense; Kernel composition.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000400650
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Comparación entre coinoculación con Bradyrhizobium japonicum y Azospirillum brasilense e inoculación simple con Bradyrhizobium japonicum en la nodulación, crecimiento y acumulación de N en el cultivo de soja Agriscientia (Córdoba)
Benintende,S.; Uhrich,W.; Herrera,M.; Gangge,F.; Sterren,M.; Benintende,M..
El objetivo fue comparar la coinoculación con Bradyrhizobium japonicum y Azospirillum brasilense con relación a la inoculación simple con Bradyrhizobium japonicum en la nodulación, crecimiento y acumulación de N en el cultivo de soja y su rendimiento. Se realizaron dos experimentos en lotes en los que nunca se cultivó soja en años con diferentes condiciones hídricas. Los tratamientos fueron: testigo (sin inoculación), coinoculación con Bradyrhizobium japonicum y Azospirillum brasilense e inoculación simple con Bradyrhizobium japonicum. La nodulación en ambos experimentos fue superior en la coinoculación, aunque hubo diferencias significativas en el experimento con déficit hídrico. Los rendimientos, en ambos experimentos, presentaron valores medios...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coinoculación; Soja; Bradyrhizobium japonicum; Azospirillum brasilense.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2010000200002
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Compatibility of Azospirillum brasilense and Pseudomonas fluorescens in growth promotion of groundnut ( Arachis hypogea L.) Anais da ABC (AABC)
PRASAD,ANDHARE A.; BABU,SUBRAMANIAN.
ABSTRACT We attempted to study the compatibility among plant beneficial bacteria in the culture level by growing them near in the nutrient agar plates. Among all the bacteria tested, Rhizobium was found to inhibit the growth of other bacteria. From the compatible group of PGPR, we have selected one biofertilizer (Azospirillum brasilense strain TNAU) and one biocontrol agent (Pseudomonas fluorescens strain PF1) for further studies in the pot culture. We have also developed a bioformulation which is talc powder based, for individual bacteria and mixed culture. This formulation was used as seed treatment, soil application, seedling root dip and foliar spray in groundnut crop in vitro germination conditions. A. brasilense was found to enhance the tap root...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum brasilense; Groundnut; PGPR; Pseudomonas fluorescens.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301027
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Different responses of the GlnB and GlnZ proteins upon in vitro uridylylation by the Azospirillum brasilense GlnD protein BJMBR
Araújo,L.M.; Huergo,L.F.; Invitti,A.L.; Gimenes,C.I.; Bonatto,A.C.; Monteiro,R.A.; Souza,E.M.; Pedrosa,F.O.; Chubatsu,L.S..
Azospirillum brasilense is a diazotroph found in association with important agricultural crops. In this organism, the regulation of nitrogen fixation by ammonium ions involves several proteins including the uridylyltransferase/uridylyl-removing enzyme, GlnD, which reversibly uridylylates the two PII proteins, GlnB and GlnZ, in response to the concentration of ammonium ions. In the present study, the uridylylation/deuridylylation cycle of A. brasilense GlnB and GlnZ proteins by GlnD was reconstituted in vitro using the purified proteins. The uridylylation assay was analyzed using non-denaturing polyacrylamide gel electrophoresis and fluorescent protein detection. Our results show that the purified A. brasilense GlnB and GlnZ proteins were uridylylated by...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Azospirillum brasilense; Nitrogen fixation; PII-like protein; GlnD; GlnB; GlnZ.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400006
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Effect of ATP and 2-oxoglutarate on the in vitro interaction between the NifA GAF domain and the GlnB protein of Azospirillum brasilense BJMBR
Sotomaior,P.; Araújo,L.M.; Nishikawa,C.Y.; Huergo,L.F.; Monteiro,R.A.; Pedrosa,F.O.; Chubatsu,L.S.; Souza,E.M..
Azospirillum brasilense is a diazotroph that associates with important agricultural crops and thus has potential to be a nitrogen biofertilizer. The A. brasilense transcription regulator NifA, which seems to be constitutively expressed, activates the transcription of nitrogen fixation genes. It has been suggested that the nitrogen status-signaling protein GlnB regulates NifA activity by direct interaction with the NifA N-terminal GAF domain, preventing the inhibitory effect of this domain under conditions of nitrogen fixation. In the present study, we show that an N-terminal truncated form of NifA no longer required GlnB for activity and lost regulation by ammonium. On the other hand, in trans co-expression of the N-terminal GAF domain inhibited the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum brasilense; NifA protein; GlnB protein; GAF domain; Nitrogen fixation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200005
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Effect of Inoculation with Rhizobacteria and Arbuscular Mycorrhizal Fungi on Growth and Yield of Capsicum chinense Jacquin JARTS
Constantino, M.; Gómez-Álvarez, R.; Álvarez-Solís, J. D.; Geissen, V.; Huerta, E.; Barba, E..
We evaluated the effect of two rhizobacteria (Azotobacter chroococcum and Azospirillum brasilense) and a commercial product containing multiple strains of arbuscular mycorrhizal fungi (AMF) and an NPK fertiliser on the growth and yield of habanero chilli (Capsicum chinense Jacquin). All treatments were applied as single or combined inoculants, under nursery and field conditions, in a completely randomised design. The biofertilisers were applied to the roots by coating or dipping, with the inoculants in a solid or liquid support, respectively. At 30 days after inoculation, populations of 2.5×106 to 1.3×106 cfu g soil−1 of A. brasilense and 10.3×105 to 2.6×105 of A. chroococcum were detected in the rhizosphere of the crop. The prevalence of colonisation of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agronomy and Crop Science Biofertiliser; Azospirillum brasilense; Azotobacter chroococcum; PGPR; Root coating.
Ano: 2008 URL: http://www.jarts.info/index.php/jarts/article/view/67
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Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense BJMBR
Nishikawa,C.Y.; Araújo,L.M.; Kadowaki,M.A.S.; Monteiro,R.A.; Steffens,M.B.R.; Pedrosa,F.O.; Souza,E.M.; Chubatsu,L.S..
Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize. The expression of genes responsible for nitrogen fixation (nif genes) in this bacterium is dependent on the transcriptional activator NifA. This protein contains three structural domains: the N-terminal domain is responsible for the negative control by fixed nitrogen; the central domain interacts with the RNA polymerase σ54 co-factor and the C-terminal domain is involved in DNA binding. The central and C-terminal domains are linked by the interdomain linker (IDL). A conserved four-cysteine motif encompassing the end of the central domain and the IDL is probably involved in the oxygen-sensitivity of NifA. In the present study,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Biological nitrogen fixation; Azospirillum brasilense; NifA protein.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200004
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Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense BJMBR
Ishida,M.L.; Assumpção,M.C.; Machado,H.B.; Benelli,E.M.; Souza,E.M.; Pedrosa,F.O..
Two Azospirillum brasilense open reading frames (ORFs) exhibited homology with the two-component NtrY/NtrX regulatory system from Azorhizobium caulinodans. These A. brasilense ORFs, located downstream to the nifR3ntrBC operon, were isolated, sequenced and characterized. The present study suggests that ORF1 and ORF2 correspond to the A. brasilense ntrY and ntrX genes, respectively. The amino acid sequences of A. brasilense NtrY and NtrX proteins showed high similarity to sensor/kinase and regulatory proteins, respectively. Analysis of lacZ transcriptional fusions by the ß-galactosidase assay in Escherichia coli ntrC mutants showed that the NtrY/NtrX proteins failed to activate transcription of the nifA promoter of A. brasilense. The ntrYX operon...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum brasilense; Two-component regulatory system; Nitrogen regulation; NtrYX genes; NtrBC genes.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600004
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Inoculação de braquiárias com Azospirillum. Infoteca-e
HUNGRIA, M.; NOGUEIRA, M. A..
bitstream/item/171535/1/folder-braquiaria.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Braquiária; Biomassa; Inoculação; Bactéria; Fertilizante nitrogenado; Azospirillum brasilense; Nitrogen fertilizers.
Ano: 2017 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1085771
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Isolation and identification by 16S rRNA sequence analysis of plant growth-promoting azospirilla from the rhizosphere of wheat BJM
Ayyaz,Khadija; Zaheer,Ahmad; Rasul,Ghulam; Mirza,Muhammad Sajjad.
ABSTRACT The main objective of the present study was to isolate phytohormone-producing, phosphate-solubilizing strains of Azospirillum from wheat to be used as inoculants for plant growth promotion. Five Azospirillum strains were isolated from the rhizosphere of field-grown wheat (Triticum aestivum L.), and it was confirmed by BOX-polymerase chain reaction (PCR) that the isolates were different and not re-isolates of the same strain. Sequence analysis of the PCR-amplified 16S rRNA gene indicated that four isolates showed maximum similarity to Azospirillum brasilense and one isolate showed maximum similarity to Azospirillum zeae. This is the first report indicating the presence of an A. zeae like isolate in the wheat rhizosphere in Pakistan. The bacterial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum brasilense; Azospirillum zeae; BOX-PCR; Indole-3-acetic acid (IAA) production; Phosphate solubilization.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300542
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O Mecanismo de regulação da FBN e sua diversidade. Infoteca-e
TEIXEIRA, K. R. dos S..
Regulação da FBN no grupo dos rhizobios. Regulação da FBN em azospirillun brasiliense. Regulação da FBN em herbaspirillun seropedicae. Regulação da FBN em acetobacter diazotrophicus.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Fixação biológica de nitrogênio; FBN; Acetobacter diazotrophicus.; Genética.; Gene; Azospirillum brasilense; Herbaspirillum seropedicae..
Ano: 1997 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/624245
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Physiological quality of corn seeds reinoculated with diazotrophic bacteria Sci. Agrar.Paran. / SAP
Sousa, Ricardo Felipe Braga; Guimarães, Vandeir Francisco; Pinto Jr., Artur Soares; Costa, Andréia Cristina Peres Rodrigues; Offemann, Luiz Claudio; Fuchs, Felipe; Coelho, Gustavo Ferreira.
This work aimed to evaluate physiological characteristics of corn seeds provenient from cultivation associated with Azospirillum brasilense and Herbaspirillum seropedicae and reinoculated with Azospirillum brasilense. The seeds were coming from cultivation in field conditions, and the treatments were: T1 - control; T2 - 60 kg hectare-1 N, T3 - inoculation (Azospirillum brasilense - AbV5); T4 - inoculation (Azospirillum brasilense) + 60 kg hectare-1 N; T5 - inoculation (Herbaspirillum seropedicae - SMR1) and T6 - inoculation (Herbaspirillum seropedicae) + 60 kg hectare-1 N. The treatments were divided in two separate blocks, being one reinoculated with A. brasilense (AbV5). The experimental design was completely randomized, in factorial scheme (6x2), being...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum brasilense; Herbaspirillum seropedicae; Seeds physiology; Reinoculation; Zea mays.
Ano: 2015 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/8753
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Plant growth-promoting bacteria associated with nitrogen fertilization at topdressing in popcorn agronomic performance Bragantia
Spolaor,Leandro Teodoski; Gonçalves,Leandro Simões Azeredo; Santos,Odair José Andrade Pais dos; Oliveira,André Luiz Martinez de; Scapim,Carlos Alberto; Bertagna,Filipe Augusto Bengosi; Kuki,Mauricio Carlos.
ABSTRACT The use of plant growth-promoting bacteria is a promising alternative with low environmental impact to increase the efficiency of use of chemical fertilizers, ensuring high yield with better cost-effective ratio. In maize crops, several studies have demonstrated an increased yield when Azospirillum-based inoculants are used. In the case of popcorn, there are no available studies related to use of inoculation and its response on yield parameters. Thus, the aim of this study was to evaluate the field performance of popcorn when inoculated with the commercial product Masterfix L (A. brasilense Ab-V5 and A. brasilense Ab-V6) and the non-commercial inoculant UEL (A. brasilense Ab-V5 + Rhizobium sp. 53GRM1) associated with nitrogen fertilization. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum brasilense; Rhizobium sp.; Inoculants; Grain yield.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000100033
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Purification and binding analysis of the nitrogen fixation regulatory NifA protein from Azospirillum brasilense BJMBR
Passaglia,L.M.P.; Van Soom,C.; Schrank,A.; Schrank,I.S..
NifA protein activates transcription of nitrogen fixation operons by the alternative sigma54 holoenzyme form of RNA polymerase. This protein binds to a well-defined upstream activator sequence (UAS) located at the -200/-100 position of nif promoters with the consensus motif TGT-N10-ACA. NifA of Azospirillum brasilense was purified in the form of a glutathione-S-transferase (GST)-NifA fusion protein and proteolytic release of GST yielded inactive and partially soluble NifA. However, the purified NifA was able to induce the production of specific anti-A. brasilense NifA-antiserum that recognized NifA from A. brasilense but not from K. pneumoniae. Both GST-NifA and NifA expressed from the E. coli tac promoter are able to activate transcription from the nifHDK...
Tipo: Info:eu-repo/semantics/article Palavras-chave: NifA protein; NifA antibodies; Azospirillum brasilense; Gel shift assay; NifA activity; Upstream activator sequence.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100001
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Sequencing and promoter analysis of the nifENXorf3orf5fdxAnifQ operon from Azospirillum brasilense Sp7 BJMBR
Potrich,D.P.; Bressel,T.A.; Schrank,I.S.; Passaglia,L.M.P..
A 40-kb DNA region containing the major cluster of nif genes has been isolated from the Azospirillum brasilense Sp7 genome. In this region three nif operons have been identified: nifHDKorf1Y, nifENXorf3orf5fdxAnifQ and orf2nifUSVorf4. The operons containing nifENX and nifUSV genes are separated from the structural nifHDKorf1Y operon by about 5 kb and 10 kb, respectively. The present study shows the sequence analysis of the 6045-bp DNA region containing the nifENX genes. The deduced amino acid sequences from the open reading frames were compared to the nif gene products of other diazotrophic bacteria and indicate the presence of seven ORFs, all reading in the same direction as that of the nifHDKorf1Y operon. Consensus sigma54 and NifA-binding sites are...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum brasilense; Nif genes; NifENX operon; Promoter activity.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100003
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Survival of endophytic diazotrophic bacteria in soil under different moisture levels BJM
Oliveira,André L.M.; Canuto,Erineudo L.; Silva,Edmilson E.; Reis,Veronica M.; Baldani,José I..
The effects of soil moisture on the survival of three diazotrophic bacteria species (Azospirillum amazonense, Gluconacetobacter diazotrophicus and Azospirillum brasilense) were tested. Soil moisture had little influence on the survival of A. brasilense, which is considered a free-living species. On the other hand, increased soil moisture extended the survival of the endophytes A. amazonense and G. diazotrophicus. These results indicate that nitrogen-fixing endophytic species are more affected by soil moisture than associative nitrogen-fixing species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Soil survival; Gluconacetobacter diazotrophicus; Azospirillum amazonense; Azospirillum brasilense.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300005
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Variabilidad genética de aislamientos no-típicos de Azospirillum brasilense por análisis PCR-RFLP del ADN 16S ribosomal Phyton
Pérez Castañeda,LM; Cruz Hernández,MA; Mendoza Herrera,A.
Diversos estudios han demostrado la asociación simbiótica de Azospirillum brasilense con diferentes gramíneas de importancia alimenticia. Así mismo, se ha encontrado una gran variabilidad entre las cepas aisladas, lo cual es consistente con la diversidad genética que Azospirillum ha mostrado en aislamientos de caña de azúcar. Sin embargo, se desconoce si esto sucede en aislados obtenidos de maíz y sorgo. Además, se ha reportado que el mayor número de aislamientos se han obtenido de regiones tropicales donde el agua no es un factor limitante. Considerando la gran diversidad que Azospirillum presenta, en este trabajo se analizó la variabilidad genética de 102 aislados de Azospirillum brasilense, provenientes de maíz y sorgo. Los trabajos, que se efectuaron...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum brasilense; Variabilidad genética; RFLP; 16S ADNr.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572011000100005
Registros recuperados: 19
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