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Amylosucrase, a glucan-synthesizing enzyme from the α-amylase family Inra
Skov, L.K.; Mirza, O.; Henriksen, A.; Potocki de Montalk, G.; Remaud-Simeon, M.; Sarçabal, P.; Willemot, R.M.; Monsan, P.; Gajhede, M..
Amylosucrase (E.C. 2.4.1.4) is a member of Family 13 of the glycoside hydrolases (the α-amylases), although its biological function is the synthesis of amylose-like polymers from sucrose. The structure of amylosucrase from Neisseria polysaccharea is divided into five domains: an all helical N-terminal domain that is not similar to any known fold, a (β/α)8-barrel A-domain, B- and B′-domains displaying α/β-structure, and a C-terminal eight-stranded β-sheet domain. In contrast to other Family 13 hydrolases that have the active site in the bottom of a large cleft, the active site of amylosucrase is at the bottom of a pocket at the molecular surface. A substrate binding site resembling the amylase 2 subsite is not found in amylosucrase. The site is blocked by a...
Tipo: Journal Article Palavras-chave: AMYLOSUCRASE; BACTERIE NON PATHOGENE; NEISSERIA POLYSACCHAREA; ALPHA AMYLASE; SACCHAROSE; STRUCTURE TRIDIMENSIONNELLE; GLYCOGENE; SEQUENCE NUCLEOTIDIQUE; ACTIVITE ENZYMATIQUE; GLYCOSIDE HYDROLYSE; NEISSERICEAE; BACTERIE GRAM NEGATIF; SUCRE.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400023316102157&uri=/notices/prodinra1/2010/10/
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Isolation and identification of the most efficient plant growth-promoting bacteria associated with canola (Brassica napus) Inra
Bertrand, H.; Nalin, R.; Bally, R.; Cleyet-Marel, J.C..
Thirteen of the most abundant Gram-negative bacteria which are able to grow in N-depleted culture conditions were isolated from the rhizoplane and endorhizosphere of canola (Brassica napus) and identified by 16S rDNA sequence analysis. Eight of these bacteria induced a significant increase in root dry weight ranging from 11 to 52%. Phylogenetic positioning based on 16S rDNA sequences indicated that at least four genera are represented, Pseudomonas, Variovorax, Agrobacterium and Phyllobacterium. The most important direct plant growth-promoting-rhizobacteria effect was found with both isolates belonging to the Phyllobacterium.
Tipo: Journal Article Palavras-chave: BRASSICA NAPUS; ASSOCIATION; BACTERIE GRAM NEGATIF; SEQUENCE NUCLEOTIDIQUE; PROMOTEUR DE CROISSANCE; RHIZOBACTERIE; PHYLLOBACTERIUM; CANOLA.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0200029473089902&uri=/notices/prodinra1/2010/12/
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Protein secretion by Gram-negative bacteria Inra
d'Enfert, C.; Reyss, I.; Wandersman, C.; Pugsley, A.P..
Tipo: Journal Article Palavras-chave: SECRETION PROTEIQUE; PROTEINE HYBRIDE; CARACTERISATION MOLECULAIRE; BACTERIE GRAM NEGATIF; OPERON.
Ano: 1989 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9200008942026535&uri=/notices/prodinra1/2010/09/
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