Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Inra (20) Autor Cossart, P. (19) Bierne, H. (3) Pizarro-Cerda, J. (3) Seveau, S. (3) Toledo-Arana, A. (3) Veiga, E. (3) Bonazzi, M. (2) Goebel, W. (2) Lecuit, M. (2) Sabet, C. (2) Tham, T. N. (2) Almo, S. C. (1) Bacher, A. (1) Batsché, E. (1) Billion, A. (1) Birmingham, C. L. (1) Boucrot, E. (1) Bougnères, L. (1) Brumell, J. H. (1) Bublitz, M. (1) Mais... Palavra-chave BACTERIE PATHOGENE (20) LISTERIA MONOCYTOGENES (15) PROTEINE (9) EXPRESSION DES GENES (6) INFECTION (6) VIRULENCE (6) ADN (4) ENZYME (4) REGULATION (4) SEQUENCE NUCLEOTIDIQUE (4) SURFACE CELLULAIRE (4) ACIDE AMINE (3) BACTERIE GRAM POSITIF (3) CADHERINE (3) HOMME (3) PROTEINE MICROBIENNE (3) SURFACE DE LA PROTEINE (3) TRANSCRIPTION (3) ACTINE (2) CELLULE HOTE (2) Mais... Tipo do documento Journal Article (20) Ano 2008 (4) 2007 (16) País France (20) Idioma Inglês (20)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 20 Primeira ... 1 ... Última A FRET analysis to unravel the role of cholesterol in Rac1 and PI 3-kinase activation in the InlB/Met signalling pathway Inra Seveau, S.; Tham, T. N.; Payrastre, B.; Hoppe, A. D.; Swanson, J. A.; Cossart, P.. The signalling pathway for the hepatocyte growth factor receptor, Met/HGF-R, is hijacked by the bacterial surface protein InlB to induce Listeria monocytogenes entry into non-phagocytic cells. We previously showed that Listeria invades host cells by interacting with specialized microdomains of the host plasma membrane called lipid rafts. In this study, we analysed in living cells signalling events that are crucial for Listeria entry using a fluorescence resonance energy transfer-based microscopic method. Phosphoinositide (PI) 3-kinase activity and Rac1 signalling induced by Listeria interacting with epithelial cells were monitored as well as signalling induced by soluble InlB and the Met natural ligand HGF. We found that InlB and HGF induced... Tipo: Journal Article Palavras-chave: PROTEINE MICROBIENNE; LISTERIA MONOCYTOGENES; BACTERIE PATHOGENE; MALADIE INFECTIEUSE; CELLULE EPITHELIALE; INTERNALINE InlB; RECEPTEUR; FACTEUR DE CROISSANCE; SIGNAL; ENZYME; CHOLESTEROL; PHOSPHOINOSITIDE KINASE. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200993335bc&uri=/notices/prodinra1/2009/09/ A naturally occurring mutation K220T in the pleiotropic activator PrfA of Listeria monocytogenes results in a loss of virulence due to decreasing DNA-binding affinity Inra Velge, P.; Herler, M.; Johansson, J.; Roche, S.; Témoin, S.; Fedorov, A. A.; Gracieux, P.; Almo, S. C.; Goebel, W.; Cossart, P.. The sequencing of prfA, encoding the transcriptional regulator of virulence genes, in 26 low virulence field Listeria monocytogenes strains showed that eight strains exhibited the same single amino-acid substitution: PrfAK220T. These strains exhibited no expression of PrfA-regulated proteins and thus no virulence. This substitution inactivated PrfA, since expression of the PrfAK220T mutant gene in an EGDΔprfA strain did not restore the haemolytic and phosphatidylcholine phospholipase C activities, in contrast to the wild-type prfA gene. The substitution of the lysine at position 220 occurred in the helix αH. However, the data showed that the PrfAK220T protein is dimerized just as well as its wild-type counterpart, but does not bind to PrfA boxes.... Tipo: Journal Article Palavras-chave: LISTERIA MONOCYTOGENES; PROTEINE; FACTEUR DE VIRULENCE; BACTERIE PATHOGENE; ADN; SEQUENCE NUCLEOTIDIQUE; REGULATION; TRANSCRIPTION; VIRULENCE; EXPRESSION DES GENES; ACIDE AMINE; MUTATION; RT-PCR; ELECTROPHORESE; ENZYME; ARN POLYMERASE. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009c1895b3c&uri=/notices/prodinra1/2009/11/ An important step in Listeria lipoprotein research Inra Garcia-del Portillo, F.; Cossart, P.. Over the last 10 years, DNA sequences of more than 600 bacterial species have been deposited in databases and are now available to search any gene, motif, or regulatory sequence of interest. Although genome data are instrumental in phylogenetic analysis and in silico design of metabolic and regulatory networks, only a very small fraction of the information has been experimentally validated. A striking example is lipoproteins predicted from genome sequences. Despite the predominance of this class of surface proteins in bacteria (up to 0.5 to 8% of the proteome), very few of these proteins have been identified as lipoproteins by biochemical methods (19). In this issue of the Journal of Bacteriology, Baumgärtner et al. (2) report a systematic... Tipo: Journal Article Palavras-chave: LIPOPROTEINE; LISTERIA MONOCYTOGENES; VIRULENCE; BACTERIE GRAM-POSITIVE; BACTERIE GRAM-NEGATIVE; BACTERIE PATHOGENE; ADN; SEQUENCE NUCLEOTIDIQUE; ANALYSE PHYLOGENETIQUE; REGULATION; PROTEINE; SURFACE CELLULAIRE; VIRULENCE; TECHNIQUE ANALYTIQUE; BIOCHIMIE. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009594c400a&uri=/notices/prodinra1/2009/11/ Crystal structure and standardized geometric analysis of InlJ, a listerial virulence factor and leucine-rich repeat protein with a novel cysteine ladder Inra Bublitz, M.; Holland, C.; Sabet, C.; Reichelt, J.; Cossart, P.; Heinz, D. W.; Bierne, H.; Schubert, W. D.. We report on the crystal structure of the internalin domain of InlJ, a virulence-associated surface protein of Listeria monocytogenes, at 2.7-Å resolution. InlJ is a member of the internalin family of listerial cell surface proteins characterized by a common N-terminal domain. InlJ bears 15 leucine-rich repeats (LRRs), the same number as in InlA, the prototypical internalin family member. The LRRs of InlJ differ from those of other internalins by having 21, rather than 22, residues and by replacing 1 LRR defining hydrophobic residue with a conserved cysteine. These cysteines stack to form an intramolecular ladder and regular hydrophobic interactions in consecutive repeats. Analyzing the curvature, twist, and lateral bending angles of InlJ and... Tipo: Journal Article Palavras-chave: ACIDE AMINE; LEUCINE; CYSTEINE; PROTEINE; LISTERIA MONOCYTOGENES; BACTERIE PATHOGENE; VIRULENCE; SURFACE DE LA PROTEINE; SEQUENCE NUCLEOTIDIQUE LEUCINE-RICH REPEAT; CYSTEINE LADDER; ASPARAGINE LADDER; INTERNALIN; REPEAT GEOMETRY. Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009da5e3652&uri=/notices/prodinra1/2010/11/ Histone modifications induced by a family of bacterial toxins Inra Hamon, M. A.; Batsché, E.; Régnault, B.; Tham, T. N.; Seveau, S.; Muchardt, C.; Cossart, P.. Upon infection, pathogens reprogram host gene expression. In eukaryotic cells, genetic reprogramming is induced by the concerted activation/repression of transcription factors and various histone modifications that control DNA accessibility in chromatin. We report here that the bacterial pathogen Listeria monocytogenes induces a dramatic dephosphorylation of histone H3 as well as a deacetylation of histone H4 during early phases of infection. This effect is mediated by the major listerial toxin listeriolysin O in a pore-forming-independent manner. Strikingly, a similar effect also is observed with other toxins of the same family, such as Clostridium perfringens perfringolysin and Streptococcus pneumoniae pneumolysin. The decreased levels of... Tipo: Journal Article Palavras-chave: HISTONE; BACTERIE PATHOGENE; AGENT PATHOGENE; EXPRESSION DES GENES; ADN; SEQUENCE NUCLEOTIDIQUE; INFECTION; TRANSCRIPTION; REGULATION; EUCARYOTE; PHOSPHORE; DEPHOSPHORYLATION EPIGENETIC; LISTERIA MONOCYTOGENES; PATHOGENESIS . Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009b0bc6f33&uri=/notices/prodinra1/2009/11/ Human listeriosis and animal models Inra Lecuit, M.. Human listeriosis is a potentially fatal foodborne infection caused by Listeria monocytogenes, an opportunistic psychrophile bacterium that is widespread in the environment. It has only recently emerged as a significant cause of human infection in industrialized countries, owing to appearance of a vulnerable population of immunocompromised individuals, and the concomitant development of large-scale agro-industrial plants and refrigerated food. Here we review the main clinical features of human listeriosis and highlight specificities and similarities with animal listeriosis in diverse species. Finally, we present some of the critical determinants for the choice of an appropriate animal model to study human listeriosis. Tipo: Journal Article Palavras-chave: LISTERIOSE; BACTERIE PATHOGENE; MODELE ANIMAL; INFECTION; HOMME; MALADIE; IMMUNITE LISTERIOSIS; LISTERIA MONOCYTOGENES; ANIMAL MODEL . Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009e8130595&uri=/notices/prodinra1/2009/11/ Identification of new noncoding RNAs in Listeria monocytogenes and prediction of mRNA targets Inra Mandin, P.; Repoila, F.; Vergassola, M.; Geissmann, T.; Cossart, P.. To identify noncoding RNAs (ncRNAs) in the pathogenic bacterium Listeria monocytogenes, we analyzed the intergenic regions (IGRs) of strain EGD-e by in silico-based approaches. Among the twelve ncRNAs found, nine are novel and specific to the Listeria genus, and two of these ncRNAs are expressed in a growth-dependent manner. Three of the ncRNAs are transcribed in opposite direction to overlapping open reading frames (ORFs), suggesting that they act as antisense on the corresponding mRNAs. The other ncRNA genes appear as single transcription units. One of them displays five repeats of 29 nucleotides. Five of these new ncRNAs are absent from the non-pathogenic species L. innocua, raising the possibility that they might be involved in... Tipo: Journal Article Palavras-chave: BACTERIE PATHOGENE; PROTEINE BACTERIENNE; LISTERIA MONOCYTOGENES; VIRULENCE; ARN; ARN MESSAGER; TRANSCRIPTION; TECHNIQUE ANALYTIQUE; SEQUENCE NUCLEOTIDIQUE; GENE ANTISENS; VIRULENCE. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009f388695&uri=/notices/prodinra1/2009/11/ Internalins: a complex family of leucine-rich repeat-containing proteins in Listeria monocytogenes Inra Bierne, H.; Sabet, C.; Personnic, N.; Cossart, P.. The Listeria monocytogenes genome includes a large family of proteins harbouring leucine-rich repeats known as internalins (Inl). The generation of novel mutants and comparative analysis of Inl variability among Listeria and other bacterial genomes suggest that beyond the extensively-studied invasins, InlA and InlB, additional internalins also play important functions in the infectious process. Tipo: Journal Article Palavras-chave: LISTERIA MONOCYTOGENES; MICROORGANISME; BACTERIE PATHOGENE; SURFACE DE LA PROTEINE; LEUCINE; ACIDE AMINE; PROTEINE; MUTATION; EXPRESSION DES GENES; INTERNALINE; INFECTION; POUVOIR PATHOGENE LEUCINE-RICH REPEAT; PATHOGENICITY; SURFACE PROTEIN; VIRULENCE FACTORS; INVASIN. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20095b9ab581&uri=/notices/prodinra1/2010/11/ Invasive and adherent bacterial pathogens co-opt host clathrin for infection Inra Veiga, E.; Guttman, J. A.; Bonazzi, M.; Boucrot, E.; Toledo-Arana, A.; Lin, A. E.; Enninga, J.; Pizarro-Cerda, J.; Finlay, B. B.; Kirchhausen, T.; Cossart, P.. Infection by the bacterium Listeria monocytogenes depends on host cell clathrin. To determine whether this requirement is widespread, we analyzed infection models using diverse bacteria. We demonstrated that bacteria that enter cells following binding to cellular receptors (termed ‘‘zippering’’ bacteria) invade in a clathrin-dependent manner. In contrast, bacteria that inject effector proteins into host cells in order to gain entry (termed ‘‘triggering’’ bacteria) invade in a clathrin-independent manner. Strikingly, enteropathogenic Escherichia coli (EPEC) required clathrin to form actin-rich pedestals in host cells beneath adhering bacteria, even though this pathogen remains extracellular. Furthermore, clathrin accumulation preceded the actin... Tipo: Journal Article Palavras-chave: BACTERIE PATHOGENE; LISTERIA MONOCYTOGENE; INFECTION BACTERIENNE; PROTEINE; CLATHRINE; ESCHERICHIA COLI; INFECTION; MODELE; ADHERENCE. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20094c6cd329&uri=/notices/prodinra1/2009/09/ Listeria comet tails: the actin-based motility machinery at work Inra Lambrechts, A.; Gevaert, K.; Cossart, P.; Vandekerckhove, J.; van Troys, M.. Listeria monocytogenes is a master of mimicry that uses the host cell actin system both to move within the cytoplasm of infected cells and for cell-to-cell spread. Recent studies of Listeria and similarly acting pathogens have generated leaps in our understanding of the actinbased force producing machinery. This machinery is essential for most motile properties of cells, not least for cell migration. In a minimal configuration, it consists of the Arp2/3-complex, Ena–VASP proteins, cofilin, capping protein and a nucleation-promoting factor. In this review, we discuss current models of pseudopodial protrusions and describe how the road to more complex models lies open and is already paved by recent studies using Listeria-based biomimetic... Tipo: Journal Article Palavras-chave: LISTERIA MONOCYTOGENES; MOTILITE; ACTINE; PROTEINE; BACTERIE PATHOGENE; INFECTION; CYTOPLASME; POUVOIR PATHOGENE; ACTINE; MODELISATION; PROTEINE; EXPRESSION DES GENES. Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200990b263f3&uri=/notices/prodinra1/2010/11/ Listeria InlB takes a different route to met Inra Veiga, E.; Cossart, P.. InlB, a surface protein of the human bacterial pathogen Listeria monocytogenes, interacts with the receptor tyrosine kinase Met on host cells to enable bacterial invasion. In this issue, Niemann et al. (2007) provide the first structural evidence that InlB does not compete for the same interaction site on Met as the natural ligand HGF. Tipo: Journal Article Palavras-chave: BACTERIE PATHOGENE; LISTERIA MONOCYTOGENES; PROTEINE; RECEPTEUR; TYROSINE KINASE; HOMME; SURFACE CELLULAIRE. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009a8e51aff&uri=/notices/prodinra1/2009/09/ Listeria monocytogenes, a unique model in infection biology: an overview Inra Cossart, P.; Toledo-Arana, A.. This review rather than covering the whole field intends to highlight recent findings on the Listeria monocytogenes infectious process or some Listeria specific traits, place them within the framework of well-established data, and demonstrate how this Gram-positive bacterium has, in two decades, emerged as a multifaceted paradigm. Indeed, the cell biology of the infectious process has been deciphered in great detail and provided insights in both the way bacterial pathogen manipulate the host and unsuspected functions of well-known cellular proteins. The intra- and intercellular motility has in particular been instrumental in understanding actin-based motility in general. The analysis of the two main Listeria invasion proteins and that of their host... Tipo: Journal Article Palavras-chave: LISTERIA MONOCYTOGENES; BACTERIE PATHOGENE; CLATHRINE; CADHERINE; ARNs; BACTERIE GRAM POSITIF; IMMUNOLOGIE; PROTEINE; VIRULENCE CLATHRIN; CYTOSKELETON; SMALL RNAs; THERMOSENSOR; HOST BARRIER; MET; E-CADHERIN. Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20098e2ea7db&uri=/notices/prodinra1/2009/12/ Listeria monocytogenes evades killing by autophagy during colonization of host cells Inra Birmingham, C. L.; Canadien, V.; Gouin, E.; Troy, E. B.; Yoshimori, T.; Cossart, P.; Higgins, D.; Brumell, J. H.. Listeria monocytogenes is an intracellular pathogen that is able to colonize the cytosol of macrophages. Here we examined the interaction of this pathogen with autophagy, a host cytosolic degradative pathway that constitutes an important component of innate immunity towards microbial invaders. L. monocytogenes infection induced activation of the autophagy system in macrophages. At 1 h post infection (p.i.), a population of intracellular bacteria (~37%) colocalized with the autophagy marker LC3. These bacteria were within vacuoles and were targeted by autophagy in an LLO-dependent manner. At later stages in infection (by 4 h p.i.), the majority of L. monocytogenes escaped into the cytosol and rapidly replicated. At these times, less than 10% of... Tipo: Journal Article Palavras-chave: LISTERIA MONOCYTOGENES; PATHOGENE INTRACELLULAIRE; BACTERIE PATHOGENE; INFECTION; MACROPHAGE; AUTOPHAGE; CYTOSOL; POUVOIR PATHOGENE; VIRULENCE; PHOSPHOLIPASE; ENZYME; BACTERIE GRAM POSITIF. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20096e981c45&uri=/notices/prodinra1/2009/09/ Listeria monocytogenes surface proteins: from genome predictions to function Inra Bierne, H.; Cossart, P.. Tipo: Journal Article Palavras-chave: LISTERIA MONOCYTOGENES; PROTEINE BACTERIENNE; PENETRATION CELLULAIRE; ENVELOPPE CELLULAIRE; SURFACE DE LA PROTEINE; PREDICTION; BACTERIE PATHOGENE; PROTEINE; PAROI CELLULAIRE; ENZYME; CELLULE HOTE; ADHESION. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009bd3d54c7&uri=/notices/prodinra1/2009/11/ Listeriology (1926-2007): the rise of a model pathogen Inra Cossart, P.. Listeria monocytogenes is an ubiquitous gram positive bacterium responsible for a severe food borne disease in human and animals which has become a multifaceted model. Indeed, it is a facultative intracellular bacterium that induces an amazingly rapid and sterilizing T-cell response and has been - and still is - widely used by immunologists. Listeria’s strategies to invade non-phagocytic cells and spread from cell to cell have been and still are outstandingly instrumental to address key questions in cell biology. Orally acquired listeriosis can now be studied in relevant animal models. Finally, Listeria is among the few bacteria which have so rapidly benefited from comparative genomics. It now ranks among the most documented pathogens. Tipo: Journal Article Palavras-chave: BACTERIE PATHOGENE; MICROBIOLOGIE CELLULAIRE; ACTINE; MODELE TRANSGENIQUE; BACTERIE GRAM POSITIF; MODELE ANIMAL LISTERIA; PATHOGENESIS; CELLULAR MICROBIOLOGY; ACTIN; TRANSGENIC MODEL. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20097c9f229d&uri=/notices/prodinra1/2009/09/ Molecular mechanisms exploited by Listeria monocytogenes during host cell invasion Inra Seveau, S.; Pizarro-Cerda, J.; Cossart, P.. The facultative intracellular bacterial pathogen Listeria monocytogenes has evolved multiple strategies to invade a large panel of mammalian cells. Host cell invasion is critical for several stages of listeriosis pathology such as the initial crossing of the host intestinal barrier and the successive colonization of diverse target organs including the placenta. In this review, we address the main molecular mechanisms known to be used by L. monocytogenes during invasion of nonphagocytic cells and host tissues. Tipo: Journal Article Palavras-chave: BACTERIE PATHOGENE; PHAGOCYTOSE; INVASION; CELLULE HOTE; LISTERIOSE LISTERIA MONOCYTOGENES; MAMMALIAN CELL; PLACENTA; HOST TISSUE; NONPHAGOCYTIC CELL. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009c57b874d&uri=/notices/prodinra1/2009/11/ Pathogenomics of Listeria spp. Inra Hain, T.; Chatterjee, S. S.; Ghai, R.; Kuenne, C. T.; Billion, A.; Steinweg, C.; Domann, E.; Kärst, U.; Jänsch, L.; Wehland, J.; Eisenreich, W.; Bacher, A.; Joseph, B.; Schär, J.; Kreft, J.; Klumpp, J.; Loessner, M. J.; Dorscht, J.; Neuhaus, K.; Fuchs, T. M.; Scherer, S.; Doumith, M.; Jacquet, C.; Martin, P.; Cossart, P.; Rusniock, C.; Glaser, P.; Buchrieser, C.; Goebel, W.; Chakraborty, T.. This review provides an overview of recent progress in the exploration of genomic, transcriptomic, and proteomic data in Listeria spp. to understand genome evolution and diversity, as well as physiological aspects of metabolism utilized by the bacteria when growing in diverse and varied environments. Tipo: Journal Article Palavras-chave: LISTERIA; BACTERIE; GENOME; ADN; GENOMIQUE; TRANSCRIPTOMIQUE; PROTEOMIQUE; METABOLISME; IMPACT DE L'ENVIRONNEMENT; DIVERSITE; EXPRESSION DES GENES; BACTERIE PATHOGENE; PATHOGENICITE; VARIABILITE LISTERIA; GENOMIC; TRANSCRIPTOMIC; PROTEOMIC; BACTERIOPHAGE; METABOLISM. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20097b41fd4e&uri=/notices/prodinra1/2009/12/ Small noncoding RNAs controlling pathogenesis Inra Toledo-Arana, A.; Cossart, P.. Infectious diseases are a leading cause of mortality worldwide. A major challenge in achieving their eradication is a better understanding of bacterial pathogenesis processes. The recent discovery of small noncoding RNAs (sRNAs) as modulators of gene expression in response to environmental cues has brought a new insight into bacterial regulation. sRNAs coordinate complex networks of stress adaptation and virulence gene expression. sRNAs generally ensure such a regulation by pairing to mRNAs of effector and/or regulatory genes, or by binding to proteins. An updated view on bacterial models responsible for important infections illustrates the key role of sRNAs in the control of pathogenesis. Tipo: Journal Article Palavras-chave: MALADIE INFECTIEUSE; BACTERIE PATHOGENE; ARNS; GENE; FACTEUR DE VIRULENCE; PATHOGENESE; BACTERIE PATHOGENE; EXPRESSION DES GENES; REGULATION; ADAPTATION AU STRESS; VIRULENCE. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009d5b2a802&uri=/notices/prodinra1/2010/11/ Src, cortactin and Arp2/3 complex are required for E-cadherin-mediated internalization of Listeria into cells Inra Sousa, S.; Cabanes, D.; Bougnères, L.; Lecuit, M.; Sansonetti, P.; Tran-Van-Nhieu, G.; Cossart, P.. Listeria monocytogenes is a food-borne pathogen able to invade non-phagocytic cells. InlA, a L. monocytogenes surface protein, interacts with human E-cadherin to promote bacterial entry. L. monocytogenes internalization is a dynamic process involving co-ordinated actin cytoskeleton rearrangements and host cell membrane remodelling at the site of bacterial attachment. Interaction between E-cadherin and catenins is required to promote Listeria entry, and for the establishment of adherens junctions in epithelial cells. Although several molecular factors promoting E-cadherin-mediated Listeria internalization have been identified, the proteins regulating the transient actin polymerization required at the bacterial entry site are unknown. Here we... Tipo: Journal Article Palavras-chave: PROTEINE MICROBIENNE; LISTERIA MONOCYTOGENES; BACTERIE PATHOGENE; CADHERINE; PENETRATION CELLULAIRE; SURFACE CELLULAIRE; HOMME; MODELISATION; MEMBRANE CELLULAIRE. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20092f54e3ca&uri=/notices/prodinra1/2009/09/ Successive post-translational modifications of E-cadherin are required for InlA-mediated internalization of Listeria monocytogenes Inra Bonazzi, M.; Veiga, E.; Pizarro-Cerda, J.; Cossart, P.. Listeria monocytogenes surface proteins internalin (Inl)A and InlB interact with the junctional protein E-cadherin and the hepatocyte growth factor (HGF) receptor Met, respectively, on the surface of epithelial cells to mediate bacterial entry. Here we show that InlA triggers two successive E-cadherin posttranslationalmodifications, i.e. the Src-mediatedtyrosine phosphorylation of E-cadherin followed by its ubiquitination by the ubiquitin-ligase Hakai. E-cadherin ubiquitination induces the recruitment ofclathrin that is required for optimal bacterial internalization. We also show that the initial clustering of E-cadherin at the bacterial entry site requirescaveolin, a protein normally involved in clathrinindependent endocytosis. Strikingly... Tipo: Journal Article Palavras-chave: BACTERIE PATHOGENE; LISTERIA MONOCYTOGENES; PROTEINE MICROBIENNE; CADHERINE; SURFACE CELLULAIRE; GLYCOPROTEINE; FACTEUR DE CROISSANCE; ENDOCYTOSE E-CADHERIN. Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009b5349ff4&uri=/notices/prodinra1/2009/09/ Registros recuperados: 20 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco