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| Registros recuperados: 10 | |
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| Furuoka, Hidefumi; Yabuzoe, Atushi; Horiuchi, Motohiro; Tagawa, Yuichi; Yokoyama, Takashi; Yamakawa, Yoshio; Shinagawa, Morikazu; Sata, Tetsutaro. |
| For immunohistochemistry of the prion diseases, several pretreatment methods to enhance the immunoreactivity of human and animal abnormal proteinase-resistant prion protein (PrPSc) on the tissue sections have been employed. The method of 121 degrees C hydrated autoclaving pretreatment or the combination method of 121 degrees C hydrated autoclaving with a certain chemical reagent ( formic acid or proteinase K, etc) are now widely used. We found that an improved hydrated autoclaving method at 135 degrees C, more effectively enhanced PrPSc immunoreactivity for the antibodies recognizing the linear epitope. In addition, this method was more effective for the long-term fixation samples as compared with other previous methods. However, this modified method could... |
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Palavras-chave: Prion protein; Immunohistochemistry; Antigen retrieval; Autoclaving; Monoclonal antibody; CREUTZFELDT-JAKOB-DISEASE; MONOCLONAL-ANTIBODIES; IMMUNOCYTOCHEMISTRY; IMMUNOREACTIVITY; PROTOCOLS; FIXATION; DEPOSITS; PLAQUES; BRAIN; PRP. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/712 |
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| Trujillo-Cenóz, O.; Melamed, J.. |
| The pedunculate bodies consist of three fibrillar regions: calyces, stalk, and roots. A group of small association neurons (the globule cells) lies within and around the calyces. The synaptic region of the calyces shows, under the phase contrast microscope, small, dense circumscribed areas of neuropil corresponding to the so-called “glomeruli” of light microscopists. The electron microscope demonstrated that each glomerulus consists of a large central nerve ending and several thin peripheral fibers. The central ending is filled with small vesicles. Mitochondria and dense granules are found intermixed with the vesicular material. The peripheral fibers contain mitochondria, slender tubules, and a few vesicles. The origin of the central ending and... |
| Tipo: Journal Article-postprint |
Palavras-chave: INSECT; BRAIN; NEURONE; PEDUNCULATE BODY; CALYCE; ELECTRON MICROSCOPE; INSECTE; CERVEAU. |
| Ano: 1962 |
URL: http://hdl.handle.net/2174/513 |
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| André, A.; Juanéda, P.; Sébédio, J.L.; Chardigny, J.M.. |
| Plasmalogens (Pls) are phospholipids containing a vinyl–ether bond at the sn-1 position of the glycerol backbone. They represent between 1/2 and 2/3 of the ethanolamine phospholipids in the brain. During aging, the Pls content in human brain falls down. However, the role of Pls metabolism-related enzymes in the regulation of Pls levels remains to be determined. Dihydroxyacetone phosphate acyltransferase (DHAP-AT) is the enzyme involved in the first step of Pls biosynthesis. In the brain, a phospholipase A2, which selectively acts on Pls, has been isolated (Pls-PLA2s). In this work, we aimed to evaluate the impact of DHAP-AT (a key enzyme of Pls biosynthesis) and Pls-PLA2 (a specific Pls degradation enzyme) on the evolution of Pls content in the rat brain... |
| Tipo: Journal Article |
Palavras-chave: PLASMALOGEN; DIHYDROXYACETONE PHOSPHATE ACYLTRANSFERASE; PHOSPHOLIPASE A2; AGING; BRAIN. |
| Ano: 2006 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008a0cc61d6&uri=/notices/prodinra1/2008/11/ |
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| Kollmann, M.; Minoli, S.; Bonhomme, J.; Homberg, J.; Schachtner, J.; Tagu, D.; Anton, S.. |
| Aphids show a marked phenotypic plasticity, producing asexual or sexual and winged or wingless morphs depending on environmental conditions and season. We describe here the general structure of the brain of various morphs of the pea aphid Acyrthosiphon pisum. This is the first detailed anatomical study of the central nervous system of an aphid by immunocytochemistry (synapsin, serotonin, and several neuropeptides), ethyl-gallate staining, confocal laser scanning microscopy, and three-dimensional reconstructions. The study has revealed well-developed optic lobes composed of lamina, medulla, and lobula complex. Ocelli are only present in males and winged parthenogenetic females. The central complex is well-defined, with a central body divided into two parts,... |
| Tipo: Journal Article |
Palavras-chave: PUCERON; PLASTICITE PHENOTYPIQUE; CERVEAU INSECT NERVOUS SYSTEM; BRAIN; NEUROPIL; PHENOTYPIC PLASTICITY; APHIDS. |
| Ano: 2011 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011eb8844e3&uri=/notices/prodinra1/2011/03/ |
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| Registros recuperados: 10 | |
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