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Registros recuperados: 26 | |
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Wu,Gaobing; Zhan,Tao; Guo,Yiming; Kumar,Ashok; Liu,Ziduo. |
Background: Glycine oxidase (GO), a type of D-amino acid oxidase, is of biotechnological interest for its potential in several fields. In our previous study, we have characterized a new glycine oxidase (BceGO) from Bacillus cereus HYC-7. Here, a variant of N336K with increased the affinity against all the tested substrate was obtained by screening a random mutant library of BceGO. It is observed that the residue N336 is invariable between its homogeneous enzymes. This work was aimed to explore the role of the residue N336 in glycine oxidase by site-directed mutagenesis, kinetic assay, structure modeling and substrate docking. Results: The results showed that the affinity of N336H, N336K and N336R increased gradually toward all the substrates, with increase... |
Tipo: Journal article |
Palavras-chave: Bacillus cereus; Error-prone PCR; Glycine oxidase; Site-directed mutagenesis; Substrate affinity. |
Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400004 |
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BANERJEE,GOUTAM; GORTHI,SRIKANTH; CHATTOPADHYAY,PRITAM. |
ABSTRACT Disease in agricultural field is a big problem that causes a massive loss in production. In this present investigation, we have reported a soil-borne bacterium Bacillus cereus IB311 which is antagonistic to plant pathogens (Pseudomonas syringae and Agrobacterium tumefaciens), and could make a substantial contribution to the prevention of plant diseases. To prove the practical application, the strain was directly applied in agricultural field. The results demonstrated that B. cereus IB311 has increased the production (20% and 26% in term of average pod number per plant, average seed number per pod, and seed yield per experimental plot) in ground nut (Arachis hypogaea var. Koushal, G201) and sesame (Sesamum indicum var. Kanak), respectively. To... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus cereus; Bio-control; Phytopathogens; RSM; Field application; Crop production. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502149 |
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Garcia,Flávio Augusto de Oliveira; Romeiro,Reginaldo da Silva. |
O objetivo deste trabalho foi avaliar o efeito de isolados de Bacillus cereus e Pseudomonas putida sobre a severidade da mancha-angular do feijoeiro, em condições de campo. Quatro experimentos foram conduzidos em épocas favoráveis à mancha-angular (Pseudocercospora griseola), com as cultivares Pérola e Ouro Negro. Os experimentos foram realizados em delineamento de blocos ao acaso, com seis repetições. Os tratamentos foram pulverizações sobre as plantas com três estirpes de B. cereus (UFV-172, UFV-101 e UFV-75) e duas estirpes de P. putida (UFV-53 e UFV-Pp), acibenzolar-S-metil, fungicida clorotalonil e controle pulverizado com água, no total de oito tratamentos. Os tratamentos foram aplicados por atomização no filoplano de plantas de feijoeiro,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus cereus; Phaseolus vulgaris; Pseudocercospora griseola; Pseudomonas putida. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001200004 |
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Heling, Anderson Luis; Kuhn, Odair José; Stangarlin, José Renato. |
The Mycosphaerella spot is one of the main foliar diseases of strawberry, degrating great leaf regions and reducing the photosynthetic area. Its control is mainly by the use of chemical fungicides, but, due the increasing demand for food free of pesticide, alternative control methods have been researched, such as biological control. This work aimed to evaluate the effect on strawberry plants, treated with the biological control agents Bacillus cereus, Saccharomyces boulardii and Saccharomyces cerevisiae, in the severity of Mycosphaerella fragariae, productivity and in the activity of β-1.3 glucanases, peroxidases and chitinases enzymes. It was verified that S. cerevisiae and B. cereus treatments were similar to fungicide for disease control. However, even... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Controle alternativo; Bacillus cereus; Saccharomyces boulardii; Saccharomyces cerevisiae. |
Ano: 2015 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/9544 |
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Sánchez,Lilian; Tromps,Jeannette. |
Las propiedades beneficiosas de las bacterias ácido lácticas (BAL) posibilitan su uso como probióticas en animales y humanos. El objetivo del presente trabajo fue caracterizar, mediante pruebas in vitro, cepas BAL procedentes de fuente animal para considerar su posterior aplicación como probiótico en animales. Se evaluaron 72 cepas, identificadas hasta el nivel de género, de Lactobacillus spp., Leuconostoc spp., Lactococcus spp., Streptococcus spp. y Pediococcus spp. A todas las cepas se les determinó su estabilidad de crecimiento a diferentes pH, temperatura y tolerancia a altas concentraciones de cloruro de sodio. De ellas se seleccionaron 14 cepas que presentaron las mejores características como candidatos a probióticas por sus resultados a las pruebas... |
Tipo: Journal article |
Palavras-chave: Bacterias ácido-láctica; Probióticos; Bacillus cereus; Staphylococcus aureus; Salmonella spp.; Pseudomonas aeruginosa. |
Ano: 2014 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000200008 |
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Corrêa,Élida Barbosa; Galvão,José Abrahão Haddad; Bettiol,Wagner. |
O objetivo deste trabalho foi avaliar a capacidade de microrganismos de manguezais para controlar a podridão radicular causada por Pythium aphanidermatum e para promover o crescimento em pepino hidropônico (Cucumis sativus). Avaliaram-se 19 microrganismos quanto ao controle da doença em mini-hidroponia. Os microrganismos mais promissores para esse fim - Gordonia rubripertincta SO-3B-2 e a mistura dos isolados G. rubripertincta SO-3B-2, MB-P3A-49, MB-P3-C68 e SO-3L-3, de Pseudomonas stutzeri, e Bacillus cereus AVIC-3-6 - foram, posteriormente, testados quanto à promoção de crescimento do pepineiro, em casa de vegetação. Microrganismos de manguezais podem ter importância funcional no controle biológico da podridão radicular causada por P. aphanidermatum e na... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus cereus; Cucumis sativus; Gordonia rubripertincta; Pseudomonas stutzeri; Pythium aphanidermatum; Bioprospecção. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000200003 |
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Salustiano,V.C; Andrade,N.J; Ribeiro Junior,J.I; Fernandes,P.E; Lopes,J.P; Bernardes,P.C; Portugal,J.G. |
Bacillus cereus adherence to stainless steel used to milk contact surfaces was observed, depending on the cleaning and sanitizing procedures applied and the physicochemical properties of the surfaces. Numbers of surviving B. cereus after hygiene procedures were affected by temperature, the concentrations of both alkaline and acid washes, and the pH of the chlorine solution. The adhesion of B. cereus to the stainless steel was not thermodynamically favorable, and the adherence of this microorganism occurred in lower number, in accordance to the thermodynamic aspect of adhesion. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus cereus; Equipment surface; Dairy industry; Hygiene procedures. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600026 |
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Vidal,Ana Maria Centola; Rossi Junior,Oswaldo Durival; Abreu,Irlan Leite de; Bürger,Karina Paes; Cardoso,Marita Vedovelli; Gonçalves,Ana Carolina Siqueira; Rossi,Gabriel Augusto Marques; D'Abreu,Léa Furlan. |
ABSTRACT: The present study focused on isolation Bacillus cereus during the UHT milk production and shelf life, to assess the enterotoxigenic production capacity of isolates and to evaluate the use of the RAPD-PCR technique to verify whether Bacillus cereus isolated at different phases of UHT milk processing belongs to the same strain. For this, six groups of milk samples composed of raw, pasteurized and UHT milk were collected from a processing plant. The results revealed that bacteria belonging to the Bacillus cereus group were isolated from 51.6%, 81.6% and from 13.8% of raw, pasteurized and UHT milk samples, respectively. About 50.0% of isolates from raw milk, 19.2% isolates from pasteurized milk and 70.7% isolates from UHT milk were capable of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus cereus; Enterotoxins; Microbiological quality; Pasteurized milk; Raw milk; UHT milk. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200286 |
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Fritz,Leila Lucia; Berlitz,Diouneia Lisiane; Macedo,Vera Regina Mussoi; Machado,Vilmar; Fiuza,Lidia Mariana. |
Bacilos entomopatogênicos são bactérias observadas principalmente no solo, consideradas importantes agentes no controle biológico de doenças e pragas da agricultura. No entanto, existem poucos estudos que relatam a influência das práticas agrícolas nas populações de Bacillus spp. Essa pesquisa objetivou avaliar a freqüência de bactérias entomopatogênicas pertencentes ao gênero Bacillus obtidas de amostras de solo coletadas no ano agrícola de 2006/2007 de diferentes sistemas de cultivo de arroz irrigado da EEA-IRGA, Cachoeirinha (RS). Foram analisadas 36 amostras de solos, oriundas de três sistemas de plantio de arroz irrigado, pré-germinado (SPG), convencional (SPC), plantio direto (SPD) e de uma área sem plantio de arroz (C). Para tanto, um grama de solo... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agroecossistemas; Bacilos entomopatogênicos; Sistemas de plantio; Bacillus thuringiensis; Bacillus sphaericus; Bacillus cereus. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052010000200020 |
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Reis,Andre L.S.; Montanhini,Maike T.M.; Bittencourt,Juliana V.M.; Destro,Maria T.; Bersot,Luciano S.. |
Bacillus cereusis an ubiquitous, spore-forming bacteria that can survive pasteurization and the majority of the heating processes used in the dairy industry. Besides, it is a pathogen responsible for different types of food poisoning. One type of foodborne disease caused by B.cereusis the diarrheal syndrome, which is caused by the ingestion of vegetative cells producing toxins in the small intestine. One virulence factor for the diarrheal syndrome is the toxin hemolysin BL (HBL), a three-component protein formed by the L1, L2 and B components. In order to evaluate the presence of diarrheal strains isolated from milk and dairy products, 63 B. cereus isolates were obtained from 260 samples of UHT milk, pasteurized milk and powdered milk, sold in commercial... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Milk; Dairy products; Bacillus cereus; Pathogenicity; Detection. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400024 |
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Iurkiv, Luciana; Dildey, Omari Dangelo Forlin; Coltro-Roncato, Sidiane; Gonçalves-Trevisoli, Edilaine Della Valentina; Rissato, Bruna Broti; Guimarães, Vandeir Francisco; Stangarlin, José Renato. |
The induced resistance eficiency in the control of phytopathogens is recognized, however little is known about its interference on non-target microrganisms. The present study aims to verify the induction of peroxidase activity by the use of acibenzolar-S-methyl (ASM), mannanoligosaccharide (MOS) and Bacillus cereus inductor in the control of spot blotch wheat disease and its interference on the non-target microrganism A. brasilense. The experiment was conduced under green house growing. The experimental design was made in randomized block design in a factorial schema 4 x 2 x 2, with 4 replications. The first factor "Resistance Inductors" was constituted by ASM, MOS, B. cereus inductors and water (control). The second factor "Pathogen" was constituted by... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acibenzolar-S-metil; Bacillus cereus; Indução de resistência; Mananoligossacarídeo fosforilado; Mancha marrom.. |
Ano: 2017 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/14817 |
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Heling, A. L.; Favorito, P. A.; Kuhn, O. J.. |
The strawberry is a fruit of great market acceptance and it is much appreciated throughout the world, besides being very important for small farmers, generating employment and income. Nevertheless, this crop is affected by various diseases that require control, so that producers eventually use chemical fungicides, which, besides contaminating soil and water, eventually contaminate the fruit. In an attempt to reduce the amount of pesticides used in the strawberry crop, a lot of research on biological control of diseases has been done. However, the biological control of diseases in strawberries should not interfere negatively in fruit quality. Thus, this study aimed to evaluate the acidity, pH and total soluble solids (ºBrix) of strawberries treated with... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus cereus; Controle biológico; Saccharomyces boulardii; Saccharomyces cerevisiae. |
Ano: 2013 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/7869 |
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Registros recuperados: 26 | |
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