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	Provedor de dados 52 (4) 37 (1) Autor Nascimento,Aguinaldo José do (3) Higuti,Ilma Hiroko (2) Silva,Priscila Anunciação da (2) Andrade,Edicléia Alves de (1) Binod,Parameswaran (1) CORTÉS PÉREZ,Francisco (1) Grande,Simone Wichert (1) Higuti,lma Hiroko (1) LIZARAZO FORERO,Luz Marina (1) Marcondes,Aluizio de Abreu (1) Okiyama,Vivian Mayumi de Eiróz (1) Pandey,Ashok (1) Papp,Juliana (1) Sacco,Roberta (1) Shalin,Thomas (1) Sindhu,Raveendran (1) Soccol,Carlos Ricardo (1) ÁVILA MARTÍNEZ,Elsa Giovanna (1) Mais... Palavra-chave Bacillus firmus (5) Cyclodextrin glycosyltransferase (3) Biodegradation (1) Biopolymer (1) Characterization (1) Cyclodextrins (1) Lactobacillus delbrueckii (1) Mixed culture (1) Poly-3-hydroxybutyrate (1) Pseudomonas fluorescens (1) Restauración (1) Starch (1) Mais... Tipo do documento Info:eu-repo/semantics/article (5) Ano 2015 (1) 2014 (1) 2004 (2) 2003 (1) País Brazil (4) Colombia (1) Idioma Inglês (4) Espanhol (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Colorimetric determination of alpha and beta-cyclodextrins and studies on optimization of CGTase production from B. firmus using factorial designs 52 Higuti,Ilma Hiroko; Silva,Priscila Anunciação da; Papp,Juliana; Okiyama,Vivian Mayumi de Eiróz; Andrade,Edicléia Alves de; Marcondes,Aluizio de Abreu; Nascimento,Aguinaldo José do. Cyclodextrin glycosyltransferase (EC 2.4.1.19, CGTase) production from B. firmus, isolated from soil of Curitiba, PR, was optimized in shake flask using an experimental design approach. The CGTase was produced when the carbon source was starch and beta-CD, but when simple sugars such as glucose, galactose, lactose, sucrose, and maltose were used, there was no enzyme production. CGTase production was the same with either organic nitrogen or inorganic nitrogen source. CGTase activity decreased 2-fold when incubation temperature was increased from 28 to 37 ° C, and decreased 2.1- fold when the initial pH was lowered from 10.3 to 7.4. The colorimetric determinations of alpha - and beta -CD were analyzed as a non-linear relationship and the equilibrium constant... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Cyclodextrins. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600001 Isolation of alkalophilic CGTase-producing bacteria and characterization of cyclodextrin-glycosyltransferase 52 Higuti,lma Hiroko; Grande,Simone Wichert; Sacco,Roberta; Nascimento,Aguinaldo José do. One hundred and twenty five soil samples were collected from the regions of roots of corn, cassava, potato, bean, sugar cane, soya, and pumpkin. From these, 75 strains were isolated that produced a yellowish halo surrounding the colonies, due to a phenolphtalein-cyclodextrin (CD) complex, and these were selected as alkalophilic CGTase-producing bacteria. All the 75 strains were identified as Bacillus firmus by microscopy and biochemical tests. The activity of the CGTase's varied from 2² to 2(10) dilutions,when assayed by CD-trichloroethylene (TCE)-complex precipitation. Strain 31 that produced the enzyme at the higher level was selected, and its enzyme was partially purified by starch adsorption (x 17) in a yield of 51%. Maximum enzyme activity occurred at... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Characterization. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200007 Mixed Cultures Fermentation for the Production of Poly-ß-hydroxybutyrate 52 Shalin,Thomas; Sindhu,Raveendran; Binod,Parameswaran; Soccol,Carlos Ricardo; Pandey,Ashok. Poly-ß-hydroxybutyrate (PHB) is a biodegradable intracellular microbial product produced by many bacteria and it is comparable to some of the petrochemical derived thermoplastics such as polypropylene. One of the main barriers for the commercial exploitation is the high cost of the substrate for the production of biopolymer. The utilization of mixed microbial cultures facilitates the use of complex substrates thereby reducing the cost of PHB production. In the present study, mixed culture systems were evaluated for PHB production. Bacillus firmus NII 0830 was used for the production of PHB since it accumulates a large amount of PHB and a second organism Lactobacillus delbrueckii NII 0925 was used to provide lactic acid. FTIR and 1H NMR analyses revealed... Tipo: Info:eu-repo/semantics/article Palavras-chave: Poly-3-hydroxybutyrate; Biopolymer; Mixed culture; Bacillus firmus; Lactobacillus delbrueckii; Biodegradation. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500644 PROMOCIÓN DEL CRECIMIENTO DE Baccharis macrantha (ASTERACEAE) CON BACTERIAS SOLUBILIZADORAS DE FOSFATOS ASOCIADAS A SU RIZOSFERA 37 ÁVILA MARTÍNEZ,Elsa Giovanna; LIZARAZO FORERO,Luz Marina; CORTÉS PÉREZ,Francisco. El objetivo de esta investigación fue aislar y caracterizar bacterias solubilizadoras de fosfatos (BSF) asociadas a la rizosfera de Baccharis macrantha y Viburnum triphyllum, y evaluar su capacidad para solubilizar fosfatos en condiciones in vitro. Además se determinó el efecto de la inoculación de las cepas de BSF más eficientes sobre el crecimiento de B. macrantha. Las muestras de suelo rizosférico de B. macrantha y V. triphyllum fueron colectadas en los meses de mayo-período de lluvia y septiembre-período seco del 2012. Para la cuantificación de bacterias heterótrofas cultivables y BSF se empleó el método de recuento en placa en los medios Agar Tripticasa de Soya y Pikovskaya (PVK) respectivamente. La capacidad de solubilización de fosfatos de las cepas... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus firmus; Pseudomonas fluorescens; Restauración. Ano: 2015 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000300010 Studies on alkalophilic CGTase-producing bacteria and effect of starch on cyclodextrin-glycosyltransferase activity 52 Higuti,Ilma Hiroko; Silva,Priscila Anunciação da; Nascimento,Aguinaldo José do. The production of beta- and gamma -CD by CGTase from Bacillus firmus was studied, in regard to the effect of the source and concentration of starch on the yield of CD and CGTase activity. A cyclic activity of accumulation and consumption of beta -CD and gamma -CD occurred during the bacterial growth. CGTase was more active when citrate buffer, pH 5.5 was used. No differences were found for production of gamma -CD with the use of commercial starch flour when compared with corn starch (p>0.05). The use of commercial starch flour resulted in decreased conversion of starch to beta-CD (all the 4 starch sources were statistically different, p<0.05). The best results for beta -CD production were obtained with the use of corn starch. The specific activity of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Starch. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100018 Registros recuperados: 5 Primeira ... 1 ... Última

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