SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Saxena,Rajshree; Singh,Rajni.

Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50ºC and 6 respectively. The enzyme was found to be thermostable at 70ºC for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na+, Ca++, Mg++ and Co++ enhanced the enzyme activity.

Application of bacterial and yeast biosurfactants for enhanced removal and biodegradation of motor oil from contaminated sand

Palavras-chave: Solid State Fermentation; Agro-industrial wastes; Amylase; Enzyme optimization; Stability; Bacillus sp.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400014

Electron. J. Biotechnol.

Chaprão,Marcos J; Ferreira,Isabela N.S; Correa,Priscilla F; Rufino,Raquel D; Luna,Juliana M; Silva,Elias J; Sarubbo,Leonie A.

Background This study investigated the potential application of two biosurfactants for enhanced removal capability and biodegradation of motor oil contaminated sand under laboratory conditions. The biosurfactants were produced by the yeast Candida sphaerica and by the bacterium Bacillus sp. cultivated in low-cost substrates. The ability of removing motor oil from soil by the two biosurfactants was identified and compared with that of the synthetic surfactants Tween 80 and Triton X-100. Results Both crude and isolated biosurfactants showed excellent effectiveness on motor oil removal from contaminated sand under kinetic conditions (70-90%), while the synthetic surfactants removed between 55 and 80% of the oil. A contact time of 5-10 min under agitation...

Tipo: Journal article

Palavras-chave: Bacillus sp; Bioremediation; Candida sphaerica; Petroleum; Sand-packed column.

Ano: 2015

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600014

Bacterial treatment of alkaline cement kiln dust using Bacillus halodurans strain KG1

Kunal,; Rajor,Anita; Siddique,Rafat.

Abstract This study was conducted to isolate an acid-producing, alkaliphilic bacterium to reduce the alkalinity of cement industry waste (cement kiln dust). Gram-positive isolate KG1 grew well at pH values of 6–12, temperatures of 28–50 °C, and NaCl concentrations of 0–16% and thus was further screened for its potential to reduce the pH of an alkaline medium. Phenotypic characteristics of the KG1 isolate were consistent with those of the genus Bacillus, and the highest level of 16S rRNA gene sequence similarity was found with Bacillus halodurans strain DSM 497 (94.7%). On the basis of its phenotypic characteristics and genotypic distinctiveness from other phylogenetic neighbors belonging to alkaliphilic Bacillus species, the isolated strain was designated...

Characterization of a novel lipase from Bacillus sp. isolated from tannery wastes

Palavras-chave: Alkaliphiles; Alkalinity reduction; Bacillus sp; Cement kiln dust.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100001

Ghori,M. I; Iqbal,M. J; Hameed,A.

Kinetics of a lipase isolated from Bacillus sp. was studied. The enzyme showed maximum activity at pH 9 and temperature 60ºC. The Michaelis constant (K M 0.31 µM) obtained from three different plots i.e., Lineweaver-Burk, Hanes-Wolf and Hofstee, was found to be lower than already reported lipases that confirmed higher affinity of the enzyme for its substrate p-NPL (p-nitrophenyl laurate). Vmax of the enzyme was found to be 7.6 µM/mL/min. Energy of activation calculated from Arrhenius plot was found to be 20.607 kJmol-1. Activation enthalpy (ΔH*) had negative trend and the value for the hydrolysis of p-NPL by the enzyme at optimum temperature was -2.748 kJmol-1 . Activation entropy (ΔS*) and free energy of activation (ΔG*) of the enzyme were found to be...

Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria

Palavras-chave: Bacillus sp; Kinetic study; Lipases; P-Nitrophenyl laurate; Tannery wastes; Organic solvents.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100003

ArchiMer

Ortega Morales, Benjamín Otto; Santiago Garcia, J.l; Chan Bacab, M.j.; Moppert, Xavier; Miranda Tello, E; Fardeau, M.l.; Carrero, J.c.; Bartolo Perez, P.; Valadez Gonzalez, A; Guezennec, Jean.

Aims: This study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (EPS). Methods and Results: A screening procedure was implemented to detect EPS-producing biofilm bacteria. Isolates MC3B-10 and MC6B-22, identified respectively as a Microbacterium species and Bacillus species by 16S rDNA and cellular fatty acids analyses, produced different EPS, as evidenced by colorimetric and gas chromatographic analyses. The polymer produced by isolate MC3B-10 displays significant surfactant activity, and may chelate calcium as evidenced by spectroscopic analysis. Conclusions: Polymer MC3B-10 appears to be a glycoprotein, while EPS MC6B-22 seems to be a true polysaccharide dominated by neutral sugars...

Tipo: Text

Palavras-chave: Tropical intertidal biofilm bacteria; Surfactants; Microbacterium sp; Exopolymers; Bacillus sp; Anionic.

Ano: 2007

URL: http://archimer.ifremer.fr/doc/2007/publication-2530.pdf

Controle da mancha foliar de Eucalyptus grandis e Eucalyptus urophylla causada por Cylindrocladium sp. com Bacillus sp.

Culture conditions for the production of thermostable amylase by Bacillus sp

BETTIOL, W.; AUER, C. G.; CAMARGO, L. E. A.; KIMATI, H..

Folhas destacadas, de plantas sadias de E. grandis e E. urophilla foram mantidas em placas de Petri de plastico contendo um disco de papel de filtro previamente umedecido com agua destilada, para estudar o controle de Cylindrocladium sp. usando um isolado de Bacillus sp., o qual apresentou grande eficiencia do crescimento micelial deste fungo in vitro. Uma cultura liquida (batata-dextrose) de Bacillus sp., com 10 dias de idade, foi uniformemente aplicada, com o auxilio de um pulverizador De Vibis, sobre folhas de E. grandis e E. urophylla 1, 24 e 48 horas antes da inoculacao de 0,1 ml de suspensao de esporos de Cylindrocladium sp., com auxilio de um pipetador. Esses tratamentos apresentaram controle semelhante ao Benomyl. Por outro lado, houve controle...

Tipo: Parte de livro

Palavras-chave: Bacillus sp; Antagonismo; Controle Biológico; Cylindrocladium sp; Eucalipto; Doença; Fungo; Mancha Foliar.

Ano: 1986

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/9854

Teodoro,Carlos Eduardo de Souza; Martins,Meire Lelis Leal.

Studies on the alpha-amylase production were carried out with a bacterial strain isolated from a soil sample. The cells were cultivated in a mineral medium containing soluble starch as sole carbon source. The addition of calcium (10 mM) or peptone (1%) and yeast extract (0.5%) to the mineral medium shortened the lag period and improved the growth and alpha-amylase synthesis. The addition of glucose to the culture diminished greatly the synthesis of alpha-amylase, demonstrating that a classical glucose effect is operative in this organism. The optimum temperature and initial medium pH for amylase synthesis by the organism were 50°C and 7.0 respectively. The optimal pH and temperature for activity were 6.0 and 50°C respectively. The enzyme extract retained...

Densidade populacional de Bacillus sp. em plantio de feijão precedido por diferentes plantas de cobertura.

Palavras-chave: Alpha-amylase; Thermophilic bacterium; Thermostable enzymes; Bacillus sp.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400011

BASÍLIO, J. P.; LOBO JUNIOR, M..

O objetivo deste trabalho foi avaliar o efeito do cultivo de diferentes plantas de cobertura na densidade populacional de Bacillus sp.

Tipo: Anais e Proceedings de eventos

Palavras-chave: Bacillus sp; Densidade populacional; Feijão; Phaseolus Vulgaris; Planta de Cobertura.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122762

Effect of the culture conditions on the production of an extracellular protease by thermophilic Bacillus sp and some properties of the enzymatic activity

Silva,Camila Rocha da; Delatorre,Andréia Boechat; Martins,Meire Lelis Leal.

Protease production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid cultures containing 1% maltose as a carbon source and supplemented with whey protein (0.1%) and corn steep liquor (0.3%) reached a maximum at 14 h, with levels of 42 U/mg protein. The microorganism was capable of utilizing a wide range of carbon sources, but protease activity varied according the carbon source. Starch and maltose were the best carbon sources in the present study for protease secretion, while lactose and sucrose were less effective. Increasing maltose concentration in the medium until 1%, improved the growth of the organism and the enzyme activity. Regarding the amounts of corn steep liquor and whey protein in the medium, the concentrations of 0.2% and 0.1%...

Effect of the medium composition on formation of amylase by Bacillus sp

Palavras-chave: Protease; Thermophilic bacterium; Bacillus sp.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200012

BABT

Santos,Eliana de Oliveira.; Martins,Meire Lelis Leal.

Studies on the alpha -amylase synthesis was carried out with a moderately thermophilic, facultatively anaerobic Bacillus sp, isolated from soil samples. The cells were cultivated in a complex medium containing soluble starch or maltose as carbon source. The levels of the alpha -amylaseactivity detected in culture supernatants varied greatly with the type of carbon source used. Maltose, soluble starch and citrate stimulated alpha -amylaseformation. Addition of exogenous glucose repressed formation of alpha -amylase, demonstrating that a classical glucose effect was operative in this organism. The concentration of yeast extract was found to be important factor in the alpha -amylase synthesis bythe isolate.The activity of the enzyme increased between 2 and 5...

Germinação e vigor de girassol com uso de bactérias promotoras de crescimento.

Palavras-chave: Alpha-amylase; Thermophilic bacterium; Bacillus sp.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100018

Inoculation and co-inoculation with multifunctional rhizobacteria for the initial development of soybean.

SILVA, M. E. C. F. da; MELO, I. S. de; NASCIMENTO, R. dos S.; ROSSI, P.; RAMOS, N. P..

Resumo: O objetivo deste estudo foi avaliar a germinação de sementes e o vigor de genótipos (BRS 321, 323, 417) de girassol (Helianthus annuus) tratados com quatro isolados de bactérias promotoras de crescimento. O estudo foi realizado no Laboratório de Microbiologia Ambiental (LMA) e Setor de Campos Experimentais (SCE) da Embrapa Meio Ambiente ? Jaguariúna (SP), com avaliação realizada em sementes e plantas desenvolvidas. Os seguintes valores foram determinados: vigor; anormais; mortas; primeira contagem (PCG); germinação (G); comprimento (CP) e biomassa total de plântulas (BTP) em laboratório; e: velocidade de emergência (VE); emergência (E); mortas (M); biomassa da parte aérea de plântulas (BPA); altura de plantas (AP); número de folhas (NF); e biomassa...

Tipo: Anais e Proceedings de eventos

Palavras-chave: Desenvolvimento; Paenibacillus sp; Bacillus sp; Helianthus annuus; Girassol; Pseudomonas putida; Estimulante de Crescimento Vegetal; Bactéria; Emergência; Growth promotion; Growth regulators.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116869

Produção de poligalacturonase, pelo termofílico Bacillus sp. e algumas de suas propriedades

SILVA, M. A.; CRUZ, D. R. C.; FRASCA, L. L. de M.; FILIPPI, M. C. C. de; FERREIRA, A. L.; NASCENTE, A. S..

Soybean inoculation and co-inoculation with multifunctional rhizobacteria is a sustainable alternative that may contribute to plant growth and increased agricultural production, making the product more competitive, as well as reducing costs for the producer. This study aimed to evaluate the effect of inoculation and co-inoculation with multifunctional Serratia sp. and Bacillus sp. rhizobacteria on the early development of soybean. A completely randomized experimental design was used, with four treatments and ten replicates, totaling 40 experimental plots. The treatments consisted of the microbiolization of soybean seeds and a control treatment: BRM 32114 (Serratia sp.) isolate; BRM 63573 (Bacillus sp.) isolate (formerly named 1301); co-inoculation with BRM...

Tipo: Artigo de periódico

Palavras-chave: Bacillus sp; Rhizobacteria; Soja; Glycine Max; Inoculação; Serratia; Soybeans; Inoculation methods.

Ano: 2022

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149542

Ciênc. Tecnol. Aliment.

Cordeiro,Carlos Alberto Martins; Martins,Meire Lelis Leal.

A produção de poligalacturonase pelo termofílico Bacillus sp. SMIA-2, cultivado em meio líquido contendo pectina cítrica como única fonte de carbono, alcançou a sua máxima atividade enzimática em 30 horas, com níveis de 42 IU.mL-1. Entre as várias fontes orgânicas e inorgânicas de nitrogênio testadas, o sulfato de amônio foi a que proporcionou maior atividade da poligalacturonase. O aumento da concentração da pectina cítrica, no meio de cultura, acima de 0,5% não proporcionou um aumento da atividade da enzima. O microrganismo foi capaz de utilizar uma variedade de fontes de carbono, mas a atividade da poligalacturonase variou com cada fonte. Pectina de maçã foi a melhor fonte de carbono para a secreção da poligalacturonase (56 IU.mL-1), enquanto frutose e...

Produção de proteases por Bacillus sp SMIA-2 crescido em soro de leite e água de maceração de milho e compatibilidade das enzimas com detergentes comerciais

Palavras-chave: Poligalacturonase; Bactéria termofílica; Bacillus sp.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612009000100021

Ciênc. Tecnol. Aliment.

Nascimento,Wellingta Cristina Almeida do; Martins,Meire Lelis Leal.

A produção de proteases por Bacillus sp. SMIA-2 cultivado em um meio de cultura contendo soro de leite e água de maceração de milho foi estudada. Além disso, a compatibilidade da enzima com detergentes comerciais foi também avaliada. A atividade máxima da enzima (70 U/mg proteína) foi observada na fase estacionária de crescimento, com 32 h de incubação. Estudos sobre a caracterização da protease revelaram que a temperatura ótima para atividade desta enzima foi 70 °C e que a mesma manteve 91% de sua atividade quando incubada a 70 °C na presença do cálcio. O valor ótimo de pH encontrado para a protease foi 8,0, sendo que a enzima manteve 85% e 46% de sua atividade quando incubada por 1 h em pH 9 e pH 10 respectivamente. A protease manteve 64% e 50% de sua...

Production and properties of Xylanase from thermophilic Bacillus sp.

Palavras-chave: Proteases; Soro de leite; Detergentes comerciais; Bacillus sp.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612006000300016

BABT

Cordeiro,Carlos Alberto Martins; Martins,Meire Lelis Leal; Luciano,Angélica Bárbara; Silva,Roberta Freitas da.

An aerobic, thermophilic, xylanolytic bacterium was isolated from local soil. The results of 16S rRNA sequence comparisons indicated that the isolate was closely related to Bacillus caldoxylolyticus and Bacillus sp strain AK1. These organisms exhibited 94% levels of ribossomal DNA sequence homology. Studies on the xylanase characterisation from liquid cultures grown on beechwood xylan revealed that the enzyme retained 100% of activity for 2 hours at temperatures ranging from 30 to 50º C, while at 60, 70 and 100º C, 10%, 11% and 29% of the original activities were lost, respectively. The optimum pH of the enzyme was found to be between 6.5 and 7.0. After incubation of crude enzyme solution for 24 hours at 25º C and at pH 5.5 to 8.0, a decrease of about 12%...

Production of extracellular alkaline protease by new halotolerant alkaliphilic Bacillus sp. NPST-AK15 isolated from hyper saline soda lakes

Palavras-chave: Xylanase; Thermophilic bacterium; Bacillus sp.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600002

Electron. J. Biotechnol.

Ibrahim,Abdelnasser S.S; Al-Salamah,Ali A; Elbadawi,Yahya B; El-Tayeb,Mohamed A; Ibrahim,Shebl Salah Shebl.

Background Alkaline proteases are among the most important classes of industrial hydrolytic enzymes. The industrial demand for alkaline proteases with favorable properties continues to enhance the search for new enzymes. The present study focused on isolation of new alkaline producing alkaliphilic bacteria from hyper saline soda lakes and optimization of the enzyme production. Results A new potent alkaline protease producing halotolerant alkaliphilic isolate NPST-AK15 was isolated from hyper saline soda lakes, which affiliated to Bacillus sp. based on 16S rRNA gene analysis. Organic nitrogen supported enzyme production showing maximum yield using yeast extract, and as a carbon source, fructose gave maximum protease production. NPST-AK15 can grow over a...

Tipo: Journal article

Palavras-chave: Alkaline protease; Alkalophiles; Bacillus sp; Fermentation; Optimization.

Ano: 2015

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300015

Properties of an amylase from thermophilic Bacillus SP.

Carvalho,Raquel Vieira de; Côrrea,Thamy Lívia Ribeiro; Silva,Júlia Caroline Matos da; Mansur,Luciana Ribeiro Coutinho de Oliveira; Martins,Meire Lelis Leal.

alpha-Amylase production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid cultures containing soluble starch as a carbon source and supplemented with 0.05% whey protein and 0.2% peptone reached a maximum activity at 32 h, with levels of 37 U/mL. Studies on the amylase characterization revealed that the optimum temperature of this enzyme was 90ºC. The enzyme was stable for 1 h at temperatures ranging from 40-50ºC while at 90ºC, 66% of its maximum activity was lost. However, in the presence of 5 mM CaCl2, the enzyme was stable at 90ºC for 30 min and retained about 58% residual activity after 1 h. The optimum pH of the enzyme was found to be 8.5. After incubation of enzyme for 2 h at pH 9.5 and 11.0 was observed a decrease of about 6.3% and...

Protease with collagenolytic activity produced by Bacillus sp. DPUA 1728 from Amazonian soil

Palavras-chave: Alpha-Amylase; Thermophilic bacterium; Bacillus sp.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100023

Lima,Lorena A.; Cruz Filho,Raimundo F.; Santos,Januário G. dos; Silva,Wilson C..

Qualitative analyses were carried out on solid medium with insoluble collagen 0.25% (w/v) to detect proteases with collagenolytic activity produced by Bacillus sp. In cultures incubated for 24 h, a 23 full factorial design with four repetitions at the center point was developed to analyze the effects and interactions between initial pH, temperature and the concentration of gelatin. Based on the results of the first 23 full factorial design, a successive 23 full factorial design was performed. The most favorable production conditions were found to be 1.5% (w/v) gelatin, pH 9.0 and 37 °C with enzymatic activity of 86.27 U/mL. The enzyme showed optimal activity at 50 °C and pH 9.0, and it was stable over wide pH (7.2-10.0) and temperature (45 °C-60 °C)...

Screening of bacterial strains for pectinolytic activity: characterization of the polygalacturonase produced by Bacillus sp

Palavras-chave: Collagenolytic protease; Bacillus sp; Factorial design; Amazonian soil.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401217

Rev. Microbiol.

Soares,Márcia M.C.N.; Silva,Roberto da; Gomes,Eleni.

One hundred sixty eight bacterial strains, isolated from soil and samples of vegetable in decomposition, were screened for the use of citrus pectin as the sole carbon source. 102 were positive for pectinase depolymerization in assay plates as evidenced by clear hydrolization halos. Among them, 30% presented considerable pectinolytic activity. The cultivation of these strains by submerged and semi-solid fermentation for polygalacturonase production indicated that five strains of Bacillus sp produced high quantities of the enzyme. The physico-chemical characteristics, such as optimum pH of 6.0 - 7.0, optimum temperatures between 45oC and 55oC, stability at temperatures above 40oC and in neutral and alkaline pH, were determined.