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Antagonism of Bacillus spp. against Xanthomonas campestris pv. campestris BABT
Monteiro,Leila; Mariano,Rosa de Lima Ramos; Souto-Maior,Ana Maria.
The antagonism of eight Bacillus isolates was investigated against nine strains of Xanthomonas campestris pv. campestris (causal agent of crucifers black rot) to assess the role of lipopeptides in this process. Antimicrobial and hemolytic (surfactant) activity tests were performed in vitro using agar diffusion methods. Antibiosis and hemolysis were positive for four Bacillus isolates against all X. campestris pv. campestris strains. The correlation observed between antimicrobial and hemolytic activities indicated that lipopeptides were involved in the antibiosis mechanism of the studied antagonists. Fermentation studies were carried out with the isolates that showed highest antimicrobial and hemolytic activities, to follow up growth and production of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Biological control; Xanthomonas campestris pv. campestris; Crucifers black rot.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100004
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Antagonismo de Bacillus spp. frente a hongos fitopatógenos del cultivo del arroz (Oryza sativa L.) Rev. Protección Veg.
Tejera,B; Heydrich,Mayra; Rojas,Marcia M.
El arroz es uno de los alimentos con mayor demanda en el mercado actual debido al gran número de personas que lo consumen. Una alternativa para dar solución a la aparición de enfermedades de origen fúngico es la utilización de bacterias como control biológico de hongos fitopatógenos. Dentro de estos microorganismos, los aislamientos del género Bacillus han sido uno de los más empleados en el control biológico, debido a que estos tienen la capacidad de producir una amplia diversidad de metabolitos con actividad antifúngica. En el presente trabajo se utilizaron siete aislados pertenecientes al género Bacillus para evaluar el efecto antagónico ejercido por estos microorganismos sobre dos hongos fitopatógenos del cultivo del arroz Curvularia sp. (Boed.) y...
Tipo: Journal article Palavras-chave: Bacillus; Curvularia sp.; Pyricularia grisea.
Ano: 2012 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522012000200008
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Autochthonous probiotic mixture improves biometrical parameters of larvae of Piaractus mesopotamicus (Caracidae, Characiforme, Teleostei) Ciência Rural
Guidoli,Marcos Gabriel; Mendoza,Jorge Arnaldo; Falcón,Sofia Lizardo; Boehringer,Silvia Irene; Sánchez,Sebastián; Macías,María Elena Fátima Nader.
ABSTRACT: Probiotics are a novel alternative to antibiotics as growth factors. Previously, our group isolated, selected and tested in vivo, eight autochthonous strains. They showed no significant effects when administered individually. However, the best doses, stages and ways of administration were combined in a multi strain formula. The aim of this research was to evaluate the effect of this probiotic product on the growth and survival of Piaractus mesopotamicus larvae. The administration was implemented during egg incubation and endogenous feeding period (5), during larvae exogenous feeding period (10) and all along the experiment (15). A group without microorganisms was used as control. The probiotic generates significant increments of mean weight and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactic acid bacteria; Bacillus; Pacú; Larviculture.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700652
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Avaliação de probióticos na dieta de frangos de corte criados em cama nova ou reutilizada R. Bras. Zootec.
Traldi,Ana Beatriz; Oliveira,Maria Cristina de; Duarte,Karina Ferreira; Moraes,Vera Maria Barbosa de.
Três experimentos foram conduzidos com o objetivo de avaliar o efeito da utilização de probióticos na dieta de frangos de corte sobre as características da cama reutilizada e das lesões de peito, joelho e coxim plantar. Foram utilizados em cada experimento 800 pintos machos de um dia, da linhagem comercial Ross, alojados em 20 boxes, em densidade populacional de 10 aves/m². O delineamento foi inteiramente casualizado e os tratamentos distribuídos em esquema fatorial 2 × 2 (cama nova ou reutilizada x rações com ou sem probiótico). O probiótico utilizado nas dietas foi composto por Bacillus subtilis e B. coagulans (2x10(9) e 1x10(7) ufc, respectivamente). Aos 42 dias de idade, quatro aves de cada boxe foram abatidas para análise das lesões de peito, joelho e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Qualidade da cama de frango; PH; Potencial de volatilização de amônia.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982007000300020
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Bioaccumulation of copper, zinc, cadmium and lead by Bacillus sp., Bacillus cereus, Bacillus sphaericus and Bacillus subtilis BJM
Costa,Antonio Carlos Augusto da; Duta,Flavia Pereira.
This work presents some results on the use of microbes from the genus Bacillus for uptake of cadmium, zinc, copper and lead ions. Maximum copper bioaccumulations were 5.6 mol/g biomass for B. sphaericus, 5.9 mol/g biomass for B. cereus and B. subtilis, and 6.4 mol/g biomass for Bacillus sp. Maximum zinc bioaccumulations were 4.3 mol/g biomass for B. sphaericus, 4.6 mol/g biomass for B. cereus, 4.8 mol/g biomass for Bacillus sp. and 5.0 mol/g biomass for B. subtilis. Maximum cadmium bioaccumulations were 8.0 mol/g biomass for B. cereus, 9.5 mol/g biomass for B. subtilis, 10.8 mol/g biomass for Bacillus sp. and 11.8 mol/g biomass for B. sphaericus. Maximum lead biomaccumulations were 0.7 mol/g biomass for B. sphaericus, 1.1 mol/g biomass for B. cereus, 1.4...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioaccumulation; Bacillus; Heavy metals.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100001
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Bioprospecting endophytic bacteria for biological control of coffee leaf rust Scientia Agricola
Shiomi,Humberto Franco; Silva,Harllen Sandro Alves; Melo,Itamar Soares de; Nunes,Flávia Vieira; Bettiol,Wagner.
Suppression of plant diseases due to the action of endophytic microorganisms has been demonstrated in several pathosystems. Experiments under controlled conditions involving endophytic bacteria isolated from leaves and branches of Coffea arabica L and Coffea robusta L were conducted with the objective of evaluating the inhibition of germination of Hemileia vastatrix Berk. & Br., race II, urediniospores and the control of coffee leaf rust development in tests with leaf discs, detached leaves, and on potted seedling of cv. Mundo Novo. The endophytic bacterial isolates tested proved to be effective in inhibiting urediniospore germination and/or rust development, with values above 50%, although the results obtained in urediniospore germination tests...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemileia vastatrix; Coffea spp.; Bacillus; Biocontrol.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000100006
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Cepa antártica de Bacillus sp., con actividad extracelular de tipo agarolítica y alginatoliasa Gayana
Lavin,Paris; Gallardo-Cerda,Jorge; Torres-Diaz,Cristian; Asencio,Geraldine; Gonzalez,Marcelo.
Diversas bacterias asociadas a macroalgas pueden usar ficocoloides como fuente de carbono. Las bacterias de la Antártica, poseen características fisiológicas adaptativas que habrían evolucionado para permitir su supervivencia y funcionamiento dentro de las condiciones adversas de ese ecosistema. Por lo tanto, bacterias aisladas desde algas antárticas podrían tener la capacidad de degradar enzimáticamente azúcares complejos a temperaturas inferiores que aquellas aisladas desde zonas más cálidas, lo que potencialmente podría tener aplicaciones en la mejora en procesos industriales que utilizan enzimas. El análisis del gen ribosomal 16S indica que la cepa bacteriana aislada desde algas varadas en la Isla Rey Jorge (Antártica), pertenece al género Bacillus. El...
Tipo: Journal article Palavras-chave: Bacillus; Antártica; Agarasa; Alginatoliasa.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382013000200001
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Characterization of Bacillus isolates of potato rhizosphere from andean soils of Peru and their potential PGPR characteristics BJM
Calvo,Pamela; Ormeño-Orrillo,Ernesto; Martínez-Romero,Esperanza; Zúñiga,Doris.
Bacillus spp. are well known rhizosphere residents of many crops and usually show plant growth promoting (PGP) activities that include biocontrol capacity against some phytopatogenic fungi. Potato crops in the Andean Highlands of Peru face many nutritional and phytophatogenic problems that have a significant impact on production. In this context is important to investigate the natural presence of these microorganisms in the potato rhizosphere and propose a selective screening to find promising PGP strains. In this study, sixty three Bacillus strains isolated from the rhizosphere of native potato varieties growing in the Andean highlands of Peru were screened for in vitro antagonism against Rhizoctonia solani and Fusarium solani. A high prevalence (68%) of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Plant growth-promoting rhizobacteria; Potato; Antagonism; Phosphate solubilization; 16S rRNA gene.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400008
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Characterization of pyrene utilizing Bacillus spp. from crude oil contaminated soil BJM
Khanna,Purnima; Goyal,Dinesh; Khanna,Sunil.
Pyrene, a high molecular weight polycyclic aromatic hydrocarbon (PAH), is a priority pollutant present in soil contaminated with crude oil, coal-tar and complex PAHs. Bacterial consortium CON-3 developed from crude oil contaminated soil of Patiala, Punjab (India) cometabolized 50 µg ml-1 pyrene in the presence of glucose (0.5 %; w/v) at 30 °C, as determined by reverse-phase high performance liquid chromatography (HPLC). Bacillus sp. PK-12, Bacillus sp. PK-13 and Bacillus sp. PK-14 from CON-3, identified by 16S rRNA gene sequence analysis, were able to cometabolize 64 %, 55 % and 53 % of pyrene in 35 days, respectively. With the increase in glucose concentration to 1.0 % (w/v) in growth medium isolates PK-12, PK-13 and PK-14 showed 19 - 46 % uptake of 50 µg...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pyrene; Bacillus; Crude oil contaminated soil; High performance liquid chromatography (HPLC).
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200024
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Contribution du genre bacillus à la dégradation bactérienne des hydrocarbures ArchiMer
Willemse-collinet, M.f.; Carsin, J.l.; Brodard, E.; L'Emeillat, Y..
Evolution of marine oil pollution made the study of microbial degradation a current matter of importance. The present work dealt with preliminary assays in vitro of a sys­tematic study of petroleum degrading Bacillus species. Degradation of petrol during the different experimentations was followed with infra­red analyses. Progressive elimination of alcanes and isoalcanes was controlled by gas- chromatography. Out of the 37 strains of Bacillus subtilis, B_. polymyxa, B. megaterium and Sporosar- cina tested, 10 could grow with petrol as unique source of carbon. The factors of growth as supplemented in these experiments hardly accelerated the degradation.
Tipo: Text Palavras-chave: Pétrole; Biodégradation; Bacillus.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/00306/41750/40961.pdf
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Control biológico de la marchitez del chile (Capsicum annuum L.) por Bacillus thuringiensis Phyton
Mojica-Marín,V; Luna-Olvera,HA; Sandoval-Coronado,CF; Pereyra-Alférez,B; Morales-Ramos,LH; González-Aguilar,NA; Hernández-Luna,CE; Alvarado-Gomez,OG.
La expansión de la marchitez del chile en nuevas plantaciones y diferentes zonas productoras de México, y la dificultad en el control de la misma, obligan a desarrollar nuevas medidas de control. Se espera que las mismas auspicien mejores expectativas para el control de la enfermedad en el contexto de una estrategia de manejo integrado. El presente estudio se realizó con el objetivo de evaluar y determinar la capacidad antagónica de 64 cepas de Bacillus thuringiensis sobre Rhizoctonia solani, Phytophthora capsici y Fusarium oxysporum mediante estudios de cultivos duales en placas Petri. Las cepas de Bacillus thuringiensis provinieron de la Colección Internacional de Bacilos Entomopatógenos de la FCB-UANL. Fueron 16 cepas de B. thuringiensis que redujeron...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Control biológico; Antagonismo; Germinación; Fitopatógenos con origen en el suelo.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572009000200004
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Co-production of Proteases and Bioactive Protein Hydrolysates from Bioprocessing of Feather Meal BABT
Bernardo,Bernardete da Silva; Ramos,Rodrigo Ferraz; Callegaro,Kelly; Daroit,Daniel Joner.
Abstract: Feather meal conversion through submerged cultivations with Bacillus strains (CL33A, CL14) yielded proteases and protein hydrolysates. After 4-day (CL33A) and 10-day (CL14) cultivations, protease activities reached 461 U/mL; hydrolysates presented antioxidant (radicals-scavenging, 57-77%; Fe2+-chelation, 14-28%; Fe3+-reduction) and antidiabetic (dipeptidyl peptidase-IV inhibition, 49-52%) potentials. The obtained bioproducts present prospective commercial/industrial applications.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Bioprocess; Enzyme; Antioxidant; Antidiabetic.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100220
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Diversidad y caracterización de poblaciones bacterianas asociadas a esclerocios de Claviceps gigantea Fuentes et al. en maíz (Zea mays L.) con potencial antagonista, en el Estado de México. Colegio de Postgraduados
Ayala Torres, Ana María.
Un total de 129 aislamientos bacterianos obtenidos de la micósfera de esclerocios de Claviceps gigantea se evaluaron in vitro para determinar su capacidad de inhibir el crecimiento de Fusarium graminearum, F. subglutinans y F. verticillioides patógenos a maíz. Ensayos cualitativos en confrontación dual, resultaron en la selección de 22 aislamientos bacterianos con el mayor potencial antagónico hacia una, dos o las tres especies de Fusarium. Los aislamientos se identificaron molecularmente mediante secuenciación parcial del gen 16s ADNr. Las cepas con mayor grado de antagonismo pertenecen a los géneros Bacillus y Pseudomonas. La caracterización en la producción de metabolitos potencialmente involucrados en la actividad antagónica, mostró que el 100% de las...
Palavras-chave: Bacillus; Pseudomonas; Biocontrol; Metabolitos secundarios; Caracterización; Secondary metabolites; Characterization; Fitopatología; Maestría.
Ano: 2014 URL: http://hdl.handle.net/10521/2299
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Efficacy of chemical and biological strategies for controlling the soybean brown spot (Septoria glycines) Ciencia e Investigación Agraria
Mantecón,Jorge D.
Soybean {Glycine max) is currently the most widely grown and rapidly expanding crop in Argentina, and brown spot caused by Septoria glycines is a major foliage disease that affects this crop. Triáis in the 2005 and 2006 growing seasons were undertaken using soybean (Glycine max) cv. Nidera A4800 RR. A randomized complete block design was used with four replicates. Two biological fungicides, based on Bacillus subtilis or B. pumilus, and four conventional fungicides, pyraclostrobin in combination with epoxiconazole (Opera 25 SC, BASF Argentina), azoxystrobin (Amistar 25 SC, Syngenta Argentina), trifloxystrobin in combination with cyproconazole (Sphere 267,5 EC, Bayer, Argentina), and tebuconazole (Folicur 25 EC, Bayer, Argentina), were evaluated. Fungicides...
Tipo: Journal article Palavras-chave: Bacillus; Chemical and biological control; Brown spot; Diseases; Fungicides; Soybean.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202008000200011
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Enhanced production of dimethyl phthalate-degrading strain Bacillus sp. QD14 by optimizing fermentation medium Electron. J. Biotechnol.
Mo,Jixian; Wang,Zhigang; Xu,Weihui; Li,Shanshan; Yu,Zhidan.
Background Integrated statistical experimental designs were applied to optimize the medium constituents for the production of a dimethyl phthalate (DMP)-degrading strain Bacillus sp. QD14 in shake-flask cultures. A Plackett-Burman design (PBD) was applied to screen for significant factors, followed by the Steepest Ascent Method (SAM) to find the nearest region of maximum response. A Box-Behnken design (BBD) of the Response Surface Methodology (RSM) was conducted to optimize the final levels of the medium components. Results After the regression equation and response surface contour plots were analyzed, the concentrations of glucose, corn meal and NaCl were found to significantly influence the biomass of DMP-degrading bacteria. A combination of 22.88 g/L of...
Tipo: Journal article Palavras-chave: Bacillus; Box-Behnken design; Dimethyl phthalate; Medium optimization; Plackett-Burman design.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300016
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Evaluación in vitro de la formación de biopelículas de bacterias marinas del Caribe colombiano Boletín de Investigaciones
López,Sofía; Zea,Sven; Gómez-León,Javier.
RESUMEN La formación de biopelículas es un proceso importante para las comunidades bacterianas marinas debido a que este mecanismo favorece la adaptación a variaciones en las condiciones ambientales. El objetivo de este estudio fue evaluar la formación de biopelículas de las bacterias aisladas de sedimentos marinos en condiciones in vitro. Para esto se realizaron ensayos de cuantificación de biopelículas usando dos métodos, cristal violeta y reducción del colorante XTT; además se evaluó la viabilidad de las bacterias mediante la tinción Live/Dead. Posteriormente las cepas evaluadas fueron identificadas usando el marcador 16S ARN. Los resultados mostraron que los aislados pertenecen al género Bacillus todos en diferentes rangos fueron capaces de formar...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterias marinas; Bacillus; Biopelículas; Sedimentos.
Ano: 2019 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612019000200071
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Evaluation of Bacillus spp. as dough starters for Adhirasam - A traditional rice based fermented food of Southern India BJM
Anisha,Anvar Hussain Noorul; Anandham,Rangasamy; Kwon,Soon Woo; Gandhi,Pandiyan Indira; Gopal,Nellaiappan Olaganathan.
Abstract Adhirasam is a cereal based, doughnut shaped, deep fried dessert consumed in the southern regions of India. The dough used to prepare adhirasam is fermented and contains rice flour and jaggery. The aim of the present study was to characterize the cultivable bacteria associated with this fermented dough and to identify a suitable starter culture for the production of quality adhirasam. In total, one hundred and seventy bacterial isolates were recovered from de Man Rogosa Sharp (MRS) agar, nutrient agar, lysogeny agar and tryptic soy agar media. Out of the 170 bacterial isolates, sixteen isolates were selected based on their ability to tolerate glucose and sucrose. All the bacterial isolates tolerated 15% glucose and 30% sucrose. Analyses of 16S...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhirasam; Fermented cereal product; Bacillus; South Indian dessert.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401183
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Feather degradation by strains of Bacillus isolated from decomposing feathers BJM
Swetlana,Nagal; Jain,P. C..
Feather waste is generated in large amounts as a by-product of commercial poultry processing. This residue is almost pure keratin, which is not easily degradable by common proteolytic enzymes. Eight strains of Bacillus, isolated from decomposing feathers were tested for the hydrolysis of feather wastes in the laboratory. Among these strains, Bacillus cereus KB043 was the best feather degrading organism when grown on basal medium containing 1% hen feather as sole source of carbon and nitrogen. It caused 78.16 ± 0.4 % degradation with a significant release of soluble protein (1206.15 ± 14.7 µg mL-1) and cysteine (20.63 ± 0.4 µg mL-1) in the cultivation fluid. The strain also showed the highest level of keratinase activity (39.10 ± 0.4 U mL-1). These data...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Feather degradation; Poultry waste; Soluble protein; Keratinase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100028
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In vitro degradation of linamarin by microorganisms isolated from cassava wastewater treatment lagoons BJM
Vasconcellos,S. P; Cereda,M. P.; Cagnon,J. R.; Foglio,M.A.; Rodrigues,R.A.; Manfio,G. P.; Oliveira,V. M..
This study aimed at isolating and characterizing of microorganisms able to use linamarin as sole carbon source. Thirty one microbial strains were isolated from manipueira, a liquid effluent of cassava processing factories. Among these strains, Bacillus licheniformis (isolate 2_2) and Rhodotorulla glutinis (isolate L1) were able to degrade 71% and 95% of added linamarin, respectively, within 7 days, showing high biodegradation activity and great potential for detoxification of cassava processing wastewaters.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Degradation; Manipueira; Fertirrigation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400019
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Inducible cellulase production from an organic solvent tolerant Bacillus sp. SV1 and evolutionary divergence of endoglucanase in different species of the genus Bacillus BJM
Thomas,Lebin; Ram,Hari; Singh,Ved Pal.
Abstract Bacteria are important sources of cellulases with various industrial and biotechnological applications. In view of this, a non-hemolytic bacterial strain, tolerant to various environmental pollutants (heavy metals and organic solvents), showing high cellulolytic index (7.89) was isolated from cattle shed soil and identified as Bacillus sp. SV1 (99.27% pairwise similarity with Bacillus korlensis). Extracellular cellulases showed the presence of endoglucanase, total cellulase and β-glucosidase activities. Cellulase production was induced in presence of cellulose (3.3 times CMCase, 2.9 times FPase and 2.1 times β-glucosidase), and enhanced (115.1% CMCase) by low-cost corn steep solids. An in silico investigation of endoglucanase (EC 3.2.1.4) protein...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Cellulases; Conserved signature indels.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200429
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