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Registros recuperados: 329
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A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide J. Venom. Anim. Toxins incl. Trop. Dis.
Clement,Herlinda; Flores,Vianey; Diego-Garcia,Elia; Corrales-Garcia,Ligia; Villegas,Elba; Corzo,Gerardo.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecticidal peptides; Protein expression; Bacteria; Theraphosid spider; Chemical synthesis; Cysteine-rich peptides.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100324
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A conservative region of the mercuric reductase gene (merA) as a molecular marker of bacterial mercury resistance BJM
Sotero-Martins,Adriana; Jesus,Michele Silva de; Lacerda,Michele; Moreira,Josino Costa; Filgueiras,Ana Luzia Lauria; Barrocas,Paulo Rubens Guimarães.
The most common bacterial mercury resistance mechanism is based on the reduction of Hg(II) to Hg0, which is dependent of the mercuric reductase enzyme (MerA) activity. The use of a 431 bp fragment of a conservative region of the mercuric reductase (merA) gene was applied as a molecular marker of this mechanism, allowing the identification of mercury resistant bacterial strains.
Tipo: Info:eu-repo/semantics/article Palavras-chave: MerA gene; Mercury resistant; Bacteria; Molecular marker.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200020
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A dual symbiosis shared by two mussel species, Bathymodiolus azoricus and Bathymodiolus puteoserpentis (Bivalvia : Mytilidae), from hydrothermal vents along the northern Mid-Atlantic Ridge ArchiMer
Duperron, Sebastien; Bergin, C; Zielinski, F; Blazejak, A; Pernthaler, A; Mckiness, Z; Dechaine, E; Cavanaugh, C; Dubilier, Nicole.
Bathymodiolus azoricus and Bathymodiolus puteoserpentis are symbiont-bearing mussels that dominate hydrothermal vent sites along the northern Mid-Atlantic Ridge (MAR). Both species live in symbiosis with two physiologically and phylogenetically distinct Gammaproteobacteria: a sulfur-oxidizing chemoautotroph and a methane-oxidizer. A detailed analysis of mussels collected from four MAR vent sites (Menez Gwen, Lucky Strike, Rainbow, and Logatchev) using comparative 16S rRNA sequence analysis and fluorescence in situ hybridization (FISH) showed that the two mussel species share highly similar to identical symbiont phylotypes. FISH observations of symbiont distribution and relative abundances showed no obvious differences between the two host species. In...
Tipo: Text Palavras-chave: Bacteria; Phylogeny; Fluorescence in situ hybridization (FISH); Methane oxidizer; Sulfur oxidizer; 16S rRNA; Endosymbiosis.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1864.pdf
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A laboratory model system for analysing microbial interactions occurring in anoxic estuarine sediments. ArchiMer
Herbert, R; Keith, S.
Defined mixed populations of Clostridium butyricum, Desulfovibrio desulfuricans and Chromatium vinosum co-existed for long periods when grown in a single stage chemostat with glucose as sole carbon source. The nature and availability of the inorganic nitrogen source exerted a major effect on carbon flow in the experimental system. The data show that both the C. butyricum and D. desulfuricans isolates can utilise NO sub(3) as an e super(-)acceptor with an increase in cell yield. Under these growth conditions the free S super(2-) levels were lower resulting in more stable mixed populations. In addition the data show that the C. butyricum produces more oxidised fermentation end-products (acetate) when NO sub(3) super(-) was available and more reduced products...
Tipo: Text Palavras-chave: Chromatium vinosum; Desulfovibrio desulfuricans; Clostridium butyricum; Biogeochemistry; Models; Bacteria; Detritus; Mineralization; Anoxic sediments; Estuarine sedimentation; Analytical techniques; Carbon cycle; Interspecific relationships; Microbiology.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-947.pdf
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A microbial community analysis of the octocoral Eunicea fusca Electron. J. Biotechnol.
Duque-Alarcón,Angela; Santiago-Vázquez,Lory Z; Kerr,Russell G.
While there is a significant and growing body of knowledge describing the microbial communities of marine invertebrates such as sponges, there are very few such studies focused on octocorals. The octocoral Eunicea fusca is common on reefs in various regions of the Caribbean and has been the subject of natural product investigations. As part of an effort to describe the microbial community associated with octocorals, a culture-independent analysis of the bacterial community of E. fusca was conducted. Specifically, a 16S rDNA clone library analysis was performed to provide baseline data. A total of 40 bacteria members from 11 groups were found. In general, Proteobacteria were the dominant group with a total of 24 species and α-Proteobacteria...
Tipo: Journal article Palavras-chave: 16S rDNA; Bacteria; Culture-independent analysis; Eunicea fusca; Microbial community; Octocoral.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500015
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A structural perspective on the mechanisms of quorum sensing activation in bacteria Anais da ABC (AABC)
LIXA,CAROLINA; MUJO,AMANDA; ANOBOM,CRISTIANE D.; PINHEIRO,ANDERSON S..
Bacteria are able to synchronize the population behavior in order to regulate gene expression through a cell-to-cell communication mechanism called quorum sensing. This phenomenon involves the production, detection and the response to extracellular signaling molecules named autoinducers, which directly or indirectly regulate gene expression in a cell density-dependent manner. Quorum sensing may control a wide range of biological processes in bacteria, such as bioluminescence, virulence factor production, biofilm formation and antibiotic resistance. The autoinducers are recognized by specific receptors that can either be membrane-bound histidine kinase receptors, which work by activating cognate cytoplasmic response regulators, or cytoplasmic receptors...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Autoinducer; Bacteria; Quorum sensing; Receptor; Structure.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502189
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A test of community assembly rules using foliar endophytes from a tropical forest canopy ArchiMer
Donald, Julian; Roy, Mélanie; Suescun, Uxue; Iribar, Amaia; Manzi, Sophie; Péllissier, Léonie; Gaucher, Philippe; Chave, Jérôme.
1.Community assembly theory assumes that ecological communities are spatially delimited into patches. Within these patches, coexistence results from environmental filtering, competition, and immigration. Truly delineated communities exist in laboratory studies of microbial cultures in Petri dishes, yet empirical tests conducted in continuous environments often use patches defined by convention as opposed to realised boundaries. 2.Here we perform a test of ecological community assembly rules using foliar endophyte communities from a tropical rainforest, where leaves are considered as patches for both fungal and bacterial communities. We determined the diversity of fungal and bacterial endophytes using environmental DNA sequencing of 365 top‐canopy leaves,...
Tipo: Text Palavras-chave: Bacteria; Distribution; French Guiana; Fungi; Metabarcoding; Plant–pathogen interactions; Rainforest.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00606/71808/70296.pdf
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Abundancia, actividad y diversidad de las bacterias heterótrofas en el Golfo de Batabanó y su uso como indicadoras ambientales OceanDocs
Miravet Regalado, M.E..
Se caracterizó la abundancia, actividad y biodiversidad de las bacterias heterótrofas aeróbias en el Golfo de Batabanó en diferentes épocas del año, desde 1982 hasta el 2000 y su relación con algunos factores abióticos, con el fin de interpretar las variaciones que muestran estos parámetros microbiológicos ante los cambios ambientales de origen natural o antrópico. A escala espacial, las mayores concentraciones de bacterias heterótrofas (BH) en las aguas se registraron hacia la regiónW del golfo y las menores concentraciones hacia el E y el NE, en las aguas de la Ensenada de la Broa, relacionándose principalmente con la disponibilidad de materia orgánica lábil en las aguas y el efecto indirecto de la dirección y sentido principal de las corrientes marinas....
Tipo: Theses and Dissertations Palavras-chave: Bacteria; Biodiversity; Bacteria; Biodiversity; Http://aims.fao.org/aos/agrovoc/c_765; Http://aims.fao.org/aos/agrovoc/c_33949.
Ano: 2003 URL: http://hdl.handle.net/1834/3398
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Actividad biológica en bacterias heterótrofas procedentes de "Bahía de La Habana". OceanDocs
Ortiz, E.; Morales, M.; Cartaya, Y.; Miranda, A.; Fonseca, E.; Paneque, K.; Caballero, V.; Martínez, C.; Díaz, Y..
En el presente trabajo se evaluó la actividad biológica de 87 aislamientos de bacterias heterótrofas procedentes de la "Bahía de La Habana". El espectro de potencialidades mostró que el 29,9% de los mismos presentó actividad antimicrobiana frente a las cepas indicadoras empleadas. Un 63,2% presentó actividad caseinasa, el 44,8% mostró su actividad hemolítica y el 83,4% fue capaz de excretar compuestos con actividad superficial. Respecto a los mecanismos degradativos, el 57,5% de ellas, mostró preferencia por el petróleo como fuente de carbono y el 42,5% por los compuestos organoclorados. Se evidenció la amplia gama de actividades con Bioactividad de bacterias heterótrofas aplicación biotecnológica que poseen las bacterias heterótrofas aisladas de éste...
Tipo: Preprint Palavras-chave: Biological collections; Geographical isolation; Bacteria; Heterotrophic organisms; Bacteria; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2004 URL: http://hdl.handle.net/1834/3655
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Adaptive features of a bacteriolytic enzyme from the style of the mussel Choromytilus meridionalis in response to environmental fluctuations. ArchiMer
Seiderer, L; Robb, F.
The extracellular digestive apparatus of bivalve molluscs consists of a crystalline style containing diverse degradative enzymes which are released during rotation and dissolution of the style. This paper reviews the occurrence of a temperature-regulated bacteriolytic enzyme in the black mussel Choromytilus meridionalis , which is an inhabitant of coastal reefs on the south and south west coast of South Africa. This agent is unable to lyse Micrococcus luteus or Escherichia coli , both of which are susceptible to lysis by a true lysozyme. It appears to have a restricted spectrum of activity which includes both free-living and kelp-associated gram-negative bacteria. In addition, the level of activity varies dramatically according to the environmental water...
Tipo: Text Palavras-chave: Choromytilus meridionalis; Bivalvia; Digestive system; Bacteria; Upwelling; Environmental effects; Adaptations; Bacteriocides; Marine molluscs; Enzymatic activity.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-991.pdf
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Aislamiento y caracterización bioquímica de metabolitos producidos por rizobacterias que solubilizan fosfato. Colegio de Postgraduados
Paredes Mendoza, Marianela.
El empleo de bacterias solubilizadoras de fosfato (BSP) para la solubilización de distintas rocas fosfatadas y de otras fuentes de fósforo es una alternativa para incrementar la cantidad de P disponible para las plantas. Se aislaron 147 BSP de la raíz y rizoplano de diferentes plantas cultivadas. El medio de cultivo denominado Medio Mínimo para Solubilización de Fosfato Colegio de Postgraduados con MgCl (MMSFCP-Mg) fue el más eficiente, debido a que en medio sólido se observaron halos de solubilización de hasta 3.8 cm a los catorce días, y en medio líquido se determinaron hasta 380 mg mL-1 de fosfato soluble a los seis días. Veintisiete de las mejores cepas fueron seleccionadas con base en la eficiencia de solubilización de fosfato...
Palavras-chave: Acidos orgánicos; Fosfatasas; Solubilización de fosfato; Inmunodetección; Roca fosfórica; Bacterias solubilizadoras de fosfato; Enfófitas; Organic acids; Phosphatases; Phosphate solubilization; Immunodetection; Rock phosphate; Phosphate solubilizing; Bacteria; Endophyte; Doctorado; Edafología.
Ano: 2010 URL: http://hdl.handle.net/10521/245
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Alteromonas -подобные бактерии, выделенные из воды и беспозвоночных Черного моря, и их антагонистическая активность. IBSS Repository
Онищенко, О. М..
51 штамм Alteromonas-подобных бактерий, изолированных из воды и беспозвоночных Черного моря, а также 11 типовых штаммов родов Pseudoalteromonas, Alteromonas, Marinomonas, Shewanella исследованы по 97 фенотипическим признакам и сгруппированы в 8 фенонов методами нумерического анализа. Черноморские изоляты отнесены к видам Pseudoalteromonas nigrifaciens, P. citrea, P. aurantia, P. haloplanktis, P. undina. Спектр их антагонистической активности отличался на различных по составу питательных средах: на богатой среде "В" антагонизм наблюдался в отношении спороносных бактерий и Candida albicans, на синтетической среде "ВМ" - в отношении P. aeruginosa, и в меньшей степени - дрожжей рода Candida.
Tipo: Journal Contribution Palavras-chave: Bacteria; Bacteria; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2001 URL: http://repository.ibss.org.ua/dspace/handle/99011/561
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An abyssal mobilome: Viruses, plasmids and vesicles from deep-sea hydrothermal vents ArchiMer
Lossouarn, Julien; Dupont, Samuel; Gorlas, Aurore; Mercier, Coraline; Bienvenu, Nadege; Marguet, Evelyne; Forterre, Patrick; Geslin, Claire.
Mobile genetic elements (MGEs) such as viruses, plasmids, vesicles, gene transfer agents (GTAs), transposons and transpovirions, which collectively represent the mobilome, interact with cellular organisms from all three domains of life, including those thriving in the most extreme environments. While efforts have been made to better understand deep-sea vent microbial ecology, our knowledge of the mobilome associated with prokaryotes inhabiting deep-sea hydrothermal vents remains limited. Here we focus on the abyssal mobilome by reviewing accumulating data on viruses, plasmids and vesicles associated with thermophilic and hyperthermophilic Bacteria and Archaea present in deep-sea hydrothermal vents.
Tipo: Text Palavras-chave: Deep-sea hydrothermal vent; Bacteria; Archaea; (Hyper-)thermophiles; Mobilome.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00260/37145/36731.pdf
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Analyse bacteriologique de l'eau par mesure potentiometrique de la reduction de l'acide lipoique ArchiMer
Charriere, G; Jouenne, T; Junter, G.
A simple potentiometric technique to detect and enumerate the bio-indicators of water contamination is described. The technique consists in measuring the redox potential of a specific culture medium saturated with oxygen where bacteria are incubated in presence of lipoic acid (LA). These micro-organisms reduce LA to dihydrolipoic acid, giving rise to an important potential drop in the system. This potentiometric "signal" reveals the presence of active metabolic bacteria in the medium. The major assets of potentiometric measurements in presence of LA lies in the reliability and intelligibility of the results (the redox phenomenon being qualitatively and quantitatively studied), associated to a very simple technology. Futhermore, this technique is perfectly...
Tipo: Text Palavras-chave: Bacteria; Pollution indicators; Water quality; Microbial contamination; Indicator species; Pollution detection; Redox potential; Titration.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1007.pdf
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Antarctic Psychrophile Bacteria Screening for Oil Degradation and Their Degrading Characteristics. OceanDocs
Zheng, Zhou; Jin, Qing; Miao, Jinlai; Liu, Fangming.
Two hydrocarbon degrading bacteria NJ276 and NJ341 were screened from 385 Antarctic marine bacteria and their degrading characteristics were studied. Diesel oil as sole carbon source was used in this study. The results showed that the oil degradation rates of Antarctic psychrophile bacteria NJ276 and NJ341 were 23.47 % and 32.15 %, respectively, after 20 days culturation at 5 °C, and the rates were 43.95 % and 62.47 % respectively after 20 days culturation at 15 °C. The oil degradation abilities were enhanced remarkably with the increasing culture temperature. GC - MSs indicated the residual oil contained C15 - C21 7 alkyls after degradation by NJ276, and C16, C17 and C18 3 alkyls after degradation by NJ341. The 16S rDNA gene sequences homology and...
Tipo: Journal Contribution Palavras-chave: Hydrocarbons; Degradation; Bacteria.
Ano: 2008 URL: http://hdl.handle.net/1834/5879
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Antibacterial activity of different types of snake venom from the Viperidae family against Staphylococcus aureus Biological Sciences
Canhas, Isabela Nascimento; Heneine, Luiz Guilherme Dias; Fraga, Thaís; Assis, Débora Cristina Sampaio de; Borges, Márcia Helena; Chartone-Souza, Edmar; Nascimento, Andréa Maria Amaral.
Toxins and venoms produced by living organisms have exhibited a variety of biological activities against microorganisms. In this study, we tested seven snake venoms from the family Viperidae for antibacterial activity and the activities of reversal of antibiotic resistance and inhibition of biofilm formation against 22 clinical isolates of Staphylococcus aureus. Bothrops moojeni venom exhibited anti staphylococcal activity with the lowest mean value of minimum inhibitory concentration (MIC). Moreover, reversal of antibiotic resistance was observed for combinations of B. moojeni venom (½ x MIC) and norfloxacin or ampicillin (both ½ x MIC) for 86.4% and 50% of the isolates, respectively. B. moojeni venom alone at ½ MIC inhibited 90% of biofilm formation,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bothrops moojeni; Bacteria; Antibiotic-resistance; NorA efflux pump; Biofilm..
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33826
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Antibacterial activity of four mouthrinses containing triclosan against salivary Staphylococcus aureus BJM
Tanomaru,Juliane Maria Guerreiro; Nascimento,Andresa Piacezzi; Watanabe,Evandro; Matoba-Júnior,Fumio; Tanomaru-Filho,Mário; Ito,Izabel Yoko.
The maximum inhibitory dilution (MID) of triclosan-based mouthwashes against 28 Staphylococcus aureus strains was evaluated. Dilutions ranging from 1/10 to 1/655,360 were prepared. Strains were inoculated using a Steers multipoint inoculator. The MID was considered as the maximum dilution capable of inhibiting microorganism growth. The mouthwashes presented different MIDs.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microorganism; Bacteria; Mouthwash; Triclosan.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300030
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Antibacterial activity of plant extracts from Brazil against fish pathogenic bacteria BJM
Castro,S.B.R.; Leal,C.A.G.; Freire,F.R.; Carvalho,D.A.; Oliveira,D.F.; Figueiredo,H.C.P..
The aim of this work was to evaluate the antibacterial activity of Brazilian plants extracts against fish pathogenic bacteria. Forty six methanolic extracts were screened to identify their antibacterial properties against Streptococcus agalactiae, Flavobacterium columnare and Aeromonas hydrophila. Thirty one extracts showed antibacterial activity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant extracts; Bacteria; Fish.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400030
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Antifouling effect of bioactive compounds from marine sponge Acanthella elongata and different species of bacterial film on larval attachment of Balanus amphitrite (cirripedia, crustacea) BABT
Ganapiriya,Viswambaran; Maharajan,Athisuyambulingam; Kumarasamy,Pothiappan.
The antifouling activity of bioactive compounds from marine sponge Acanthella elongata (Dendy) and five species of bacterial biofilm were studied. Larvae of Balanus amphitrite (Cyprids and nauplii) were used to monitor the settlement inhibition and the extent to which inhibition was due to toxicity. The crude extract and partially purified fractions of A.elongata showed significant inhibition over the settlement individually, and with the interaction of bacterial species. No bacterial film stimulated the barnacle settlement. The high but variable levels of antifouling activity in combination with less amount of toxicity showed the potential of these metabolites in environmentally-friendly antifouling preparations.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofouling; Bacterial film; Bacteria; Barnacles; Cyprids; B. amphitrite.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300010
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Antimicrobial and enzymatic activity of anemophilous fungi of a public university in Brazil Anais da ABC (AABC)
SOBRAL,LAUREANA V.; MELO,KELLY N.; SOUZA,CLECIANA M.; SILVA,SÍLVIO F.; SILVA,GILVANIA L.R.; SILVA,ANDRESSA L.F.; WANDERLEY,KATHARINE A.A.; OLIVEIRA,IDJANE S.; CRUZ,ROBERTA.
ABSTRACT To the fungal microbiota the UFPE and biotechnological potential enzymatic and antimicrobial production. Air conditioned environments were sampled using a passive sedimentation technique, the air I ratio and the presence of aflatoxigenic strains evaluated for ANVISA. Icelles were to determine the enzymatic activity of lipase, amylase and protease metabolic liquids to determine antimicrobial activity. Diversity was observed in all CAV environments, CFU/m3 ranged from 14 to 290 and I/E ratio from 0.1 to 1.5. The of the fungal genera were: Aspergillus (50%), Penicillium (21%), Talaromyces (14%), Curvularia and Paecilomyces (7% each). Aspergillus sydowii (Bainier & Sartory) Thom & Church presented enzymatic activity and the Talaromyces...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Air quality; Bacteria; Enzymes; Filamentous fungi; Refrigerated environments.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502327
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