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Registros recuperados: 6
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Comparaison de methodes directes (microscopiques) et indirectes (par mise en culture) dans l'evaluation d'une pollution bacterienne d'origine fecale. Etude preliminaire ArchiMer
Munro, P; Bianchi, M.
Cells of two serotypes of Escherichia coli (O26B6 and 0111B4) were counted using epifluorescence and immunofluorescence microscopy (nonavalent serum) and plate count on specific medium. Values from indirect counts were 10 fold smaller than those from both microscopic counts. In a sample of sea water from an area receiving a fecal pollution, the highest number of bacteria was obtained by epifluorescence microscopy. In that case, the number of cells counted by immunofluorescence microscopy was 10 fold smaller than the number of bacteria able to grow on the medium. It could be due to: the diversity of the natural community, the possibility to other bacteria than E. coli to grow on the used culture medium, the restrictiveness of the serum for "pathogenic...
Tipo: Text Palavras-chave: Escherichia coli; Bacteria; Microbiological culture; Pathogenic bacteria; Microbial contamination; Bacterial counters; Fluorescence microscopy; Bacteria collecting devices; Indicator species.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1001.pdf
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Determination du profil des flores bacteriennes des zones littorales. Application a l'evaluation de l'impact des rejets ArchiMer
Bunetel, L; Segui, A; Guerin, J; Lerman, I; Cormier, M.
The principle of this method is to evaluate, by counts exercised upon selective media, the relative importance of the different bacteria families in the flora of the coastal waters. It is important to separate the contaminant species and the commensal species in the marine environment. Ten culture media distributed into four categories (selective, non selective, saline and non saline) were examined by numeration. Counts were transferred on a diagram allowing to plot down a profile of bacterial flora of water sampled from a given geographical area. The profiles are arranged by a classification method. The sampling points with similar or adjoining profiles were assembled and marked on the chart. In this way, the authors obtained areas representing the impact...
Tipo: Text Palavras-chave: Bacteria; Coastal waters; Microbiological culture; Sewage disposal; Microbial contamination; Bacterial counters; Bacteria collecting devices.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1004.pdf
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Fluorescent antibody enumeration of Vibrio cholerae in the marine environment. ArchiMer
Brayton, P; Roszak, D; Palmer, L; Huq, S; Gimes, D.
From microcosm studies, V. cholerae and related potential human pathogens enter a viable but nonculturable state. Direct viable counts by epifluorescent microscopy consistently remain higher than corresponding plate counts. Thus, the assumption that pathogens "die-off" or "decay" in the marine environment must be re-evaluated, since stressed or nutrient-starved cells are unable to grow and be enumerated by standard plate count methods. Indirect immunofluorescent microscopy offers a more sensitive detection system for environment sampling for human pathogens. Field indicate that cultures positive for V. cholerae O1 are also positive by fluorescent antibody staining; however, the reverse was not necessarily true. By adapting epifluorescent techniques for...
Tipo: Text Palavras-chave: Vibrio cholerae; Bacteria; Experimental research; Marine environment; Pathogenic bacteria; Microbial contamination; Fluorescence microscopy; Bacterial counters; Pollution detection.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1000.pdf
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Mise en evidence et evaluation de l'enrichissement bacterien du film de surface des eaux littorales d'effluents urbains ArchiMer
Beucher, M; Plusquellec, A; Legal, Y; Cleret, J.
Sea water samples were collected in the vicinity of urban non-treated, urban treated and industrial outfalls. The samples belonged to two differents types: the first one consisted of the surface film, the second was the corresponding water at 50 cm depth. The enumeration of the bacteria in both types of samples indicated a significant concentration of the mesophilic flora and of the indicators of fecal pollution in the surface film. The value of the concentrating factor depended largely on the nature of the sewage and did not reflect differences between the various bacterial groups. At one of the stations a very significant regression was demonstrated between surface sample and depth sample.
Tipo: Text Palavras-chave: Bacteria; Bacteriology; Bacterial counters; Industrial wastes; Pollution indicators; Coastal waters; Microbial contamination; Indicator species; Sewage; Outfalls; Surface films.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1006.pdf
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Sampling for a bacteriological study of aquatic enrironments ArchiMer
Troussellier, M; Baleux, B; Andre, P.
Using water samples from several observation levels of a brackish lagoon, statistical solutions are offered for the sampling optimisation of bacteria viable counts. The variability between plate count is studied by the mean of the Taylor's power law. The sampling strategy optimisation, including several sources of variability, is realized by hierarchical analysis of variance and the estimation of the sampling cost of each source. This optimisation procedure results in a great increase of the relative efficiency of the sampling design and allow to obtain significant differences between the bacterial concentrations. The effect of the reduction of the total sampling effort on the precision of these average concentrations pointed out the scientific and...
Tipo: Text Palavras-chave: Bacteria; Abundance; Variance analysis; Microbiological analysis; Lagoons; Brackishwater environment; Bacterial counters; Biological sampling.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1984/acte-941.pdf
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The dispersion of adhered marine bacteria by pyrophosphate and ultrasound prior to direct counting. ArchiMer
Velji, M; Albright, L.
A technique has been investigated for dispersing adhered marine bacteria from various surfaces prior to direct counting. The bacterial cells were initially preserved and strengthened by use of 3.7% formaldehyde (v/v final concentration). Seawater samples were then treated with 0.001 M of tetrasodium pyrophosphate (final concentration), a sequestering and deflocculating agent. Surface sediment and kelp disc samples were treated with 0.01 M tetrasodium pyrophosphate (final concentration) after having been suspended in 0.44 M sodium chloride solution. These samples were incubated for 15-30 min. Subsequently, the samples were treated with ultrasound (100 W) for 30 to 45 s. This technique resulted in an even dispersion of most bacterial cells in the samples....
Tipo: Text Palavras-chave: Bacteria; Microbiological analysis; Flocculation; Ultrasonics; Bacterial counters.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-968.pdf
Registros recuperados: 6
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