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| Jabari,Linda; Gannoun,Hana; Khelifi,Eltaief; Cayol,Jean-Luc; Godon,Jean-Jacques; Hamdi,Moktar; Fardeau,Marie-Laure. |
| Abstract Wastewater from an anaerobic treatment plant at a slaughterhouse was analysed to determine the bacterial biodiversity present. Molecular analysis of the anaerobic sludge obtained from the treatment plant showed significant diversity, as 27 different phyla were identified. Firmicutes, Proteobacteria, Bacteroidetes, Thermotogae, Euryarchaeota (methanogens), and msbl6 (candidate division) were the dominant phyla of the anaerobic treatment plant and represented 21.7%, 18.5%, 11.5%, 9.4%, 8.9%, and 8.8% of the total bacteria identified, respectively. The dominant bacteria isolated were Clostridium, Bacteroides, Desulfobulbus, Desulfomicrobium, Desulfovibrio and Desulfotomaculum. Our results revealed the presence of new species, genera and families of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial ecology; Biodiversity; Bacterial culture; SSCP; PCR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100073 |
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| Peixoto,Rodolfo de Moraes; Araújo,Renata de Moraes Peixoto; Peixoto,Luciana Jatobá e Silva; Reges,Ariel Marques; Alves,Ana Paula Pereira; Pinheiro Júnior,José Wilton; Mota,Rinaldo Aparecido; Azevedo,Sérgio Santos; Costa,Mateus Matiuzzi da. |
| ABSTRACT: The aim of the present study was to assess two diagnostic techniques (California mastitis test (CMT) and the somatic cell count (SCC)) that can diagnose mastitis in dairy goats. Experimental infection was conducted using 20 mammary glands, a strain of Staphylococcus aureus, an infectious dose of 1.2x108CFU mL-1 and a volume of 1mL per mammary gland. The CMT and the SCC were used to detect subclinical mastitis. Bacterial culture (BC) was performed immediately after milk collection and was used as the gold standard. Four experimental time points were established (0, 24, 48 and 72 hours post-inoculation). Analysis of the ROC curve confirmed that the best combination of sensitivity and specificity were obtained with a cutoff point of 405.5, 6030.0... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial culture; Intramammary infection; Small ruminant; Somatic cell count. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701217 |
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