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Antioxidant activity of Agaricus brasiliensis basidiocarps on different maturation phases BJM
Mourão,Francielly; Umeo,Suzana Harue; Takemura,Orlando Seiko; Linde,Giani Andrea; Colauto,Nelson Barros.
Different maturation phases of basidiocarp could affect the bioactivity and concentration of some active substances. A. brasiliensis Wasser et al.(A. blazei Murrill) has shown antitumor activity that could be related to the antioxidant activity. However there is no information of the best basidiocarp maturation phase for extracting antioxidant substances in order to determine the moment of harvesting in mushroom cultivation. The objective of this work was to evaluate the antioxidant activity of A. brasiliensis strains on different basidiocarp maturation phases. The best condition for extraction of A. brasiliensis antioxidants is with methanol as solvent at 60 ºC for 60 min. Strains with closed basidiocarp have higher antioxidant activity than with opened...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Absorbance; Antioxidant activity; Basidiomycete; Agaricus blazei; DPPH.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100024
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Caracterização morfofisiológica e patogênica de isolados de Armillaria na região Sul do Brasil. Repositório Alice
SILVA, F. B.; MAZAROTTO, E. J.; SANTOS, A. F. dos; AUER, C. G..
Armillaria sp. é patógeno causador de podridão de raízes e a morte de árvores de Pinus na região Sul do Brasil. Pouco se conhece sobre aspectos morfofisiológicos e patogênicos dessa espécie. Este trabalho objetivou caracterizar a morfologia, fisiologia, patogenicidade e produção de basidiomas in vitro de isolados de Armillaria da região Sul do Brasil. A análise da morfologia do micélio e rizomorfa foi feita em colônias cultivadas em meio BDA (batatadextrose- ágar) a 20 ºC, no escuro, por 30 dias e em fragmentos de placas miceliais produzidas em seções de tronco inoculadas com o fungo, com microscopia de luz e de varredura. A fisiologia abordou a análise da biomassa produzida em caldo BD (batata-dextrose) em seis temperaturas. O teste de patogenicidade foi...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Fungo patogênico; Basidiomycete; Pathogenic fungus; Basidiomicetos; Armillaria; Basidiomycota; Pinus; Plant pathogenic fungi.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1090328
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Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium Biol. Res.
POLANCO,RUBÉN; CANESSA,PAULO; RIVAS,ALEXIS; LARRONDO,LUIS F; LOBOS,SERGIO; VICUÑA,RAFAEL.
In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1...
Tipo: Journal article Palavras-chave: Acel; Basidiomycete; Copper; Phanerochaete chrysosporium.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500007
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Exopolysaccharide from Agaricus brasiliensis LPB and its Scale Up Studies in a Stirred Tank Fermenter. Repositório Alice
GERN, J. C.; SANTOS, L. F. dos; HABU, S.; NOSEDA, M. D.; LIMA, L. F. O. de; SILVA, A. L. L. da; SOCCOL, V. T.; BRANDAO, H. de M.; SOCCOL, C. R..
Background and purpose: Although the activities of polysaccharides such as beta-glucans are well known in Agaricus brasiliensis, there has been little research on their exopolysaccharides and its characterization or scale up studies. The aim of the study was to study the monosaccharide composition of the exopolysaccharide (EPS) produced by Agaricus brasiliensis LPB3 and its production in a stirred tank fermenter. Materials and methods: Agaricus brasiliensis LPB3 was cultured in submerged fermentation in a medium containing glucose, yeast extract, K2HPO4 and MgSO4. Extracellular polysaccharide was precipitated with four volumes of 95% ethanol. For monosaccharide composition, the EPSs were hydrolyzed, derivatized to alditol acetates, and analyzed by gas...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Exopolysaccharide; Agaricus brasiliensis; Scale up; Monosaccharide composition; Stirred tank fermenter; Basidiomycete.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1037830
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Genetic characterization of isolates of the basidiomycete Agaricus blazei by RAPD BJM
Colauto,Nelson Barros; Dias,Eustáquio Souza; Gimenes,Marcos Aparecido; Eira,Augusto Ferreira da.
The genetic divergence of five isolates of Agaricus blazei was determined based on RAPD data. Results indicate that there is little genetic variability among the commercialized strains and that RAPD is a feasible and low cost technique that can be used to characterize this fungus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agaricus blazei; Basidiomycete; Genetic diversity; Mushroom; RAPD.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200006
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Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae) Neotropical Entomology
Silva-Pinhati,Ana C.O.; Bacci Jr.,Maurício; Siqueira,Célia G.; Silva,Aline; Pagnocca,Fernando C.; Bueno,Odair C.; Hebling,Maria J.A..
The isolation and maintenance of symbiotic basidiomycete fungi living in association with ants of the tribe Attini has been hindered by the slow growth rate of these fungi and the presence of other microorganisms on the surface of the material which the ants maintain inside their nests to provide a growth substrate for their symbiont. In this paper we describe a method which increases the efficiency of isolation of these fungal symbionts by over seven fold as compared to traditional isolation procedures. Underground nests of attine ants of the genera Atta, Acromyrmex, Trachymyrmex and Mycetarotes were located, from which samples containing the fungal symbiont and ants were collected and transported to the laboratory where the ants were able to clean the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atta; Acromyrmex; Trachymyrmex; Mycetarotes; Basidiomycete; Symbiosis.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100001
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Isolation and molecular characterization of symbiotic fungus from Acromyrmex ambiguus and Acromyrmex heyeri ants of Rio Grande do Sul State, Brazil Ciência Rural
Pereira,Daniela Isabel Brayer; Botton,Sônia de Avila; Azevedo,Maria Isabel de; Monteiro,Danieli Urach; Weiblen,Carla; Machado,Vanessa Schopf; Donatti,Michele Guimarães; Loeck,Alci Enimar.
Leaf-cutting ants of the genera Atta and Acromyrmex determine serious agricultural problems and live on symbiosis with Leucoagaricus gongylophorus. The aim of this study is to identify morphological and molecularly, as well as to verify the genotypic variability of the symbiotic fungus cultivated by A. heyeri and A. ambiguus from three different regions of the state of Rio Grande do Sul, Brazil. Fungus gardens were collected and fragments of mycelia were grown in selective medium. Total DNA was extracted and amplification of the ITS region was performed by PCR using universal primers. After DNA sequencing, the chromatograms were assembled and phylogenetic analyzes were performed by the Neighbor-Joining method. A total of six isolates of L. gongylophorus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Basidiomycete; Leucoagaricus gongylophorus; Internal transcribed spacer..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701256
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Selection of aggressive pathogenic and solopathogenic strains of Ustilago maydis to improve Huitlacoche production Acta Botanica
Galicia-García,Porfirio Raúl; Silva-Rojas,Hilda Victoria; Mendoza-Onofre,Leopoldo Ernesto; Zavaleta-Mancera,Hilda Araceli; Córdova-Téllez,Leobigildo; Espinosa-Calderón,Alejandro.
ABSTRACT Ustilago maydis is a basidiomycete known as the causative agent of 'common smut', worldwide disease of maize that is recognized by the galls it forms, which have considerable potential as a gourmet food. Results of infection are quite variable, even under optimal greenhouse conditions. In order to find pathogenic strains able to be used as a highly infective and stable inoculum for the successful production of galls either in greenhouses or in the field, ears with gall symptoms containing teliospores were recovered from maize plants. The teliospores were suspended in water and plated on nutrient-rich medium. Twenty-six colonies developed, containing three types of yeast-like colonies: saprotrophic, pathogenic, and solopathogenic. DAPI staining...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Basidiomycete; Maize galls; Mating types; Molecular diagnosis; Smut.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000400683
Registros recuperados: 8
Primeira ... 1 ... Última
 

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