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Anthracycline production by Streptomyces capoamus in batch fermentation BABT
Martins,Cristiane Soares; Souto-Maior,Ana Maria.
Anthracyclines have been intensely studied worldwide due to their importance as anticancer agents. In this work investigation was made on the production of ciclamycin, an antitumour complex of the anthracycline class, produced by Streptomyces capoamus. The fermentation medium consisted of glucose, as the main carbon source, and soymilk, as the sole nitrogen source. Experiments were performed in a 5-liter batch bioreactor under nitrogen and carbon-limiting conditions. Increasing antibiotic activity was observed both intra and extracellularly during growth under the two conditions used. Progressive loss of activity occurred in both cases after glucose was depleted from the medium. The results obtained showed that harvest of ciclamycin in a batch process...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthracycline; Ciclamycin; Streptomyces capoamus; Batch fermentation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300021
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Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus BABT
Cazetta,Marcia Luciana; Monti,Rubens; Contiero,Jonas.
The present study was conducted to investigate the influence of initial sucrose concentration, pH and aeration rate on biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus in a stirred batch reactor. Maximum inulinase activity (15.29 UmL-1) was obtained at a sucrose concentration of 10 g L-1, pH 5.0 and aeration rate of 1 vvm. The 20 g L-1 sucrose concentration was suitable for cell growth; however, enzymatic activity at this concentration was inhibited due to catabolic repression. The increase in aeration rate caused a reduction in enzyme activity with no relevant biomass increase.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kluyveromyces marxianus var. bulgaricus; Sucrose; Inulinase; Biomass; Batch fermentation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300027
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Glutathione production using magnetic fields generated by magnets BABT
Santos,Lucielen Oliveira dos; Gonzales,Tatiane Araujo; Úbeda,Beatriz Torsani; Alegre,Ranulfo Monte.
The objective of this work was to study the production of GSH by Saccharomyces cerevisiae ATCC 7754 in a fermentor (5 L) using a cell recycle system with magnets. The fermentation conditions were 20°C, 500 rpm, 5% (v/v) of inoculum, pHinitial 5, 1.1 vvm aeration and total fermentation time of 72 h. The time of application of MF ranged from 24, 48 or 72 h. In comparison to the control experiment, the best results were obtained with 72 h of application of MF. The cell concentration reached 19.5 g/L and GSH concentration was 271.9 mg/L that corresponded to an increase of 2.63 and 32.1% compared to the control experiment, respectively.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutathione; Magnetic field; Saccharomyces cerevisiae; Batch fermentation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600016
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Influence of medium constituents on the biosynthesis of cephalosporin-C Electron. J. Biotechnol.
Kumar Nigam,Vinod; Verma,Ruchi; Kumar,Abhishek; Kundu,Subir; Ghosh,Purnendu.
Acremonium chrysogenum NCIM 1069 was used for the biosynthesis of cephalosporin-C (CPC) in batch mode of cultivation. The effect of different medium constituents for better yield of CPC was thoroughly investigated. From the results of the fermentation, it was found that ammonium sulphate as inorganic nitrogen source and methionine at the concentration of 0.4% are most suitable for higher yield of antibiotic. The variation in the C/N ratio on the biosynthesis of CPC showed that a C/N ratio of 8.0 is most suitable for maximum production of CPC
Tipo: Journal article Palavras-chave: Acremonium chrysogenum; Batch fermentation; Cephalosporin-C; Methionine; Stirred tank bioreactor.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200007
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