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Print-capture PCR for detection of tomato begomoviruses from plants and whiteflies Trop. Plant Pathol.
Nagata,Tatsuya; Inoue-Nagata,Alice K; Ávila,Antonio C. de; Giordano,Leonardo de B.
Print-capture (PC) Polymerase chain reaction (PCR) was evaluated as a novel detection method of plant viruses. Tomato (Lycopersicon esculentum) plants infected with begomovirus (fam. Geminiviridae, gen. Begomovirus) and viruliferous whiteflies were used to study the efficiency of the method. Print-capturing steps were carried out using non-charged nylon membrane or filter paper as the solid support for DNA printings. Amplified DNA fragments of expected size were consistently obtained by PCR from infected plants grown in a greenhouse, after direct application of printed materials to the PCR mix. However, virus detection from a single whitefly and from field-grown tomato samples required a high temperature treatment of printed material prior to PCR...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Geminivírus; Bemisia tabaci; Bemisia argentifoli.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000100014
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