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Registros recuperados: 10
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Antimicrobial Susceptibility and Biofilm Production by Salmonella sp. Strains Isolated from Frozen Poultry Carcasses Rev. Bras. Ciênc. Avic.
Sereno,MJ; Ziech,RE; Druziani,JT; Pereira,JG; Bersot,LS.
ABSTRACT The objectives of this study were to evaluate the antimicrobial resistance and the biofilm-producing ability of Salmonella sp. strains isolated from frozen poultry carcasses. Antimicrobial susceptibility was tested by the disk-diffusion method. Biofilm-producing ability was determined in 96-well polystyrene microplates stained with crystal violet at 1%. Out of the 22 strains tested, all were multiresistant, that is, resistant to more than three antimicrobial classes, and 72.7% were able to form biofilms. The highest resistance rates obtained were against sulfonamides, tetracycline, and quinolones. On the other hand, 100% of the strains were sensitive to chloramphenicol. According to the rate of biofilm formation, 3 (13.6%) and 13 (59.1%) strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm formation; Food pathogens; Multidrug-resistance; Poultry meat.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100103
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Biofilm formation and binding specificities of CFA/I, CFA/II and CS2 adhesions of Enterotoxigenic Escherichia coli and CfaE-R181A mutant BJM
Liaqat,Iram.
Enterotoxigenic Escherichia coli (ETEC) strains are leading causes of childhood diarrhea in developing countries. Adhesion is the first step in pathogenesis of ETEC infections and ETEC pili designated colonization factor antigens (CFAs) are believed to be important in the biofim formation, colonization and host cell adhesions. As a first step, we have determined the biofilm capability of ETEC expressing various types of pili (CFA/I, CfaE-R181A mutant/ CfaE tip mutant, CFA/II and CS2). Further, enzyme-linked immunosorbent assay (ELISA) assay were developed to compare the binding specificity of CFA/I, CFA/II (CS1 - CS3) and CS2 of ETEC, using extracted pili and piliated bacteria. CFA/II strain (E24377a) as well as extracted pili exhibited significantly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ETEC; CFA/I; CFA/II; CS2; Biofilm formation; ELISA assays; Asialo-GM1; R181-cotD; Dsc19CotD(His)6.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300018
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Effects of different osmolarities on bacterial biofilm formation BJM
Kavamura,Vanessa Nessner; Melo,Itamar Soares de.
Biofilm formation depends on several factors. The influence of different osmolarities on bacterial biofilm formation was studied. Two strains (Enterobacter sp. and Stenotrophomonas sp.) exhibited the most remarkable alterations. Biofilm formation is an important trait and its use has been associated to the protection of organisms against environmental stresses.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria; Biofilm formation; NaCl; D-sorbitol.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200034
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Effects of sub-minimum inhibitory concentrations of ciprofloxacin on biofilm formation and virulence factors of Escherichia coli BJID
Dong,Guofeng; Li,Jiahui; Chen,Lijiang; Bi,Wenzi; Zhang,Xiaoxiao; Liu,Haiyang; Zhi,Xiaoyang; Zhou,Tieli; Cao,Jianming.
ABSTRACT Objective: To evaluate the influence of sub-minimum inhibitory concentrations (MICs) of ciprofloxacin (CIP) on biofilm formation and virulence factors of Escherichia coli clinical isolates. Methods: Sub-MICs of CIP were determined using growth curve experiments. The biofilm-forming capacity of E. coli clinical isolates and E. coli ATCC 25922 treated or untreated with sub-MICs of CIP was assessed using a crystal violet staining assay. The biofilm structure of E. coli isolate was assessed with scanning electron microscopy (SEM). The expression levels of the virulence genes fim, usp, and iron and the biofilm formation genes of the pgaABCD locus were measured using quantification RT-PCR (qRT-PCR) in E. coli isolates and E. coli ATCC 25922. Results:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm formation; Ciprofloxacin; Escherichia coli; Sub-minimum inhibitory concentration; Virulence.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100015
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Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk BJM
Martins,Maurilio L.; Uelinton,M. Pinto; Riedel,Kathrin; Vanetti,Maria C.D.; Mantovani,Hilário C.; Araújo,Elza F. de.
Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs), which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Milk; Pseudomonas fluorescens; Biofilm formation; Quorum sensing; Quorum quenching.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300037
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Lactobacillus acidophilus-derived biosurfactant effect on gtfB and gtfC expression level in Streptococcus mutans biofilm cells BJM
Tahmourespour,Arezoo; Salehi,Rasoul; Kermanshahi,Rooha Kasra.
Streptococcus mutans (S. mutans), harboring biofilm formation, considered as a main aetiological factor of dental caries. Gtf genes play an important role in S. mutans biofilm formation. The purpose of this study was to investigate the effect of Lactobacillus acidophilus-derived biosurfactant on S. mutans biofilm formation and gtfB/C expression level (S. mutans standard strain ATCC35668 and isolated S. mutans strain (22) from dental plaque). The Lactobacillus acidophilus (L. acidophilus) DSM 20079 was selected as a probiotic strain to produce biosurfactant. The FTIR analysis of its biosurfactant showed that it appears to have a protein-like component. Due to the release of such biosurfactants, L. acidophilus was able to interfere in the adhesion and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm formation; Biosurfactant; GtfB/C; Lactobacillus acidophilus; Real-time RT PCR; Streptococcus mutans.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100042
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Molecular detection of virulence factors among food and clinical Enterococcus faecalis strains in South Brazil BJM
Medeiros,A.W.; Pereira,R.I.; Oliveira,D.V.; Martins,P.D.; d'Azevedo,P.A.; Van der Sand,S.; Frazzon,J.; Frazzon,A.P.G.
The present report aimed to perform a molecular epidemiological survey by investigating the presence of virulence factors in E. faecalis isolated from different human clinical (n = 57) and food samples (n = 55) in Porto Alegre, Brazil, collected from 2006 to 2009. In addition, the ability to form biofilm in vitro on polystyrene and the β-haemolytic and gelatinase activities were determined. Clinical strains presented a higher prevalence of aggregation substance (agg), enterococcal surface protein (esp) and cytolysin (cylA) genes when compared with food isolates. The esp gene was found only in clinical strains. On the other hand, the gelatinase (gelE) and adherence factor (ace) genes had similar prevalence among the strains, showing the widespread...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Virulence determinants; Clinical enterococci; Food enterococci; Biofilm formation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100047
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Multi drug resistance in strong biofilm forming clinical isolates of Staphylococcus epidermidis BJM
Sahal,Gulcan; Bilkay,Isil Seyis.
Staphylococcus epidermidis which exists in healthy human skin as a commensal inhabitant is also an important pathogen forming biofilms on many surfaces and recently, increased resistance traits were suggested to be acquired in biofilm environments. In this study; clinical Prevalences, antibiotic resistances and biofilm formations of S. epidermidis strains were determined and comparison of all these findings with each other was carried out in order to take precautions against them and figure out if high biofilm forming S. epidermidis strains display multi drug resistance. According to our results; samples of wound and blood were the most S. epidermidis isolated clinical materials (40%; 35%) and cardiothoracic surgery was the most S. epidermidis observed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus epidermidis; Biofilm formation; Β-lactam resistance; Multi drug resistance; Indwelling medical device related infections.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200023
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Ostreopsis cf. ovata Bloom in Currais, Brazil: Phylogeny, Toxin Profile and Contamination of Mussels and Marine Plastic Litter ArchiMer
Tibiriçá, Carlos Eduardo J. A.; Leite, Isabel P.; Batista, Talita V. V.; Fernandes, Luciano F.; Chomérat, Nicolas; Herve, Fabienne; Hess, Philipp; Mafra, Luiz.
Ostreopsis cf. ovata is a toxic marine benthic dinoflagellate responsible for harmful blooms affecting ecosystem and human health, mostly in the Mediterranean Sea. In this study we report the occurrence of a summer O. cf. ovata bloom in Currais, a coastal archipelago located on the subtropical Brazilian coast (~25° S). This bloom was very similar to Mediterranean episodes in many aspects: (a) field-sampled and cultivated O. cf. ovata cells aligned phylogenetically (ITS and LSU regions) along with Mediterranean strains; (b) the bloom occurred at increasing temperature and irradiance, and decreasing wind speed; (c) cell densities reached up to 8.0 × 104 cell cm−2 on fiberglass screen and 5.6 × 105 cell g−1 fresh weight on seaweeds; (d) and toxin profiles...
Tipo: Text Palavras-chave: Harmful algal bloom; Benthic microalgae; Toxic dinoflagellates; Ovatoxin; Toxin transfer; Seafood safety; Marine pollution; Plastic litter; Biofilm formation.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00509/62037/66177.pdf
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Screening of Lactobacillus spp. for the prevention of Pseudomonas aeruginosa pulmonary infections ArchiMer
Alexandre, Youenn; Le Berre, Rozenn; Barbier, Georges; Le Blay, Gwenaelle.
Background: Pseudomonas aeruginosa is an opportunistic pathogen that significantly increases morbidity and mortality in nosocomial infections and cystic fibrosis patients. Its pathogenicity especially relies on the production of virulence factors or resistances to many antibiotics. Since multiplication of antibiotic resistance can lead to therapeutic impasses, it becomes necessary to develop new tools for fighting P. aeruginosa infections. The use of probiotics is one of the ways currently being explored. Probiotics are microorganisms that exert a positive effect on the host's health and some of them are known to possess antibacterial activities. Since most of their effects have been shown in the digestive tract, experimental data compatible with the...
Tipo: Text Palavras-chave: Pseudomonas aeruginosa; Lactobacillus; Probiotics; Organic acids; Biofilm formation; Elastolytic activity.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00197/30842/29211.pdf
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