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| Registros recuperados: 10 | |
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| Sereno,MJ; Ziech,RE; Druziani,JT; Pereira,JG; Bersot,LS. |
| ABSTRACT The objectives of this study were to evaluate the antimicrobial resistance and the biofilm-producing ability of Salmonella sp. strains isolated from frozen poultry carcasses. Antimicrobial susceptibility was tested by the disk-diffusion method. Biofilm-producing ability was determined in 96-well polystyrene microplates stained with crystal violet at 1%. Out of the 22 strains tested, all were multiresistant, that is, resistant to more than three antimicrobial classes, and 72.7% were able to form biofilms. The highest resistance rates obtained were against sulfonamides, tetracycline, and quinolones. On the other hand, 100% of the strains were sensitive to chloramphenicol. According to the rate of biofilm formation, 3 (13.6%) and 13 (59.1%) strains... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilm formation; Food pathogens; Multidrug-resistance; Poultry meat. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100103 |
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| Liaqat,Iram. |
| Enterotoxigenic Escherichia coli (ETEC) strains are leading causes of childhood diarrhea in developing countries. Adhesion is the first step in pathogenesis of ETEC infections and ETEC pili designated colonization factor antigens (CFAs) are believed to be important in the biofim formation, colonization and host cell adhesions. As a first step, we have determined the biofilm capability of ETEC expressing various types of pili (CFA/I, CfaE-R181A mutant/ CfaE tip mutant, CFA/II and CS2). Further, enzyme-linked immunosorbent assay (ELISA) assay were developed to compare the binding specificity of CFA/I, CFA/II (CS1 - CS3) and CS2 of ETEC, using extracted pili and piliated bacteria. CFA/II strain (E24377a) as well as extracted pili exhibited significantly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ETEC; CFA/I; CFA/II; CS2; Biofilm formation; ELISA assays; Asialo-GM1; R181-cotD; Dsc19CotD(His)6. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300018 |
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| Dong,Guofeng; Li,Jiahui; Chen,Lijiang; Bi,Wenzi; Zhang,Xiaoxiao; Liu,Haiyang; Zhi,Xiaoyang; Zhou,Tieli; Cao,Jianming. |
| ABSTRACT Objective: To evaluate the influence of sub-minimum inhibitory concentrations (MICs) of ciprofloxacin (CIP) on biofilm formation and virulence factors of Escherichia coli clinical isolates. Methods: Sub-MICs of CIP were determined using growth curve experiments. The biofilm-forming capacity of E. coli clinical isolates and E. coli ATCC 25922 treated or untreated with sub-MICs of CIP was assessed using a crystal violet staining assay. The biofilm structure of E. coli isolate was assessed with scanning electron microscopy (SEM). The expression levels of the virulence genes fim, usp, and iron and the biofilm formation genes of the pgaABCD locus were measured using quantification RT-PCR (qRT-PCR) in E. coli isolates and E. coli ATCC 25922. Results:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilm formation; Ciprofloxacin; Escherichia coli; Sub-minimum inhibitory concentration; Virulence. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100015 |
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| Martins,Maurilio L.; Uelinton,M. Pinto; Riedel,Kathrin; Vanetti,Maria C.D.; Mantovani,Hilário C.; Araújo,Elza F. de. |
| Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs), which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Milk; Pseudomonas fluorescens; Biofilm formation; Quorum sensing; Quorum quenching. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300037 |
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| Medeiros,A.W.; Pereira,R.I.; Oliveira,D.V.; Martins,P.D.; d'Azevedo,P.A.; Van der Sand,S.; Frazzon,J.; Frazzon,A.P.G. |
| The present report aimed to perform a molecular epidemiological survey by investigating the presence of virulence factors in E. faecalis isolated from different human clinical (n = 57) and food samples (n = 55) in Porto Alegre, Brazil, collected from 2006 to 2009. In addition, the ability to form biofilm in vitro on polystyrene and the β-haemolytic and gelatinase activities were determined. Clinical strains presented a higher prevalence of aggregation substance (agg), enterococcal surface protein (esp) and cytolysin (cylA) genes when compared with food isolates. The esp gene was found only in clinical strains. On the other hand, the gelatinase (gelE) and adherence factor (ace) genes had similar prevalence among the strains, showing the widespread... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Virulence determinants; Clinical enterococci; Food enterococci; Biofilm formation. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100047 |
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| Tibiriçá, Carlos Eduardo J. A.; Leite, Isabel P.; Batista, Talita V. V.; Fernandes, Luciano F.; Chomérat, Nicolas; Herve, Fabienne; Hess, Philipp; Mafra, Luiz. |
| Ostreopsis cf. ovata is a toxic marine benthic dinoflagellate responsible for harmful blooms affecting ecosystem and human health, mostly in the Mediterranean Sea. In this study we report the occurrence of a summer O. cf. ovata bloom in Currais, a coastal archipelago located on the subtropical Brazilian coast (~25° S). This bloom was very similar to Mediterranean episodes in many aspects: (a) field-sampled and cultivated O. cf. ovata cells aligned phylogenetically (ITS and LSU regions) along with Mediterranean strains; (b) the bloom occurred at increasing temperature and irradiance, and decreasing wind speed; (c) cell densities reached up to 8.0 × 104 cell cm−2 on fiberglass screen and 5.6 × 105 cell g−1 fresh weight on seaweeds; (d) and toxin profiles... |
| Tipo: Text |
Palavras-chave: Harmful algal bloom; Benthic microalgae; Toxic dinoflagellates; Ovatoxin; Toxin transfer; Seafood safety; Marine pollution; Plastic litter; Biofilm formation. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00509/62037/66177.pdf |
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| Registros recuperados: 10 | |
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