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| Registros recuperados: 28 | |
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| Iñiguez-Moreno,Maricarmen; Gutiérrez-Lomelí,Melesio; Guerrero-Medina,Pedro Javier; Avila-Novoa,María Guadalupe. |
| Abstract The aim of this study was evaluated the biofilm formation by Staphylococcus aureus 4E and Salmonella spp. under mono and dual-species biofilms, onto stainless steel 316 (SS) and polypropylene B (PP), and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite. The biofilms were developed by immersion of the surfaces in TSB by 10 d at 37 °C. The results showed that in monospecies biofilms the type of surface not affected the cellular density (p > 0.05). However, in dual-species biofilms on PP the adhesion of Salmonella spp. was favored, 7.61 ± 0.13 Log10 CFU/cm2, compared with monospecies biofilms onto the same surface, 5.91 ± 0.44 Log10 CFU/cm2 (p < 0.05). The mono and dual-species biofilms were subjected to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Salmonella spp.; Staphylococcus aureus; Disinfectants; Sensitivity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200310 |
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| Aparna,Madhu Sharma; Yadav,Sarita. |
| Bacteria that attach to surface aggregate in a hydrated polymeric matrix of their own synthesis to form biofilms. These represent microbial societies with their own defense and communication system. Transitioning from acute to chronic infection is frequently associated with biofilm formation.Bacteria in biofilms are innately more resistant to antimicrobial agents. The presence of indwelling medical devices increases the risk for biofilm formation and subsequent infection. The current antibiotic therapies are of limited effectiveness in resolving biofilms infection.This review attempts to discuss the stages in biofilm formation, their pathogenic mechanisms, effect of antimicrobial agents, detection and eradication of the biofilms. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Staphylococci; Pseudomonas; Candida. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600016 |
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| Melo,Poliana de Castro; Ferreira,Luciano Menezes; Nader Filho,Antônio; Zafalon,Luiz Francisco; Vicente,Hinig Isa Godoy; Souza,Viviane de. |
| Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Bovine mastitis; Phenotypic and molecular analysis; Staphylococcus aureus. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100017 |
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| Becker,Sidnei; Soares,Cíntia; Porto,Luismar Marques. |
| Groups of genes that produce exopolysaccharide with a N-acetyl-D-glucosamine monomer are in the genome of several pathogenic bacteria. Chromobacterium violaceum, an opportunistic pathogen, has the operon hmsHFR-CV2940, whose proteins can synthesize such polysaccharide. In this work, multiple alignments among proteins from bacteria that synthesize such polysaccharide were used to verify the existence of amino acids that might be critical for pathogen activity. Three-dimensional models were generated for spatial visualization of these amino acid residues. The analysis carried out showed that the protein HmsR preserves the amino acids D135, D228, Q264 and R267, considered critical for the formation of biofilms and, furthermore, that these amino acids are... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Exopolysaccharide; Chromobacterium violaceum pathogenicity; Comparative genomics. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300031 |
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| Tavares,Luciana Lopes; Almeida,Crislene Barbosa de; Caruso,Ícaro Putinhon; Cornélio,Marinônio Lopes; Lopes Filho,José Francisco. |
| The purpose of this study was to evaluate changes in the structure and some functional properties of biofilms added with modified clays (Cloisite® 15A and Cloisite® 30B) prepared by the casting method. The analysis of the microstructure of the films, scanning electron microscopy (SEM), Optical microscopy (MO), and Infrared Spectroscopy (FTIR) indicated that the addition of clay in the films resulted in the formation of a heterogeneous microstructure, microcomposite or tactoid. Due to the formation of a microcomposite structure, functional properties of the films added with both clays such as opacity, solubility, and permeability to water vapor (PVA), were not better than those of the control film. Thus, it was concluded that although it is possible to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Zein; Clays; Microcomposite; Tactoids. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000200017 |
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| Cândido,Cinthya S.; Portella,Cadmo Silton A.; Laranjeira,Bruno J.; Silva,Sérgio S. da; Arriaga,Angela M.C.; Santiago,Gilvandete M. P.; Gomes,Geovany A.; Almeida,Paulo César; Carvalho,Cibele B. M.. |
| The essential oil from the leaves of Myrcia ovata Cambess., commonly used in Brazil for the treatment of gastric illnesses, was screened for antimicrobial activity and action in the formation of microbial biofilms by Enterococcus faecalis. The oil was obtained by hydrodistillation using a clevenger-type system. Its chemical composition was analyzed using GC and GC-MS. Both MIC and MBC of the essential oil were determined by broth microdilution techniques and agar dilution method. The essential oil showed antimicrobial activity against E. faecalis, Escherichia coli, Pseudomonas aeruginosa, Salmonella choleraesuis, Staphylococcus aureus, Streptococcus pneumoniae and Candida parapsilosis. The results showed that the essential oil of M. ovata Cambess. was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Essential oil; Myrcia ovata Cambess.; Antimicrobial activity; Biofilms. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300012 |
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| Hassan,Afreenish; Usman,Javaid; Kaleem,Fatima; Omair,Maria; Khalid,Ali; Iqbal,Muhammad. |
| BACKGROUND: Microorganisms growing in a biofilm are associated with chronic and recurrent human infections and are highly resistant to antimicrobial agents. There are various methods to detect biofilm production like Tissue Culture Plate (TCP), Tube method (TM), Congo Red Agar method (CRA), bioluminescent assay, piezoelectric sensors, and fluorescent microscopic examination. OBJECTIVE: This study was conducted to compare three methods for the detection of biofilms. METHOD: The study was carried out at the Department of Microbiology, Army Medical College, National University of Sciences and Technology, Pakistan, from January 2010 to June 2010. A total of 110 clinical isolates were subjected to biofilm detection methods. Isolates were identified by standard... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Bacteria; Anti-bacterial agents. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400002 |
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| Mandelli,JZA; Ehrhardt,A; Manto,L; Borges,KA; Furian,TQ; Weber,B; Rodrigues,LB; Santos,LR. |
| ABSTRACT Antimicrobial resistance is a serious public health problem and Salmonella spp. is highly resistant to antimicrobial agents. Biofilms are important in the food industry due to their formation on products, utensils, and surfaces and the difficulty in their removal. The objective of this study was to assess extended spectrum beta-lactamase (ESBL) production, antimicrobial resistance, and biofilm production of Salmonella isolated from poultry slaughterhouses. Antimicrobial susceptibility was assessed by the disk diffusion assay and ESBL by double diffusion disk assay using the beta-lactamase inhibitor (amoxicillin+clavulanate). The antimicrobials tested were: ampicillin, amoxicillin+clavulanate, aztreonam, ceftazidime, cefotaxime, chloramphenicol,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Drug resistance; ESBL; Salmonella. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200348 |
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| Segura, Audrey; Auffret, Pauline; Bibbal, Delphine; Bertoni, Marine; Durand, Alexandra; Jubelin, Gregory; Kerouredan, Monique; Brugere, Hubert; Bertin, Yolande; Forano, Evelyne. |
| Healthy cattle are the primary reservoir for O157:H7 Shiga toxin-producing E coil responsible for human food-borne infections. Because farm environment acts as a source of cattle contamination, it is important to better understand the factors controlling the persistence of E. coil O157:H7 outside the bovine gut. The E. coil O157:H7 strain MC2, identified as a persistent strain in French farms, possessed the characteristics required to cause human infections and genetic markers associated with clinical O157:H7 isolates. Therefore, the capacity of E. coil MC2 to survive during its transit through the bovine gastro-intestinal tract (GIT) and to respond to stresses potentially encountered in extra intestinal environments was analyzed. E. coil MC2 survived in... |
| Tipo: Text |
Palavras-chave: Escherichia coli; STEC; EHEC; O157:H7; Biofilms; Stress response; Persistence. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00434/54588/55972.pdf |
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| Santos Villamil, Alfricia Adriana de los. |
| La presencia de Salmonella en nopal verdura fresco (Opuntia ficus-indica L.) MILL se ha documentado recientemente (Hernández et al., 2009). La contaminación de alimentos es un problema de salud pública que afecta a las personas en todo el mundo. Una característica importante de Salmonella es su capacidad para formar biopelículas, las cuales son comunidades de células microbianas embebidas en una matriz de exopolímeros que ellas mismas producen en respuesta a factores como la temperatura, la humedad relativa y la composición del medio en que se encuentran (Annous et al., 2005; Stepanovic et al., 2000). Esta capacidad le permite a Salmonella adherirse de manera irreversible y de colonizar superficies bióticas y abióticas (Annous et al., 2005, Stepanovic et... |
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Palavras-chave: Biopelículas; Desinfectantes; Nopal; Salmonella; Biofilms; Desinfectants; Maestría; Fitopatología. |
| Ano: 2010 |
URL: http://hdl.handle.net/10521/220 |
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| Santos Villamil, Alfricia Adriana de los. |
| La presencia de Salmonella en nopal verdura fresco (Opuntia ficus-indica L.) MILL se ha documentado recientemente (Hernández et al., 2009). La contaminación de alimentos es un problema de salud pública que afecta a las personas en todo el mundo. Una característica importante de Salmonella es su capacidad para formar biopelículas, las cuales son comunidades de células microbianas embebidas en una matriz de exopolímeros que ellas mismas producen en respuesta a factores como la temperatura, la humedad relativa y la composición del medio en que se encuentran (Annous et al., 2005; Stepanovic et al., 2000). Esta capacidad le permite a Salmonella adherirse de manera irreversible y de colonizar superficies bióticas y abióticas (Annous et al., 2005, Stepanovic et... |
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Palavras-chave: Biopelículas; Desinfectantes; Nopal; Salmonella; Biofilms; Desinfectants; Maestría; Fitopatología. |
| Ano: 2010 |
URL: http://hdl.handle.net/10521/220 |
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| Reis-Teixeira,Fernanda Barbosa dos; Alves,Virgínia Farias; Martinis,Elaine Cristina Pereira de. |
| Abstract The pathogenic bacterium Listeria monocytogenes can persist in food processing plants for many years, even when appropriate hygienic measures are in place, with potential for contaminating ready-to-eat products and, its ability to form biofilms on abiotic surfaces certainly contributes for the environmental persistence. In this research, L. monocytogenes was grown in biofilms up 8 days attached to stainless steel and glass surfaces, contributing for advancing the knowledge on architecture of mature biofilms, since many literature studies carried out on this topic considered only early stages of cell adhesion. In this study, biofilm populations of two strains of L. monocytogenes (serotypes 1/2a and 4b) on stainless steel coupons and glass were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Listeria monocytogenes; Abiotic surfaces; Persistence; Biofilms. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300587 |
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| Zeraik,Ana Eliza; Nitschke,Marcia. |
| Bacterial adhesion to inert surfaces is a complex process influenced by environmental conditions. In this work, the influence of growth medium and temperature on the adhesion of Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Micrococcus luteus and Listeria monocytogenes to polystyrene surfaces was studied. Most bacteria demonstrated the highest adhesion when cultured in TSYEA, except S. marcescens, which showed to be positively influenced by the pigment production, favored in poor nutrient media (lactose and peptone agar). P. aeruginosa adhesion to polystyrene increased at low temperatures whatever the medium used. The culture medium influenced the surface properties of the bacteria as assessed by the MATS test. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial adhesion; Biofilms; Hydrophobicity; Polystyrene. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400012 |
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| Registros recuperados: 28 | |
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