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Registros recuperados: 33
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A linear 19-Mer plant defensin-derived peptide acts synergistically with caspofungin against Candida albicans biofilms. Repositório Alice
COOLS, T. L.; STRUYFS, C.; DRIJFHOUT, J. W.; KUCHARÍKOVÁ, S.; ROMERO, C. L.; DIJCK, P. V.; RAMADA, M. H. S.; BLOCH JUNIOR, C.; CAMMUE, B. P. A.; THEVISSEN, K..
bitstream/item/169781/1/Defensin-2017-Frontiers-in-Mic.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Fungal infections; Biofilms; Candida; Antimicrobial peptides; Catheters.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1083484
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Antibiofilm activity of Cobetia marina filtrate upon Staphylococcus epidermidis catheter-related isolates BJM
Trentin,D.S; Gorziza,D. F; Abraham,W.R; Antunes,A.L.S; Lerner,C; Mothes,B; Termignoni,C; Macedo,A.J.
We report the antibiofilm activity by the sponge-associated bacterium Cobetia marina upon Staphylococcus epidermidis clinical isolates obtained from central venous catheters. Antibiofilm activity/antimicrobial susceptibility correlation might predict the action of the metabolite(s) upon Staphylococcus epidermidis in the clinic, making it a possible adjuvant in therapies against biofilm-associated infections.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Antibiofilm activity; Staphylococcus epidermidis; Cobetia marina; Darwinella sp.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400013
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Architecture and relevance of several strongly adhered biofilms over a polyester imide (PEI) surface BJM
Soncini-Júnior,Giancarlo; Franchetti,Sandra Mara Martins; Marconato,José Carlos.
A microscopical study was undertaken to analyze the eventual fungi adhesion over a polyester imide surface in enameled copper wires. Scanning electron microscopy allowed to observe in these adhered biofilms, a high amount of pigments, hyphae and its enzymatic arsenal probably acting in the surface of the polymer. Due to the highly aromatic nature of this polyester and the residual phenol derivates still present in the reticulated polymer, an antifungal activity could be expected, but no significant changes were noted in fungal growing and in the adhesion process. Additionally, the wires showed completely failure in all insulation properties. The studies aimed to understand and evaluate the great fungi potential that could be explored in biodeterioration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Biofilms; Hyphae; Pigments; Adhesion; Scanning electron microscopy; Polymeric surface; Polyester-imide; Enameled copper wires.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500036
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Bactericidia de hipoclorito de sodio sobre Staphylococcus cohnii productor de biofilm en una fabrica ABCL
Porcel de Fernandez,Norma Dolores; Urueña,Raquel; Gaudioso de Allori,Maria Cristina; de Castillo,Marta Elena Cecilia.
En equipamientos industriales la persistencia de las bacterias se debe al desarrollo de biofilms. El hipoclorito de sodio (NaClO) es usado rutinariamente como desinfectante. El objetivo del trabajo fue determinar el efecto inhibitorio del NaClO sobre S. cohnii productoras y no productoras de biofilms aisladas de una fabrica de pastas a partir de maquinarias de un establecimiento de Tucuman, Argentina. Se estudio produccion de biofilm, concentracion inhibitoria minima (CIM), concentracion bactericida minima (CBM) y curva de tiempo de muerte con NaClO frente a S. cohnii productora y no productora de biofilm. Los resultados obtenidos demostraron que de los ocho cocos grampositivos aislados, cuatro correspondieron a S. cohnii. Los valores CIM y CBM para S....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus cohnii; Biofilms; Hipoclorito de sodio; Actividad antimicrobiana.
Ano: 2013 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572013000400007
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Biofilm formation by Staphylococcus aureus and Salmonella spp. under mono and dual-species conditions and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite BJM
Iñiguez-Moreno,Maricarmen; Gutiérrez-Lomelí,Melesio; Guerrero-Medina,Pedro Javier; Avila-Novoa,María Guadalupe.
Abstract The aim of this study was evaluated the biofilm formation by Staphylococcus aureus 4E and Salmonella spp. under mono and dual-species biofilms, onto stainless steel 316 (SS) and polypropylene B (PP), and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite. The biofilms were developed by immersion of the surfaces in TSB by 10 d at 37 °C. The results showed that in monospecies biofilms the type of surface not affected the cellular density (p > 0.05). However, in dual-species biofilms on PP the adhesion of Salmonella spp. was favored, 7.61 ± 0.13 Log10 CFU/cm2, compared with monospecies biofilms onto the same surface, 5.91 ± 0.44 Log10 CFU/cm2 (p < 0.05). The mono and dual-species biofilms were subjected to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Salmonella spp.; Staphylococcus aureus; Disinfectants; Sensitivity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200310
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Biofilms: microbes and disease BJID
Aparna,Madhu Sharma; Yadav,Sarita.
Bacteria that attach to surface aggregate in a hydrated polymeric matrix of their own synthesis to form biofilms. These represent microbial societies with their own defense and communication system. Transitioning from acute to chronic infection is frequently associated with biofilm formation.Bacteria in biofilms are innately more resistant to antimicrobial agents. The presence of indwelling medical devices increases the risk for biofilm formation and subsequent infection. The current antibiotic therapies are of limited effectiveness in resolving biofilms infection.This review attempts to discuss the stages in biofilm formation, their pathogenic mechanisms, effect of antimicrobial agents, detection and eradication of the biofilms.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Staphylococci; Pseudomonas; Candida.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600016
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Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis. Repositório Alice
MELO, P. de C.; FERREIRA, L. M.; NADER FILHO, A.; ZAFALON, L. F.; VICENTE, H. I. G.; SOUZA, V. de.
Abstract: Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Mastite bovina; Phenotypic; Molecular analysis; Bovino; Doença animal; Mamite; Staphylococcus aureus; Biofilme; Estirpe; Bovine mastitis; Biofilms; Mammary gland diseases.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/960427
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Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis BJM
Melo,Poliana de Castro; Ferreira,Luciano Menezes; Nader Filho,Antônio; Zafalon,Luiz Francisco; Vicente,Hinig Isa Godoy; Souza,Viviane de.
Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Bovine mastitis; Phenotypic and molecular analysis; Staphylococcus aureus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100017
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Computational analysis suggests that virulence of Chromobacterium violaceum might be linked to biofilm formation and poly-NAG biosynthesis Genet. Mol. Biol.
Becker,Sidnei; Soares,Cíntia; Porto,Luismar Marques.
Groups of genes that produce exopolysaccharide with a N-acetyl-D-glucosamine monomer are in the genome of several pathogenic bacteria. Chromobacterium violaceum, an opportunistic pathogen, has the operon hmsHFR-CV2940, whose proteins can synthesize such polysaccharide. In this work, multiple alignments among proteins from bacteria that synthesize such polysaccharide were used to verify the existence of amino acids that might be critical for pathogen activity. Three-dimensional models were generated for spatial visualization of these amino acid residues. The analysis carried out showed that the protein HmsR preserves the amino acids D135, D228, Q264 and R267, considered critical for the formation of biofilms and, furthermore, that these amino acids are...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Exopolysaccharide; Chromobacterium violaceum pathogenicity; Comparative genomics.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300031
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Detección de microorganismos potencialmente patógenos en hogares de Mar del Plata ABCL
Gómez,Diana; Lavayén,Silvina; Nario,Flavia; Piquin,Andrea; Zotta,Claudio Marcelo.
La cocina y el baño son los lugares del hogar más susceptibles de contaminación microbiana. Estos microbios se depositan sobre las superficies formando biofilms y pueden constituirse en potenciales fuentes causales de enfermedades. El objetivo del trabajo fue detectar la presencia y la concentración de carga microbiana en los elementos y utensilios de los sitios más críticos de viviendas de la ciudad de Mar del Plata. Se recolectaron 478 muestras de elementos y utensilios de cocinas y baños provenientes de 36 hogares de familias de características socioeconómicas similares a los casos de Síndrome Urémico Hemolítico ocurridos en la misma ciudad. En cocinas el 52% superó los parámetros establecidos para el estudio detectándose >100 UFC/muestra de aerobios...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carga microbiana; Hogar; Biofilms; Síndrome urémico hemolítico.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572011000300005
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Different methods to quantify Listeria monocytogenesbiofilms cells showed different profile in their viability BJM
Winkelströter,Lizziane Kretli; Martinis,Elaine C.P. De.
Listeria monocytogenes is a foodborne pathogen able to adhere and to form biofilms in several materials commonly present in food processing plants. The aim of this study was to evaluate the resistance of Listeria monocytogenes attached to abiotic surface, after treatment with sanitizers, by culture method, microscopy and Quantitative Real Time Polymerase Chain Reaction (qPCR). Biofilms of L. monocytogenes were obtained in stainless steel coupons immersed in Brain Heart Infusion Broth, under agitation at 37 °C for 24 h. The methods selected for this study were based on plate count, microscopic count with the aid of viability dyes (CTC-DAPI), and qPCR. Results of culture method showed that peroxyacetic acid was efficient to kill sessile L. monocytogenes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; L. monocytogenes; QPCR; Peroxyacetic acid; Sodium hypochlorite.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100231
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Effect of alcohols on filamentation, growth, viability and biofilm development in Candida albicans BJM
Chauhan,Nitin M; Shinde,Ravikumar B; Karuppayil,S. Mohan.
In this study we report the potential of alcohols as morphogenetic regulators in Candida albicans. All the alcohols tested influenced various modes of growth like planktonic as well as biofilm forms. Viability was affected at high concentrations. Among the alcohols, the response of C. albicans to amyl alcohol (pentanol) was noteworthy. Amyl alcohol at a concentration 0.5% which was not inhibitory to growth and viability specifically inhibited morphogenetic switching from yeast to hyphal forms. It also inhibited normal biofilm development favoring yeast dominated biofilms. Based on this study we hypothesize that alcohols produced under anaerobic conditions may not favor biofilm development and support dissemination of yeast cells. Since anaerobic conditions...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alcohols; Signalling; Morphogenesis; Biofilms; Yeast metabolism; Candida albicans.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400041
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Effect of modified clays on the structure and functional properties of biofilms produced with zein Ciênc. Tecnol. Aliment.
Tavares,Luciana Lopes; Almeida,Crislene Barbosa de; Caruso,Ícaro Putinhon; Cornélio,Marinônio Lopes; Lopes Filho,José Francisco.
The purpose of this study was to evaluate changes in the structure and some functional properties of biofilms added with modified clays (Cloisite® 15A and Cloisite® 30B) prepared by the casting method. The analysis of the microstructure of the films, scanning electron microscopy (SEM), Optical microscopy (MO), and Infrared Spectroscopy (FTIR) indicated that the addition of clay in the films resulted in the formation of a heterogeneous microstructure, microcomposite or tactoid. Due to the formation of a microcomposite structure, functional properties of the films added with both clays such as opacity, solubility, and permeability to water vapor (PVA), were not better than those of the control film. Thus, it was concluded that although it is possible to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Zein; Clays; Microcomposite; Tactoids.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000200017
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Effects of Myrcia ovata Cambess. essential oil on planktonic growth of gastrointestinal microorganisms and biofilm formation of Enterococcus faecalis BJM
Cândido,Cinthya S.; Portella,Cadmo Silton A.; Laranjeira,Bruno J.; Silva,Sérgio S. da; Arriaga,Angela M.C.; Santiago,Gilvandete M. P.; Gomes,Geovany A.; Almeida,Paulo César; Carvalho,Cibele B. M..
The essential oil from the leaves of Myrcia ovata Cambess., commonly used in Brazil for the treatment of gastric illnesses, was screened for antimicrobial activity and action in the formation of microbial biofilms by Enterococcus faecalis. The oil was obtained by hydrodistillation using a clevenger-type system. Its chemical composition was analyzed using GC and GC-MS. Both MIC and MBC of the essential oil were determined by broth microdilution techniques and agar dilution method. The essential oil showed antimicrobial activity against E. faecalis, Escherichia coli, Pseudomonas aeruginosa, Salmonella choleraesuis, Staphylococcus aureus, Streptococcus pneumoniae and Candida parapsilosis. The results showed that the essential oil of M. ovata Cambess. was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oil; Myrcia ovata Cambess.; Antimicrobial activity; Biofilms.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300012
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Evaluation of different detection methods of biofilm formation in the clinical isolates BJID
Hassan,Afreenish; Usman,Javaid; Kaleem,Fatima; Omair,Maria; Khalid,Ali; Iqbal,Muhammad.
BACKGROUND: Microorganisms growing in a biofilm are associated with chronic and recurrent human infections and are highly resistant to antimicrobial agents. There are various methods to detect biofilm production like Tissue Culture Plate (TCP), Tube method (TM), Congo Red Agar method (CRA), bioluminescent assay, piezoelectric sensors, and fluorescent microscopic examination. OBJECTIVE: This study was conducted to compare three methods for the detection of biofilms. METHOD: The study was carried out at the Department of Microbiology, Army Medical College, National University of Sciences and Technology, Pakistan, from January 2010 to June 2010. A total of 110 clinical isolates were subjected to biofilm detection methods. Isolates were identified by standard...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Bacteria; Anti-bacterial agents.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400002
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Expression of virulence genes by Listeria monocytogenes J0161 in natural environment BJM
Tirumalai,Prem Saran; Prakash,Soam.
Majority of studies concerning the gene expression of Listeria monocytogenes have been done on pure culture states. Our objective was to study L.monocytogenes in a co-cultured state and to understand if microbes in their natural state of existence are different in their expression than that of the purely cultured lab grown forms. For a long period discussions have been on the expression of prfA, (which is a virulence gene regulator) in a mammalian host and its role in causing the switch from a saprophytic to pathogenic form of L.monocytogenes. We, in this paper for the first time report the expression of prfA and other virulence genes by L.monocytogenes under different extracellular conditions, and also as a pure culture biofilms, that is different from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; PrfA; Virulence; Microarray; Biofilms; Co-culture; Gene Expression.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200050
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Extended Spectrum Beta-Lactamase Production and Biofilm Formation in Salmonella Serovars Resistant to Antimicrobial Agents Rev. Bras. Ciênc. Avic.
Mandelli,JZA; Ehrhardt,A; Manto,L; Borges,KA; Furian,TQ; Weber,B; Rodrigues,LB; Santos,LR.
ABSTRACT Antimicrobial resistance is a serious public health problem and Salmonella spp. is highly resistant to antimicrobial agents. Biofilms are important in the food industry due to their formation on products, utensils, and surfaces and the difficulty in their removal. The objective of this study was to assess extended spectrum beta-lactamase (ESBL) production, antimicrobial resistance, and biofilm production of Salmonella isolated from poultry slaughterhouses. Antimicrobial susceptibility was assessed by the disk diffusion assay and ESBL by double diffusion disk assay using the beta-lactamase inhibitor (amoxicillin+clavulanate). The antimicrobials tested were: ampicillin, amoxicillin+clavulanate, aztreonam, ceftazidime, cefotaxime, chloramphenicol,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Drug resistance; ESBL; Salmonella.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200348
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Factors Involved in the Persistence of a Shiga Toxin-Producing Escherichia coil O157:H7 Strain in Bovine Feces and Gastro-Intestinal Content ArchiMer
Segura, Audrey; Auffret, Pauline; Bibbal, Delphine; Bertoni, Marine; Durand, Alexandra; Jubelin, Gregory; Kerouredan, Monique; Brugere, Hubert; Bertin, Yolande; Forano, Evelyne.
Healthy cattle are the primary reservoir for O157:H7 Shiga toxin-producing E coil responsible for human food-borne infections. Because farm environment acts as a source of cattle contamination, it is important to better understand the factors controlling the persistence of E. coil O157:H7 outside the bovine gut. The E. coil O157:H7 strain MC2, identified as a persistent strain in French farms, possessed the characteristics required to cause human infections and genetic markers associated with clinical O157:H7 isolates. Therefore, the capacity of E. coil MC2 to survive during its transit through the bovine gastro-intestinal tract (GIT) and to respond to stresses potentially encountered in extra intestinal environments was analyzed. E. coil MC2 survived in...
Tipo: Text Palavras-chave: Escherichia coli; STEC; EHEC; O157:H7; Biofilms; Stress response; Persistence.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00434/54588/55972.pdf
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Formación de biopelículas y resistencia a desinfectantes en cepas de salmonella aisladas de nopal verdura y muestras de agua y suelo de uso agrícola. Colegio de Postgraduados
Santos Villamil, Alfricia Adriana de los.
La presencia de Salmonella en nopal verdura fresco (Opuntia ficus-indica L.) MILL se ha documentado recientemente (Hernández et al., 2009). La contaminación de alimentos es un problema de salud pública que afecta a las personas en todo el mundo. Una característica importante de Salmonella es su capacidad para formar biopelículas, las cuales son comunidades de células microbianas embebidas en una matriz de exopolímeros que ellas mismas producen en respuesta a factores como la temperatura, la humedad relativa y la composición del medio en que se encuentran (Annous et al., 2005; Stepanovic et al., 2000). Esta capacidad le permite a Salmonella adherirse de manera irreversible y de colonizar superficies bióticas y abióticas (Annous et al., 2005, Stepanovic et...
Palavras-chave: Biopelículas; Desinfectantes; Nopal; Salmonella; Biofilms; Desinfectants; Maestría; Fitopatología.
Ano: 2010 URL: http://hdl.handle.net/10521/220
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Formación de biopelículas y resistencia a desinfectantes en cepas de salmonella aisladas de nopal verdura y muestras de agua y suelo de uso agrícola. Colegio de Postgraduados
Santos Villamil, Alfricia Adriana de los.
La presencia de Salmonella en nopal verdura fresco (Opuntia ficus-indica L.) MILL se ha documentado recientemente (Hernández et al., 2009). La contaminación de alimentos es un problema de salud pública que afecta a las personas en todo el mundo. Una característica importante de Salmonella es su capacidad para formar biopelículas, las cuales son comunidades de células microbianas embebidas en una matriz de exopolímeros que ellas mismas producen en respuesta a factores como la temperatura, la humedad relativa y la composición del medio en que se encuentran (Annous et al., 2005; Stepanovic et al., 2000). Esta capacidad le permite a Salmonella adherirse de manera irreversible y de colonizar superficies bióticas y abióticas (Annous et al., 2005, Stepanovic et...
Palavras-chave: Biopelículas; Desinfectantes; Nopal; Salmonella; Biofilms; Desinfectants; Maestría; Fitopatología.
Ano: 2010 URL: http://hdl.handle.net/10521/220
Registros recuperados: 33
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