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| Registros recuperados: 166 | |
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| Vaz, Madalena; Jorge, Lurdes. |
| Com o objectivo de identificar genes, avaliaram-se nove EST (“Expressed Sequence Tag”) de uma biblioteca de cDNA de Trichoderma harzianum. Numa primeira etapa, as ESTs foram sequenciadas, sendo as sequências analisadas com programas bioinformáticos de análise de similaridade em bancos de dados, utilizando ferramentas como Blast P e Fasta X. Identificaram-se duas ESTs que se enquadravam nos objectivos, sendo uma delas a EST-1279, usada para a completa elucidação do gene lip2 de Trichoderma harzianum, cuja sequência pode ser acedida na base de dados com os códigos de acesso AM774154. O gene lip2 de Trichoderma harzianum sequenciado apresenta 1992 pb, sendo a sua ORF (“open reading frame”) constituída por 1215 nucleótidos. Estão sequenciados 550 pb da região... |
| Tipo: ConferenceObject |
Palavras-chave: Bioinformática; Identificação de genes. |
| Ano: 2011 |
URL: http://hdl.handle.net/10198/5717 |
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| HERAI, R. H.; YAMAGISHI, M. E. B.. |
| Trans-splicing is an unusual form of RNA processing mechanism where distinct pre-mRNA sequences contribute to the formation of a single mRNA. It is common in nematodes and kinetoplastids, but it is rare in superior organisms. Nevertheless, some recent studies show that trans-splicing, although rare, may be more widespread than believed (Gingeras). Consequently, its identification in large databanks is very important for posteriori experimental confirmation. Recently, we have developed a Bioinformatic methodology that successfully found 16 inter-chromosomal trans-splicing candidate sequences in a large human full length cDNA (FlcDNA) databank (Herai & Yamagishi, in press). Unfortunately, our methodology was supposed to be applied on organisms with high... |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Bioinformática; Algoritmo; Algorithms; Bioinformatics; RNA splicing. |
| Ano: 2010 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/868482 |
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| PESSOA FILHO, M. A. C. de P.; SOUZA SOBRINHO, F. de; FRAGOSO, R. da R.; SILVA JUNIOR, O. B. da; FERREIRA, M. E.. |
| ABSTRACT: Ruzigrass (Urochloa ruziziensis) is a diploid, tropical forage grass native to Africa, widely planted in Brazil, and known for its high nutritional quality. It is closely related to other important forage species of Urochloa, playing a crucial role in the breeding program of brachiaria grasses, which is mostly focused on inter-specific hybrids. Previous studies from our group based on shallow Illumina sequencing resulted in the development of the first molecular markers for the species, as well as in assessments of germplasm diversity and structure. Assembly and analysis of complete plastid genomes for four Urochloa species allowed the characterization of their phylogenetic divergence. Here, we strengthen the set of genomic tools for tropical... |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Montagem de sequência de DNA; Bioinformática; De novo Assembly; Brachiaria Ruziziensis; Pastagem; Genoma. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099953 |
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| NASCIMENTO, L. C. do; COSTA, G. G. L.; BINNECK, E.; PEREIRA, G. A. G.; CARAZZOLLE, M. F.. |
| The Genosoja consortium is an initiative to integrate different omics research approaches carried out in Brazil. Basically, the aim of the project is to improve the plant by identifying genes involved in responses against stresses that affect domestic production, like drought stress and Asian Rust fungal disease. To do so, the project generated several types of sequence data using different methodologies, most of them sequenced by next generation sequencers. The initial stage of the project is highly dependent on bioinformatics analysis, providing suitable tools and integrated databases. In this work, we describe the main features of the Genosoja web database, including the pipelines to analyze some kinds of data (ESTs, SuperSAGE, microRNAs, subtractive... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Bioinformática; Expressão genética; Soja; Gene; Soybeans; Genes; Bioinformatics; Gene expression. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/926586 |
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| BINNECK, E.; NASCIMENTO, L. S. do. |
| The availability of the soybean genome sequence and the rapid evolution of DNA sequencing technologies in recent years bring us exciting new promises for scientific discovery and its conversion into required technological breakthroughs in the production and use of soybean. In addition to be a major protein source in animal feeds, soybean is ranked number one among the chief oil crops in the world and is becoming a major crop for biodiesel production. As a leguminous crop, soybean fixes nitrogen in association with Bradyrhizobium, which significantly reduces the needs for ammonium-based fertilizer even in other crops. In this presentation, it will be described using a web-based platform, Soybean Gene Express, for analyzing and storing RNA-Seq data from... |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Bioinformática; Soja; Gene; Genoma; Soybeans; Gene expression. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/943038 |
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| SOUZA, M. M.; MOKRY, F. C.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; SOMAVILLA, A.; CESAR, A. S. M.; MORÉ, D.; MOURÃO, G.; DINIZ, W. J. S.; MUDADU, M. A.; NICIURA, S. C. M.; COUTINHO, L. L.; ZERLOTINI, A.; REGITANO, L. C.. |
| Imprinted genes have been target of many studies, mainly in human and mouse, and lately in bovines due to the interest of understanding the epigenetic mechanisms underlying important meat quality phenotypes and the possibility of applying it in animal breeding programs in the future. Genomic DNA from 146 steers was genotyped using the Illumina BovineHD BeadChip in order to identify heterozygous individuals with known allele origin. |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Bioinformática; Expressão gênica; Genoma; Bioinformatics; Genome; Gene expression. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035029 |
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| SOUZA, M. M.; MOKRY, F. C.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; SOMAVILLA, A.; CESAR, A. S. M.; MORÉ, D.; MOURÃO, G.; DINIZ, W. J. S.; MUDADU, M. A.; NICIURA, S. C. M.; COUTINHO, L. L.; ZERLOTINI NETO, A.; REGITANO, L. C.. |
| Imprinted genes have been target of many studies, mainly in human and mouse, and lately in bovines due to the interest of understanding the epigenetic mechanisms underlying important meat quality phenotypes and the possibility of applying it in animal breeding programs in the future. Genomic DNA from 146 steers was genotyped using the Illumina BovineHD BeadChip in order to identify heterozygous individuals with known allele origin. |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Bioinformática; Expressão gênica; Genoma; Bioinformatics; Gene expression; Genome. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035220 |
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| WANDERLEY-NOGUEIRA, A. C.; BELARMINO, L. C.; SOARES-CAVALCANTI, N. da M.; BEZERRA-NETO, J. P.; KIDO, E. A.; PANDOLFI, V.; ABDELNOOR, R. V.; BINNECK, E.; CARAZZOLE, M. F.; BENKO-ISEPPON, A. M.. |
| Plants have the ability to recognize and respond to a multitude of pathogens, resulting in a massive reprogramming of the plant to activate defense responses including Resistance (R) and Pathogenesis-Related (PR) genes. Abiotic stresses can also activate PR genes and enhance pathogen resistance, representing valuable genes for breeding purposes. The present work offers an overview of soybean R and PR genes present in the GENOSOJA (Brazilian Soybean Genome Consortium) platform, regarding their structure, abundance, evolution and role in the plantpathogen metabolic pathway, as compared with Medicago and Arabidopsis. Searches revealed 3,065 R candidates (756 in Soybean, 1,142 in Medicago and 1,167 in Arabidopsis), and PR candidates matching to 1,261 sequences... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Bioinformática; Biotic stress; Pathogen response; Soja; Resposta da planta; Doença de planta; Gene; Genoma; Soybeans; Bioinformatics; Plant diseases and disorders; Genome; Genes. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/926612 |
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| Deusdado, Sérgio. |
| A informação dos códigos genéticos sequenciados é na actualidade, provavelmente, a fonte mais inspiradora para o estudo e avanço das teorias da informação e da codificação. Algoritmos eficientes para a sua compressão antevêm-se essenciais para a optimização do armazenamento e comunicação da informação genómica. A compressão de informação genómica é um caso particular da compressão de informação. A entropia das sequências de ADN é elevada, contudo variável. Ao nível intra-genómico é maior nas regiões codificantes e menor nas regiões não codificantes. Ao nível inter-genómico é maior nos seres procarióticos e menor nos eucarióticos. Na base da redução da entropia estão as regularidades que perfazem as regiões repetitivas do ADN. As regiões repetitivas... |
| Tipo: DoctoralThesis |
Palavras-chave: Compressão; Bioinformática. |
| Ano: 2008 |
URL: http://hdl.handle.net/10198/968 |
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| Registros recuperados: 166 | |
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