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Actual and potential heterotrophicneutrogen fixation and detrification rates of oryza glumaepatula Steud in an amazonian lake BJB
ENRICH-PRAST,A.; ESTEVES,F. A.; BIESBOER,D. D..
Estimates of the rates of heterotrophic nitrogen fixation (HNF) and denitrification by acetylene reduction and acetylene inhibition, respectively, were determined for the roots and culms of wild rice, Oryza glumaepatula, in Lake Batata, a lake severely impacted by the dumping of bauxite tailings into the lake basin. Two stations were established in different parts of the lake: one in a natural area and other in an impacted area of the lake. Plant assays were performed during the principle hydroperiods of dry (December) and flood (July) to determine both: a) Actual rates of HNF and denitrification, and b) potential rates of HNF after amending with glucose potential rates of denitrification after amending with glucose, glucose + nitrate, or nitrate alone....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological nitrogen fixation; Amazonian lake; Aquatic macrophyte.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71081999000300013
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Agronomic efficiency of Rhizobium strains from the Amazon region in common bean Acta Amazonica
NOGUEIRA,Cláudia de Oliveira Gonçalves; OLIVEIRA,Dâmiany Pádua; FERREIRA,Paulo Ademar Avelar; PEREIRA,João Paulo de Andrade Rezende; VALE,Helson Mário Martins do; ANDRADE,Messias José Bastos de; MOREIRA,Fatima Maria de Souza.
ABSTRACT To maximize the contribution of biological nitrogen fixation in common bean, it is necessary to use bacterial strains that are more adapted, competitive, and efficient in the symbiotic process. In this regard, the aim of this study was to evaluate the agronomic efficiency (AE) of three bacterial strains isolated from acid soils with high Al content from the Amazon region in an Argissolo Vermelho Distrófico típico soil (Typic Rhodustults - USDA Classification) from the municipality of Formiga, MG, Brazil. We compared their AE to that of the reference strain CIAT 899T and of two controls without inoculation (one without and another with 80 kg ha-1 of N-urea). The results indicated that inoculation with the strains UFLA 02-100 and UFLA 02-127...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Phaseolus vulgaris L.; Rhizobia; Biological nitrogen fixation; Seed inoculation.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000300273
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Alfalfa yield and quality as function of nitrogen fertilization and symbiosis with Sinorhizobium meliloti Scientia Agricola
Oliveira,Wladecir Salles de; Oliveira,Patrícia Perondi Anchão; Corsi,Moacyr; Duarte,Fábio Rodrigo Sanches; Tsai,Siu Mui.
The efficiency of the symbiotic process between alfalfa (Medicago sativa L.) and Sinorhizobium meliloti can be drastically affected by soil nitrogen. This research evaluates how mineral fertilization affects the contribution of symbiosis to the yield and quality of alfalfa in two experiments under controlled conditions, and a third one in the field. Under controlled conditions, positive effect of nitrogen fertilizer was observed, whereas, in the field, negative effect of the mineral nitrogen (450 kg ha-1 year-1) on the symbiotic process was observed, with reduction of nodulation and nitrogenase activity. However, there was no effect on yield, total nitrogen concentration, crude protein, non-protein nitrogen and digestibility in relation to the treatment...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicago sativa L.; Biological nitrogen fixation.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400013
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Bacterial strains from floodplain soils perform different plant-growth promoting processes and enhance cowpea growth Scientia Agricola
Costa,Elaine Martins da; Carvalho,Fernanda de; Nóbrega,Rafaela Simão Abrahão; Silva,Jacqueline Savana; Moreira,Fatima Maria de Souza.
ABSTRACT Certain nodulating nitrogen-fixing bacteria in legumes and other nodule endophytes perform different plant-growth promoting processes. The objective of this study was to evaluate 26 bacterial strains isolated from cowpea nodules grown in floodplain soils in the Brazilian savannas, regarding performance of plant-growth promoting processes and ability to enhance cowpea growth. We also identified these strains by 16S rRNA sequencing. The following processes were evaluated: free-living biological nitrogen fixation (BNF), solubilization of calcium, aluminum and iron phosphates and production of indole-3-acetic acid (IAA). The abilities to nodulate and promote cowpea growth were evaluated in Leonard jars. Partial sequencing of the 16S rRNA gene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paenibacillus; Biotechnological potential; Biological nitrogen fixation; Phosphate solubilization; Indole-3-acetic acid.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000400301
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Balanço de N em rotação de culturas sob plantio direto em Dourados, MS. Infoteca-e
ALVES, B. J. R.; ZOTARELLI, L.; ARAÚJO, E. S.; FERNANDES, F. M.; HECKLER, J. C.; MEDEIROS, A. F. A.; BODDEY, R. M.; URQUIAGA, S..
Matéria seca e N total. Quantificação da FBN eficiência do uso de N fertilizante. Balanço de N para as culturas. Resultados e discussão. Quantificação da FBN para a cultura da soja. Eficiência da fertilização. Balança de N para as culturas. O objetivo do presente estudo foi avaliar o impacto da fixação biológica de nitrogênio (FBN) na cultura da soja e a eficiência do uso de fertilizantes nitrogenados pelas culturas do milho e algodão sobre o balanço de N dos solo. O experimento foi realizando em Latossolo Vermelho distroférrico, na área experimental da Embrapa agropecuária oeste, em dourados, MS. Todas as culturas foram conduzidas em uma área onde se iniciou o plantio direto. No primeiro ano , as culturas do milho e algodão receberam 115kg N há-1 ,...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Balanço de nitrogênio; Fixação biológica de nitrogênio; FBN; Zero tillage; Direct sowing; Rotational cropping; Biological nitrogen fixation; BNF; Soybean; Maize.; Algodão; Milho; Plantio Direto; Rotação de Cultura; Soja.; Cotton; Nitrogen balance..
Ano: 2005 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/626863
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Biodiversity of rhizobia associated with cowpea cultivars in soils of the lower half of the São Francisco River Valley Rev. Bras. Ciênc. Solo
Leite,Jakson; Seido,Sirando Lima; Passos,Samuel Ribeiro; Xavier,Gustavo Ribeiro; Rumjanek,Norma Gouvêa; Martins,Lindete Míria Vieira.
The biodiversity of rhizobium in soils of the São Francisco Valley is unknown and can be studied using cowpea as trap plants. The objective of this study was to verify the diversity of diazotrophic bacteria that nodulate cowpea in soils of the lower half of the São Francisco River Valley by morphological and genotypic characterization. Seven soil samples (A1, A2, A3, A4, C1, C2 and MC) were collected to capture bacteria associated to five cowpea cultivars (IPA 206, BRS Pujante, BRS Marataoã, Canapu Roxo, and Sempre Verde), in a 5x7 factorial design with three replications. Thirty days after plant emergence, the nodules were collected and the bacteria isolated and analyzed in relation to their growth characteristics in YMA medium. The 581 isolates were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ARDRA; Biological nitrogen fixation; Vigna unguiculata.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000500015
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Biological fixation of nitrogen by native isolates of Rhizobium sp. symbionts of Leucaena leucocephala (Lam.) De Wit Acta Agron. (Palmira)
Cubillos-Hinojosa,Juan Guillermo; Milian-Mindiola,Pablo Ernesto; Hernández-Mulford,Jorge Luis; Peralta-Castilla,Arnaldo.
Abstract Forage legumes such as Leucaena leucocephada, besides being a rich source of protein for animal feed, its inclusion in silvopastoral systems provides fundamental components to improve soil properties. These plants have the ability to establish symbiotic association with the genus Rhizobium sp. and fix biologically atmospheric nitrogen favoring growth and development, being reflected in production increases. The objective of this research was to evaluate the capacity of native isolates of Rhizobium sp. and a commercial strain in biological nitrogen fixation (BNF) in L. leucocephala plants. Two separate bioassays to evaluate the efficiency of the FBN of the native isolates and the commercial strain under greenhouse conditions, followed by a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leucaena leucocephala; Biological nitrogen fixation; Forage legumes; Rhizobium sp.
Ano: 2019 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122019000200075
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Bradyrhizobium spp. Strains in Symbiosis with Pigeon Pea cv. Fava-Larga under Greenhouse and Field Conditions Rev. Bras. Ciênc. Solo
Rufini,Márcia; Oliveira,Dâmiany Pádua; Trochmann,André; Soares,Bruno Lima; Andrade,Messias José Bastos de; Moreira,Fatima Maria de Souza.
ABSTRACT: Optimization of symbiosis between nitrogen-fixing bacteria and legumes has been extensively studied, seeking agricultural sustainability. To evaluate the symbiotic efficiency of nitrogen-fixing bacterial strains belonging to the Bradyrhizobium genus with pigeon pea (Cajanus cajan (L.) Millsp.) cv. Fava-Larga, experiments were conducted in Leonard jars (axenic conditions), pots with soil, and in the field. Ten strains were tested in Leonard jars, and three strains, in addition to BR 29, were selected according to their ability to promote the growth of pigeon pea, for further tests in pots with different soil types (Inceptsol and Oxisol) and in the field (Oxisol). Treatments were compared with strains BR 2003 and BR 2801 (approved as inoculants for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cajanus cajan; Inoculant; Biological nitrogen fixation; Selection.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832016000100432
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Characterization of bacterial endophytes from the roots of native and cultivated Brazil nut trees (Bertholletia excelsa) Acta Amazonica
CHALITA,Patrícia Bombonati; FARIAS,Eliane do Nascimento Cunha; COSTA,Ismaele Breckenfeld da; SOUSA,Brenda Ferreira; SANTOS,Marco Antônio Oliveira dos; ALBUQUERQUE,Teresinha Costa Silveira de; VITAL,Marcos José Salgado; SILVA,Krisle da.
ABSTRACT Brazil nut is a very important nontimber forest product in the Amazon region. Propagation of this tree still represents a challenge due to slow and uneven seed germination. In this context, plant growth-promoting bacteria can facilitate the process of propagation. The aims of this study were to isolate and characterize endophytic bacteria from the roots of Brazil nut trees in native terra firme forest and cultivation areas in northern Brazil, and to identify mechanisms by which bacteria act in plant growth promotion. Overall, 90 bacterial isolates were obtained from the roots of Brazil nut trees in monoculture, agroforestry and native forest areas by using different semisolid media. The isolates were characterized by sequencing the 16S rRNA gene....
Tipo: Info:eu-repo/semantics/article Palavras-chave: 16S rRNA; Phosphate solubilization; Indole compounds; Biological nitrogen fixation; Plant growth-promotion bacteria; Amazon.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000400257
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Common bean elite lines cultivated under nitrogen fertilization and inoculation with Rhizobium tropici Ciência Rural
Pereira,Helton Santos; Melo,Leonardo Cunha; Faria,Luís Cláudio de; Ferreira,Enderson Petrônio de Brito; Mercante,Fábio Martins; Wendland,Adriane; Souza,Thiago Lívio Pessoa Oliveira de.
ABSTRACT: More efficient strains of Rhizobium have been selected for use in common bean. However, little effort was made with lines selection. The main goals of this research were to verify the presence of interaction involving common bean elite lines utilizing Nitrogen fertilization and Rhizobium inoculation for grain yield and to identify lines with superior yields utilizing biological nitrogen fixation. Eight field trials were conducted at four location-years in Brazilian savanna, using randomized complete blocks design with three replications. Each trial was composed of 17 carioca elite lines. Every two tests in each location were planted side by side, one with mineral nitrogen fertilization (90kg) and the other one with inoculation with Rhizobium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Biological nitrogen fixation; Grain yield; Genotype by environment interaction.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001202168
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Comparison of 15N isotope methods to determine the recovery efficiency of nitrogen from green manure PAB
Araújo,Ednaldo da Silva; Silva,Bárbara Fernanda da; Chieza,Emerson Dalla; Urquiaga,Segundo; Guerra,José Guilherme Marinho; Costa,Janaína Ribeiro; Espíndola,Jose Antonio Azevedo.
Abstract: The objective of this work was to compare three methods of 15N isotope application to the soil in order to determine the recovery efficiency of nitrogen derived from green manure by corn (Zea mays). The used experimental design was a randomized complete block with six replicates. The treatments consisted of three 15N isotope methods: indirect method I, isotope dilution with 15N-labelled synthetic fertilizer applied through a small hole next to each corn plant; indirect method II, isotope dilution with 15N-labelled synthetic fertilizer spread in the furrow next to the corn planting row; and direct method, application of a legume straw as green manure, treated with 2.23% excess 15N. The green manure used was jack bean (Canavalia ensiformis)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Biological nitrogen fixation; Legume.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000103201
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Components of corn crop yield under inoculation with Azospirillum brasilense using integrated crop-livestock system Agronomy
Brum, Marcos da Silva; Cunha, Vinícius Dos Santos; Stecca, Jéssica Deolinda Leivas; Grando, Luiz Fernando Teleken; Martin, Thomas Newton.
The objective of this study was to evaluate the agronomic characteristics of corn seed inoculated with Azospirillum brasilense, grown on black oat and ryegrass straw, and managed under different grazing strategies and doses of nitrogen. The experiment was conducted in Santa Maria, Rio Grande do Sul State, Brazil, during two agricultural seasons (2012/2013 and 2013/2014) in a randomized, complete block design with three replications. In the winter period, black oat and ryegrass straw were managed at different grazing heights by sheep (0.30, 0.20, 0.10 m, conventional grazing, and no grazing) with three doses of nitrogen (0, 50, and 100 kg ha-1, with or without inoculation by A. brasilense). We used the hybrid Pioneer (P1630H®) in 2012 and the hybrid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.00.00-9 Zea mays; Grazing heights; Biological nitrogen fixation; Nitrogen; Integrated systems. Fitotecnia.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/30664
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Crop rotation biomass and arbuscular mycorrhizal fungi effects on sugarcane yield Scientia Agricola
Ambrosano,Edmilson José; Azcón,Rozario; Cantarella,Heitor; Ambrosano,Gláucia Maria Bovi; Schammass,Eliana Aparecida; Muraoka,Takashi; Trivelin,Paulo César Ocheuze; Rossi,Fabrício; Guirado,Nivaldo; Ungaro,Maria Regina Gonçalves; Teramoto,Juliana Rolim Salomé.
A cana-de-açúcar (Saccharum spp.) vem sendo cultivada no Brasil para produção de açúcar e agroenergia. Em seu sistema de produção, após um ciclo de 4 a 8 anos, é possível a rotação com plantas de cobertura, antes do seu replantio, para melhoria do solo e geração de renda. Estudou-se a caracterização e produtividade de biomassa de leguminosas (como adubos-verdes) e girassol (Helianthus annuus L.), a ocorrência natural de micorrizas, o teor de açúcar e a produtividade em colmos da cana-de-açúcar IAC 87-3396 e a viabilidade econômica desse sistema com cultivo após as opções de rotação, com quantificação da produtividade durante três cortes consecutivos. O amendoim (Arachis hypogaea L.) cv. IAC-Caiapó, girassol cv. IAC-Uruguai e mucuna-preta (Mucuna aterrimum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Green manure; Legumes; Biological nitrogen fixation; Sugarcane stalk yield.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000600011
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Diazotrophic bacteria and substrates in the growth and nitrogen accumulation of sugarcane seedlings Scientia Agricola
Matoso,Ester Schiavon; Reis,Veronica Massena; Giacomini,Sandro José; Silva,Mariana Teixeira da; Avancini,Anita Ribas; Silva,Sérgio Delmar dos Anjos e.
ABSTRACT Little is known about the interaction between the inoculation of diazotrophic bacteria, the variety, and the substrate used in inoculated sugarcane seedling production. Bearing this in mind, the aim of this study was to evaluate the effects of diazotrophic bacteria inoculation, four sugarcane varieties and four different substrates on the growth and nitrogen accumulation of sugarcane seedlings. Parameters related to sprouting, height, and root growth, as well as nitrogen accumulation, were evaluated. The results indicate that inoculating sugarcane seedlings belonging to the following varieties: RB867515, RB92579, RB966928 and RB975932 with bacteria may increase sprouting speed index, height, root length, fresh and dry matter weight, as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharum sp.; Carbonized rice husk; Organic compost; Biological nitrogen fixation.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000101403
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Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region Acta Amazonica
SILVA,Krisle da; PERIN,Liamara; GOMES,Maria de Lourdes; BARAÚNA,Alexandre Cardoso; PEREIRA,Gilmara Maria Duarte; MOSQUEIRA,Cátia Aparecida; COSTA,Ismaele Breckenfeld da; O'HARA,Graham; ZILLI,Jerri Édson.
ABSTRACT Maize plants can establish beneficial associations with plant growth-promoting bacteria. However, few studies have been conducted on the characterization and inoculation of these bacteria in the Amazon region. This study aimed to characterize endophytic bacteria isolated from maize in the Amazon region and to assess their capacity to promote plant growth. Fifty-five bacterial isolates were obtained from maize grown in two types of ecosystems, i.e., a cerrado (savanna) and a forest area. The isolates were characterized by the presence of the nifH gene, their ability to synthesize indole-3-acetic acid (IAA) and solubilize calcium phosphate (CaHPO4), and 16S rRNA partial gene sequencing. Twenty-four bacteria contained the nifH gene, of which seven...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological nitrogen fixation; Zea mays L.; 16S rRNA.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672016000200111
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Diversity and nitrogen fixation efficiency of rhizobia isolated from nodules of Centrolobium paraense PAB
Baraúna,Alexandre Cardoso; Silva,Krisle da; Pereira,Gilmara Maria Duarte; Kaminski,Paulo Emílio; Perin,Liamara; Zilli,Jerri Edson.
The objective of this work was to isolate and characterize rhizobia from nodules of Centrolobium paraense and to evaluate their symbiotic efficiency. Soil samples collected from four sites of the Roraima Cerrado, Brazil, were used to cultivate C. paraense in order to obtain nodules. Isolates (178) were obtained from 334 nodules after cultivation on medium 79. Twenty-five isolates belonging to six morphological groups were authenticated using Vigna unguiculata and they were characterized by 16S rRNA. Isolates identified as Bradyrhizobium were further characterized using rpoB gene sequencing. A greenhouse experiment was carried out with C. paraense to test the 18 authenticated isolates. Approximately 90% of the isolates grew slowly in medium 79. The 16S rRNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bradyrhizobium; Rhizobium; Bacteria diversity; Biological nitrogen fixation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2014000400296
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Draft genome sequence of Bradyrhizobium manausense strain BR 3351T, an effective symbiont isolated from Amazon rainforest BJM
Simões-Araújo,Jean Luiz; Rumjanek,Norma Gouvêa; Xavier,Gustavo Ribeiro; Zilli,Jerri Édson.
ABSTRACT The strain BR 3351T (Bradyrhizobium manausense) was obtained from nodules of cowpea (Vigna unguiculata L. Walp) growing in soil collected from Amazon rainforest. Furthermore, it was observed that the strain has high capacity to fix nitrogen symbiotically in symbioses with cowpea. We report here the draft genome sequence of strain BR 3351T. The information presented will be important for comparative analysis of nodulation and nitrogen fixation for diazotrophic bacteria. A draft genome with 9,145,311 bp and 62.9% of GC content was assembled in 127 scaffolds using 100 bp pair-end Illumina MiSeq system. The RAST annotation identified 8603 coding sequences, 51 RNAs genes, classified in 504 subsystems.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazon forest; Biological nitrogen fixation; Next generation sequencing; Nodulation; Cowpea.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400610
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Draft genome sequence of Bradyrhizobium sp. strain BR 3262, an effective microsymbiont recommended for cowpea inoculation in Brazil BJM
Simões-Araújo,Jean Luiz; Leite,Jakson; Marie Rouws,Luc Felicianus; Passos,Samuel Ribeiro; Xavier,Gustavo Ribeiro; Rumjanek,Norma Gouvêa; Zilli,Jerri Édson.
Abstract The strain BR 3262 was isolated from nodule of cowpea (Vigna unguiculata L. Walp) growing in soil of the Atlantic Forest area in Brazil and it is reported as an efficient nitrogen fixing bacterium associated to cowpea. Firstly, this strain was assigned as Bradyrhizobium elkanii, however, recently a more detailed genetic and molecular characterization has indicated it could be a Bradyrhizobium pachyrhizi species. We report here the draft genome sequence of B. pachyrhizi strain BR 3262, an elite bacterium used as inoculant for cowpea. The whole genome with 116 scaffolds, 8,965,178 bp and 63.8% of C+G content for BR 3262 was obtained using Illumina MiSeq sequencing technology. Annotation was added by the RAST prokaryotic genome annotation service and...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Atlantic forest area; Biological nitrogen fixation; Next generation sequencing; Nodulation.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400783
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Draft genome sequence of Bradyrhizobium sp. strain BR 3267, an elite strain recommended for cowpea inoculation in Brazil BJM
Simões-Araújo,Jean Luiz; Leite,Jakson; Passos,Samuel Ribeiro; Xavier,Gustavo Ribeiro; Rumjanek,Norma Gouvêa; Zilli,Jerri Édson.
Abstract The strain BR 3267 is a nitrogen-fixing symbiotic bacteria isolated from soil of semi-arid area of Brazilian Northeast using cowpea as the trap plant. This strain is used as commercial inoculant for cowpea and presents high efficient in nitrogen fixation as consequence of its adaptation potential to semi-arid conditions. We report here the draft genome sequence of Bradyrhizobium sp. strain BR 3267, an elite bacterium used as inoculant for cowpea. Whole genome sequencing of BR 3267 using Illumina MiSeq sequencing technology has 55 scaffolds with a total genome size of 7,904,309 bp and C+G 63%. Annotation was added by the RAST prokaryotic genome annotation service and has shown 7314 coding sequences and 52 RNA genes.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Biological nitrogen fixation; Next generation sequencing; Nodulation; Semi-arid region.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400781
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Effect of point mutations on Herbaspirillum seropedicae NifA activity BJMBR
Aquino,B.; Stefanello,A.A.; Oliveira,M.A.S.; Pedrosa,F.O.; Souza,E.M.; Monteiro,R.A.; Chubatsu,L.S..
NifA is the transcriptional activator of the nif genes in Proteobacteria. It is usually regulated by nitrogen and oxygen, allowing biological nitrogen fixation to occur under appropriate conditions. NifA proteins have a typical three-domain structure, including a regulatory N-terminal GAF domain, which is involved in control by fixed nitrogen and not strictly required for activity, a catalytic AAA+ central domain, which catalyzes open complex formation, and a C-terminal domain involved in DNA-binding. In Herbaspirillum seropedicae, a β-proteobacterium capable of colonizing Graminae of agricultural importance, NifA regulation by ammonium involves its N-terminal GAF domain and the signal transduction protein GlnK. When the GAF domain is removed, the protein...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological nitrogen fixation; Herbaspirillum seropedicae; NifA.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800683
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