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Registros recuperados: 6
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Étude de la formation d'adduits et de 8-oxodguo à l'ADN de moule, Mytilus sp: perspectives d'utilisation dans le milieu marin en tant que biomarqueurs de génotoxicité des hydrocarbures aromatiques polycycliques ArchiMer
Akcha, Farida.
In the aim to develop biomarkers of genotoxicity for the biomonitoring of the marine environment,the formation of DNA adducts and oxidized DNA bases was studied for the sentinel organism, Mytilus sp, following exposure to B[a]P via the trophic supply. DNA adducts and 8-oxo-7,8-dihydro-2'-désoxyguanosine (8-oxodGuo) were respectively measured by 32P post-labeling technique and high performance liquid chromatography coupled to electrochemical detection. The developped model of exposure was validated for the realisation of toxicological studies in Mytilus sp. The bioavailability of B[a]P for the exposed organisms and its biotransformation resulted in variations in most of the measured enzymatic biomarkers of exposure. Such an exposure allowed the expression...
Tipo: Text Palavras-chave: Moule; B[a]P; Adduits; 8-oxodGuo; Biomarqueurs; Mussel; B[a]P; Adducts; 8-oxodGuo; Biomarkers.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00076/18770/16349.pdf
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Etude des variations saisonnières de différents biomarqueurs, chez la moule Mytilus galloprovincialis. Relation avec certains paramètres physiques, chimiques et physiologiques ArchiMer
Bodin, Nathalie.
The aim of this study was to evaluate the seasonal variations of six biomarkers on Mytilus galloprovincialis, using comparison with physiological index and chemical contamination levels on two sites of  Mediterranean sea. The seasonal variations of acetylcholinesterase (AChE), DNA adducts, benzo(a)pyrene hydroxylase (BPH), heat-shock proteins (HSP), metallothioneins (MT) and multixeniobiotic proteins (MXR) were estimated over a two-year period, on mussels which were sampled in Carteau (original site) and La Fourcade (transplanted organisms). Carteau, an industrial site, presents higher contamination levels (HAP, PCB, heavy metals) than La Fourcade, an urban area. The PAH profiles underline a strong spatio-temporal variability of levels and sources of...
Tipo: Text Palavras-chave: Biomonitoring; Mytilus galloprovincialis; Biomarqueurs; Variations saisonnières; Biomonitoring; Mytilus galloprovincialis; Biomarkers; Seasonal variations.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00443/55499/57042.pdf
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Etude d’outils d’évaluation de la contamination ChimiqUe dans les eaux de la ManchE - ECUME ArchiMer
Menet-nedelec, Florence; Halm-lemeille, Marie-pierre; Maheux, Frank; Pierre-duplessix, Olivier; Simon, Benjamin; Gonzalez, Jean-louis; Repecaud, Michel; Facq, Jean-valery.
L’étude ECUME était une opération destinée à tester localement dans les eaux côtières du littoral normand, des outils développés nationalement lors de nombreux travaux, et pressentis pour être utilisés lors des prochaines campagnes d’évaluation de la qualité chimique des masses d’eau littorales pour la DCE.  Ceci afin de déterminer la méthode la plus adaptée et opérationnelle de les déployer, et comparer leurs résultats entre eux et par rapport aux connaissances actuelles des niveaux de contamination. Trois sites ont été choisis : Chausey, baie des Veys et estuaire de Seine. Les résultats des analyses chimiques dans l’eau de mer grâce aux DGT, POCIS, SBSE, et dans les moules ont permis de confirmer le contraste du niveau de contamination en éléments traces...
Tipo: Text Palavras-chave: Contamination chimique; Chausey; Baie des Veys; Estuaire de Seine; DGT; POCIS; SBSE; Biomarqueurs; Stabilité lysosomale; Micronoyaux; Activité catalase; Métaux; Pesticides; Substances pharmaceutiques; HAP; PCB; Phtalates; Alkylphénols.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00434/54582/55966.pdf
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Fish as bioindicators of brackish ecosystem health: integrating biomarker responses and target pollutant concentrations ArchiMer
Corsi, I; Mariottini, M; Sensini, C; Lancini, L; Focardi, S.
Biological responses and contaminant levels in biological tissues were investigated in fish specimens of grass goby (Zosterisessor ophiocephalus) collected in spring 2000 in a moderately polluted brackish ecosystem, the Orbetello lagoon (southern coast of Tuscany, Italy). The complexity of harmful effects that contaminants may cause in fish over time was assessed. General condition indices (somatic liver index, and gonadal somatic index), cytochrome P450 enzymes, acetylcholinesterase activity and ovarian morphology were integrated with the content of organochlorines (OCs-hexachlorobenzene, DDTs and polychlorinated biphenyls) in liver and ovaries and p-nonylphenol (NP) and lower ethoxylate (NPE(1-2)) content in muscle and ovaries as well. Samples collected...
Tipo: Text Palavras-chave: Biomarqueurs; Lagunes; Poissons; Histologie des ovaires; Organochlorés; Biomarkers; Lagoon; Fish; Ovarian histology; Organochlorines; Nonylphenols.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00322/43293/43028.pdf
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La métabolomique et les biotoxines marines ArchiMer
Mondeguer, Florence.
Metabolomics is a new approach to assess the contamination of organisms, environmental compartments or foodstuffs, and the impact marine pollution, (natural or anthropogenic), can have on them. Through the analysis of the variation of the metabolite profiles present in the cell, tissues, organs or even the whole organism it is possible to define a physiological state, a stage of development, or the level and nature of a contamination. Generally, analysis of metabolites correlates to experimental design built around a clear biological or environmental question, needs to be supported by sensitive and reproducible tools, and requires access to libraries "house" or international. Often, the non-targeted approach allows for evaluation of many hypotheses. Thus,...
Tipo: Text Palavras-chave: Biotoxines; Biomarqueurs; Métabolomique; Microalgues; Moules; Spectrométrie de masse haute résolution (HRMS); Workflow.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00358/46892/46777.pdf
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Utilisation des biomarqueurs pour la caractérisation de l’état écotoxicologique des masses d’eau ArchiMer
Sanchez, Wilfried; Porcher, J.-m..
In the past 25 years, numerous biomarkers have been developed with the objective to apply them to aquatic ecosystem biomonitoring. Recently, the Water Framework Directive (WFD) of the European union specified monitoring programs required to assess the achievement of good chemical and ecological status for all water bodies by 2015. This article reviews the potential of biomarkers for ecotoxicological status assessment in WFD monitoring programmes based on previous national and international experiments.
Tipo: Text Palavras-chave: Écotoxicologie; Biomarqueurs; Biosurveillance; Directive cadre sur l’eau; Ecotoxicology; Biomarkers; Biomonitoring; Water Framework Directive.
Ano: 2009 URL: https://archimer.ifremer.fr/doc/00500/61183/64703.pdf
Registros recuperados: 6
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