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Assessment of toxicity of a biosurfactant from Candida sphaerica UCP 0995 cultivated with industrial residues in a bioreactor Electron. J. Biotechnol.
Bezerra de Souza Sobrinho,Humberto; de Luna,Juliana Moura; Rufino,Raquel Diniz; Figueiredo Porto,Ana Lúcia; Sarubbo,Leonie Asfora.
Background: The aim of the present study was to propose a low-cost method for the production of a biosurfactant by the yeast Candida sphaerica and assess its toxicity and phytotoxicity. The medium was formulated with distilled water supplemented with residue from a soy oil refinery (5%) and corn steep liquor (2.5%) as substrates. These two products were the sources of carbon and nitrogen as well as mineral elements to encourage the growth of the microorganism and production of a biosurfactant. Results: The isolated biosurfactant yield was 6.364 g/l. The biosurfactant exhibited an excellent ability to reduce surface tension (26 mN/m) and demonstrated no toxicity against seeds of Brassica oleracea, Chicoria intybus and Solanum gilo or the micro crustacean...
Tipo: Journal article Palavras-chave: Biosurfactant; Bioreactor; Candida sphaerica; Industrial residues; Toxicity.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400004
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Bioremediation of polycyclic aromatic hydrocarbon (PAH) compounds: (acenaphthene and fluorene) in water using indigenous bacterial species isolated from the Diep and Plankenburg rivers, Western Cape, South Africa BJM
Alegbeleye,Oluwadara Oluwaseun; Opeolu,Beatrice Olutoyin; Jackson,Vanessa.
Abstract This study was conducted to investigate the occurrence of PAH degrading microorganisms in two river systems in the Western Cape, South Africa and their ability to degrade two PAH compounds: acenaphthene and fluorene. A total of 19 bacterial isolates were obtained from the Diep and Plankenburg rivers among which four were identified as acenaphthene and fluorene degrading isolates. In simulated batch scale experiments, the optimum temperature for efficient degradation of both compounds was determined in a shaking incubator after 14 days, testing at 25 °C, 30 °C, 35 °C, 37 °C, 38 °C, 40 °C and 45 °C followed by experiments in a Stirred Tank Bioreactor using optimum temperature profiles from the batch experiment results. All experiments were run...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polycyclic aromatic hydrocarbons (PAHs); Bioremediation; Bioreactor; PAH degrading microorganisms.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200314
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Caffeine degradation by Rhizopus delemar in packed bed column bioreactor using coffee husk as substrate BJM
Tagliari,Cristiane Vanessa; Sanson,Raquel K.; Zanette,André; Franco,Telma Teixeira; Soccol,Carlos Ricardo.
Various microorganisms including bacteria, yeast and fungi can degrade caffeine. There are few publications about caffeine degradation pathway in filamentous fungi, mainly by solid-state fermentation (SSF). Studies were carried out on degradation of caffeine and their metabolites by filamentous fungi in SSF using coffee husk as substrate. The purpose of this work was to investigate the caffeine degradation pathway by Rhizopus delemar in packed bed column fermenter and to compare this degradation metabolism with glass flasks fermentation. The methylxanthines were quantified by HPLC analysis. The experiments were realized with the optimized conditions in previous experiments: pH 6.5, 28ºC, inoculation rate 10(6) spores/g substrate, aeration rate 60 mL/min...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Decaffeination; Fermentation; Caffeine; Theophylline; Bioreactor; Filamentous fungi.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500035
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Cattleya walkeriana growth in different micropropagation systems Ciência Rural
Moreira,André Luís; Silva,Adriano Bortolotti da; Santos,Aline; Reis,Caroline Oliveira dos; Landgraf,Paulo Roberto Correa.
The aim of the present research was to verify the in vitro growth of orchids in different systems of micropropagation, being cultivated in a bioreactor, with natural ventilation and conventional systems. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. After 8 months, seedlings with 1 cm of length were placed in a culture vessel according to the treatments, which counted with two micropropagation systems (conventional and natural ventilation) in three media of culture (liquid, solid with 5 or 6g L-1 of agar). Two additional treatments in bioreactor of temporary and continuous immersion were performed. The design was entirely randomized (ERD), consisting of a 2x3 factorial with two additional treatments, totaling 8...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioreactor; Temporary immersion; Liquid medium; Natural ventilation; In vitro culture.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001000012
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Continuous enrichment cultures: insights into prokaryotic diversity and metabolic interactions in deep-sea vent chimneys ArchiMer
Postec, Anne; Lesongeur, Francoise; Pignet, Patricia; Ollivier, B; Querellou, Joel; Godfroy, Anne.
The prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. Cultures were conducted at 60 degrees C and pH 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the Rainbow field, Mid-Atlantic Ridge, at 2,275 m depth. To assess the relevance of both culture methods, bacterial and archaeal diversity was studied using cloning and sequencing, DGGE, and whole-cell hybridisation of 16S rRNA genes. Sequences of heterotrophic microorganisms belonging to the genera Marinitoga,...
Tipo: Text Palavras-chave: Metabolic interactions; 16S rRNA gene; Thermophiles; Bioreactor; Continuous enrichment cultures; Deep sea hydrothermal vent; Microbial diversity.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3885.pdf
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Development of Fed-Batch Cultivation Strategy for Efficient Oxytetracycline Production by Streptomyces rimosus at Semi-Industrial Scale BABT
Elsayed,Elsayed Ahmed; Omar,Hoda Gamal; El-Enshasy,Hesham Ali.
ABSTRACTOxytetracycline (OTC) production byStreptomyces rimosus was studied in batch and fed-batch cultures in shake flask and bioreactor levels using semi-defined medium. First, the effect of glucose concentration on OTC production and growth kinetics was studied intensively. The optimal glucose concentration in the medium was 15 g/L. Higher glucose concentrations supported higher biomass production by less volumetric and specific antibiotic production. Based on these data, cultivations were carried out at semi-industrial scale 15 L bioreactor in batch culture. At bioreactor level, cell growth and OTC production were higher compared to the shake flask culture by about 18 and 38%, respectively. During the bioreactor cultivation, glucose was totally...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oxytetracycline; Streptomyces rimosus; Bioreactor; Fed-batch cultivation; Feed-back control.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500676
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EFFECT OF AERATION AND RECIRCULATION IN THE REMOVAL OF NITROGEN AND CHEMICAL OXYGEN DEMAND FROM SANITARY SEWAGE IN A STRUCTURED BED REACTOR REA
Leick,Sheilla A.; Oliveira,João G B. De; Tavares,Karoline A.; Lopes,Deize D.; Barana,Ana C..
ABSTRACT This study aimed to evaluate a structured bed reactor with intermittent aeration in the simultaneous removal of Chemical Oxygen Demand (COD) and Total Nitrogen (TN) from sanitary sewage. The reactor was operated for 339 days with a Hydraulic Retention Time (HRT) of 12 hours, temperature of 30 ± 1°C and continuous feeding. Nine trials were carried out in which three different effluent recirculation flow (Qr), 3, 2 and 1 time the incoming flow, were evaluated in three different aeration times, of 1h, 2h, and 3h, in 3 hour cycles. The results showed that different recirculation flows and aeration times did not influence the removal of COD, which reached 89%±12. It was observed that the efficiency of TN removal was higher in the tests with higher...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioreactor; Wastewater; Denitrification; Nitrification; SND (Simultaneous Nitrification and Denitrification).
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162017000601236
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Effect of variation of environmental conditions on the microbial communities of deep-sea vent chimneys, cultured in a bioreactor ArchiMer
Byrne, Nathalie; Lesongeur, Francoise; Bienvenu, Nadege; Geslin, Claire; Alain, Karine; Prieur, Daniel; Godfroy, Anne.
Both cultivation and molecular techniques were used to investigate the microbial diversity and dynamic of a deep-sea vent chimney. The enrichment cultures performed in a gas-lift bioreactor were inoculated with a black smoker chimney sample collected on TAG site on the mid-Atlantic ridge. To mimic as close as possible environmental conditions, the cultures were performed in oligotrophic medium with nitrogen, hydrogen and carbon dioxide (N-2/H-2/CO2) gas sweeping. Also, the temperature was first settled at a temperature of 85A degrees C and colloidal sulphur was added. Then, the temperature was lowered to 60A degrees C and sulphur was omitted. Archaeal and bacterial diversity was studied in both culture and natural samples. Through 16S rRNA gene sequences...
Tipo: Text Palavras-chave: 16S rRNA genes; Oligotrophic condition; Environmental condition; Bioreactor; Continuous enrichment culture; Microbial diversity; Deep sea hydrothermal vent.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6920.pdf
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Evaluation of the microbial diversity in sequencing batch reactor treating linear alkylbenzene sulfonate under denitrifying and mesophilic conditions using swine sludge as inoculum BABT
Duarte,Iolanda Cristina Silveira; Oliveira,Lorena Lima de; Okada,Dagoberto Yukio; Prado,Pierre Ferreira do; Varesche,Maria Bernadete Amâncio.
The objective of this study was to evaluate the degradation of Linear Alkylbenzene Sulfonate (LAS) in anaerobic sequencing batch reactor (ASBR) under denitrifying conditions using swine sludge as inoculum. The reactor was operated for 104 days with synthetic substrate containing nitrate, and LAS was added later (22 mg/L). Considering the added mass of the LAS, the adsorbed mass in the sludge and discarded along with the effluent, degradation of the surfactant at the end of operation was 87%, removal of chemical oxygen demand was 86% and nitrate was 98%. The bacterial community was evaluated by cutting the bands and sequencing of polymerase chain reaction (PCR) fragments and denaturing gradient gel electrophoresis (DGGE). The sequences obtained were related...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Linear alkylbenzene sulfonate (LAS); Bioreactor; PCR/DGGE; Denitrified.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300326
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Genetic transformation studies and scale up of hairy root culture of Glycyrrhiza glabra in bioreactor Electron. J. Biotechnol.
Mehrotra,Shakti; Kumar Kukreja,Arun; Singh Khanuja,Suman Preet; Nath Mishra,Bhartendu.
The study was undertaken to induce hairy roots in Glycyrrhiza glabra in leaf explants and to optimize the nutritional requirement for its growth kinetics at shake flask and bioreactor level. Pathogenecity of Agrobacterium depends upon transformation ability of strain and age, type, and physiological state of explants. Agrobacterium rhizogenes strain K599 was used to infect leaf explants of G. glabra. Explants of different age groups were obtained from 2 to 5 weeks old in vitro grown cultures. Bacterial strain K599 could induce hairy roots in 3 and 4 weeks old leaf explants cultured on B5, MS, NB and WP basal semi-solid medium. Leaf explants of 2 and 5 weeks old culture were not responsive to bacterial infection in terms of hairy root induction. Maximum...
Tipo: Journal article Palavras-chave: Agrobacterium; Bioreactor; Glycyrrhiza; Scale-up; Transformed roots.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200009
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Increasing Scopolamine content in Hairy Roots of Atropa belladonna using Bioreactor BABT
Habibi,Peyman; Piri,Khosro; Deljo,Ali; Moghadam,Yaser Ahmadi; Ghiasvand,Taiebeh.
The aim of this study was to use the, hairy root system for increasing the scopolamine content in Atropa belladonna. Agrobacterium rhizogenes ATCC15834 was utilized to produce hairy roots. The culture was carried out in a 1.5-l bioreactor using the inoculum size of 0.5 g fr. wt of 10-day-old hairy roots and various parameters, including agitation, aeration, conductivity and the consumption of sucrose were evaluated. Results revealed that the highest amount of scopolamine production (1.59 mg/g-1 dry wt) occurred in the bioreactor with aeration and agitation 1.25 vvm (volume per volume per minute) and 70 rpm, respectively. Study of conductivity and the consumption of sucrose showed that the highest amount of sucrose consumption and the highest amount of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atropa belladonna; Hairy root; Scopolamine; Bioreactor.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200166
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Monascus pigment production in bioreactor using a co-product of biodiesel as substrate Ciênc. Tecnol. Aliment.
Bühler,Rose Marie Meinicke; Dutra,Anderson Cesar; Vendruscolo,Francielo; Moritz,Denise Esteves; Ninow,Jorge Luiz.
The study and use of natural pigments in food industries have increased in recent years due to the toxicity presented by artificial pigments. Monascus ruber is a filamentous fungus that produces red, orange, and yellow pigments under different growing conditions. The growth of health food market has increased in parallel with the growth in biofuels production, such as biodiesel, which generates a concomitant increase in the production of glycerin that can be used in bioprocesses. The objective of this study was to use glycerin and glucose as substrates in the production of natural pigments in a bioreactor. The culture of Monascus ruber was carried out in a Bioflo III reactor with 4 L of working volume and pH, temperature, aeration, and agitation control....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monascus ruber; Glycerin; Bioreactor.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000500002
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Optimisation of growth conditions for continuous culture of the hyperthermophilic archaeon Thermococcus hydrothermalis and development of sulphur-free defined and minimal media ArchiMer
Postec, Anne; Pignet, Patricia; Cueff-gauchard, Valerie; Schmitt, Anne; Querellou, Joel; Godfroy, Anne.
The hyperthermophilic archaeon Thermococcus hydrothermalis was cultivated in continuous culture in a gas-lift bioreactor in the absence of elemental sulphur on both proteinaceous and maltose-containing media. Optimal conditions (pH, temperature and gas flow rate), determined on complex media that yielded maximal growth rate and maximal steady state cell density, were obtained at 80degreesC, pH 6 and gas sparging at 0.2 v v(-1) min(-1). Higher steady state cell densities were obtained on a medium containing maltose and yeast extract. In order to design a defined and minimal media, the nutritional requirements of T hydrothermalis were then investigated using continuous culture in the absence of elemental sulphur in the gas-lift bioreactor. First, the complex...
Tipo: Text Palavras-chave: Bioreactor; Defined and minimal media; Culture optimisation; Gas lift bioreactor; Thermococcus hydrothermalis.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-365.pdf
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Optimization of growth and bacteriocin activity of the food bioprotective Carnobacterium divergens V41 in an animal origin protein free medium ArchiMer
Brillet-viel, Anne; Pilet, Marie-france; Courcoux, Philippe; Prevost, Hervé; Leroi, Francoise.
Optimization of Carnobacterium divergens V41 growth and bacteriocin activity in a culture medium deprived of animal protein, needs for food bioprotection, was performed by using a statistical approach. In a screening experiment, twelve factors (pH, temperature, carbohydrates, NaCl, yeast extract, soy peptone, sodium acetate, ammonium citrate, magnesium sulphate, manganese sulphate, ascorbic acid and thiamine) were tested for their influence on the maximal growth and bacteriocin activity using a two-level incomplete factorial design with 192 experiments performed in microtiter plate wells. Based on results, a basic medium was developed and three variables (pH, temperature and carbohydrates concentration) were selected for a scale-up study in bioreactor. A...
Tipo: Text Palavras-chave: Optimization; Carnobacterium; Bacteriocin; Experimental design; Bioreactor; Growth; Cold-smoked salmon.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00345/45615/45236.pdf
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Optimization of polyethylenimine-mediated transient transfection using response surface methodology design Electron. J. Biotechnol.
Fang,Qiangyi; Shen,Bingqian.
Response surface methodology was undertaken to optimize the polyethylenimine-mediated transient transfection of suspension cultured HEK 293-F cells. A total of 15 combinations were designed according to Box-Behnken design to identify the effects of DNA concentration, polyethylenimine concentration and incubation time on transient transfection efficiency. The highest integral optic density of green fluorescent protein presenting r-protein yield was accessed using a DNA concentration of 1.75 µg/mL, a polyethylenimine concentration of 10.5 µg/mL, and an incubation time of 11.8 min. Analysis of variance demonstrated that the experimental values fit well with a quadratic model. The RSM-optimized transient transfection resulted in greater production of human...
Tipo: Journal article Palavras-chave: Bioreactor; Human tissue kallikrein; Transient gene expression.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500010
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Performance of the constructed wetland system for the treatment of water from the corumbataí river BABT
Borges,Ana Kleiber Pessoa; Tauk-Tornisielo,Sâmia Maria; Domingos,Roberto Naves; Angelis,Dejanira de Franceschi de.
The aim of this work was to study the constructed wetland system for the treatment of water from the Corumbataí river simulated on a laboratory scale. The parameters analyzed at different points of the system were ammonia, biochemical demand for oxygen (BDO), chemical demand for oxygen (CDO), chlorides, apparent color, conductivity, dissolved oxygen, magnesium (Mg), sodium (Na), potassium (K), silicon (Si), total phosphorous, total coliforms and Escherichia coli, total dissolved solids (TDS), turbidity, and macrophyte biomass. The results demonstrated that this alternative water treatment system was effective in removing the microorganisms (total coliforms and E. coli), among other parameters analyzed, for varying periods of the treatment, promoting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquatic macrophytes; Filtering soil; Microfiltration; Bioreactor; Microorganisms.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600024
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Plant regeneration via indirect somatic embryogenesis and optimisation of genetic transformation in Coffea arabica L. cvs. Caturra and Catuaí Electron. J. Biotechnol.
Gatica-Arias,Andrés M; Arrieta-Espinoza,Griselda; Espinoza Esquivel,Ana M.
A protocol for Coffea arabica L. cvs. Caturra and Catuaí plant regeneration via indirect somatic embryogenesis (ISE) was established. Furthermore, a biolistic mediated genetic transformation protocol was optimized for Catuaí callus aggregates. Maximum callus induction was obtained when Caturra (87%) and Catuaí (67%) leaves were cultured on Murashige and Skoog medium with 18.56 µM kinetin and 4.52 µM2,4-dichlorophenoxyacetic acid (2,4-D). Catuaí suspension cultures were established from embryogenic callus using liquid proliferation CP and Sli media and diffused light and darkness. The higher suspension cultures fresh weight was obtained using Erlenmeyer (1425.4 ± 354.9 mg) than Recipient for Automated Temporary Immersion System (RITA®) (518.6 ± 55.1 mg),...
Tipo: Journal article Palavras-chave: Bioreactor; Coffee; Genetic transformation; Somatic embryogenesis; Suspension cultures.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100010
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Production and characterization of an extracellular lipase from Candida guilliermondii BJM
Oliveira,Anne Caroline Defranceschi; Fernandes,Maria Luiza; Mariano,André Bellin.
Extracellular lipases from the endophytic yeast Candida guilliermondii isolated from castor leaves (Ricinus communis L.) were produced using low-cost raw materials such as agro-industrial residues and applying them in the esterification of oleic acid for evaluating their potential use in biodiesel production. After partial purification using ammonium sulfate, the enzyme was characterized and presented higher activity (26.8 ± 1.5 U mL-1) in the presence of 5 mmol L-1 NaCl at 30 ºC and pH 6.5. The production through submerged fermentation was formerly performed in 150 mL erlenmeyer flasks and, once the enzyme production was verified, assays in a 14 L bioreactor were conducted, obtaining 18 ± 1.4 U mL-1. The produced enzyme was applied in the oleic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular lipase; Endophytic yeast; Esterification; Submerged fermentation; Bioreactor.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400047
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Production of Poly-γ-Glutamate (PGA) Biopolymer by Batch and Semicontinuous Cultures of Immobilized Bacilluslicheniformis strain-R BJM
Berekaa,Mahmoud M.; El Aassar,Samy A.; El-Sayed,Samia M.; EL Borai,Aliaa M..
Production of Polyglutamate (PGA) biopolymer by immobilized Bacilluslicheniformis strain-R was intensively investigated. Preliminary experiments were carried out to address the most suitable immobilization methodology. Entrapment of Bacillus cells in alginate-agar led optimal PGA production (36.75 g/l), with 1.32- and 2.18-fold increase in comparison with alginate- or K-carrageenan-immobilized cells, respectively. During semicontinuous cultivation of agar-alginate gel-cell mixture, production of PGA by 10 ml mixture was increased from 2nd to 3rd run whereas, increased till the 4th run using 15ml mixture. Adsorption was the most suitable immobilization technique for production of PGA and the sponge cubes was the preferred matrix recording 43.2 g/l of PGA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Poly-g-glutamate; Bacillu slicheniformis; Immobilization; Bioreactor; Semicontinuous production.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400001
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Semi-scale production of PHAs from waste frying oil by Pseudomonas fluorescens S48 BJM
Gamal,Rawia F.; Abdelhady,Hemmat M.; Khodair,Taha A.; El-Tayeb,Tarek S.; Hassan,Enas A.; Aboutaleb,Khadiga A..
The present study aimed at developing a strategy to improve the volumetric production of PHAs by Pseudomonas fluorescens S48 using waste frying oil (WFO) as the sole carbon source. For this purpose, several cultivations were set up to steadily improve nutrients supply to attain high cell density and high biopolymer productivity. The production of PHAs was examined in a 14 L bioreactor as one-stage batch, two-stage batch, and high-cell-density fed-batch cultures. The highest value of polymer content in one-stage bioreactor was obtained after 60 h (33.7%). Whereas, the two-stage batch culture increased the polymer content to 50.1% after 54 h. High-cell-density (0.64 g/L) at continuous feeding rate 0.55 mL/l/h of WFO recorded the highest polymer content after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas fluorescens S48; Biopolymer; PHAs; Batch; Two-stage batch; High-celldensity fed batch; Bioreactor; Recovery.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200034
Registros recuperados: 22
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