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Caracterização molecular e divergência genética de Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) em diferentes culturas e locais de cultivo Neotropical Entomology
Fontes,Fernanda Von H M; Colombo,Carlos A; Lourenção,André L.
Knowledge on the genetic variation of populations of Bemisia tabaci (Genn.) can improve the understanding of genetic diversity found in their biotypes and, consequently, offer guidelines for its management. In this study, the molecular characterization was performed and genetic diversity data were obtained for this insect from three regions of Brazil on different crops [cotton and soybean (Mato Grosso - MT); cabbage (Distrito Federal - DF); soybean and potato (São Paulo - SP)], using RAPD markers. RAPD analysis indicated 80.6% polymorphic loci and the average genetic similarity obtained by the Jaccard coefficient was 0.67. The whitefly populations collected on potato (SP) and soybean (MT) had higher genetic diversity values (0.75 and 0.72, respectively)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Silverleaf whitefly; RAPD-PCR; Biotype; Genetic diversity; Squash silvering.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000200012
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Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycetemcomitans isolated from Brazilian patients with chronic periodontitis BJM
Gaetti-Jardim Jr.,Elerson; Wahasugui,Thais Cristiane; Tomazinho,Paulo Henrique; Marques,Márcia Martins; Nakano,Viviane; Avila-Campos,Mario Julio.
Aggregatibacter actinomycetemcomitans is an important etiologic agent of the periodontitis and is associated with extra-oral infections. In this study, the detection of the ltxA gene as well as the ltx promoter region from leukotoxic A. actinomycetemcomitans isolated from 50 Brazilian patients with periodontitis and 50 healthy subjects was performed. The leukotoxic activity on HL-60 cells was also evaluated. Leukotoxic activity was determined using a trypan blue exclusion method. The 530 bp deletion in the promoter region was evaluated by PCR using a PRO primer pair. A. actinomycetemcomitans was detected by culture and directly from crude subgingival biofilm by PCR using specific primers. By culture, A. actinomycetemcomitans was detected in nine (18%) of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotype; Leukotoxin; Periodontitis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400011
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Diversidade molecular entre populações de Spodoptera frugiperda no Brasil avaliada por marcadores AFLP. Repositório Alice
PINTO, C. C.; GRÜTZMACHER, A. D.; ROSA, A. P. S. A. da; MANICA-BERTO, R.; MENDES, S. M.; ARGE, L. W. P.; BORGES, C. T..
O objetivo deste trabalho foi avaliar a diversidade molecular, por meio de marcadores AFLP, de seis populações de Spodoptera frugiperda coletadas na cultura do milho, em diferentes regiões geográficas do Brasil. O DNA foi extraído de lagartas de quarto instar, e as reações de AFLP foram realizadas com sete combinações de oligonucleotídeos iniciadores. A partir das seis populações de S. frugiperda estudadas, foi identificado um grupo principal formado por três populações geneticamente mais relacionadas. As populações de S. frugiperda analisadas mostram alta variabilidade genética, com máximo de 58% de similaridade.
Tipo: Artigo de periódico Palavras-chave: Biotype; Fall armyworm; Molecular markers.; Genetic distance..
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1017873
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Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil Planta Daninha
Rocha,D.C.; Rodella,R.A.; Marino,C.L.; Martins,D..
This work aims to carry out a comparative analysis using RAPD molecular markers in four Commelina weed species from the state of Paraná and C. benghalensis populations from the states of Paraná and São Paulo, Brazil. The genomic plant DNA sample was extracted from the leaves, separated, randomly fragmented and amplified by PCR. Random amplified polymorphic DNA fragments (RAPD markers) were analyzed by using POPGENE statistical program. Eighty-five primer sequences were tested but only three were suitable as molecular markers producing 37 DNA polymorphic fragments for comparisons among four Commelina species and 22 polymorphic fragments for comparisons among C. benghalensis populations. The results showed that there were inter-specific and intra-specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotype; Commelinaceae; Molecular markers; Weeds.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582009000300001
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Growth Analysis of Chloris elata Planta Daninha
CORREIA,N.M.; RESENDE,I..
ABSTRACT: In Brazil, in agricultural areas where there is no establishment of cover crops in the off season, as well as in citrus orchards in the state of São Paulo, an increase in the infestation of Chloris elata has been observed. This is a perennial species, propagated by seeds and short rhizomes, erect and slightly caespitose. With the objective of evaluating the growth and development of plants from two accessions of C. elata, a greenhouse experiment was conducted. The experimental design was completely randomized, in a 2 (accessions of C. elata) x 15 (evaluation periods) factorial arrangement, with four replications. Fifteen destructive evaluations were performed 14, 21, 28, 35, 42, 49, 56, 63, 70, 77, 84, 91, 98, 112 and 126 days after sowing (DAS)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotype; Tall windmill grass; Plant development.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100328
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Hessian Fly, Mayetiola destructor (Say), Populations in the North of Tunisia: Virulence, Yield Loss Assessment and Phenological Data Chilean J. Agric. Res.
Makni,Hanem; Bouktila,Dhia; Mezghani,Maha; Makni,Mohamed.
Hessian fly, Mayetiola destructor (Say), is a destructive pest of wheat worldwide and an endemic pest in Tunisia. Two natural populations of this insect from the North of Tunisia were evaluated, in the field, for their virulence, based on response developed by bread wheat (Triticum aestivum L.) cultivars carrying H3, H5, H6, H7H8, H11, H13 and H16 resistance genes. H11, H13 and H16 showed a high effectiveness against both populations; therefore, their implication in Hessian fly breeding programs would be of interest. The level of infestation, as well as the yield loss, was assessed, based on the percentage of infested plants and variation in growth parameters due to infestation. The percentage of infested plants, over a 2-yr period in Mateur, averaged...
Tipo: Journal article Palavras-chave: Wheat; Biotype; Generations; Integrated pest management.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300009
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Morphofunctional characteristics of working mules in mountain areas of the Colombian Central Andes Animal Sciences
Mosquera, Juan Carlos Velasquez; Álvarez, Juan David Corrales; Jacometo, Carolina Bespalhok.
The application of morphofunctional measurements allows the selection of suitable animals to perform working activities and ensure that animal maximum capacities are not exceeded. Mules are commonly used by small farmers for agricultural work in mountain areas where the access is extremely difficult. We aimed to estimate the functionality indexes of working mules in mountain areas of the Colombian Central Andes. A total of 94 adult mules were evaluated for withers height, thoracic perimeter, body length, neck length, and body weight, which were used to determine the body index (BI), proportionality index (PI), and load carriage index (LCI). Descriptive statistics, analysis of correlations, and principal component analysis were performed. Males presented...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotype; Equus caballus x Equus asinus; Load carriage index; Morphological indices..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/46379
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Response of Three Chloris elata Populations to Herbicides Sprayed in Pre- and Post-Emergence Planta Daninha
CORREIA,N.M.; RESENDE,Í..
ABSTRACT: In agricultural areas of Brazil, where there is no establishment of cover crops in the off-season and in fruit orchards, an increase in Chloris elata infestations, syn. Chloris polydactyla, has been observed. With the purpose to evaluate the response of three populations (Itaberaí - GO, Matão - SP e Jaboticabal - SP) of C. elata to herbicides sprayed before and after emergence, four experiments were conducted. In the first one, the differential tolerance of three C. elata populations to the herbicide glyphosate (0, 0.18, 0.36, 0.72, 0.96, 1.44, 2.88, 5.76 and 11.52 kg a.e. ha-1) was studied. In the other experiments, the control of the three populations by herbicides applied in pre-emergence (clomazone, diclosulam, flumioxazin, isoxaflutole,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotype; Tall windmill grass; Chemical control; Weed.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100277
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Ribotyping, biotyping and capsular typing of Haemophilus influenzae strains isolated from patients in Campinas, southeast Brazil BJID
Lancellotti,Marcelo; Pace,Fernanda de; Stehling,Eliana Guedes; Villares,Maria Cecília Barisson; Brocchi,Marcelo; Silveira,Wanderley Dias da.
Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of an amplified 16S DNA region]. The strains were better discriminated by the ribotyping technique that used the 16S probe and by the combination of both techniques. Biotypes I and IV were the most common, followed by biotypes VI, VIII and III. Biotypes II and VII were not found. Most of the capsular samples were nontypable (89%), with capsular types a and b found in 2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Haemophilus influenzae; Characterization; Ribotyping; Serotype; Biotype.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500015
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การพัฒนาสายพันธุ์ข้าวต้านทานต่อความหลากหลายไบโอไทป์ ของเพลี้ยกระโดดสีน้ำตาล (Nilaparvata lugens (Stål)) Thai Agricultural
Jirapong Jairin; Pamorn Pattawatang; Varapong Chamarerk; Phikul Leelakud; Wanwisa Khunnasut; Watchalawalee Boonmee; Pimnapa Uttakavapee; Wanida Thueannadee.
Development of rice varieties conferring broad-spectrum and durable resistance against brown planthopper (BPH) is one of the major challenges facing the plant breeders. Idea has been generated in this study for incorporating resistance genes of ovicidal response and feeding inhibition into rice breeding lines. Our initial experiments focused on discovering the ovicidal trait in indica rice cultivars. We found that some weedy rice lines had the ovicidal resistance, causing 60% to 100% egg mortality. The egg mortality rate depends on the rice developmental stages and is the highest at the maximum tillering stage. This is the first report of the ovicidal trait in indica rice lines. The BPH resistance in weedy rice lines is not only mediated by the ovicidal...
Tipo: Collection Palavras-chave: Nilaparvata lugens; Rice; Biotype; Ovicidal resistance; Feeding inhibition; Weedy rice; Nilaparvata lugens; ข้าว; ไบโอไทป์; ความต้านทานจากการทำลายไข่; การยับยั้งการกินอาหาร; ข้าววัชพืช.
Ano: 2014 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5898
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การพัฒนาสายพันธุ์ข้าวต้านทานต่อความหลากหลายไบโอไทป์ของเพลี้ยกระโดดสีน้ำตาล (Nilaparvata lugens Stål) Thai Agricultural
Jirapong Jairin; Phikul Leelakud; Varapong Chamarerk; Pamorn Pattawatang; Watchalawalee Boonmee; Pimnapa Uttakavapee; Wanida Thueannadee.
Development of rice varieties conferring broad-spectrum and durable resistance against brown planthopper (BPH) is one of the major challenges facing the plant breeders. Idea has been generated in this study for incorporating resistance genes of ovicidal response and feeding inhibition into rice breeding lines. Our initial experiments focused on discovering the ovicidal trait in indica rice cultivars. We found that some weedy rice lines had the ovicidal resistance, causing 60% to 100% egg mortality. The egg mortality rate depends on the rice developmental stages and is the highest at the maximum tillering stage. This is the first report of the ovicidal trait in indica rice lines. The BPH resistance in weedy rice lines is not only mediated by the ovicidal...
Tipo: PhysicalObject Palavras-chave: Rice; Nilaparvata lugens; Biotype; Ovicidal resistance; Feeding inhibition; Weedy rice; ข้าว; ไบโอไทป์; ความต้านทานจากการทำลายไข่; การยับยั้งการกินอาหาร; ข้าววัชพืช; เพลี้ยกระโดดสีน้ำตาล; อัตราการตายของไข่.
Ano: 2014 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5624
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ความหลากหลายของแหล่งที่มาของเพลี้ยกระโดดสีน้ำตาลต่อความรุนแรงในการทำลายข้าวพันธุ์ต่างๆ Thai Agricultural
Orrachoan Poolsiri; Noppadol Kiripetch.
The brown planthopper (BPH) is one of the most important insect pests imposing limitations on the production of rice. The objective of this study is to investigate the effects of 32 BPH populations on 21 varieties of rice with differences in resistance to BPH while the TN1 variety used for a susceptible check. The previou by seedling box screening techniques and Standard Evaluation System for Rice. However, the limitation of this study was the analysis of data using only cluster analysis to group the sources of BPH and rice varieties but there was no detailed analysis for each group.
Tipo: PhysicalObject Palavras-chave: Virulence; Resistanct; BPH; Biotype; ข้าว; พันธุ์ต้านทาน; เพลี้ยกระโดดสีน้ำตาล; ความหลากหลายทางชีวชนิด; ความรุนแรง.
Ano: 2012 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5443
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ความหลากหลายทางพันธุกรรมและชีวชนิดของแมลงบั่วในประเทศไทย Thai Agricultural
Jirapong Jairin; Kanlaya Sansen; Sa-nguan Teangdeerith; Phikul Leelakud; Rungnapa Kawichai; Wantana Siratanasak; Nalinee Chiengwattana.
Amplified fragment length polymorphism (AFLP) and biotype-specific markers were used for identification of genetic diversity and biotypes of rice gall midge, Orseolia oryzae in Thailand. Pupae and adults of the gall midges were collected from 19 field locations of 12 provinces during 2006 - 2007. Total of 242 polymorphic AFLP bands were generated from 12 selective primer pairs. Cluster analysis, performed by the UPGMA, separated the gall midge populations into four distinct groups. To identify the biotype, five specific-biotype markers were used to characterize the collected gall midges. Two biotypes of the gall midges, biotype 2 and 5, were identified from all populations used in this research. The results showed that the genetic diversity was not...
Tipo: Collection Palavras-chave: Orseolia oryzae; Rice gall midge; Biotype; Genetic diversity; AFLP; ข้าว; แมลงบั่ว; การจำแนกชีวชนิด; ความหลากหลายทางพันธุกรรม; เอเอฟแอลพี; โมเลกุลเครื่องหมาย; ประเทศไทย.
Ano: 2009 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5092
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ความแตกต่างในความรุนแรงของเพลี้ยกระโดดสีน้ำตาลต่อการเข้าทำลายพันธุ์ข้าว Thai Agricultural
Jate Kotcharerk; Phamorn Pattawatang.
Evaluation of the ability of rice varieties in resistance to brown plant hopper was performed using seed box screen test. The brown plant hopers (BPH) were collected from rice paddy fields in Phitsanulok, Chainat, and Ang Thong Province. From cluster analysis at level 0.8, there were three different groups classified by the different level of rice plant damage after treated by the insects. The three different level of damage were graded by the ability in resistance to the BPH as resistance, moderately resistance and susceptible. Considering in biotype of the BPH, the results indicated that there were variety of BPH biotypes in each samples collected from the three different provinces. Mainly, the biotype 4 and 5 were indentified in these samples. However,...
Tipo: PhysicalObject Palavras-chave: Brown plant hopper; Biotype; Virulence; Paddy field; Rice variety; Resistance varieties; Nilaparvata lugens; Outbreak; Pest resistance; เพลี้ยกระโดดสีน้ำตาล; ชีวชนิด; ปฏิกิริยาความรุนแรง; จังหวัดพิษณุโลก; พันธุ์ต้านทาน; พันธุ์ข้าว; นาข้าว; ยีนต้านทานแมลงศัตรูพืช; การถ่ายทอดทางพันธุกรรม; การระบาดของแมลงศัตรูพืช; ปฏิกิริยาความรุนแรง.
Ano: 2014 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5681
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ปฏิกิริยาของข้าวเจ้าสายพันธุ์ PRE01017-10-1-1-1 ต่อแมลงบั่วในเขตภาคเหนือตอนบนในสภาพโรงเรือนปฏิบัติการ Thai Agricultural
Pannipa Yajai; Preeda Siangyai.
Reaction of PRE01017-10-1-1-1 rice promising line to gall midge (GM) resistance was evaluated in green house nursery at Phrae Rice Research Center during 2007-201. It was found that this promising line showed resistance (R) to high resistance (HR) to GM which collected from 5 areas, i.e., Mae Hong Son, Chiang Mai, Chiang Rai, Nan, and Phrae provinces. Furthermore, in 2012, the biotype of gall midge from 5 areas were studied and found that they were divided into two biotypes: 1) the gall midge collected from Mae Hong Son and Chiang Mai, which PRE01017-10-1-1-1 showed highly resistant (HR) score to GM, and 2) the gall midge collected from Chiang Rai, Nan and Phrae which PRE01017-10-1-1-1 showed resistant (R) score to GM. The mechanism of resistance in...
Tipo: PhysicalObject Palavras-chave: Rice gall midge; Biotype; Antibiosis; PRE01017-10-1-1-1; Rice promising line; ข้าว; ชีวชนิด; กลไกการต้านทาน; ข้าวสายพันธุ์ PRE01017-10-1-1-1; ข้าวเจ้าสายพันธุ์ดีเด่น; แมลงบั่ว; สภาพโรงเรือนปฏิบัติการ; เขตภาคเหนือตอนบน.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5528
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