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| Registros recuperados: 18 | |
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| Araújo,A.B.; Vianna,E.E.S.; Berne,M.E.A.. |
| Trypanosoma cruzi, the causal agent of Chagas' Disease, is a widely spread protozoa in America. Blood transfusion is the secondly most important way of acquiring the infection. In blood banks, tests are performed to eliminate potentially infected blood. This study aimed to evaluate the positivity for T. cruzi in blood samples of donor's candidates in Southern Brazil. The study was based on a sampling containing all blood donors of Hemopel - a Pelotas City Blood Center, Rio Grande do Sul State, Brazil, from 2004 to 2005. Serological study was performed using ELISA Chagatest. Sampling containing values ± 20% cut off were evaluated using ELISA Chagatek, ELISA Alka/Adaltis, IHA Chagatest and IIF Imunocruzi. TESA-Blot was used as a confirmatory procedure in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma cruzi; Blood donors; Serological screening. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600007 |
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| Felippe,MJDB; Meira,DA. |
| Hepatitis C is transmitted primarily parenterally by contaminated blood and is often associated with: intravenous drug abuse, invasive procedures, blood transfusions, acupuncture, tattooing, and alcohol and tobacco use. This study aimed to quantify and evaluate the risk factors among blood donors, volunteer blood donors and replacement individuals, infected or not by the C virus. The main transmission routes of C virus were identified in 55 men and 25 women (GI) monitored by the Ambulatory Unit of the Department of Tropical Diseases, Botucatu Medical School, and in 24 men and 26 women (GII), all active blood donors at the Bauru State Hospital Transfusional Agency. Both groups were similar in: tobacco and alcohol consumption, sexual behavior, tattooing and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis C; Risk factors; Blood donors; Injectable drugs; Tobacco; Alcohol; Marijuana; Sexual behavior. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100010 |
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| Lindenberg,Andréa de Siqueira Campos; Motta-Castro,Ana Rita Coimbra; Puga,Marco Antonio; Ortiz Tanaka,Tayana Serpa; Torres,Marina Sawada; Fernandes-Fitts,Sonia Maria; Cunha,Rivaldo Venancio. |
| Background The aim of the present study was to estimate hepatitis B virus seroprevalence among first-time blood donors in the city of Campo Grande, Mato Grosso do Sul State, in the central-western region of Brazil. Findings A retrospective analysis of first-time voluntary blood donor records, from January 2010 to December 2010, was conducted at the Hematology Center of Mato Grosso do Sul. The prevalence of the HBsAg and anti-HBc serological markers and their respective 95% confidence intervals were calculated. Chi-square analysis was performed between the seroprevalence previously found in 2001 and the one determined by the current study. Results were considered statistically significant if p < 0.05. Among 8,840 subjects, 269 (3.04%, 95% CI: 2.7-3.4)... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Hepatitis B virus; Prevalence; Blood donors. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100402 |
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| Speranza,F.A.B.; Ishii,S.K.; Hirata Jr.,R.; Mattos-Guaraldi,A.L.; Milagres,L.G.. |
| Serologic data on diseases that are preventable by vaccines are necessary to evaluate the success of immunization programs and to identify susceptible subgroups. In the present study, we determined serum IgG levels against diphtheria toxin of military and civilian blood donors (N = 75; 69.3% males and 30.7% females) aged 18-64 years, from the Brazilian Army Biology Institute, Rio de Janeiro, using a commercial diphtheria kit (Diphtheria IgG ELISA; IBL, Germany). Most (63%) unprotected military donors were from the older age group of 41 to 64 years. In contrast, the majority (71%) of young military donors (18 to 30 years) were fully protected. About half of the military donors aged 31 to 40 years were protected against diphtheria. Among the civilians, about... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Diphtheria protective antibodies; Military personnel; Blood donors; Age influence. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100017 |
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| Cunha,L.K.H.; Kashima,S.; Amarante,M.F.C.; Haddad,R.; Rodrigues,E.S.; Silva,K.L.T.; Lima,T.A.; Castro,D.B.; Brito,F.C.; Almeida,E.G.; Covas,D.T.; Malheiro,A.. |
| Few studies have reported the molecular epidemiological characterization of HIV-1 in the Northern region of Brazil. The present study reports the molecular and epidemiological characterization of 31 HIV-1 isolates from blood donors from the State of Amazonas who donated blood between April 2006 and March 2007. Serum/plasma samples from all donors were screened for HIV antibodies by ELISA and the results confirmed by Western blot analysis. Genomic DNA was extracted from the buffy coat using the Super Quik-Gene-DNA Isolation kit. Nested PCR was performed on the env, gag, and pol regions of HIV-1 using the Gene Amp PCR System 9700. Sequencing reactions were performed using the inner PCR primers and the DYEnamic™ ET Dye Terminator Kit, and phylogenetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Subtypes; Phylogenetic analysis; Blood donors; Molecular and epidemiological characterization. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200003 |
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| Spada,Celso; Souza,Marli Adelina; Treitinger,Arício. |
| The HIV, in hemotheraphy, may be transmitted by erythrocyte, platelets, crioprecipitated, frozen fresh plasma and possibly, by other blood components. Appropriate legislations for this new reality were elaborated normatizing the hemotheraphy practices in Brazil, creating a set of procedures and actions aiming at guaranteeing the quality of the blood, during, during the whole process. However, the residual risk remains, and it can be calculated as a product of the incidence and period of the immunological window. The objetive of the present study was to determine the rate of residual risk of the HIV blood transmission, in the blood donors from the Mountain Region of Santa Catarina. In order to calculate the residual risk of the HIV markers, 4,857 donors of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV; Blood donors; Residual risk; Transfusion security; Immunological window. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000600006 |
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| Acar,Ali; Kemahli,Sabri; Altunay,Husnu; Kosan,Erdogan; Oncul,Oral; Gorenek,Levent; Cavuslu,Saban. |
| The national blood transfusion policies have been changed significantly in recent years in Turkey. The purpose of this study was to determine the prevalence of HBV, HCV, and HIV in blood donors at the Red Crescent Center in Istanbul and to evaluate the effect of changes in the national blood transfusion policies on the prevalence of these infections. The screening results of 72695 blood donations at the Red Crescent Center in Istanbul between January and December 2007 were evaluated retrospectively. HBsAg, anti-HCV, and anti-HIV-1/2 were screened by microparticle enzyme immunoassay (MEIA) method. Samples found to be positive for anti-HIV 1/2 and anti-HCV were confirmed by Inno-Lia HCV Ab III and Inno-Lia HIV I/II Score, respectively. The seropositivity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HBV; HCV; HIV; Seroprevalence; Blood donors; Turkey. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100009 |
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| Santos,P.C.J.L.; Cançado,R.D.; Terada,C.T.; Rostelato,S.; Gonzales,I.; Hirata,R.D.C.; Hirata,M.H.; Chiattone,C.S.; Guerra-Shinohara,E.M.. |
| Mutations of the HFE and TFR2 genes have been associated with iron overload. HFE and TFR2 mutations were assessed in blood donors, and the relationship with iron status was evaluated. Subjects (N = 542) were recruited at the Hemocentro da Santa Casa de São Paulo, São Paulo, Brazil. Iron status was not influenced by HFE mutations in women and was independent of blood donation frequency. In contrast, men carrying the HFE 282CY genotype had lower total iron-binding capacity (TIBC) than HFE 282CC genotype carriers. Men who donated blood for the first time and were carriers of the HFE 282CY genotype had higher transferrin saturation values and lower TIBC concentrations than those with the homozygous wild genotype for the HFE C282Y mutation. Moreover, in this... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HFE; TFR2; Gene mutations; Blood donors; Iron status. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100015 |
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| TORRES,ALEX J.L.; CISNEIROS,PATRÍCIA; GUEDES,ROSA; GRASSI,MARIA FERNANDA R.; MEYER,ROBERTO; BENDICHO,MARIA T.; LOPES,TAIS G.S.L.; FÉLIX,GABRIELA; NETTO,EDUARDO M.; BRITES,CARLOS; ABE-SANDES,KIYOKO; BRANDÃO,CLAUDIO; ALCANTARA-NEVES,NEUZA; FREIRE,SONGELI M.. |
| The reference intervals for leukocytes and lymphocytes currently used by most clinical laboratories present limitations as they are primarily derived from individuals of North American and European origin. The objective this study was to determine reference values for peripheral blood B lymphocytes, T lymphocyte subsets (CD4+, CD8+, naïve, memory, regulatory, TCRαβ and TCRγδ+) and NK cells from blood donors in Salvador-Bahia, Brazil. Results: The proportion of included male subjects was 73.7% and the median ages of males (34) and females (35) were found to be similar. Absolute counts total lymphocytes subsets to both gender was 1,956 (1,060-4,186) cells and relative values 34%. The T CD4+ and T CD8+ lymphocytes relative values was 51% (20-62) and 24%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lymphocyte subsets; Reference values; Flow cytometry; Brazil; Blood donors. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000201019 |
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| Kinoshita-Yanaga,Andréa Tieko; Bértoli,Marta; Martins,Vanessa Aparecida; Mizutani,Angélica Sayuri; Toledo,Max Jean de Ornelas; Araújo,Silvana Marques de; Gomes,Mônica Lúcia. |
| The objective of this study was to evaluate, through blood culture and PCR, the results of the ELISA for Chagas' disease in the screening of blood donors in the public blood-supply network of the state of Paraná, Brazil, and to map the epidemiological profile of the donors with respect to their risk of infection by Trypanosoma cruzi. The negative and positive results of the ELISA were confirmed by blood culture and PCR for 190/191 individuals (99.5%). For one individual (0.5%), the ELISA was inconclusive, blood culture and IIF were negative, and IHA and PCR positive. Three individuals (1.6%) were positive for T. cruzi on all the tests. Donors were predominantly female, and natives of Paraná, of rural origin, had observed or been informed of the presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma cruzi/detection; ELISA/use for screening; Blood donors; Blood culture; PCR; Blood bank/screened donors. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100007 |
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| Haghighi,Malihe Fathi; Seyyedi,Noorossadat; Farhadi,Ali; Zare,Farahnaz; Kasraian,Leila; Dehbidi,Gholam Reza Refiei; Ranjbaran,Reza; Behzad-Behbahani,Abbas. |
| ABSTRACT Objectives: To investigate the prevalence of human polyomavirus (BK and JC viruses) infection in peripheral blood mononuclear cells of healthy blood donors. Methods: The study included 250 healthy blood donors. Five-milliliter blood was drawn into sterile EDTA tubes and PBMCs were isolated from whole blood. The isolated PBMCs were counted and stored at −70 °C for future investigation. DNA was extracted and subjected to simple, sensitive and specific semi-nested PCR as well as QPCR using both general and specific primers for different assays. Results: Of 250 blood samples, 66 (26.4%) were positive for BKV DNA (146-34,514 copies/106 cells). JC DNA was found in 45 (18%) blood samples (65-21,250 copies/106 cells). Co-infection with these viruses... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood donors; Leukocytes; Mononuclear; Polyomavirus; Real-time polymerase chain reaction. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100022 |
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| Torres,A.J.L.; Angelo,A.L.D.; Netto,E.M.; Sampaio,G.P.; Souza,D.F.C.; Inocêncio,L.A.; Lemos,J.A.R.; Brites,C.. |
| This study defined the normal variation range for different subsets of T-lymphocyte cells count in two different Brazilian regions. We analysed the T-lymphocytes subpopulations (CD3+, CD4+, CD8+) in blood donors of two Brazilian cities, located in North (Belem, capital state of Para, indian background) and Northeast (Salvador, capital state od Bahia, African background) regions of Brazil. Results were compared according to gender, stress level (sleep time lower than 8 hours/day), smoking, and alcohol intake. Lymphocytes subpopulations were measured by flow cytometry. Five hundred twenty-six blood donors from two Brazilians cities participated in the study: 450 samples from Bahia and 76 samples from Pará. Most (60%) were men, 59% reported alcohol intake,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CD4; CD8; Blood donors; Reference values; Brazil. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300013 |
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| Andrade,Elisabete; Rocha,Daniele; Fontana-Maurell,Marcela; Costa,Elaine; Ribeiro,Marisa; Godoy,Daniela Tupy de; Ferreira,Antonio G.P.; Tanuri,Amilcar; Brindeiro,Rodrigo; Alvarez,Patrícia. |
| Abstract Hepatitis C virus (HCV) infection is a worldwide health problem. Nowadays, direct-acting antiviral agents (DAAs) are the main treatment for HCV; however, the high level of virus variability leads to the development of resistance-associated variants (RAVs). Thus, assessing RAVs in infected patients is important for monitoring treatment efficacy. The aim of our study was to investigate the presence of naturally occurring resistance mutations in HCV NS3 and NS5 regions in treatment-naïve patients. Ninety-six anti-HCV positive serum samples from blood donors at the Center of Hematology and Hemotherapy of Santa Catarina State (HEMOSC) were collected retrospectively in 2013 and evaluated in this study. HCV 1a (37.9%), 1b (25.3%), and 3a (36.8%) subtypes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Direct-acting antivirals; Resistance-associated substitutions; Blood donors; NS3; NS5b. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100107 |
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| Rosini,Nilton; Mousse,Donaida; Spada,Celso; Treitinger,Arício. |
| The prevalence of infection by the hepatitis B (HBV) and C (HCV) viruses varies among geographical regions. We evaluated 263,795 blood donor samples collected from 1999-2001 in various cities in the state of Santa Catarina to determine the prevalence of HbsAg, anti-HBc and anti-HCV markers. The markers were analyzed by immunoenzymatic tests, as determined by the Ministry of Health, and the data were obtained from blood banks and from ANVISA (the Brazilian National Agency for Sanitary Vigilance). There was a significant reduction in the mean frequency of HbsAg and anti-HBc during the study period, from 0.98% to 0.64% and from 8.83% to 5.35%, respectively, though they varied considerably among the different regions. There was also a decrease in the mean... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood donors; Hepatitis B; Hepatitis C; HbsAg; Anti-HBc; Anti-HCV. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000400006 |
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| Langhi Junior,Dante M.; Bordin,José O.; Castelo,Adauto; Walter,Stephen D.; Moraes-Souza,Hélio; Stumpf,Robert J.. |
| The main strategy to prevent transfusion-associated Chagas' disease is the identification of T. Cruzi-infected blood donors by serological screening tests, however there is no perfect serological gold standard. We evaluated an enzyme immunoassay (EIA), an indirect hemaglutination (IHA), and an indirect immunofluorescence (IIF) test for detecting T. Cruzi antibodies in Brazilian blood donors. The results were submitted to latent class analysis, and a radioimmunopreciptation (RIPA) test was performed on repeatedly positive samples. Among 1951 donors, 11 (0.56%) were positive by EIA, 6 (0.31%) by IHA and 16 (0.82%) by IIF. Six samples were positive with all tests, while 4 reacted with EIA and IIF. The RIPA was positive in 6 (75.0%), 7 (66.6%), and 4 (54.0%)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chaga's disease; Blood donors; Diagnostic test; Latent class analysis. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000400005 |
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| Registros recuperados: 18 | |
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