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| Registros recuperados: 22 | |
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| VERARDO, L. L.; STAFUZZA, N. B.; MUNARI, D. P.; ZERLOTINI NETO, A.; CHUD, T. C. S.; GARRICK, D. J.; COLE, J. B.; PANETTO, J. C. do C.; MACHADO, M. A.; MARTINS, M. F.; SILVA, M. V. G. B.. |
| The aim of this study was to analyze whole-genome re-sequencing data focusing on SNVs and InDels identified in Gir, Girolando and Holstein cattle breeds related to RFI. Thus, genes showing SNVs/InDels in TF binding sites (5' UTR variants) were used to search for TF related to feed intake and to generate a gene-TF network for RFI across two purebred and one admixed dairy cattle breeds. Moreover, we were able to perform a comparative analysis accessing similarities and dissimilarities at the genomic level across these breeds. |
| Tipo: Artigo em anais de congresso (ALICE) |
Palavras-chave: Sequência de dados; Single Nucleotide Variations (SNVs); Insertions/deletions (InDels); Whole-genome; Whole-genome re-sequencing data; Gado de Corte; Bos Indicus; Variação Genética; Cattle breeds. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105518 |
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| OLIVEIRA, P. P. A.; TIZIOTO, P. C.; MALAGO JUNIOR, W.; NASCIMENTO, M. L. do; CESAR, A. S. M.; DINIZ, W. J. da S.; SOUZA, M. M. de; LANNA, D. P. D.; TULLIO, R. R.; MOURAO, G. B.; MUDADU, M. de A.; COUTINHO, L. L.; REGITANO, L. C. de A.. |
| Feed efficiency and carcass characteristics are late-measured traits. The detection of molecular markers associated with them can help breeding programs to select animals early in life, and to predict breeding values with high accuracy. The objective of this study was to identify polymorphisms in the functional and positional candidate gene NEUROD1 (neurogenic differentiation 1), and investigate their associations with production traits in reference families of Nelore cattle. A total of 585 steers were used, from 34 sires chosen to represent the variability of this breed. By sequencing 14 animals with extreme residual feed intake (RFI) values, seven single nucleotide polymorphisms (SNPs) in NEUROD1 were identified. The investigation of marker effects on... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Candidate gene; Feed efficiency; Bos Indicus; Body composition. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053741 |
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| SOUZA, M. M. de; ZERLOTINI NETO, A.; ROCHA, M. I. P.; BRUSCADIN, J. J.; DINIZ, W. J. da S.; CARDOSO, T. F.; CESAR, A. S. M.; AFONSO, J.; ANDRADE, B. G. N.; MUDADU, M. de A.; MOKRY, F. B.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; NICIURA, S. C. M.; COUTINHO, L. L.; REGITANO, L. C. de A.. |
| Differences between the expression of the two alleles of a gene are known as allele-specific expression (ASE), a common event in the transcriptome of mammals. Despite ASE being a source of phenotypic variation, its occurrence and effects on genetic prediction of economically relevant traits are still unexplored in bovines. Furthermore, as ASE events are likely driven by cis-regulatory mutations, scanning them throughout the bovine genome represents a significant step to elucidate the mechanisms underlying gene expression regulation. To address this question in a Bos indicus population, we built the ASE profile of the skeletal muscle tissue of 190 Nelore steers, using RNA sequencing data and SNPs genotypes from the Illumina BovineHD BeadChip (770 K bp).... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Expressão gênica; Regulação da expressão gênica; Allelic expression; Allele-specific expression; Bos Indicus; Gene expression regulation. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125395 |
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| PERIPOLLI, E.; STAFUZZA, N. B.; MUNARI, D. P.; LIMA, A. L. F.; IRGANG, R.; MACHADO, M. A.; PANETTO, J. C. do C.; VENTURA, R. V.; BALDI, F.; SILVA, M. V. G. B.. |
| Abstract BACKGROUND: Runs of homozygosity (ROH) are continuous homozygous segments of the DNA sequence. They have been applied to quantify individual autozygosity and used as a potential inbreeding measure in livestock species. The aim of the present study was (i) to investigate genome-wide autozygosity to identify and characterize ROH patterns in Gyr dairy cattle genome; (ii) identify ROH islands for gene content and enrichment in segments shared by more than 50% of the samples, and (iii) compare estimates of molecular inbreeding calculated from ROH (FROH), genomic relationship matrix approach (FGRM) and based on the observed versus expected number of homozygous genotypes (FHOM), and from pedigree-based coefficient (FPED). RESULTS: ROH were identified in... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Dairy traits; Inbreeding coefficients; ROH islands; Bos Indicus. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100275 |
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| OLIVEIRA, P. S. N. de; COUTINHO, L. L.; CESAR, A. S. M.; DINIZ, W. J. da S.; SOUZA, M. de S.; ANDRADE, B. G.; KOLTES, J. E.; MOURÃO, G. B.; ZERLOTINI NETO, A.; REECY, J. M.; REGITANO, L. C. de A.. |
| Fatty acid (FA) content affects the sensorial and nutritional value of meat and plays a significant role in biological processes such as adipogenesis and immune response. It is well known that, in beef, the main FAs associated with these biological processes are oleic acid (C18:1 cis9, OA) and conjugated linoleic acid (CLA-c9t11), which may have beneficial effects on metabolic diseases such as type 2 diabetes and obesity. Here, we performed differential expression and co-expression analyses, weighted gene co-expression network analysis (WGCNA) and partial correlation with information theory (PCIT), to uncover the complex interactions between miRNAs and mRNAs expressed in skeletal muscle associated with FA content. miRNA and mRNA expression data were... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Ácido linoleico conjugado; Ácido oleico; Ácidos graxos; Genômica; Redes de co-expressão; Integrative genomics; MRNA; MiRNA; Bos Indicus; Gado de Corte; Beef cattle; Conjugated linoleic acid; Oleic acid. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110820 |
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| ANDRADE, J. P. N.; GOMEZ-LÉON, V. E.; ANDRADE, F. S.; CARVALHO, B. P.; LACOUTH, K. L.; GARCIA, F. Z.; JACOB, J. C. F.; SALES, J. N. S.; WILTBANK, M. C.; MELLO, M. R. B.. |
| The aim was to develop a program for resynchronization of ovulation (ReBreed21) that allowed reinsemination of non-pregnant Bos indicus heifers every 21 d using timed AI (TAI) without the need for detection of estrus. The Rebreed21 program begins 12 d after previous TAI (Day 0) by inserting an intravaginal P4 implant (Day 12) that is removed 7 d later (Day 19) combined with treatment with 300 IU of eCG. On Day 21, early pregnancy diagnosis (Doppler PD) is performed based on CL vascularity. Nonpregnant (NP) heifers immediately received AI combined with 100 mg of GnRH. The program is replicated 12 d after second TAI to produce a breeding season (BS) of 42 d with 3 potential TAIs. Two ex periments were conducted as a proof of concept for this rapid rebreeding... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: ReBreed21; Ressincronização; Resynchronization; Razas zebú; Vaquilla; Cruce de animales; Métodos de mejoramiento genético; Inseminación artificial; Ovulación; Reprodução Animal; Gado Zebu; Bos Indicus; Gado Nelore; Novilho; Inseminação Artificial; Ovulação; Zebu breeds; Nellore; Heifers; Animal breeding; Breeding methods; Artificial insemination; Ovulation. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124659 |
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| CESAR, A. S. M.; REGITANO, L. C. de A.; POLETI, M. D.; ANDRADE, S. C. da S.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; FELICIO, A. M.; NASCIMENTO, M. L. do; CHAVES, A. S.; LANNA, D. P. D.; TULLIO, R. R.; NASSU, R. T.; KOLTES, J. E.; WATERS, E. F.; MOURAO, G. B.; ZERLOTINI NETO, A.; REECY, J. M.; COUTINHO, L. L.. |
| bitstream/item/153206/1/artigo5-Cesar-BMCGenomics-2016.pdf |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Bos Indicus; Human health; Lipids. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1060542 |
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| AFONSO, J.; FORTES, M. R. S.; REVERTER, A.; DINIZ, W. J. da S.; CESAR, A. S. M.; LIMA, A. O. de; PETRINI, J.; SOUZA, M. de S.; COUTINHO, L. L.; MOURÃO, G. B.; ZERLOTINI NETO, A.; GROMBONI, C. F.; NOGUEIRA, A. R. A.; REGITANO, L. C. de A.. |
| Mineral contents in bovine muscle can affect meat quality, growth, health, and reproductive traits. To better understand the genetic basis of this phenotype in Nelore (Bos indicus) cattle, we analysed genome-wide mRNA and miRNA expression data from 114 muscle samples. The analysis implemented a new application for two complementary algorithms: the partial correlation and information theory (PCIT) and the regulatory impact factor (RIF), in which we included the estimated genomic breeding values (GEBVs) for the phenotypes additionally to the expression levels, originally proposed for these methods. We used PCIT to determine putative regulatory relationships based on significant associations between gene expression and GEBVs for each mineral amount. Then, RIF... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Expressão gênica; Bos Indicus; Gado Nelore; Cattle; Gene expression. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124802 |
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| STAFUZZA, N. B.; SILVA, R. M. de O.; PERIPOLLI, E.; BEZERRA, L. A. F.; LOBO, R. B.; MAGNABOSCO, C. de U.; DI CROCE, F.; OSTERSTOCK, J.; MUNARI, D. P.; LOURENCO, D. A. L.; BALDI, F.. |
| Abstract The causal mutation for polledness in Nelore (Bos taurus indicus) breed seems to have appeared first in Brazil in 1957. The expression of the polled trait is known to be ruled by a few groups of alleles in taurine breeds; however, the genetic basis of this trait in indicine cattle is still unclear. The aim of this study was to identify genomic regions associated with the hornless trait in a commercial Nelore population. A total of 107,294 animals had phenotypes recorded and 2,238 were genotyped/imputed for 777k SNP. The weighted single-step approach for genome-wide association study (WssGWAS) was used to estimate the SNP effects and variances accounted for by 1 Mb sliding SNP windows. A centromeric region of chromosome 1 with 3.11 Mb size (BTA1:... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Gado Nelore; Mutação; Bos Indicus. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1101347 |
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| CARVALHO, M. E.; ELER, J. P.; BONIN, M. N.; REZENDE, F. M.; BIASE, F. H.; MEIRELLES, F. V.; REGITANO, L. C. de A.; COUTINHO, L. L.; BALIEIRO, J. C. C.; FERRAZ, J. B. S.. |
| The objectives of this study were to characterize the allelic and genotypic frequencies of polymorphisms in the μ-calpain and calpastatin genes, and to assess their association with meat tenderness and animal growth in Nellore cattle. We evaluated 605 Nellore animals at 24 months of age, on average, at slaughter. The polymorphisms were determined for the molecular markers CAPN316, CAPN530, CAPN4751, CAPN4753, and UOGACAST1. Analyses of meat tenderness at 7, 14, and 21 days of maturation were performed in samples of longissimus thoracis obtained between the 12th and 13th rib and sheared using a Warner Bratzler Shear Force. Significant effects were observed for meat tenderness at days 7, 14, and 21 of maturation for the marker CAPN4751, at day 21... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Nellore cattle; Bos Indicus; Polymorphism. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074518 |
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| COUTINHO, L. L.; MOROSINI, N. S.; CESAR, A. S. M.; POLETI, M. D.; REGITANO, L. C. de A.; MARGARIDO, G. R. A.. |
| In eukaryotes, DNA is organized along with histones in nucleoprotein complexes known as chromatin, which has nucleosomes as their fundamental unit. Chromatin Downloaded from https://academic.oup.com/jas/article-abstract/96/suppl_3/233/5235047 by Macquarie University user on 13 February 2019 234 J. Anim. Sci Vol. 96, Suppl. S3 exists in two forms: euchromatin, corresponding to a lightly condensed structure and an easily transcribed region, and heterochromatin, a highly condensed and transcriptionally silent region. |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: ATAC-seq; Bos Indicus; Gene expression; Skeletal muscle. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1106194 |
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| PAZ, C. C. P. de; FREITAS, A. R.; PACKER, I. U.; TAMBASCO-TALHARI, D.; REGITANO, L. C. de A.; ALENCAR, M. M. de. |
| Registros de pesos ao nascimento, ao desmame e mensais dos 8 aos 19 meses de idade referentes à animais dos grupos genéticos: ½Canchim-Nelore (CN), ½Angus-Nelore (AN) e ½Simental-Nelore (SN), pertencentes à Embrapa Pecuária Sudeste, São Carlos, SP, foram analisados pela técnica dos modelos não lineares incluindo, no modelo Logístico, os efeitos fixos de grupo contemporâneo e das classes de genótipos dos genes da kappacaseína-HinfI (CSN3): AA e AB, do hormônio do crescimento-AluI (GH): LL e LV e da b-lactoglobulina-HaeIII (LGB): AA, AB e BB, com o objetivo de verificar a influência destes genes sobre a curva de crescimento destes animais. Os resultados sugerem que, os parâmetros A e k, da função Logística utilizada para descrever o crescimento dos grupos... |
| Tipo: Artigo em anais de congresso (ALICE) |
Palavras-chave: Bos Taurus; Bos Indicus; Desemvolvimento ponderal; Marcadores genéticos; Modelo logístico. |
| Ano: 2003 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/46485 |
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| KAPPELER, B. I. G.; REGITANO, L. C. de A.; POLETI, M. D.; CESAR, A. S. M.; MOREIRA, G. C. M.; GASPARIN, G.; COUTINHO, L. L.. |
| MicroRNAs (miRNAs) are small noncoding RNAs of approximately 22 nucleotides, highly conserved among species, which modulate gene expression by cleaving messenger RNA target or inhibiting translation. MiRNAs are involved in the regulation of many processes including cell proliferation, differentiation, neurogenesis, angiogenesis, and apoptosis. Beef tenderness is an organoleptic characteristic of great influence in the acceptance of meat by consumers. Previous studies have shown that collagen level, marbling, apoptosis and proteolysis are among the many factors that affect beef tenderness. Considering that miRNAs can modulate gene expression, this study was designed to identify differentially expressed miRNAs that could be modulating biological processes... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Qualidade da carne; Bta-miR; Shear force; Gado de Corte; Carne; Bos Indicus; Beef cattle; Beef; Beef quality; MicroRNA. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1106070 |
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| DEFANT, H.; MEIRA, A. N.; COUTINHO, L. L.; REGITANO, L. C. de A.; POLETI, M. D.; MOREIRA, G. C. M.; PADUAN, M.; MARIANI, P.; ZERLOTINI NETO, A.; MOURÃO, G. B.; CESAR, A. S. M.. |
| ABSTRACT. Genetic variations in genes involved in lipid storage, which also may interfere with important phenotypic traits such as intramuscular fat deposition in cattle, can represent useful markers in marker assisted selection programs. An important candidate gene is perilipin 2 (PLIN2) that plays a role in the capture of long chain fatty acids and in the formation and stabilization of lipid droplets in different tissues including muscle. As reported by our group, PLIN2 expression level was associated with trans-eQTL (expression quantitative loci distant of gene associated) at chr3: 87, 253, 086 in Nellore cattle, where animals with different genotypes showed different expression level of PLIN2 among the 193 animals and the co-expression analysis... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Perilipin 2; PLIN2; Polimorfismo de nucleotídeo único; SNP; Processos biológicos; Gordura intramuscular; Gota lipídica; Expressão gênica; Biological process; Lipid droplet; Variants; Gado; Bos Indicus; Nellore; Cattle; Single nucleotide polymorphism; Gene expression; Intramuscular fat. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1119464 |
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| LIMA, A. O. de; KOLTES, J. E.; DINIZ, W. J. S.; OLIVEIRA, P. S. N. de; CESAR, A. S. M.; TIZIOTO, P. L.; AFONSO, J.; SOUZA, M. de S.; PETRINI, J.; ROCHA, M. I. P.; CARDOSO, T. F.; ZERLOTINI NETO, A.; COUTINHO, L. L.; MOURÃO, G. B.; REGITANO, L. C. de A.. |
| Feed efficiency helps to reduce environmental impacts from livestock production, improving beef cattle profitability. We identified potential biomarkers (hub genes) for feed efficiency, by applying co-expression analysis in Longissimus thoracis RNA-Seq data from 180 Nelore steers. Six co-expression modules were associated with six feed efficiency-related traits (p-value ≤ 0.05). Within these modules, 391 hub genes were enriched for pathways as protein synthesis, muscle growth, and immune response. Trait-associated transcription factors (TFs) ELF1, ELK3, ETS1, FLI1, and TCF4, were identified with binding sites in at least one hub gene. Gene expression of CCDC80, FBLN5, SERPINF1, and OGN was associated with multiple feed efficiency-related traits... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Longissimus thoracis; Biomarcadores; Expressão gênica; Feed efficiency; Hub genes; Systems biology; WGCNA; Bos Indicus; Gene expression. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124356 |
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| KASARAPU, P.; PORTO NETO, L. R.; FORTES, M. R. S.; LEHNERT, S. A.; MUDADU, M. de A.; COUTINHO, L. L.; REGITANO, L. C. de A.; GEORGE, A.; REVERTER, A.. |
| Numerical approaches to high-density single nucleotide polymorphism (SNP) data are often employed independently to address individual questions. We linked independent approaches in a bioinformatics pipeline for further insight. The pipeline driven by heterozygosity and Hardy-Weinberg equilibrium (HWE) analyses was applied to characterize Bos taurus and Bos indicus ancestry. We infer a gene co-heterozygosity network that regulates bovine fertility, from data on 18,363 cattle with genotypes for 729,068 SNP. Hierarchical clustering separated populations according to Bos taurus and Bos indicus ancestry. The weights of the first principal component were subjected to Normal mixture modelling allowing the estimation of a gene?s contribution to the Bos taurus-Bos... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Hardy-Weinberg equilibrium; Bos Indicus; Bos Taurus. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074938 |
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| Registros recuperados: 22 | |
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