SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

A single nucleotide polymorphism in NEUROD1 is associated with production traits in nelore beef cattle.

VERARDO, L. L.; STAFUZZA, N. B.; MUNARI, D. P.; ZERLOTINI NETO, A.; CHUD, T. C. S.; GARRICK, D. J.; COLE, J. B.; PANETTO, J. C. do C.; MACHADO, M. A.; MARTINS, M. F.; SILVA, M. V. G. B..

The aim of this study was to analyze whole-genome re-sequencing data focusing on SNVs and InDels identified in Gir, Girolando and Holstein cattle breeds related to RFI. Thus, genes showing SNVs/InDels in TF binding sites (5' UTR variants) were used to search for TF related to feed intake and to generate a gene-TF network for RFI across two purebred and one admixed dairy cattle breeds. Moreover, we were able to perform a comparative analysis accessing similarities and dissimilarities at the genomic level across these breeds.

Tipo: Artigo em anais de congresso (ALICE)

Palavras-chave: Sequência de dados; Single Nucleotide Variations (SNVs); Insertions/deletions (InDels); Whole-genome; Whole-genome re-sequencing data; Gado de Corte; Bos Indicus; Variação Genética; Cattle breeds.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105518

OLIVEIRA, P. P. A.; TIZIOTO, P. C.; MALAGO JUNIOR, W.; NASCIMENTO, M. L. do; CESAR, A. S. M.; DINIZ, W. J. da S.; SOUZA, M. M. de; LANNA, D. P. D.; TULLIO, R. R.; MOURAO, G. B.; MUDADU, M. de A.; COUTINHO, L. L.; REGITANO, L. C. de A..

Feed efficiency and carcass characteristics are late-measured traits. The detection of molecular markers associated with them can help breeding programs to select animals early in life, and to predict breeding values with high accuracy. The objective of this study was to identify polymorphisms in the functional and positional candidate gene NEUROD1 (neurogenic differentiation 1), and investigate their associations with production traits in reference families of Nelore cattle. A total of 585 steers were used, from 34 sires chosen to represent the variability of this breed. By sequencing 14 animals with extreme residual feed intake (RFI) values, seven single nucleotide polymorphisms (SNPs) in NEUROD1 were identified. The investigation of marker effects on...

Allele-specific expression is widespread in Bos indicus muscle and affects meat quality candidate genes.

Palavras-chave: Candidate gene; Feed efficiency; Bos Indicus; Body composition.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053741

SOUZA, M. M. de; ZERLOTINI NETO, A.; ROCHA, M. I. P.; BRUSCADIN, J. J.; DINIZ, W. J. da S.; CARDOSO, T. F.; CESAR, A. S. M.; AFONSO, J.; ANDRADE, B. G. N.; MUDADU, M. de A.; MOKRY, F. B.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; NICIURA, S. C. M.; COUTINHO, L. L.; REGITANO, L. C. de A..

Differences between the expression of the two alleles of a gene are known as allele-specific expression (ASE), a common event in the transcriptome of mammals. Despite ASE being a source of phenotypic variation, its occurrence and effects on genetic prediction of economically relevant traits are still unexplored in bovines. Furthermore, as ASE events are likely driven by cis-regulatory mutations, scanning them throughout the bovine genome represents a significant step to elucidate the mechanisms underlying gene expression regulation. To address this question in a Bos indicus population, we built the ASE profile of the skeletal muscle tissue of 190 Nelore steers, using RNA sequencing data and SNPs genotypes from the Illumina BovineHD BeadChip (770 K bp)....

Assessment of runs of homozygosity islands and estimates of genomic inbreeding in Gyr (Bos indicus) dairy cattle.

Palavras-chave: Expressão gênica; Regulação da expressão gênica; Allelic expression; Allele-specific expression; Bos Indicus; Gene expression regulation.

Ano: 2020

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125395

PERIPOLLI, E.; STAFUZZA, N. B.; MUNARI, D. P.; LIMA, A. L. F.; IRGANG, R.; MACHADO, M. A.; PANETTO, J. C. do C.; VENTURA, R. V.; BALDI, F.; SILVA, M. V. G. B..

Abstract BACKGROUND: Runs of homozygosity (ROH) are continuous homozygous segments of the DNA sequence. They have been applied to quantify individual autozygosity and used as a potential inbreeding measure in livestock species. The aim of the present study was (i) to investigate genome-wide autozygosity to identify and characterize ROH patterns in Gyr dairy cattle genome; (ii) identify ROH islands for gene content and enrichment in segments shared by more than 50% of the samples, and (iii) compare estimates of molecular inbreeding calculated from ROH (FROH), genomic relationship matrix approach (FGRM) and based on the observed versus expected number of homozygous genotypes (FHOM), and from pedigree-based coefficient (FPED). RESULTS: ROH were identified in...

Associação entre variantes cis regulatórias do gene KCNJ11e características econômicas em bovinos Nelore.

Palavras-chave: Dairy traits; Inbreeding coefficients; ROH islands; Bos Indicus.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100275

OLIVEIRA, K. S. de; LIMA, A. O. de; AFONSO, J.; CARDOSO, T. F.; BRUSCADIN, J. J.; REGITANO, L. C. de A..

Características de produção como eficiência alimentar e de qualidade da carne bovina são de suma importância para o agronegócio, agroindústria e consumidores. Assim, ferramentas biotecnológicas como a genômica podem adicionar informações para identificar potenciais biomarcadores com a finalidade de auxiliar os programas de melhoramento e seleção de bovinos de corte.

Associação entre variantes da região upstream do gene CTGF e medidas fenotípicas de eficiência alimentar em bovinos Nelore.

Palavras-chave: SNP; Expressão gênica; Sítio de ligação de fator de transcriçã0; Ilha CpG; Qualidade da carne; Bos Indicus.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115556

OLIVEIRA, K. S. DE; CARDOSO, T. F.; BRUSCADIN, J. J.; REGITANO, L. C. de A..

A eficiência alimentar bovina é uma característica de produção de suma importância para o agronegócio, agroindústria e meio ambiente, porém por possuir mensuração onerosa e tardia é de difícil integração a programas de melhoramento animal. Análises genômicas podem acrescentar informações para a identificação de potenciais biomarcadores, com a finalidade de auxiliar os programas de melhoramento, na seleção de bovinos Nelore mais eficientes quanto ao aproveitamento do alimento consumido. A partir dessas ferramentas, estudos prévios identificaram a expressão diferencial do gene connective tissue growth factor (CTGF) entre animais com fenótipos divergentes para eficiência alimentar, observando maiores níveis de expressão em animais ineficientes.

Co-expression networks reveal potential regulatory roles of miRNAs in fatty acid composition of nelore cattle.

Palavras-chave: SNP; Expressão gênica; Sítio de ligação de fator de transcrição; Bos Indicus; Fenótipo.

Ano: 2020

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131319

OLIVEIRA, P. S. N. de; COUTINHO, L. L.; CESAR, A. S. M.; DINIZ, W. J. da S.; SOUZA, M. de S.; ANDRADE, B. G.; KOLTES, J. E.; MOURÃO, G. B.; ZERLOTINI NETO, A.; REECY, J. M.; REGITANO, L. C. de A..

Fatty acid (FA) content affects the sensorial and nutritional value of meat and plays a significant role in biological processes such as adipogenesis and immune response. It is well known that, in beef, the main FAs associated with these biological processes are oleic acid (C18:1 cis9, OA) and conjugated linoleic acid (CLA-c9t11), which may have beneficial effects on metabolic diseases such as type 2 diabetes and obesity. Here, we performed differential expression and co-expression analyses, weighted gene co-expression network analysis (WGCNA) and partial correlation with information theory (PCIT), to uncover the complex interactions between miRNAs and mRNAs expressed in skeletal muscle associated with FA content. miRNA and mRNA expression data were...

Comparative gene frequences of nucleoside phosphorylase from cattle of Bos taurus and Bos indicus derivation in Brazil.

Palavras-chave: Ácido linoleico conjugado; Ácido oleico; Ácidos graxos; Genômica; Redes de co-expressão; Integrative genomics; MRNA; MiRNA; Bos Indicus; Gado de Corte; Beef cattle; Conjugated linoleic acid; Oleic acid.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110820

PANEPUCCI, L.; VICENTE, V.; ALENCAR, M. M. de; MORTARI, N..

Two nucleoside phosphorylase (NP) phenotypes were detected in 844 animals from four distinct genetic grroups of Bows indicus e Bos taurus.

Development of a novel 21-day reinsemination program, ReBreed21, in Bos indicus heifers.

Palavras-chave: Bos Indicus; Bos Taurus; Bovino; Gado Zebu; Gene.

Ano: 1990

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/42322

ANDRADE, J. P. N.; GOMEZ-LÉON, V. E.; ANDRADE, F. S.; CARVALHO, B. P.; LACOUTH, K. L.; GARCIA, F. Z.; JACOB, J. C. F.; SALES, J. N. S.; WILTBANK, M. C.; MELLO, M. R. B..

The aim was to develop a program for resynchronization of ovulation (ReBreed21) that allowed reinsemination of non-pregnant Bos indicus heifers every 21 d using timed AI (TAI) without the need for detection of estrus. The Rebreed21 program begins 12 d after previous TAI (Day 0) by inserting an intravaginal P4 implant (Day 12) that is removed 7 d later (Day 19) combined with treatment with 300 IU of eCG. On Day 21, early pregnancy diagnosis (Doppler PD) is performed based on CL vascularity. Nonpregnant (NP) heifers immediately received AI combined with 100 mg of GnRH. The program is replicated 12 d after second TAI to produce a breeding season (BS) of 42 d with 3 potential TAIs. Two ex periments were conducted as a proof of concept for this rapid rebreeding...

Differences in the skeletal muscle transcriptome profile associated with extreme values of fatty acids content.

Palavras-chave: ReBreed21; Ressincronização; Resynchronization; Razas zebú; Vaquilla; Cruce de animales; Métodos de mejoramiento genético; Inseminación artificial; Ovulación; Reprodução Animal; Gado Zebu; Bos Indicus; Gado Nelore; Novilho; Inseminação Artificial; Ovulação; Zebu breeds; Nellore; Heifers; Animal breeding; Breeding methods; Artificial insemination; Ovulation.

Ano: 2020

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124659

CESAR, A. S. M.; REGITANO, L. C. de A.; POLETI, M. D.; ANDRADE, S. C. da S.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; FELICIO, A. M.; NASCIMENTO, M. L. do; CHAVES, A. S.; LANNA, D. P. D.; TULLIO, R. R.; NASSU, R. T.; KOLTES, J. E.; WATERS, E. F.; MOURAO, G. B.; ZERLOTINI NETO, A.; REECY, J. M.; COUTINHO, L. L..

bitstream/item/153206/1/artigo5-Cesar-BMCGenomics-2016.pdf

Economic efficiency of Rhipicephalus microplus control and effect on beef cattle performance in the Brazilian Cerrado.

Palavras-chave: Bos Indicus; Human health; Lipids.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1060542

CALVANO, M. P. C. A.; BRUMATTI, R. C.; GARCIA, M. V.; BARROS, J. C.; ANDREOTTI, R..

The cattle tick Rhipicephalus microplus causes significant economic losses to cattle production systems and is a main barrier to the introduction of Bos taurus breeds and their crosses in Brazil. These breeds have the genetic potential to generate animals that are more productive, but they are also more susceptible to R. microplus. One of the alternatives for conventional tick control is the use of strategic control, aiming at delaying or even preventing the development of its resistance to acaricides. The present study aimed to evaluate the economic losses caused by tick infestation on the productive performance of two breeds of beef cattle and to evaluate the economic efficiency of tick strategic control and its impacts on beef cattle production systems....

Funções de covariâncias sobre polinômios B-splines para modelagem do crescimento de bovinos de corte: revisão.

Palavras-chave: Cost; Economic loss; Infestation; Strategic control; Bos Indicus; Bos Taurus.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117934

CAVALCANTE, D. H.; EVANGELISTA, A. F.; CAMPELO, J. E. G.; AZEVEDO, D. M. M. R.; SOUSA JÚNIOR, S. C..

O objetivo é abordar assuntos sobre polinômios B-splines utilizados em funções de covariância por meio de modelos de regressão aleatória com a finalidade de predizer o potencial genético em bovinos de corte em características de crescimento. Nos modelos de regressão aleatória é possível decompor a trajetória da curva de crescimento por meio das mudanças nas covariâncias dos efeitos aleatórios. O tipo e a ordem de ajuste dos polinômios utilizados para modelagem do crescimento podem influenciar os resultados das avaliações genéticas, portanto, é necessário avaliar diferentes modelos para se auferir o mais harmônico.

Genetic regulators of mineral amount in Nelore cattle muscle predicted by a new co-expression and regulatory impact factor approach.

Palavras-chave: Avaliação genética; Parâmetros genéticos; Bos Indicus.

Ano: 2020

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124504

AFONSO, J.; FORTES, M. R. S.; REVERTER, A.; DINIZ, W. J. da S.; CESAR, A. S. M.; LIMA, A. O. de; PETRINI, J.; SOUZA, M. de S.; COUTINHO, L. L.; MOURÃO, G. B.; ZERLOTINI NETO, A.; GROMBONI, C. F.; NOGUEIRA, A. R. A.; REGITANO, L. C. de A..

Mineral contents in bovine muscle can affect meat quality, growth, health, and reproductive traits. To better understand the genetic basis of this phenotype in Nelore (Bos indicus) cattle, we analysed genome-wide mRNA and miRNA expression data from 114 muscle samples. The analysis implemented a new application for two complementary algorithms: the partial correlation and information theory (PCIT) and the regulatory impact factor (RIF), in which we included the estimated genomic breeding values (GEBVs) for the phenotypes additionally to the expression levels, originally proposed for these methods. We used PCIT to determine putative regulatory relationships based on significant associations between gene expression and GEBVs for each mineral amount. Then, RIF...

Genética aplicada para pequenos e médios produtores de gado de corte.

Palavras-chave: Expressão gênica; Bos Indicus; Gado Nelore; Cattle; Gene expression.

Ano: 2020

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124802

Infoteca-e

ROSA, A. do N.; MENEZES, G. R. de O.; EGITO, A. A. do; NOGUEIRA, E..

bitstream/item/221998/1/DOC-288-Final-em-Alta.pdf

Tipo: Documentos (INFOTECA-E)

Palavras-chave: Bos Indicus; Gado Zebu; Melhoramento Genético Animal; Pecuária; Sistema de Produção.

Ano: 2021

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1130820

Genome-wide association study of weight gain in feedlot Nellore cattle: comparison of single nucleotide polymorphism and haplotype blocks approaches.

SOMAVILLA, A. L.; MOTA, M. D. S.; ALENCAR, M. M. de; ROSA, A. do N.; COUTINHO, L. L.; TULLIO, R. R.; MUDADU, M. de A.; REGITANO, L. C. de A..

bitstream/item/86853/1/PROCI-2013.00067.pdf

Genome-wide association study provides insights into genes related with horn development in Nelore beef cattle.

Palavras-chave: Growth; QTL; SNP; Bos Indicus; Beef cattle.

Ano: 2013

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/963137

STAFUZZA, N. B.; SILVA, R. M. de O.; PERIPOLLI, E.; BEZERRA, L. A. F.; LOBO, R. B.; MAGNABOSCO, C. de U.; DI CROCE, F.; OSTERSTOCK, J.; MUNARI, D. P.; LOURENCO, D. A. L.; BALDI, F..

Abstract The causal mutation for polledness in Nelore (Bos taurus indicus) breed seems to have appeared first in Brazil in 1957. The expression of the polled trait is known to be ruled by a few groups of alleles in taurine breeds; however, the genetic basis of this trait in indicine cattle is still unclear. The aim of this study was to identify genomic regions associated with the hornless trait in a commercial Nelore population. A total of 107,294 animals had phenotypes recorded and 2,238 were genotyped/imputed for 777k SNP. The weighted single-step approach for genome-wide association study (WssGWAS) was used to estimate the SNP effects and variances accounted for by 1 Mb sliding SNP windows. A centromeric region of chromosome 1 with 3.11 Mb size (BTA1:...

Genotypic and allelic frequencies of gene polymorphisms associated with meat tenderness in Nellore beef cattle.

Palavras-chave: Gado Nelore; Mutação; Bos Indicus.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1101347

CARVALHO, M. E.; ELER, J. P.; BONIN, M. N.; REZENDE, F. M.; BIASE, F. H.; MEIRELLES, F. V.; REGITANO, L. C. de A.; COUTINHO, L. L.; BALIEIRO, J. C. C.; FERRAZ, J. B. S..

The objectives of this study were to characterize the allelic and genotypic frequencies of polymorphisms in the μ-calpain and calpastatin genes, and to assess their association with meat tenderness and animal growth in Nellore cattle. We evaluated 605 Nellore animals at 24 months of age, on average, at slaughter. The polymorphisms were determined for the molecular markers CAPN316, CAPN530, CAPN4751, CAPN4753, and UOGACAST1. Analyses of meat tenderness at 7, 14, and 21 days of maturation were performed in samples of longissimus thoracis obtained between the 12th and 13th rib and sheared using a Warner Bratzler Shear Force. Significant effects were observed for meat tenderness at days 7, 14, and 21 of maturation for the marker CAPN4751, at day 21...

Identification and characterization of euchromatic regions associated with gene expression and intramuscular fat in Nelore cattle.

Palavras-chave: Nellore cattle; Bos Indicus; Polymorphism.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074518

COUTINHO, L. L.; MOROSINI, N. S.; CESAR, A. S. M.; POLETI, M. D.; REGITANO, L. C. de A.; MARGARIDO, G. R. A..

In eukaryotes, DNA is organized along with histones in nucleoprotein complexes known as chromatin, which has nucleosomes as their fundamental unit. Chromatin Downloaded from https://academic.oup.com/jas/article-abstract/96/suppl_3/233/5235047 by Macquarie University user on 13 February 2019 234 J. Anim. Sci Vol. 96, Suppl. S3 exists in two forms: euchromatin, corresponding to a lightly condensed structure and an easily transcribed region, and heterochromatin, a highly condensed and transcriptionally silent region.

Indução de ovulação em novilhas pré-púberes da raça Gir.

Palavras-chave: ATAC-seq; Bos Indicus; Gene expression; Skeletal muscle.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1106194

ROSA, P. M. da S.; BARROS, B. A. F. de; PASOLINI, R.; CAMARGO, A. J. dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S..

2016

Influência dos polimorfismos genéticos sobre os parâmetros da curva de crescimento em bovinos de corte.

Palavras-chave: Bos Indicus; Dinâmica folicular; Progesterona; Puberdade; Ultrassonografia.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1062182