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A gravity model approach to forecasting tuberculosis transmission in cattle AgEcon
Xie, Fang; Horan, Richard D.; Wolf, Christopher A..
Bovine tuberculosis (bTB) in cattle has caused significant economic losses to livestock producers and has proven difficult to eradicate. It is suspected that cattle movement across different farms and regions is one of the key factors of bTB transmission in the United States. Prior attempts to model the epidemiology of bTB infection within cattle to predict disease transmission have not adequately captured the behavioral aspects of trade. A better understanding of livestock trade patterns would help in predicting disease transmission and the associated economic effects. In this paper, we develop a gravity model of livestock trade and link it to an epidemiological model of bTB transmission, with the goal being that this information could lead to improved...
Tipo: Conference Paper or Presentation Palavras-chave: Bovine tuberculosis; Gravity model; Disease management; Resource /Energy Economics and Policy.
Ano: 2009 URL: http://purl.umn.edu/49382
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Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR Ciência Rural
Moura,André; Hodon,Mikael Arrais; Soares Filho,Paulo Martins; Issa,Marina de Azevedo; Oliveira,Ana Paula Ferreira de; Fonseca Júnior,Antônio Augusto.
ABSTRACT: Bovine tuberculosis is an infectious disease with a high impact on the cattle industry, particularly in developing countries. PCR is a very sensitive method for detection of infectious agents, but the sensitivity of molecular diagnosis is largely dependent on the efficiency of the DNA extraction methods. The objective of this study was to evaluate DNA extraction methods for direct detection of Mycobacterium bovis in bovine tissue. Nine commercial kits for DNA extraction were evaluated when combined with two real time PCRs. The DNeasy Blood & Tissue Kit from QIAGEN showed better performance and sensitivity followed by the DNA Mini Kit RBC and FTA Elute Micro Card. Results suggested that, even when the analytical sensitivity of the qPCR is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine tuberculosis; DNA extraction; Real time PCR.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701223
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Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR BJM
Figueiredo,Eduardo Eustáquio de Souza; Carvalho,Ricardo Cezar Tavares; Silvestre,Flávia Galindo; Lilenbaum,Walter; Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi.
Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Nasal swab; Bovine tuberculosis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200020
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ELISA using a recombinant chimera of ESAT-6/MPB70/MPB83 for Mycobacterium bovis diagnosis in naturally infected cattle. Repositório Alice
SOUZA, I. I. F. de; RODRIGUES, R. de A.; JORGE, K. S. G.; SILVA, M. R.; LILENBAUM, W.; VIDAL, C. E. S.; ETGES, R. N.; KOSTOVIC, M.; ARAUJO, F. R..
Bovine tuberculosis (bTB) control programs generally rely on intradermal tuberculin tests for the antemortem diagnosis of Mycobacterium bovis infection in cattle, but these tests detect only a portion of the infected animals. The aim of the present study was to evaluate the diagnostic coverage of a combination of the bTB antemortem techniques known as the comparative intradermal tuberculin test (CITT) and an ELISA based on a recombinant chimera of ESAT-6/MPB70/MPB83 as the antigen in cattle. The results were compared to postmortem findings based on M. bovis culturing and PCR. Paired comparisons of all data (n=92) demonstrated that ELISA and LST results compared to the culturing results did not present significant differences (P=0.27 on McNemar?s test and...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Comparative intradermal tuberculin test; Bovine tuberculosis; Mycobacterium bovis BCG.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117817
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Epidemiological Study of Mycobacterium bovis Infection in Buffalo and Cattle in Amazonas, Brazil. Repositório Alice
CARNEIRO, P. A. M.; TAKATANI, H.; PASQUATTI, T. N.; SILVA, C. B. D. G.; NORBY, B.; WILKINS, M. J.; ZUMÁRRAGA, M. J.; ARAUJO, F. R.; KANEENE, J. B..
Bovine Tuberculosis (BTB) is an endemic disease in about one hundred countries, affecting the economy causing a decrease in productivity, condemnation of meat, and damaging the credibility on international trade. Additionally, Mycobacterium bovis the major causative agent for BTB can also infect humans causing a variety of clinical presentations. The aim of this study was to determine BTB prevalence and the main risk factors for theMycobacterium bovis prevalence in cattle and buffalos in Amazonas State, Brazil. Tissue samples from 151 animals (45 buffalo and 106 cattle from five herds with buffalo only, 22 herds with cattle only, and 12 herds with buffalo and cattle) were obtained from slaughterhouses under State Veterinary Inspection. M. bovis were...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bovine tuberculosis; Mycobacterium bovis BCG; Cattle; Buffaloes; Epidemiology.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117948
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Epidemiology of bovine tuberculosis in Mexico, bordering the United States, at establishment of controlling strategies Arq. Bras. Med. Vet. Zootec.
Enríquez-Cruz,C.; Cruz-Hernández,N.I.; Zertuche-Rodríguez,J.L.; Uriegas-García,J.L.; Toscano-Ruiz,J.E.; Flores-Gutiérrez,G.H..
A case-control study was carried out for determining epidemiological patterns at the beginning of the Mexican campaign against bovine tuberculosis (BTB) in the State of Tamaulipas, Mexico, bordering the United States of America. From January 1995 to March 1998, 599 direct tissue smears of cattle from routine slaughterhouse inspection were analyzed for the presence of Mycobacterium bovis acid-fastness with Ziehl-Neelsen stain. BTB status was associated with year of diagnosis, age, breed, geographical origin of cattle, and type of slaughterhouse using the odds ratio (OR) (95% confidence level). The prevalence at slaughterhouse surveillance was 15.36%, which was considerably higher in 1995 (41.38%, OR=5.78, P<0.0001), in comparison with 8.09%, 14.42%, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine tuberculosis; United States/Mexico border; Epidemiologic control.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000500002
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Evaluation of the efficiency of nested q-PCR in the detection of Mycobacterium tuberculosis complex directly from tuberculosis-suspected lesions in post-mortem macroscopic inspections of bovine carcasses slaughtered in the state of Mato Grosso, Brazil. Repositório Alice
CARVALHO, R. C. T.; ARAUJO, F. R..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Mycobacterium tuberculosis complex; Mycobacterium bovis; Nested q-PCR; Post-mortem meat inspection; Bovine tuberculosis.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1017967
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Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil. Repositório Alice
SOUZA-FILHO, A. F. de; OSÓRIO, A. L. A. R.; JORGE, K. dos S. G.; ARAUJO, F. R.; VIDAL, C. E. S.; ARAÚJO, C. P.; ALBERTTI, L. A. G.; CAZOLA, D. de O.; FERREIRA NETO, J. S.; HEINEMANN, M. B..
Mycobacterium bovis is the agent of bovine tuberculosis, a disease endemic to all Brazilian states. Molecular typing techniques help to stratify and refine data, providing information that facilitates epidemiological research. In this study, MIRU-VNTR, targeting 24 loci, was employed to identify and characterize the genetic groups of M. bovis isolates obtained from an outbreak of bovine tuberculosis. Eighteen acid-fast bacilli isolates, obtained from bovine tissue samples, and reactive to the comparative cervical tuberculin test, were identified as species of the M. tuberculosis complex, and were genotyped by MIRU-VNTR with 24 primer pairs. Genotyping revealed three genetic profiles comprising one with 15 isolates (83.3%), one with two isolates (11.1%),...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Diversidade genética; Tuberculose bovina; MIRU-VNTR; Genetical diversity; Mycobacterium bovis; Bovine tuberculosis; Mycobacterium bovis.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1087984
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Identificação e genotipagem de Mycobacterium bovis em bovinos positivos no teste intradérmico para tuberculose em Mato Grosso do Sul1. Repositório Alice
CAZOLA, D de O.; JORGE, K. dos S. G.; ZUMÁRRAGA, M. J.; SOUZA-FILHO, A. F.; ARAUJO, F. R.; OSÓRIO, A. L. A. R..
Neste estudo, realizou-se genotipagem de isolados de Mycobacterium bovis, provenientes de amostras de tecidos de bovinos positivos no teste cervical comparativo (TCC) para tuberculose em Mato Grosso do Sul, por meio da técnica de spoligotyping.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Mycobacterium bovis; Molecular identification; Molecular epidemiology; Poligotyping; Bovine tuberculosis.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1015208
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Identification and classification of Mycobacterium by MALDI-TOF mass spectrometry. Repositório Alice
OLARTE, L. C.; DUARTE, L. de F. C.; LEGUIZAMON, G. O. de C.; ARAUJO, F. R.; VERBISCK, N. V..
bitstream/item/166582/1/Identification-and-classification-of-Mycobacterium.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Mycobacterium; MALDI-TOF mass spectrometry; Bovine tuberculosis; Species identification; Mycobacterium bovis; Mycobacterium smegmatis.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1079459
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Identification of Mycobacterium bovis antigens by analysis of bovine T-cell responses after infection with a virulent strain BJMBR
Alito,A.; McNair,J.; Girvin,R.M.; Zumarraga,M.; Bigi,F.; Pollock,J.M.; Cataldi,A..
Purification and characterization of individual antigenic proteins are essential for the understanding of the pathogenic mechanisms of mycobacteria and the immune response against them. In the present study, we used anion-exchange chromatography to fractionate cell extracts and culture supernatant proteins from Mycobacterium bovis to identify T-cell-stimulating antigens. These fractions were incubated with peripheral blood mononuclear cells (PBMC) from M. bovis-infected cattle in lymphoproliferation assays. This procedure does not denature proteins and permits the testing of mixtures of potential antigens that could be later identified. We characterized protein fractions with high stimulation indices from both culture supernatants and cell extracts....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine tuberculosis; Antigens; Mycobacterium bovis; Cellular immunity.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100011
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Identification of Mycobacterium bovis Isolates by a multiplex PCR BJM
Figueiredo,Eduardo Eustáquio de Souza; Silvestre,Flávia Galindo; Campos,Wilma Neres; Furlanetto,Leone Vinícius; Medeiros,Luciana; Lilenbaum,Walter; Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi.
Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Bovine tuberculosis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200004
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Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae. Repositório Alice
BACANELLI, G.; OLARTE, L. C.; SILVA, M. R.; RODRIGUES, R. A.; CARNEIRO, P. A. M.; KANEENE, J. B.; PASQUATTI, T. N.; TAKATANI, H.; ZUMÁRRAGA, M. J.; ETGES, R. N.; ARAUJO, F. R.; VERBISCK, N. V..
In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDITOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry; Bovine tuberculosis; Mycobacterium bovis BCG; Mycobacterium tuberculosis complex.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117863
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Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil. Repositório Alice
CARNEIRO, P. A. M.; PASQUATTI, T. N.; TAKATANI, H.; ZUMÁRRAGA, M. J.; MARFIL, M. J.; BARNARD, C.; FITZGERALD, S. D.; ABRAMOVITCH, R. B.; ARAUJO, F. R.; KANEEENE, J. B..
The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cattle and buffalo; Genotypes; Spoligotyping; Mycobacterium Bovis; Bovine tuberculosis; Mycobacterium bovis BCG.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117813
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Molecular typing of mycobacterium bovis from cattle reared in Midwest Brazil. Repositório Alice
CARVALHO, R. C. T.; VASCONCELLOS, S. E. G.; ISSA, M. de A.; SOARES FILHO, P. M.; MOTA, P. M. P. C.; ARAUJO, F. R.; CARVALHO, A. C. da S.; GOMES, H. M.; SUFFYS, P. N.; FIGUEIREDO, E. E. de S.; PASCHOALIN, V. M. F..
Mycobacterium bovis is the causative agent of bovine tuberculosis (BTB), the pathogen responsible for serious economic impact on the livestock sector. In order to obtain data on isolated M. bovis strains and assist in the control and eradication program for BTB, a cross sectional descriptive molecular epidemiology study in the Brazilian Midwest was conducted. Through spoligotyping and 24-loci MIRU-VNTR methods, 37 clinical isolates of M. bovis circulating in the region were analyzed, 10 isolated from the state of Mato Grosso, 12 from the state of Mato Grosso do Sul and 15 from the state of Goiás. The spoligotyping analysis identified 10 distinct M. bovis profiles (SB0121 n = 14, SB0295 n = 6, SB0140 n = 6, SB0881 n = 3, SB1144 n = 2, SB1145 n = 2, SB0134 n...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Mycobacterium bovis; Bovine tuberculosis; Molecular epidemiology; Cattle; Brazil.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1061179
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Molecular typing of Mycobacterium bovis isolated in the south of Brazil BJM
Ramos,Daniela Fernandes; Silva,Ana Bárbara Scholante; Fagundes,Michel Quevedo; von Groll,Andrea; Silva,Pedro Eduardo Almeida da; Dellagostin,Odir Antônio.
Bovine tuberculosis is a major infectious disease of the cattle. In this study, 85 M. bovis isolates from 162 lymph nodes, obtained from a herd of cattle on a farm in southern Brazil, were evaluated using spoligotyping and VNTR. The strains were grouped into five clusters and five orphans, showing a heterogenic genetic profile, what could represent diverse geographic origins of the introduced cows and/or the frequent movement of cattle between different properties.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine tuberculosis; Mycobacterium bovis; Genotyping.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200039
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Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex BJM
Spositto,F.L.E.; Campanerut,P.A.Z.; Ghiraldi,L.D.; Leite,C.Q.F.; Hirata,M.H.; Hirata,R.D.C.; Siqueira,V.L.D.; Cardoso,R. Fressatti.
We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine tuberculosis; Tuberculosis; PCR-multiplex; Diagnosis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300012
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Patterns and Processes of Mycobacterium bovis Evolution Revealed by Phylogenomic Analyses. Repositório Alice
PATANE, J. S. L.; MARTINS JUNIOR, J.; CASTELÃO, A. B.; NISHIBE, C.; MONTERA, L.; BIGI, F.; ZUMÁRRAGA, M. J.; CATALDI, A. A.; FONSECA JUNIOR, A.; ROXO, E.; OSÓRIO, A. L. A. R.; JORNGE, K. S.; THACKER, T. C.; ALMEIDA, N. F.; ARAUJO, F. R.; SETUBAL, J. C..
Mycobacterium bovis is an important animal pathogen worldwide that parasitizes wild and domesticated vertebrate livestock as well as humans. A comparison of the five M. bovis complete genomes from the United Kingdom, South Korea, Brazil, and the United States revealed four novel large-scale structural variations of at least 2,000 bp.Acomparative phylogenomic study including 2,483 core genes of 38 taxa from eight countries showed conflicting phylogenetic signal among sites. By minimizing this effect,we obtained a tree that better agreeswith sampling locality. Results supported a relatively basal position of African strains (all isolated from Homo sapiens), confirming that Africa was an important region for early diversification and that humans were one of...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Phylogenomics; Tuberculose bovina; Filogenômica; Família PE / PPE; Mycobacterium bovis; Epidemiologia; Mycobacterium bovis; Bovine tuberculosis.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1077365
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Population structure, genotypes and pathogenic phenotype of Mycobacterium bovis in Argentina (and Latin America). Repositório Alice
ZUMÁRRAGA, M. J.; ARAUJO, F. R..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bovine tuberculosis; Mycobacterium bovis; Spoligotyping; Clusters.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1012228
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Prevalence of bovine tuberculosis in the Aksu Region of Xinjiang, China, between 1985 and 2016 Arq. Bras. Med. Vet. Zootec.
Liu,Y.H.; Kang,Q.; Yang,B.; Li,F.; Li,X.Q.; Zhang,L.; Zhao,L..
ABSTRACT Prevalence of bovine tuberculosis infection in cattle in Aksu Prefecture determined by intradermal tuberculin skin test (TST), between 1985 and 2016. Cattle were analyzed according to region, feeding pattern, herds and age. A total of 890,009 cattle were tested, with overall bovine tuberculosis prevalence of 0.13% (1172/890009). Statistically significant difference was found in feeding pattern and herds. Prevalence in cows (0.19%, 615/327022) was higher than that in beeves (P< 0.01, OR= 1.903, 95% CI = 1.696 to 2.134). Significant difference (P< 0.01; OR= 2.238, 95%; CI= 1.937 to 2.585) was evident for rates for bovine tuberculosis in the peasant household (0.12%, 942/802343) and farm groups (0.26%, 230/87666). The overall prevalence of bTB...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prevalence; Bovine tuberculosis; Aksu prefecture.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200374
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