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Registros recuperados: 13
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Avaliação da longevidade espermática após o uso de diferentes métodos de seleção de espermatozóides ovinos congelados. Repositório Alice
SOUZA-FABJAN, J. M. G. de; OLIVARES, C. C. S.; VELTRI, A. B. P.; BALARO, M. F. A.; FREITAS, V. J. de F.; FONSECA, J. F. da; BRANDÃO, F. Z..
2015
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: CASA; Incubação espermática; Sperm incubation; Sperm longevity; Raça Santa Inês; Ovino; Carneiro; Reprodução animal; Espermatozóide; Sêmen; Sheep; Animal reproduction; Spermatozoa; Semen; Rams.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1019653
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Categorização computadorizada da motilidade de espermatozoides criopreservados de touros compostos após desafio térmico in vitro. Repositório Alice
BOTTA, D.; ARRUDA, R. P. de; CARVALHO, C. P. T. de; ROMANELLO, N.; PANTOJA, M. H. de A.; BARRETO, A. do N.; LEMES, A. P.; GIRO, A.; MOURA, A. B.; GARCIA, A. R..
As amostras de sêmen criopreservado de 5 touros foram descongelados e submetidos a desafio térmico usando incubação a 38,0°C (Tratamento 1) e 39,5°C (Tratamento 2). As amostras foram analisadas imediatamente após a descongelação (M0) e após 1 hora de incubação (M1). As seguintes variáveis foram determinadas: motilidade total, porcentagem de espermatozóides com movimento rápido, médio ou lento e porcentagem de espermatozóides estáticos. As análises foram feitas usando o Sistema Computadorizado de Análise de Sêmen (CASA). Os dados foram submetidos à análise de variância e os meios foram comparados utilizando o teste de Tukey (P <0,05). A incubação do sêmen coletado a 38,0°C não influenciou a motilidade total, e a porcentagem de espermatozoides com...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Canchim; CASA; Estresse térmico.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1079835
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Characterization of sperm motility in sea bass: the effect of heavy metals and physicochemical variables on sperm motility ArchiMer
Abascal, F; Cosson, J; Fauvel, Christian.
Computer assisted sperm analysis (CASA) was used to characterize the motility of sea bass Dicentrarchus labrax spermatozoa and to study the effect of several physicochemical variables and heavy metals on sperm swimming performance. Duration of sperm motility in sea bass was very short (< 50 s). During the first 20 s all the motility variables measured remained approximately constant, the velocity and linearity of the movement being maximum during this period, while both variables decreased sharply later. While slight variations in pH did not significantly modify sperm swimming performance, changes in osmolality affected all the measured motility variables. Two of the heavy metals tested, Cu2+ and Pb2+, did not affect sperm motility when the activating...
Tipo: Text Palavras-chave: Sperm; Heavy metals; Dicentrarchus labrax; CASA.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4765.pdf
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Comparison of different sperm selection techniques in ram frozen-thawed sperm. Repositório Alice
OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z..
Resumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Sperm preparation; Sperm capacitation; CASA; CTC; Plasma membrane integrity; Protocolo; Ovino; Espermatozóide; Sêmen; Reprodução animal; Sheep; Reproduction; Reproductive techniques; Spermatozoa; Semen; Treaties.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1070178
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Efeito da radiação vermelha de baixa intensidade sobre o sêmen canino criopreservado Arq. Bras. Med. Vet. Zootec.
Brito,M.F.; Carvalho,T.T.C.; Valle,G.R.; Ferreira,L.F.L.; Mambrini,J.V.M.; Henry,M.R.J.M.; Melo,M.I.V..
O objetivo geral deste trabalho foi analisar o efeito da radiação vermelha de baixa intensidade sobre alguns parâmetros cinéticos do espermatozoide canino criopreservado. Ejaculados de oito cães adultos foram centrifugados, rediluídos em meio tris-gema de ovo com 6% de glicerol, e, posteriormente, fracionados em: T1: incidência de radiação vermelha (660 NM) (Fisioled - Mmoptics - 100mW) por 60 segundos, antes do resfriamento e após a descongelação; T2: incidência somente antes do resfriamento; T3: incidência somente após a descongelação; e T4: sem incidência. Após a descongelação, as amostras foram submetidas ao TTR utilizando-se Sperm Class Analyzer(r). No TTR0, TTR60 e TTR90, não houve diferença entre as variáveis analisadas pelo CASA. Somente no TTR30...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cão; Criopreservação; Radiação vermelha; CASA.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000100062
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Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm ArchiMer
Suquet, Marc; Quere, Claudie; Mingant, Christian; Lebrun, Luc; Ratiskol, Dominique; Miner, Philippe; Cosson, Jacky.
Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (YAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, YAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed...
Tipo: Text Palavras-chave: Spermatozoa; CASA; Maturation; Sperm motility; Bivalve; Pecten maximus.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26852/24983.pdf
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Fresh, equilibrated and post-thaw sperm quality of Brycon orbignyanus (Valenciennes, 1850) and Prochilodus lineatus (Valenciennes, 1837) treated with either salmon GnRHa and domperidone or pituitary extract Neotropical Ichthyology
Viveiros,Ana T.M.; Gonçalves,Antonio C.S.; Nascimento,Ariane F.; Leal,Marcelo C..
The effects of reduced doses of Ovaprim™ (GnRHa + domperidone) on sperm release of Brycon orbignyanus and Prochilodus lineatus were evaluated. Furthermore, sperm quality was compared among fresh, equilibrated and post-thaw samples. Males received a single and reduced dose of Ovaprim™ (0.125 or 0.25 ml/kg); control males received pituitary extract (cPE; 3 mg/kg). Fresh sperm was evaluated for volume, concentration, seminal plasma osmolality and seminal plasma pH. Then sperm was diluted in a freezing medium, equilibrated for 15-20 min and frozen in nitrogen vapor vessel (dry-shipper). Sperm motility was analyzed during 60 s post-activation in fresh, equilibrated and post-thaw samples. Sperm quality of males treated with Ovaprim™ (both doses) were not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CASA; Characiformes; Cryopreservation; Ovaprim™; Sperm motility.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000100157
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Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm Neotropical Ichthyology
Viveiros,Ana T. M.; Taffarel,Tais R.; Leal,Marcelo C..
This study evaluates the effects of extender osmolality and composition on the cooling of Prochilodus lineatussperm. Sperm was diluted in six extenders: two compositions (powdered coconut water(tm) = ACP; Beltsville Thawing Solution(tm) = BTS) x three osmolalities (285, 325, and 365 mOsm/kg) plus an undiluted control, and stored at 6-8°C. Motility rate and velocities (curvilinear, straight line, and average path) were determined every other day. Osmolality did not affect the quality of cooled sperm, thus data were pooled. Motility was higher on d 0 compared to the other days and diluted samples (85-90%) yielded higher motility than control (75%). On d 2, motility was higher in BTS-diluted samples and control, but on d 4 and 6, control yielded the highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CASA; Cooling; Motility; Semen; Velocity.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000300643
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Qualidade do sêmen criopreservado e fertilidade de fêmeas bovinas em programas de IATF: dados preliminares. Repositório Alice
SILVA, C. S.; RODRIGUES, W. B.; POTIENS, J. R.; SILVA, J. C. B. da; ROSSIGNOLO, E. A. de A.; BARBOSA, F. B.; SILVA, E. V. da C. e.; NOGUEIRA, E..
O estudo foi conduzido com objetivo de correlacionar os distintos métodos de avaliações laboratorial da criopreservação espermática com a taxa de prenhez de vacas zebuínas submetidas a protocolos de IATF, através de análise de regressão logística múltipla entre prenhez e parâmetros avaliados individualmente. Foram realizadas 19 análises de rotina laboratoriais de aspectos físicos, funcionais e morfológicos por meio de meios subjetivos, CASA e citometria de fluxo em amostras de sêmen de 20 touros utilizados nas 1.542 vacas Nelore submetidas à IATF. Foi observado o efeito da VCL (velocidade curvilínea), velocidade retilínea (VSL), linearidade (LIN) e concentração espermática (mL) na probabilidade de prenhez de vacas Nelore submetido à IATF. Os outros...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: CASA; Citometria de fluxo; Vaca; Fisiologia Reprodutiva; Reprodução animal; Animal reproduction.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1077019
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Qualidade do sêmen e resultados em programas de IATF em bovinos. Repositório Alice
NOGUEIRA, E.; SILVA, C. S.; SILVA, J. C. B.; GARCIA, W. R.; ANACHE, N. A.; SILVA, K. C.; POTIENS, J. R.; SILVA, E. V. da C. e..
The ability to predict male fertility is highly desirable for bulls used in AI. Timed artificial insemination (TAI) represents a breakthrough in the use of AI in Brazil and other countries. Numerous causes contribute to the wide range of results and/or unsatisfactory pregnancy rates in TAI programs, highlighting the factors inherent in the bovine female in addition to several factors inherent to quality of semen used, and in regarding to it, differences reported in fertility could be attributed to variation in sperm qualitative characteristics. Consequently, the success of bovine AI programs largely depends on the use of good quality semen. When only high fertility bulls are used, better conception rates are achieved, reducing costs of reproductive...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Fertility prediction; CASA; Flow cytometer; Predição de fertilidade; Citometria de fluxo; Agricultura Familiar.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1022239
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Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa ArchiMer
Demoy-schneider, Marina; Schmitt, Nelly; Le Pennec, Gael; Suquet, Marc; Cosson, Jacky.
High quality of sperm is essential to a high fertilization rate, especially post- cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure were quantitatively evaluated using images obtained by optical microscopy assisted by the Image J...
Tipo: Text Palavras-chave: Spermatozoa quality; Cryopreservation; Pinctada margaritifera; Motility; Metabolism; CASA.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00453/56421/58114.pdf
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Setting tools for the early assessment of the quality of thawed Pacific oyster (Crassostrea gigas) D-larvae ArchiMer
Suquet, Marc; Le Mercier, Alain; Rimond, Flore; Mingant, Christian; Haffray, Pierrick; Labbe, C..
Parameters used to assess the survival of larvae after cryopreservation generally misestimate the damages that prevent larval development. The objectives of the present study were to 1) define the reliability of the survival rate, assessed at 2 and 7 days post fertilization, to estimate Pacific oyster larval quality after thawing, and 2) select complementary tools allowing an early and reliable estimation of their quality. Oyster larvae were reared for 25 h after fertilization at 19 degrees C and cryopreserved at early D-stage. Then, thawed larvae were incubated in 2-L beakers. At 2 days post fertilization, the survival rate of thawed Pacific oyster larvae was lower than that of fresh larvae for only one experiment (Experiment 3) among the four identical...
Tipo: Text Palavras-chave: ASMA; CASA; Crassostrea gigas; Cryopreservation; D-larvae.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00088/19934/17761.pdf
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Use of diatom motility features as endpoints of metolachlor toxicity ArchiMer
Coquille, Nathalie; Jan, Gwilherm; Moreira, Aurelie; Morin, Soizic.
Many recent ecotoxicological studies suggest a relationship between freshwater contamination and increasing abundances of motile diatoms (potentially able to move). The capacity to escape would present advantages to species in polluted environments. However, actual motility as a response to toxicants had not been described and required experimental validation. We designed a specific experiment to assess how a field-isolated diatom (Gomphonema gracile) distributes energy to in situ resistance (increased population growth or photosynthesis) and escape (behavioral changes), when exposed to increasing concentrations of the herbicide metolachlor. We report here the dose-time dependent responses of G. gracile populations. They coped with low contamination by...
Tipo: Text Palavras-chave: Diatoms; Physiology; Behavior; Pesticide exposure; Motility; CASA.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00254/36521/35081.pdf
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