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A cross-sectional study to estimate the frequency of anti-bovine viral diarrhea virus-1 antibodies in domestic pigs of Mossoró region in the state of Rio Grande do Norte, Brazil Ciência Rural
Gatto,Igor Renan Honorato; Arruda,Andreia Gonçalves; Almeida,Henrique Meiroz de Souza; Silva,Glaucenyra Cecília Pinheiro da; Leite,Alexandro Iris; Samara,Samir Issa; Dutra,Iveraldo dos Santos; Ogata,Renato Akio; Oliveira,Luis Guilherme de.
ABSTRACT: This study investigated the occurrence of antibodies for BVDV-1 in swine herds located in the region of Mossoró city of the state of Rio Grande do Norte, Brazil. A sample size of 412 animals was estimated assuming unknown prevalence (set at 50%). Virus neutralization assay was used to the detect the presence of antibodies for BVDV-1 and the results found were analysed using multivariable logistic regression model. The obtained prevalence was 4% at animal level and 45% at the animal and herd level. The titers were highly variable between animals and within farms. The multivariable logistic regression analysis showed an association between being housed outside and exposure to BVDV-1 (OR=0.24, 95% CI:0.06, 0.96, P=0.04). Highly correlated data and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Swine; Pestiviruses; Virusneutralization; BVDV; CSFV.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901607
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MOLECULAR CLONING AND EXPRESSION OF A FRAGMENT OF THE GENE CODIFYING FOR THE PROTEIN ERNS OF CLASSICAL SWINE FEVER VIRUS Rev Salud Anim.
Agüero,J.A; Sánchez,O; Barrera,Maritza; Toledo,J.R.
Classical swine fever virus (CSFV), belonging to the genus Pestivirus of the Flaviviridae family, is an enveloped positive stranded RNA virus highly contagious that can cause a fatal disease, characterized by fever, leukopenia and hemorrhage, with substantial economic losses. There is a great demand for a marker vaccine against CSFV. C, Erns, E1, and E2 are the structural proteins of the virus. E2 is the best candidate to be incorporated in vaccine, while Erns becomes an ideal candidate as an antigen in a differential diagnostic test. A synthetic fragment of the Erns gene (codifying for aa 109-160) was subcloned into pET28a vector. The cloning was screened by restriction analysis. The gene was expressed as a his-tag fusion protein in BL21 (DE3) E. coli...
Tipo: Journal article Palavras-chave: Erns; CSFV; Recombinant protein; IMAC.
Ano: 2008 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2008000200003
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