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Pharmacological characterization of the relaxant effect induced by adrenomedullin in rat cavernosal smooth muscle BJMBR
Leite,L.N.; Gonzaga,N.A.; Tirapelli,D.P.C.; Tirapelli,L.F.; Tirapelli,C.R..
The aim of the present study was to determine the mechanisms underlying the relaxant effect of adrenomedullin (AM) in rat cavernosal smooth muscle (CSM) and the expression of AM system components in this tissue. Functional assays using standard muscle bath procedures were performed in CSM isolated from male Wistar rats. Protein and mRNA levels of pre-pro-AM, calcitonin receptor-like receptor (CRLR), and Subtypes 1, 2 and 3 of the receptor activity-modifying protein (RAMP) family were assessed by Western immunoblotting and quantitative real-time polymerase chain reaction, respectively. Nitrate and 6-keto-prostaglandin F1α (6-keto-PGF1α; a stable product of prostacyclin) levels were determined using commercially available kits. Protein and mRNA of AM, CRLR,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rat cavernosal smooth muscle; Adrenomedullin; Relaxation; Nitric oxide; Calcitonin receptor-like receptor.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000876
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