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Biagioli,Carolina; Rios,Raúl; Basso,Alicia; Franzone,Pascual; Prina,Alberto. |
Although it is generally accepted that plant in vitro culture response is influenced by the donor genotype, the genetic and molecular bases of this phenomenon are barely known. As a consequence, the optimization of tissue culture protocols is mainly empirically done. Researchers of the IGEAF studied the genetic basis of the in vitro regeneration of various plant species, including the tissue culture response of artificially induced barley mutants. One barley mutant, MC 169, carries a nuclear gene, recently described controlling the root growth in hydroponic cultivation. Under this condition, the roots of MC 169 mutant plants were longer than those of the original wild type line MC 182, a fact that was associated with a reduced ethylene biosynthesis. On the... |
Tipo: Journal article |
Palavras-chave: Callus induction; Ethylene; Habituated calli; Hydroponic culture; Regeneration. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300019 |
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Scotton,Danielle C; Benedito,Vagner Augusto; Molfetta,Jeanne B de; Rodrigues,Benedita Inês FP; Tulmann-Neto,Augusto; Figueira,Antonio. |
Garlic cultivars are sexually sterile under standard growth conditions, with direct implications for commercial production costs as well as breeding programs. Garlic is propagated commercially via bulblets, which facilitates disease transmission and virus load accumulation over vegetative generations. Tissue culture produces virus-free clones that are more productive, while keeping the desired traits of the cultivar. Consequently, this technique allows studies of garlic genetics as well as guarantees genetic conservation of varieties. We aimed at analyzing the in vitro regeneration of eight marketable cultivars of garlic using root segments as explants. For each genotype, bulblet-derived explants were isolated and introduced into MS medium supplemented... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allium sativum; Bulb; Callus induction; Organogenesis; Regeneration; Tissue culture. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000100013 |
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