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Carvalho,Aline Feola de; Silva,Daniela Martins da; Azevedo,Sergio Santos; Piatti,Rosa Maria; Genovez,Margareth Elide; Scarcelli,Eliana. |
Campylobacteriosis is a worldwide distributed zoonosis. One of the main virulence factors related to Campylobacter spp. in animals and humans is the cytolethal distending toxin (CDT), encoded by three adjacent genes (cdtA, cdtB, cdtC). The occurrence of Campylobacter spp. in samples of vegetables has not been reported in Brazil yet, and has seldom been described in the international literature. The detection of CDT in these strains has not been reported, either. The objectives of the present study were to determine the occurrence of Campylobacter spp. strains carrying virulence factors in samples of poultry and vegetables (lettuce and spinach) from different points of sale, thus verifying if vegetables are as an important vehicle for potentially virulent... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter spp.; Cytolethal distending toxin (CDT); Broiler carcasses; Vegetables; Multiplex-PCR. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300005 |
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Benetti,TM; Abrahão,WM; Ferro,ID; Macedo,REF; Oliveira,TCRM. |
ABSTRACT In 2006, the International Organization for Standardization (ISO) published two standard protocols for the detection and enumeration of Campylobacter spp. in foods: the ISO 10272-2(direct method) and the ISO 10272-1 (enrichment method). The aim of this study was to compare the efficiency of these two methods in the detection of Campylobacter spp. and propose a modification in the enrichment method. Compared with the enrichment method, the direct method yielded a higher number of positive results for Campylobacter spp. and, consequently, presented higher sensitivity percentage. The isolation of Campylobacter spp. was more difficult when 10 mL of rinse was used in the enrichment method, which is currently recommended by the ISO protocol. Therefore,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter spp.; Detection; ISO 10272; Refrigerated chicken carcass. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100023 |
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Freitas,J.A.; Noronha,G.N.. |
In order to study Campylobacter in chicken meat exposed to consumption in the city of Belém, state of Pará, Brazil, 16 samples (four of liver, four of gizzard, two of heart, two of skin, one of neck, two of carcass and a mixture of neck skin and fragments of the abdominal cavity), collected in clandestine butcher shops, fair-free and supermarkets were submitted to isolation and identification according to the recommended methods. In high percentage, the foods exposed to consumption were contaminated by this agent, meaning a potential risk to the human health. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carne de frango; Campylobacter spp.; Vigilância sanitária. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300038 |
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