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A simple and reliable PCR-restriction fragment length polymorphism assay to identify Candida albicans and its closely related Candida dubliniensis BJM
Ge,Yi Ping; Wang,Le; Lu,Gui Xia; Shen,Yong Nian; Liu,Wei Da.
Candida dubliniensis is an emerging pathogen capable of causing superficial as well as systemic infections. Due to its close similarity to C. albcians, conventional methods based on phenotypic traits are not always reliable in identification of C. dubliniensis. In this study, we developed a PCR-restriction fragment length polymorphism (RFLP) assay to identify and discriminate between the two closely related species. The D1/D2 region of 28S rDNA was amplified by PCR and enzymatically digested by ApaI and BsiEI respectively. PCR products of both species were digested into two fragments by ApaI, but those of other yeast species were undigested. BsiEI cut the PCR products of C. albicans into two fragments but not those of C. dubliniensis. Thus two species were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Candida dubliniensis; PCR-restriction fragment length polymorphism; Identification; Differentiation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300004
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Actividad antimicrobiana y análisis de la composición química de una fracción de las flores de Acmella ciliata (Kunth) Cass Plantas Medicinales
Rincón Mejía,Carlos Andrés; Castaño Osorio,Jhon Carlos; Gómez Barrera,Milton; Rezek,Jan; Vaněk,Tomáš.
Tipo: Journal article Palavras-chave: Acmella ciliata; Alcamidas; Espilantol; Actividad antimicrobiana; Candida albicans.
Ano: 2015 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962015000400005
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An unusual cause of bilateral scrotal abscess in a preterm infant: Candida albicans BJID
Cetinkaya,Merih; Buyukkale,Gokhan; Payasli,Muge; Ozbek,Sibel; Kavuncuoglu,Sultan.
Acute scrotal abscess is a rare condition in neonates. Most of these abscesses were reported to be unilateral and caused by Staphylococcus and Salmonella spp. Herein, we report a bilateral scrotal abscess in a preterm infant and Candida albicans was isolated from the scrotal fluid culture. To our knowledge, this is the first bilateral scrotal abscess in a preterm infant caused by C. albicans. Therefore, this organism must be suspected in differential diagnosis of acute scrotal abscess in neonates, especially in preterm infants.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Scrotal abscess; Neonate; Preterm infant; Candida albicans.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200023
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Analysis of the in vitro adherence of Streptococcus mutans and Candida albicans BJM
Barbieri,Dicler de Sant'Anna Vitor; Vicente,Vânia Aparecida; Fraiz,Fabian Calixto; Lavoranti,Osmir José; Svidzinski,Terezinha Inês Estivalet; Pinheiro,Rosângela Lameira.
The objective of the present study was to investigate the in vitro adherence capacity of Streptococcus mutans and Candida albicans. Adherence assays were conducted on dental surfaces and analyzed by scanning electron microscopy (SEM). Extracted human teeth were inoculated with Streptococcus mutans or Candida albicans and with both species simultaneously, and incubated at 37ºC for 21 days. Bacterial inocula had been obtained from saliva samples of children that had been colonized by both organisms. ATCC reference strains were used as controls. SEM analyses showed that the biofilm that covered the entire analyzed dental surface was more homogeneous inoculated with the two microorganisms simultaneously than with each species separately. In a second...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adherence; Candida albicans; Streptococcus mutans; Biofilm.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400009
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Antibacterial, anti fungal and E. coli DNA cleavage of Euphorbia prostrata and Pelargonium graveolens extract and their combination with novel nanoparticles BJPS
Dashamiri,Somayeh; Ghaedi,Mehrorang; Naghiha,Reza; Salehi,Amin; Jannesar,Ramin.
As there are a lot of antibacterial and anti-fungal resistant pathogens, researchers attempt to substitute antimicrobial drugs with various medical plants and novel nanoparticles. The present study was conducted to characterize antimicrobial activities of Euphorbia prostrata and Pelargonium graveolens extract alone and in combination with Mn-Ni@Fe3O4-NPs & Mn: Fe (OH)3-NPs on the DNA cleavage of E. coli and also Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Aspergillus oryzae, and Candida albicans. The effects of antimicrobial activities on above scenarios were evaluated using disc diffusion, MIC, MBC, and E. coli DNA electrophoresis methods. The results showed that the effects of antibacterial assay values of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Euphorbia prostrata/extract/antimicrobial activities; Pelargonium graveolens/extract/antimicrobial activities E coli; Candida albicans; Nanoparticles.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400625
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Anticandidal effect of endophytic bacteria isolated from Equisetum arvense L. against Candida albicans and Candida glabrata BABT
Das,Gitishree; Patra,Jayanta Kumar; Choi,Jaehyuk; Baek,Kwang-Hyun.
ABSTRACT Equisetum arvense, a fern species possesses a number of pharmaceutical prospective. In the present study, a total of 103 endophytic bacteria isolated from E. arvense and were evaluated for their anticandidal property against five Candida species, two C. albicans, C. glabrata, C. saitoana and C. geochares. Out of them fifty one were identified as per the morphological and molecular characterisation using 16S rRNA gene sequencing and among them, ten promising endophytic bacteria were mentioned in the present study. Among ten endophytic bacteria, Psychrobacillus insolitus and Curtobacterium oceanosedimentum exerted highest anticandidal effect against C. albicans KACC 30062 and C. glabrata KBNO6P00368, with diameter of inhibition zones of 21.30±0.41...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticandidal effect; Endophytic bacteria; Equisetum arvense; Candida albicans; Candida glabrata.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100455
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Antifungal activity of bacterial strains from the rhizosphere of Stachytarpheta crassifolia. Repositório Alice
SANTOS, S. N.; OLIVEIRA, L. K. X.; MELO, I. S. de; VELOSO, E. da S.; ROQUE, M. R. de A..
This study evaluated the antifungal action of biomolecules produced from the secondary metabolism of bacterial strains found in the rhizosphere of semi arid plants against human pathogenic Candida albicans. Crude extracts were obtained using ethyl acetate as an organic solvent and the bioactivity was assessed with a bioautography technique. The results showed that bacterial strains, Alcaligenes faecalis MRbS12 and Bacillus cereus MRbS26, had compounds with antifungal bioactivity. The largest inhibition zones for both compounds were located on spots with Rf values below 0.500, indicating that the molecules possibly had polar characteristics. These results suggested that microorganisms found in the environment could have bioprospecting potential.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Biomolecules; Bioautoghaphy; Antifungal activity; Alcaligenes faecalis; Bacillus cereus; Candida albicans; Bactéria; Rizosfera; Controle biológico; Rhizobiales; Antifungal agents; Biological control.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/898255
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Antifungal activity of Cymbopogon winterianus jowitt ex bor against Candida albicans BJM
Oliveira,Wylly Araújo de; Pereira,Fillipe de Oliveira; Luna,Giliara Carol Diniz Gomes de; Lima,Igara Oliveira; Wanderley,Paulo Alves; Lima,Rita Baltazar de; Lima,Edeltrudes de Oliveira.
Candida albicans is an opportunistic yeast and a member of the normal human flora that commonly causes infections in patients with any type of deficiency of the immune system. The essential oils have been tested for antimycotic activity and pose much potential as antifungal agents. This work investigated the activity of the essential oil of Cymbopogon winterianus against C. albicans by MIC, MFC and time-kill methods. The essential oil (EO) was obtained by hydrodistillation using a Clevenger-type apparatus. It was tested fifteen strains of C. albicans. The MIC was determined by the microdilution method and the MFC was determined when an aliquot of the broth microdilution was cultivated in SDA medium. The phytochemical analysis of EO showed presence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Antifungal; Cymbopogon winterianus.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200004
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Antifungal activity of some Sternbergia taxa: effects on germ tube and biofilm formation BJPS
Kaskatepe,Banu; Yilmaz,Betul Sever; Acikara,Ozlem Bahadir; Iscan,Gulcin Saltan; Vlainic,Josipa; Kosalec,Ivan.
Natural products are rapidly becoming the primary sources of novel antimicrobial agents, as resistance to existing antimicrobial agents is increasing. Apart from determining the antimicrobial activity of natural products, it is also important to understand their effects on the virulence factors of microorganisms. This study aimed to determine the antimicrobial activity of Sternbergia species prevalent in Turkey and investigate their role in the inhibition of germination tube and biofilm formation, both of which are known to be important virulence factors of Candida albicans. The antimicrobial activities of the plant extracts were evaluated using bore-plate and broth microdilution method. The extracts’ capacity to inhibit the formation of the germ-tube was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal activity; Biofilm inhibition; Candida albicans; Germ tube formation; Virulence factor; Sternbergia spp.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100546
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Antimicrobial activity of meta-alkoxyphenylcarbamates containing substituted N-phenylpiperazine fragment BJM
Malík,Ivan; Bukovský,Marián; Andriamainty,Fils; Gališinová,Jana.
In the present investigation, the basic esters of meta-alkoxyphenylcarbamic acid bearing variously substituted N-phenylpiperazine fragment were screened for their in vitro antimicrobial activity against Staphylococcus aureus, Escherichia coli and Candida albicans, respectively. The most effective against Escherichia coli was found the compound 6d (MIC=195,3 μg/mL) bearing simultaneously para-fluoro substituent at the 4‑phenylpiperazin-1-yl core and meta-methoxy side chain in the lipophilic part of the molecule. From whole analyzed set of the molecules the substance 8e with propoxy side chain forming meta-alkoxyphenylcarbamoyl fragment and lipophilic, sterically bulky meta-trifluoromethyl group attached at N-phenylpiperazine moiety was evaluated as the most...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phenylcarbamates; Substituted N-phenylpiperazines; Candida albicans.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300016
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Antimicrobial, antioxidant and cytotoxicity potential of Manihot multifida (L.) Crantz (Euphorbiaceae) Anais da ABC (AABC)
FABRI,RODRIGO L.; SÁ,DANIEL S. DE; PEREIRA,ANA PATRÍCIA O.; SCIO,ELITA; PIMENTA,DANIEL S.; CHEDIER,LUCIANA M..
Manihot multifida (L.) Crantz (Euphorbiaceae) is widely used in popular medicine for the treatment of infected wounds. This study evaluated thein vitro antioxidant and antimicrobial potential of this species against strains of Gram-positive and Gram-negative bacteria and fungi, known to cause infections in humans. The extracts showed minimal inhibitory concentration (MIC) varying from 39 to 2500 µg/mL for antimicrobial activity. The methanolic extract of fruits, aqueous and hexane extracts of leaves showed a very strong activity against Candida albicans(ATCC 18804) with MIC of 39 µg/mL. Furthermore, the methanolic extract of M. multifida leaves exhibited DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging potential with inhibitory concentration (IC50) values...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial; Antioxidant; Candida albicans; Cytotoxicity; Manihot multifida.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100303
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Antimicrobial effect of para-alkoxyphenylcarbamic acid esters containing substituted N-phenylpiperazine moiety BJM
Malík,Ivan; Bukovský,Marián; Andriamainty,Fils; Gališinová,Jana.
In current research, nine basic esters of para-alkoxyphenylcarbamic acid with incorporated 4-(4fluoro-/3-trifluoromethylphenyl)piperazin-1-yl fragment, 6i-6m and 8f-8i, were screened for their in vitro antimicrobial activity against Candida albicans, Staphylococcus aureus and Escherichia coli, respectively. Taking into account the minimum inhibitory concentration assay (MIC), as the most active against given yeast was evaluated 8i (MIC = 0.20 mg/mL), the most lipophilic structure containing para-butoxy and trifluoromethyl substituents. Investigating the efficiency of the compounds bearing only a single atom of fluorine and appropriate para-alkoxy side chain against Candida albicans, the cut-off effect was observed. From evaluated homological series, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phenylcarbamates; Substituted N-phenylpiperazines; Candida albicans.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200018
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Antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogens Electron. J. Biotechnol.
Loeza-Ángeles,Heber; López-Meza,Joel E; Ochoa-Zarzosa,Alejandra.
Background: The actions of plant antimicrobial peptides (PAP) against intracellular pathogens are poorly known. It has been reported that wheat puroindolines show antibacterial activity against Staphylococcus epidermidis endocyted by macrophages. In this work, we evaluated the intracellular antimicrobial activity of PAP gamma-thionin and thionin Thi2.1 produced by bovine endothelial cells against intracellular Staphylococcus aureus and Candida albicans. We used three host-pathogen models: 1) bovine mammary epithelial cells (BMEC)-S. aureus, 2) bovine endothelial cells (BEC)-S. aureus and 3) BEC-C. albicans, and evaluated the effect of conditioned media from BEC producers of PAP (gamma-thionin and thionin Thi2.1). Results: In the first model, conditioned...
Tipo: Journal article Palavras-chave: Antimicrobial peptides; Arabidopsis thaliana thionin; Capsicum chinense gamma-thionin; Candida albicans; Intracellular pathogens; Staphylococcus aureus.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500001
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Arachidonic acid affects biofilm formation and PGE2 level in Candida albicans and non-albicans species in presence of subinhibitory concentration of fluconazole and terbinafine BJID
Mishra,Nripendra Nath; Ali,Shakir; Shukla,Praveen K..
Candida albicans utilizes arachidonic acid (AA) released during the course of infection (Candidiasis) from phospholipids of infected host cell membranes and synthesizes extracellular prostaglandin(s) which play an important role in hyphae formation and host cell damage. C. albicans biofilms secrete significantly more prostaglandin(s) and evidence suggests that Candida biofilms have dramatically reduced susceptibility to majority of antifungal drugs. AA influences the saturation level of lipids and fluidity of yeast cell membranes. Therefore the aim of this study was to evaluate the effect of AA alone or in combination with antifungal agents on biofilm formation and production of prostaglandin (PGE2) in C. albicans, C. parapsilosis, C. glabrata, C....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachidonic acid; Candida albicans; AmBR; Candida tropicalis; Prostaglandin E2; FDA.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300287
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Atividade antimicrobiana de própolis de Apis mellifera obtidas em três regiões do Brasil Ciência Rural
Fernandes Júnior,Ary; Lopes,Marcelo Milanda Ribeiro; Colombari,Viviani; Monteiro,Aydir Cecília Marinho; Vieira,Eliane Passarelli.
As propriedades biológicas da própolis de Apis mellifera são amplamente relatadas sendo comuns variações nas mesmas em função da região onde foram produzidas. A ação antimicrobiana de própolis obtidas em três regiões do Brasil (Botucatu-SP, Mossoró-RN e Urubici-SC) foi investigada sobre linhagens isoladas de infecções clínicas humanas (Staphylococcus aureus, Escherichia coli, Enterococcus sp, Pseudomonas aeruginosa e Candida albicans). Foram preparados extratos alcoólicos de própolis (EAP) e determinada a Concentração Inibitória Mínima (CIM) seguida do cálculo da CIM90%. A própolis de Botucatu foi a mais eficiente sobre S. aureus (0,3%v/v), Enterococcus sp (1,1%v/v) e C. albicans (2,1% v/v). Para E. coli, a própolis eficiente foi de Urubici (7,0%v/v) e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abelha melífera; Extratos alcoólicos; Bactérias; Candida albicans.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100047
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Biotyping and genotypic diversity among oral Candida albicans strains from caries-free and caries-active healthy children BJM
Mardegan,Rita de Cássia; Klein,Marlise Inêz; Golvea,Magda Baglione; Rodrigues,Janaina Aparecida Oliveira; Gonçalves,Reginaldo Bruno; Höfling,José Francisco.
Candida albicans oral strains collected from caries-free and caries-active healthy children ranging from 24 to 36 months old, were studied. The aim of the study was to determine proteinase and phospholipase activities produced by Candida albicans in the two groups and to determine the phenotypic diversity of these enzymes based on genetic polymorphism using the AP-PCR method. Strains identified by morphological and fermentation tests as C. albicans were grown in proteinase and phospholipase agar media at 37ºC for 7 and 4 days, respectively. After the incubation period, the enzyme activity of the proteinase and phospholipase positive strains was measured. All strains were subjected to AP-PCR, using the arbitrary primer AP-3. The enzymatic analysis showed no...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Genotyping; Proteinase; Phospholipase; Virulence.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100005
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Cancer drugs inhibit morphogenesis in the human fungal pathogen, Candida albicans BJM
Routh,Madhushree M; Chauhan,Nitin M; Karuppayil,S Mohan.
Candida infections are very common in cancer patients and it is a common practice to prescribe antifungal antibiotics along with anticancer drugs. Yeast to hyphal form switching is considered to be important in invasive candidiasis. Targeting morphogenetic switching may be useful against invasive candidiasis. In this study, we report the antimorphogenetic properties of thirty cancer drugs.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Morphogenetic switching; Cancer drugs; Antifungal antibiotics.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300029
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Candida albicans: genotyping methods and clade related phenotypic characteristics BJM
Lyon,Juliana P.; Moraes,Karen C.M.; Moreira,Leonardo M.; Aimbire,Flávio; Resende,Maria Aparecida de.
Several molecular methods, such as Southern blotting hybridization, Multilocus Sequence Typing, and DNA microsatellite analysis, have been employed to genotype Candida albicans. The genotype analysis allows to group strains in clades, that is, a group composed of one ancestor and its descendants. These genotype studies demonstrate that clades distribution is influenced by geographic area as well as that antifungal resistance is associated with particular clades. These findings suggested that C. albicans reproduces mainly in a clonal manner, with certain degree of DNA microevolution. Additionally, virulence factors and site of isolation have also been associated with clade specificity. The present article is a brief review about the methods used for Candida...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Clonal reproduction; Microevolution; Genotyping systems; Phenotypic characteristics.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400003
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Candida albicans morphologies revealed by scanning electron microscopy analysis BJM
Staniszewska,M.; Bondaryk,M.; Swoboda-Kopec,E.; Siennicka,K.; Sygitowicz,G.; Kurzatkowski,W..
Scanning electron microscope (SEM) observations were used to analyze particular morphologies of Candida albicans clinical isolate (strain 82) and mutants defective in hyphae-promoting genes EFG1 (strain HLC52) and/ or CPH1 (strains HLC54 and Can16). Transcription factors Efg1 and Cph1 play role in regulating filamentation and adhesion of C. albicans' morphologies. Comparative analysis of such mutants and clinical isolate showed that Efg1 is required for human serum-induced cell growth and morphological switching. In the study, distinct differences between ultrastructural patterns of clinical strain's and null mutants' morphologies were observed (spherical vs tube-like blastoconidia, or solid and fragile constricted septa vs only the latter observed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Pleomorphism; Ultrastructure; Virulence.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300023
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Candidemia in a brazilian tertiary hospital: microbiological and clinical features over a six-year period J. Venom. Anim. Toxins incl. Trop. Dis.
Mondelli,AL; Niéro-Melo,L; Bagagli,E; Camargo,CH; Bruder-Nascimento,A; Sugizaki,MF; Carneiro,MV; Villas Boas,PJF.
Yeasts are becoming a common cause of nosocomial fungal infections in immunocompromised patients. Such infections often develop into sepsis with high mortality rates. The aim of this study was to evaluate some of the numerous factors associated with the development of candidemia. Medical records were retrospectively analyzed of 98 Candida spp. patients. Results showed that the most prevalent risk factors for developing candidemia were: antibiotics and antifungal agents (93.9% and 79.6%, respectively); the use of central venous catheter (93.9%); mechanical ventilation (73.5%); and parenteral nutrition (60.2%). The main species of Candida found were: C. parapsilosis (37.76%), C. albicans (33.67%); and others (28.57%). C. glabrata showed the highest mortality...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candidemia; Mortality; Associated factors; Candida albicans.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200015
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