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| Bezerra,Vilma Sobral; Campos,Julia Furtado; Silva,Roberto Afonso da; Porto,Tatiana Souza; Lima Filho,José Luiz de; Porto,Ana Lúcia Figueiredo. |
| This study aimed to identify antioxidant peptides from caprine casein hydrolysates by papain application using MALDI-TOF mass spectrometer, and a 2² full factorial design, with 4 axial points, in order to evaluate kinetic parameters (time and pH) effects on the degree of hydrolysis as well as the antioxidant activity of Moxotó goat milk casein peptides. Degree of hydrolysis was determined by total and soluble protein ratio in casein. Antioxidant activity was measured by ABTS method with 2, 2-cation-azinobis (3-ethylbenzothiazoline-6-sulfonic acid). TROLOX was used as standard. Peptide pattern and sequence of antioxidant amino acids were obtained using MALDI-TOF/MS. The highest degree of hydrolysis (28.5%) and antioxidant activity (2329.6 mmol.L TROLOX. mg-... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioactive peptides; Caprine casein; Antioxidant activity. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000300020 |
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