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Registros recuperados: 8
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Antimicrobial resistance in Enterobacteriaceae in Brazil: focus on β-lactams and polymyxins BJM
Sampaio,Jorge Luiz Mello; Gales,Ana Cristina.
ABSTRACT During the last 30 years there has been a dissemination of plasmid-mediated β-lactamases in Enterobacteriaceae in Brazil. Extended spectrum β-lactamases (ESBL) are widely disseminated in the hospital setting and are detected in a lower frequency in the community setting. Cefotaximases are the most frequently detected ESBL type and Klebsiella pneumoniae is the predominant species among ESBL producers. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae became widely disseminated in Brazil during the last decade and KPC production is currently the most frequent resistance mechanism (96.2%) in carbapenem resistant K. pneumoniae. To date KPC-2 is the only variant reported in Brazil. Polymyxin B resistance in KPC-2-producing K. pneumoniae...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ESBL; KPC; NDM; Extended spectrum β-lactamases; New Delhi metallo-β-lactamase; Klebsiella pneumoniae carbapenemase; Polymyxin B; Colistin; Antimicrobial resistance; Carbapenemases; Brazil; Enterobacteriaceae.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500031
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Carbapenem-resistant Acinetobacter baumannii outbreak at university hospital BJM
Takagi,E.H.; Lincopan,N.; Cassettari,V.C.; Passadore,L.F.; Mamizuka,E.M.; Martinez,M.B..
Nineteen clonally related imipenem-resistant Acinetobacter baumannii isolates were recovered from eight intensive care unit patients. All isolates harboured blaOXA-51-like β-lactamase genes and showed the absence of 22 kDa fraction in outer membrane porin profile analysis. It suggests a combination of two mechanisms as responsible for carbapenemresistant phenotypes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acinetobacter; BlaOXA-type genes; Carbapenemases; Nosocomial infection; Brazil.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200024
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Detection and analysis of different interactions between resistance mechanisms and carbapenems in clinical isolates of Klebsiella pneumoniae BJM
Dalmolin,Tanise Vendruscolo; Bianchini,Bianca Vendruscolo; Rossi,Grazielle Guidolin; Ramos,Ana Carolina; Gales,Ana Cristina; Trindade,Priscila de Arruda; Campos,Marli Matiko Anraku de.
Abstract Carbapenems are considered last-line agents for the treatment of serious infections caused by Klebsiella pneumoniae, and this microorganism may exhibit resistance to β-lactam antibiotics due to different mechanisms of resistance. We evaluated 27 isolates of K. pneumoniae resistant to carbapenems recovered from inpatients at the University Hospital of Santa Maria-RS from July 2013 to August 2014. We carried out antimicrobial susceptibility, carbapenemase detection, testing for the presence of efflux pump by broth microdilution and loss of porin by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Genetic similarity was evaluated by ERIC-PCR. High levels of resistance were verified by the minimum inhibitory concentration for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Carbapenemases; Loss of porins; Efflux pump.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300493
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First occurrence of blaOXA-58 in Acinetobacter baumannii isolated from a clinical sample in Southern Brazil BJM
Gusatti,Carolina de Souza; Bertholdo,Lauren Martins; Otton,Letícia Muner; Marchetti,Desirée Padilha; Ferreira,Alessandra Einsfeld; Corção,Gertrudes.
This is the first report of an Acinetobacter baumannii from clinical origin carrying the blaOXA-58 gene in Brazil. The isolate included in this study was from a patient during an outbreak in Porto Alegre, RS, Southern Brazil, in 2007. It was resistant to most of the beta-lactams tested, it has also the blaOXA-65 gene and the ISAba1 sequence located upstream to both blaOXA genes detected and it has a MIC of imipenem of 64 μg/mL.
Tipo: Info:eu-repo/semantics/article Palavras-chave: BlaOXA-58; ISAba1; BlaOXA-65; Carbapenemases; Acinetobacter baumannii.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100027
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Modified Carba NP test for the detection of carbapenemase production in gram-negative rods: optimized handling of multiple samples BJM
Campana,Eloiza H.; Chuster,Stephanie G.; Silva,Isadora R. da; Paschoal,Raphael P.; Bonelli,Raquel R.; Moreira,Beatriz M.; Picão,Renata C..
Abstract The modified Carba NP test presented here may be a valuable tool for laboratories interested in investigating a large number of carbapenemase-producing bacteria in a less-costly way. The test was evaluated against 48 carbapenemase-producing and carbapenemase-non-producing gram-negative bacteria. No false–positive results were obtained, but false-negative results were observed with OXA-23- and GES-carbapenemase-producing isolates. Aeromonas sp. are not testable by Modified Carba NP.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbapenemases; Diagnostics; Gram-negative bacteria.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200242
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Performance of rapid tests for carbapenemase detection among Brazilian Enterobacteriaceae isolates BJM
Pancotto,Lisiane Rech; Nodari,Carolina Silva; Rozales,Franciéli Pedrotti; Soldi,Tatiane; Siqueira,Carolina Gomes; Freitas,Ana Lúcia; Barth,Afonso Luís.
ABSTRACT The global emergence of carbapenemases led to the need of developing new methods for their rapid detection. The aim of this study was to evaluate the performance of the rapid tests for carbapenemase-producing and non-producing Enterobacteriaceae. Carbapenem non-susceptible Enterobacteriaceae from a surveillance study submitted to a multiplex real time PCR for carbapenemase detection were included in this study. The isolates were subjected to the rapid phenotypic tests Carba NP, Blue-Carba and Carbapenem Inactivation Method (CIM). A total of 83 carbapenemase-producing (43) and non-producing (40) isolates were included in the study. The sensitivity/specificity were 62.7%/97.5%, 95.3%/100%, and 74.4%/97.5% for Carba NP, Blue-Carba and CIM,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbapenemases; Rapid tests; Carba NP; Blue-Carba; CIM.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400914
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Risk factors for KPC-producing Klebsiella pneumoniae bacteremia BJID
Tuon,Felipe F.; Rocha,Jaime L.; Toledo,Paula; Arend,Lavinia N.; Dias,Camila H.; Leite,Talita M.; Penteado-Filho,Sergio R.; Pilonetto,Marcelo; Zavascki,Alexandre P..
The molecular epidemiology of carbapenemase-producing Klebsiella pneumoniae (KPC) has been largely investigated, but limited clinical information is available. A case-control study was performed to evaluate the risk factors for KPC bacteremia in hospitalized patients. Cases were patients with KPC bacteremia and controls were patients with non-KPC bacteremia. A total of 85 patients were included, 18 (21.2%) were KPC, and 67 (78.8%) were non-KPC (40 [59.7%] of them were extended-spectrum beta-lactamase producers). All KPC isolates were type 2 producers. These isolates belong to five distinct clones. Multivariate analysis showed that age (odds ratio [OR], 1.06; 95% confidence interval [CI], 1.02 - 1.11; p = 0.004), presence of mechanical ventilation (OR,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Bacteremia risk factors; Klebsiella pneumoniae; Carbapenemases; Carbapenems.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500002
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Unusual association of NDM-1 with KPC-2 and armA among Brazilian Enterobacteriaceae isolates BJMBR
Quiles,M.G.; Rocchetti,T.T.; Fehlberg,L.C.; Kusano,E.J.U.; Chebabo,A.; Pereira,R.M.G.; Gales,A.C.; Pignatari,A.C.C..
We report the microbiological characterization of four New Delhi metallo-β-lactamase-1 (blaNDM-1)-producing Enterobacteriaceae isolated in Rio de Janeiro, Brazil. blaNDM-1 was located on a conjugative plasmid and was associated with Klebsiella pneumoniae carbapenemase-2 (blaKPC-2) or aminoglycoside-resistance methylase (armA), a 16S rRNA methylase not previously reported in Brazil, in two distinct strains of Enterobacter cloacae. Our results suggested that the introduction of blaNDM-1 in Brazil has been accompanied by rapid spread, since our isolates showed no genetic relationship.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Enterobacteriaceae; Carbapenem-resistance; Carbapenemases; Methylase.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200174
Registros recuperados: 8
Primeira ... 1 ... Última
 

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