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Registros recuperados: 12
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Antimicrobial activity of doripenem against Gram-negative pathogens: results from INVITA-A-DORI Brazilian study BJID
Gales,Ana Cristina; Azevedo,Heber D; Cereda,Rosângela Ferraz; Girardello,Raquel; Xavier,Danilo Elias.
In vitro activity of doripenem and comparator antimicrobial agents was evaluated against Gram-negative bacilli recently isolated from Brazilian private hospitals that were enrolled in the INVITA-A-DORI Brazilian Study. A total of 805 unique Gram-negative bacilli were collected from patients hospitalized at 18 medical centers between May/08 and March/09. Each hospital was asked to submit 50 single Gram-negative bacilli isolated from blood, lower respiratory tract or intraabdominal secretions. Bacterial identification was confirmed and antimicrobial susceptibility testing was performed using Clinical Laboratory Standards Institute (CLSI) microdilution method at a central laboratory. CLSI M100-S21 (2011) or US-FDA package insert criteria (tigecycline) was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbapenems; Gram-negative bacteria; Brazil.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600003
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Antimicrobial susceptibility in intensive care units: MYSTIC Program Brazil 2002 BJID
Mendes,Caio; Oplustil,Carmen; Sakagami,Elsa; Turner,Philip; Kiffer,Carlos.
OBJECTIVE: Establish the susceptibility pattern of Gram-negative bacteria causing infections in ICU patients, MYSTIC Program Brazil 2002. MATERIAL AND METHODS: Gram-negative bacteria (n = 503) causing nosocomial infections were collected at seven Brazilian centers. The central laboratory confirmed the identification and performed the susceptibility tests by E-test methodology (AB Biodisk, Solna, Sweden) for meropenem, imipenem, ciprofloxacin, ceftazidime, cefepime, cefotaxime, piperacillin/tazobactam, gentamicin, and tobramycin. Interpretation criteria used were according to National Committee for Clinical Laboratory Standards (NCCLS). RESULTS: Pseudomonas aeruginosa (33%) was the most frequently isolated, followed by A. baumannii (17.1%), K. pneumoniae...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drug resistance; Bacterial; Microbial sensitivity tests; Infection control; Carbapenems.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100008
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Antimicrobial susceptibility of Gram-negative bacteria in Brazilian hospitals: the MYSTIC Program Brazil 2003 BJID
Kiffer,Carlos; Hsiung,Andre; Oplustil,Carmen; Sampaio,Jorge; Sakagami,Elsa; Turner,Philip; Mendes,Caio.
Establish the susceptibility pattern of Gram-negative bacteria causing infections in ICU patients, MYSTIC Program Brazil 2003. Gram-negative bacteria (n = 1,550) causing nosocomial infections were collected at 20 Brazilian centers. The central laboratory confirmed the identification and performed the susceptibility tests by Etest methodology (AB Biodisk, Solna, Sweden) for meropenem, imipenem, ciprofloxacin, ceftazidime, cefepime, cefotaxime, piperacillin/tazobactam, gentamicin, and tobramycin. Interpretation criteria used were according to National Committee for Clinical Laboratory Standards (NCCLS). Pseudomonas aeruginosa (30.3%) was the most frequent isolate, followed by E. coli (18.6%), Klebsiella pneumoniae (16.9%), Acitenobacter baumannii (8.8%), and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drug resistance; Bacterial; Microbial sensitivity tests; Infection control; Carbapenems.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000300004
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Carbapenem stewardship: positive impact on hospital ecology BJID
Lima,Ana Lucia Lei Munhoz; Oliveira,Priscila Rosalba Domingos de; Paula,Adriana Pereira de; Dal-Paz,Karine; Almeida Jr,João Nóbrega de; Félix,Cássia da Silva; Rossi,Flávia.
INTRODUCTION: Excessive group 2 carbapenem use may result in decreased bacterial susceptibility. OBJECTIVE: We evaluated the impact of a carbapenem stewardship program, restricting imipenem and meropenem use. METHODS: Ertapenem was mandated for ESBL-producing Enterobacteriaceae infections in the absence of non-fermenting Gram-negative bacilli (GNB) from April 2006 to March 2008. Group 2 carbapenems were restricted for use against GNB infections susceptible only to carbapenems and suspected GNB infections in unstable patients. Cumulative susceptibility tests were done for nosocomial pathogens before and after restriction using Clinical and Laboratory Standards Institute (CLSI) guide-lines.Vitek System or conventional identification methods were performed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbapenems; Drug resistance; Bacterial ecology.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100001
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Emerging carbapenem-resistant Aeromonas spp. infections in Cali, Colombia BJID
Rosso,Fernando; Cedano,Jorge Andrés; Parra-Lara,Luis Gabriel; Sanz,Ana María; Toala,Alejandra; Velez,Jolian Fernando; Hormaza,María Paula; Moncada,Pablo Andrés; Correa,Adriana.
ABSTRACT Introduction: Aeromonas species are renowned enteric pathogens with virulence determinants linked to human diseases, such as gastroenteritis, skin, soft-tissue and muscle infections, and septicemia. A recent concern of resistance in this organism has emerged, especially the presence carbapenemases. Herein we describe a case series of emerging carbapenem-resistant Aeromonas species infection in our hospital in Cali, Colombia. Materials and methods: Cases from 2012 to 2018 are reported. Clinical data was abstracted from the clinical charts and laboratory information. Phenotypic detection of resistance was identified using the VITEK®2 system (BioMérieux) and broth microdilution MicroScan WalkAway plus System (Beckman Coulter). CARBA NP-test and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Aeromonas hydrophila; Carbapenems; CphA protein; Real-time polymerase chain.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000500336
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Evaluation of phenotypic tests to detect carbapenem-resistant Enterobacteriaceae in colonized patients hospitalized in intensive care units BJID
Perez,Leandro Reus Rodrigues; Rodrigues,Diógenes; Dias,Cícero Gomes.
In this study, we aimed to evaluate the performance of different phenotypic tests to detect carbapenem-resistant Enterobacteriaceae. Three different phenotypic methods were evaluated: (1) combined-disk test of meropenem plus phenylboronic acid or EDTA reading after 24 h and 48 h; (2) selective/chromogenic read after 24 h and after 48 h; and (3) overnight selective enrichment broth containing 10 µg ertapenem disk followed by culture on MacConkey agar. A positive result in at least one of the methods was submitted to PCR for blaNDM-1, blaOXA-48, blaKPC, blaSPM-1, blaIMP, and blaGES detection. Carbapenem-resistant Enterobacteriaceae was detected in 31 (30.4%) of 102 rectal swabs evaluated. All isolates showed to be KPC-2-producing organisms. Results showed...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Carbapenems; Enterobacteriaceae; KPC; Surveillance.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400436
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Increasing prevalence of extended-spectrum-betalactamase among Gram-negative bacilli in Latin America: 2008 update from the Study for Monitoring Antimicrobial Resistance Trends (SMART) BJID
Villegas,Maria Virginia; Blanco,Manuel Guzmán; Sifuentes-Osornio,Jose; Rossi,Flávia.
OBJECTIVES: This analysis of the Study for Monitoring Antimicrobial Resistance Trends (SMART) evaluated the susceptibility patterns of Enterobacteriaceae in Latin America in 2008, with emphasis on susceptibility trends of E. coli and K. pneumoniae. METHODS: Clinical isolates were recovered from intra-abdominal infections (IAI) from 23 centers in 10 Latin American countries. Isolates were sent to a central laboratory for confirmation of identification, antimicrobial susceptibility and ESBL testing, following the Clinical Laboratory Standards Institute (CLSI) guidelines. RESULTS: Of 1,003 Gram-negative bacilli collected from intra-abdominal infections, E. coli and K. pneumoniae were the most commonly isolated organisms, and 26.8% of E. coli and 37.7% of K....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbapenems; Beta-lactamases; Gram-negative bacteria; Drug resistance; ESBL.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100007
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Klebsiella ESBL bacteremia-mortality and risk factors BJID
Tuon,Felipe Francisco; Kruger,Margot; Terreri,Marcos; Penteado-Filho,Sergio R; Gortz,Lucas.
Background: Extended spectrum β-lactamase (ESBL)-producing bacteria have become recognized as a problem in South America. The aim of this study was to evaluate risk factors and mortality rate in bacteremia caused by ESBL-producing Klebsiella pneumoniae in a Brazilian hospital. Methods: A three-year retrospective cohort study with 104 cases of K. pneumoniae bacteremia (61 ESBL and 43 non-ESBL). Several clinical and laboratory variables were evaluated. The outcome of interest was 30-day mortality. The adequate treatment was evaluated according to antibiotic susceptibility. Results: Multivariable analysis showed that central venous catheter and mechanical ventilation were independent risk factors for ESBL. The duration of hospitalization before the bacteremia...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Klebsiella pneumoniae; Beta-lactamases; Bacteremia; Carbapenems.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600016
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Klebsiella pneumoniae with multiple antimicrobial resistance BJID
Mendes,Caio; Kiffer,Carlos; Segura,Adília; Ribeiro,Julival; Turner,Philip.
A Klebsiella pneumoniae strain was isolated from the urine of a patient at one of the centers participating in the 2001 edition of the MYSTIC program in Brazil. The initial phenotypic findings of the isolated K. pneumoniae presented an unusual MIC of 8 mug/mL to meropenem, 2 mug/mL to imipenem, elevated MICs to broad spectrum cephalosporins (ceftazidime/cefotaxime/cefepime MIC > 256 mug/mL), aminoglycosides (gentamycin > 256 mug/mL and tobramycin = 48 mug/mL), piperacillin/tazobactam (MIC > 256 mug/mL) and susceptibility to ciprofloxacin (MIC = 0.25 mug/mL). The strain also tested positive for ESBL production with double-disk and E-test methodologies. More detailed investigation revealed that the strain produced a SHV-4 type enzyme and also lacked...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Klebsiella pneumoniae; Drug effects; Drug resistance; Carbapenems.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100008
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KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil BJM
Biberg,Camila Arguelo; Rodrigues,Ana Claudia Souza; Carmo,Sidiane Ferreira do; Chaves,Claudia Elizabeth Volpe; Gales,Ana Cristina; Chang,Marilene Rodrigues.
The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Carbapenems; Drug-resistant bacteria.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200501
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Prevalence of carbapenem resistant Pseudomonas aeruginosa and Acinetobacter baumannii in high complexity hospital BJID
Baumgart,Ana Milda Karsten; Molinari,Marcelo André; Silveira,Alessandro Conrado de Oliveira.
Pseudomonas aeruginosa and Acinetobacter baumannii are Gram-negative bacilli that in the last decades have become prevalent agents of hospital infection due to high antimicrobial resistance developed by these microorganisms. The present study is a retrospective analysis of all positive cultures for these microorganisms in the period of January 2004 to December 2008. Resistance levels of A. baumannii and P. aeruginosa to carbapenems was high and showed a trend to increase during the period of study. In recent years the increasing incidence and resistance levels of A. baumannii and P. aeruginosa to the antimicrobials used for their treatment in the hospital setting underscores the relevance of infections caused by these bacteria. The selective pressure...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acinetobacter baumannii; Pseudomonas aeruginosa; Drug resistance; Carbapenems; Hospital infection control program.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500002
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Risk factors for KPC-producing Klebsiella pneumoniae bacteremia BJID
Tuon,Felipe F.; Rocha,Jaime L.; Toledo,Paula; Arend,Lavinia N.; Dias,Camila H.; Leite,Talita M.; Penteado-Filho,Sergio R.; Pilonetto,Marcelo; Zavascki,Alexandre P..
The molecular epidemiology of carbapenemase-producing Klebsiella pneumoniae (KPC) has been largely investigated, but limited clinical information is available. A case-control study was performed to evaluate the risk factors for KPC bacteremia in hospitalized patients. Cases were patients with KPC bacteremia and controls were patients with non-KPC bacteremia. A total of 85 patients were included, 18 (21.2%) were KPC, and 67 (78.8%) were non-KPC (40 [59.7%] of them were extended-spectrum beta-lactamase producers). All KPC isolates were type 2 producers. These isolates belong to five distinct clones. Multivariate analysis showed that age (odds ratio [OR], 1.06; 95% confidence interval [CI], 1.02 - 1.11; p = 0.004), presence of mechanical ventilation (OR,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Bacteremia risk factors; Klebsiella pneumoniae; Carbapenemases; Carbapenems.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500002
Registros recuperados: 12
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