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An Attempt to Improve Bovine Mycoplasmatales Primary Isolation. Repositório Alice
LIBERAL, M.H.T.; BOUGHTON, E..
The sensitivity to 30 antibiotics of Mycoplasma (10 strains) and Acholeplasma (8 strains) was tested in an attempt to find a better one for suppression of contamination in the culture media, used for primary isolation of Mycoplasmatales. The most effective antibiotics for suppression of Gram-positive and Gram-negative organisms and did not inhibit Mycoplasmatales were: carbenicillin, vancomycin and fosfomycin. Although none of the antibacterial drugs that are inhibitors of cell wall synthesis was active against Mycoplasmatales, the majority of them were also ineffective against Gram-positive and/or Gram-negative contaminant bacteria.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Mycoplasma; Acholeplasma; Antibiotics; Gram-positive; Gram-negative; Carbenicillin.
Ano: 1994 URL: http://www.alice.cnptia.embrapa.br/handle/doc/105218
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Influence of antibiotics on embryogenic tissue and Agrobacterium tumefaciens suppression in soybean genetic transformation Bragantia
Wiebke,Beatriz; Ferreira,Fabricio; Pasquali,Giancarlo; Bodanese-Zanettini,Maria Helena; Droste,Annette.
The influence of different antibiotic treatments in soybean genetic transformation was evaluated. First, an assay was performed to verify how different antibiotic treatments affect soybean embryogenic tissues. The effect of carbenicillin at 500 mg L-1 was genotype-dependent. This antibiotic did not affect embryo survival of cv. IAS5, but a three-fold increase of embryo proliferation was observed for cv. Bragg, when compared to the control. On the other hand, cefotaxime at 350 and 500 mg L-1 caused death of embryogenic tissues of both cultivars. Finally, the association of cefotaxime (250 mg L-1) + vancomycin (250 mg L-1) did not affect negatively the somatic embryos of tested cultivars until 63 days of treatment. Thereafter, a second experiment was carried...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobacterium tumefaciens; Carbenicillin; Cefotaxime; Genetic transformation; Glycine max; Vancomycin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052006000400002
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