SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Gholizadeh,Ashraf; Kohnehrouz,B. Baghban.

Differential antioxidative activities were assessed in the leaves of two maize inbreds (A-180 and A-619) under salt stress and the subsequent recovery period. Total antioxidation test revealed that in both inbreds, this ability was sharply increased during stress period, but was slowly reverted back to the normal level during recovery. The enzymatic antioxidative analysis showed differential patterns in the activities of catalase, peroxidase and polyphenol oxidase in both maize inbreeds. Comparative analysis of the activity of phenylalanine ammonia lyase (PAL), a key enzyme at the gateway of propanoid biosynthetic pathway, suggested that propanoid compounds might be antioxidants of pivotal importance to the salt-challenged maize antioxidation system. As...

Activity of antioxidative enzymes in watercress and Chinese cabbage plants grown under hydroponic system with brackish water

Palavras-chave: Antioxidation; Maize; Salt stress; PAL; Catalase; Peroxidase; Polyphenol oxidase.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000400001

Horticultura Brasileira

Lira,Raquele M; Silva,Ênio FF; Willadino,Lilia; Oliveira Filho,Ronaldo A; Andrade,Gilvany R.

ABSTRACT Underground water reserves in Semiarid Region present high salinity levels; however, scarcity of resource compels farmers to use this water for conventional agriculture. Biomass production and activity of the antioxidative defense system of Chinese cabbage and watercress were evaluated in plants grown under hydroponic NFT system with brackish water. The experimental design was completely randomized, with four replications and five water salinity levels (1.95; 3.37; 4.48; 6.82 and 7.51 dS m-1), obtained by an increasing addition of NaCl into the local water supply and nutrient salts for preparation of nutrient solution. Both watercress and Chinese cabbage plants decreased in fresh and dry shoot biomass, in relation to increase of salinity in...

Análise da atividade de enzimas oxidativas em raízes de mandioca tolerantes e susceptíveis à deterioração fisiológica pós-colheita.

Palavras-chave: Naturtium officinale; Brassica pekinensis; Superoxide dismutase; Ascorbate peroxidase; Catalase.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000200205

MOTA, J. V. R.; PEREIRA, M. E. C.; VENTURINI, M. T.; OLIVEIRA, E. J. de.

Uma grave restrição à comercialização in natura da mandioca deve-se à deterioração fisiológica pós-colheita (DFPC), em que ocorre o escurecimento dos tecidos vasculares com a participação de enzimas oxidativas.

Tipo: Parte de livro

Palavras-chave: Peroxidase; Catalase; Manihot..

Ano: 2015

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1032194

Antioxidant enzyme expression and its relationship to coffee beans quality.

Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture

FIGUEIREDO, A. G.; ROSA, S. D. V. F. da; CLEMENTE, A. C. S.; MALTA, M. R.; VILELA, A. L..

Tipo: Anais e Proceedings de eventos

Palavras-chave: Post-harvest.; Pós-colheita; Catalase; Enzima; Coffea Arábica..

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1065311

Scientia Agricola

Pompeu,Georgia Bertoni; Gratão,Priscila Lupino; Vitorello,Victor Alexandre; Azevedo,Ricardo Antunes.

Exposure to nickel (Ni) at high concentrations can lead to production of reactive oxygen species (ROS) resulting in oxidative damage at the cellular level. We investigated the antioxidative responses of Nicotiana tabacum cv BY-2 cell suspension to Ni stress (0.075 and 0.75 mM NiCl2) over a 72 h period with special attention to potential alterations in isoenzymes of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR). Two main SOD isoenzymes were observed, a Mn-SOD (band I) and a Fe-SOD (band II), as well as one CAT isoenzyme and four GR isoenzymes. Activity staining analysis revealed that CAT activity plays a major role in the early response to Ni-induced oxidative stress, particularly when the Ni concentration used was low, whilst a...

Anti-oxidative and proteolytic activities and protein profile of laticifer cells of Cryptostegia grandiflora, Plumeria rubra and Euphorbia tirucalli

Palavras-chave: Antioxidant enzymes; Catalase; Nickel; Superoxide dismutase.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500015

Freitas,Cleverson D. T. de; Souza,Diego P. de; Araújo,Eliane S.; Cavalheiro,Mariana G.; Oliveira,Luciana S.; Ramos,Márcio V..

In this study, proteins extracted from laticifer cells of three plants were examined by electrophoresis, mass spectrometry (MALDI-TOF) and characterized in respect of proteolytic, chitinolytic and anti-oxidative activities by means of zymography and colorimetric assays. Acidic proteins with molecular masses between 12.5 and 74.5 kDa predominated in laticifers of P. rubra. This profile was not found in laticifers of C. grandiflora and E. tirucalli. The later was poor in respect of proteins. Strong anti-oxidative activity of superoxide dismutase (E.C. 1.15.1.1) was detected in P. rubra and C. grandiflora latices, and to a lesser extent ascorbate peroxidase (E.C. 1.11.1.1) and isoforms of peroxidase were seen. Catalase (E.C. 1.11.1.6) was detected only in...

Atividade de enzimas antioxidantes em raízes de milho sob toxidez de alumínio.

Palavras-chave: Catalase; Chitinase; Cysteine proteinase; Latex; Peroxidase.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000100002

Atividade do sistema antioxidante e desenvolvimento de aerênquima em raízes de milho "Saracura".

SOUZA, J. B.; ALVES, V. M. C.; SOUZA, I. R. P.; QUEIROZ, C. G. S.; PARENTONI, S. N.; SCHAFFERT, R. E..

Tipo: Parte de livro

Palavras-chave: Glutationa redutase; Ascorbato peroxidase; Estresse oxidativo.; Catalase; Milho; Zea Mays..

Ano: 2002

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/485671

Atividade do sistema antioxidante e desenvolvimento de aerênquima em raízes de milho 'Saracura'

PEREIRA, F. J.; MAGALHAES, P. C.; SOUZA, T. C. de; CASTRO, E. M. de; ALVES, J. D..

Este trabalho teve como objetivo avaliar a influência de sucessivos ciclos de seleção do milho 'Saracura' na atividade das enzimas do sistema antioxidante e a relação dessas enzimas com a capacidade dessa variedade em desenvolver aerênquima. Sementes de 18 ciclos de seleção intercalados do milho 'Saracura' e da cultivar BR 107, sensível à hipoxia, foram semeadas em vasos e em casa de vegetação. As plantas foram submetidas ao alagamento intermitente de dois em dois dias. As amostras de raizes foram coletadas após 60 dias e analisaram-se as atividades das enzimas peroxidase do guaiacol, peroxidase do ascorbato e catalase, além da capacidade das plantas de cada ciclo desenvolverem aerênquima. Ao longo dos ciclos, as plantas apresentaram modificações na...

Tipo: Artigo de periódico

Palavras-chave: Tolerância ao alongamento.; Catalase; Seleção Massal; Zea Mays.; Peroxidases..

Ano: 2010

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/859074

PAB

Pereira,Fabricio José; Magalhães,Paulo César; Souza,Thiago Corrêa de; Castro,Evaristo Mauro de; Alves,José Donizeti.

Este trabalho teve como objetivo avaliar a influência de sucessivos ciclos de seleção do milho 'Saracura' na atividade das enzimas do sistema antioxidante, e a relação dessas enzimas com a capacidade dessa variedade em desenvolver aerênquima. Sementes de 18 ciclos de seleção intercalados do milho 'Saracura' e da cultivar BR 107, sensível à hipoxia, foram semeadas em vasos e em casa de vegetação. As plantas foram submetidas ao alagamento intermitente de dois em dois dias. As amostras de raízes foram coletadas após 60 dias e analisaram-se as atividades das enzimas peroxidase do guaiacol, peroxidase do ascorbato e catalase, além da capacidade das plantas de cada ciclo desenvolverem aerênquima. Ao longo dos ciclos, as plantas apresentaram modificações na...

Avaliação bioquímica da atividade peroxidásica em folhas de bananeira inoculadas com fungo Mycosphaerella fijiensis Morelet.

Palavras-chave: Zea mays; Catalase; Peroxidases; Seleção massal; Tolerância ao alagamento.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000500003

SOUZA, J. L. de; FERNANDES, C. de F.; VIEIRA JUNIOR, J. R.; REIS, N. D.; LIMA, R. F. de; SILVA, J. C. C. da; ANTUNES JÚNIOR, H. A.; SILVA, D. S. G. da; FERNANDES, C. F. C..

O objetivo do trabalho foi avaliar os níveis de atividade da peroxidase em variedades de Musa sp., na presença e ausência do fungo M. fijiensis. Os resultados alcançados até o momento sugerem que a peroxidase pode estar envolvida no mecanismo de defesa da bananeira contra o ataque do fungo M. fijiensis. Esta participação deve envolver ainda enzimas como a catalase e a ascorbato peroxidase, as quais estariam atuando no controle dos níveis de peróxido de hidrogênio.

Tipo: Anais e Proceedings de eventos

Palavras-chave: Pr - proteínas; Ascorbato peroxidase.; Catalase; Mycosphaerella Fijiensis; Musa sp..

Ano: 2011

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/940836

Avaliação da toxicidade do diflubenzuron e P-cloroanilina em indicadores bioquímicos de organismos não-alvo aquáticos.

Bioactivity of Scytonema hofmanni (Cyanobacteria) in Lilium alexandrae in vitro propagation

DANTZGER, D. D.; GOMES, F. B.; JONSSON, C. M.; AOYAMA, H..

Resumo: O uso de produtos agrícolas vem sendo a principal forma de combater parasitas na aquicultura, sendo que o Diflubenzuron, (DFB) é o mais utilizado. Este composto inibe a síntese de quitina, componente do exoesqueleto dos parasitas, e apresenta baixa toxicidade aos peixes. Porém, no ambiente aquático, o DFB pode ser tóxico às espécies não-alvo e, quando degradado, gera p-cloroanilina, (PCA), metabólito potencialmente cancerígeno e mutagênico para o ser humano. Tendo em vista a necessidade de se obter mais informações sobre a toxicidade destes compostos nos organismos aquáticos não-alvo, a proposta deste trabalho foi analisar a atividade enzimática de fosfatases ácida (FAT) e alcalina (Fale), catalase (CAT) e superoxido dismutase (SOD) de microalga...

Tipo: Separatas

Palavras-chave: P-cloroanilina; Indicadores bioquímicos; Fosfatases; Superoxido dismutase; Pseudokirchneriella subcapitata.; Agrotóxico; Inseticida; Toxidez; Microalga; Catalase; Oreochromis Niloticus.; Pesticides; Insecticides; Diflubenzuron; Ecotoxicology; Daphnia similis..

Ano: 2014

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1007601

Electron. J. Biotechnol.

Zaccaro,María Cristina; Kato,Adriana; Zulpa,Gloria; Storni,Mónica Magdalena; Steyerthal,Noemí; Lobasso,Karina; Stella,Ana María.

Cyanobacteria produces bioactive compounds including plant growth regulators. Naphthalene acetic acid (NAA), a toxic substance, is a synthetic plant regulator used in micropropagation. The aim of this work was to evaluate morphogenetic and antioxidant effects produced by intra and extracellular substances from Scytonema hofmanni (Cyanobacteria) during the multiplication in vitro of Lilium alexandrae and to compare them to those produced by NAA. Intra and extracellular cyanobacterial products increased a) bulblets production reaching 83% and 78% of NAA effect, respectively; b) the bulblet diameter compared to NAA; and c) the bulblet survival due to the promotion of antioxidant activity measured as catalase, ascorbate peroxidase, and glutathione reductase...

Tipo: Journal article

Palavras-chave: Ascorbate peroxidase; Bioactive substances; Bulb regeneration; Catalase; Cyanobacteria; Glutathione reductase; Lilium alexandrae; Scytonema hofmanni.

Ano: 2006

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300007

Can Triamcinolone acetonide, platelet-rich plasma, and pentosan polysulfate sodium induce oxidative stress in cultured equine chondrocytes?

Ciência Rural

Palma,Heloisa Einloft; Gallio,Miguel; Silva,Gabriele Biavaschi da; Cantarelli,Camila; Wolkmer,Patrícia; Salazar,Rodrigo Fernando dos Santos; Wergutz,Julien; Krause,Luciana Maria Fontanari; Krause,Alexandre; Brass,Karin Erica; Corte,Flavio Desessards De La.

ABSTRACT: Progressive deterioration and loss of articular cartilage are the final degenerative events common to osteoarthritis (OA). Reactive oxygen species (ROS) play an important role in this chondrocyte catabolic activity, leading to cell death and matrix components breakdown. Intra-articular corticosteroid injections such as triamcinolone acetonide have been used to control pain and inflammation associated with OA. New treatments for OA, platelet-rich plasma and pentosan polysulphate sodium have also been used and further investigations are necessary to determine their safety in joint cells. In this in vitro study, the use of these three substances (triamcinolone acetonide, platelet-rich plasma, and pentosan polysulphate sodium) in healthy...

Catalase activity of different Candida species after exposition to specific antiserum

Palavras-chave: Antioxidant enzymes; Catalase; Glutathione peroxidase; Lipid peroxidation.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700602

BJM

Miyasaka,Natália R.S.; Unterkircher,Carmelinda S.; Shimizu,Mario T..

Antisera were developed in rabbits after challenge with intracellular antigens of Candida albicans, C. tropicalis and C. parapsilosis. Microorganism catalase has been correlated with virulence, resistance to drugs and immunogenicity. The intracellular catalase is consistently present in strains of Candida and in this paper, the enzyme activity was analysed by PAGE after exposition to antisera. The catalases of C. albicans, C. parapsilosis and C. tropicalis were immunogenic and differed in their binding to specific antibodies raised in rabbits. Tests of cross-reactivity between different Candida species showed that when antiserum from C. albicans immunized rabbit was incubated with intracellular extracts of these three Candida species, the catalases...

Changes in the antioxidative enzyme activities and lipid peroxidation in wheat seedlings exposed to cadmium and lead stress

Palavras-chave: Candidiasis; Catalase; Candida; Antigen; Diagnosis.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100009

Dey,Surjendu K.; Dey,Jayashree; Patra,Sanjukta; Pothal,Debasmita.

Wheat seedlings were grown in presence of CdCl2 (0-200 µM) and Pb(NO3)2 (0-2000 µM) separately. The growth of metal-treated seedlings was significantly depressed. The activities of antioxidative enzymes namely superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (POX) were altered both in root and shoot tissues of 7-d-old seedlings. Under Cd stress, SOD activity in roots was undetectable even at the lowest Cd concentration (50 µM) whereas in shoots it declined sharply with increasing Cd levels. The activity of CAT declined to a greater extent in roots than in shoots. Even though the POX activity increased nine times in shoots, a decreasing trend was observed in root tissues due to Cd stress. Under Pb stress, the induction in SOD activity and...

Changes in the TBARs content and superoxide dismutase, catalase and glutathione peroxidase activities in the lymphoid organs and skeletal muscles of adrenodemedullated rats

Palavras-chave: Catalase; Heavy metals; Peroxidase; Superoxide dismutase; Triticum.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000100006

BJMBR

Pereira,B.; Costa-Rosa,L.F.B.P.; Bechara,E.J.H.; Newsholme,P.; Curi,R..

Thiobarbituric acid reactant substances (TBARs) content, and the activities of glucose-6-phosphate dehydrogenase (G6PDh), citrate synthase (CS), Cu/Zn- and Mn-superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPX) were measured in the lymphoid organs (thymus, spleen, and mesenteric lymph nodes (MLN)) and skeletal muscles (gastrocnemius and soleus) of adrenodemedullated (ADM) rats. The results were compared with those obtained for sham-operated rats. TBARs content was reduced by adrenodemedullation in the lymphoid organs (MLN (28%), thymus (40%) and spleen (42%)) and gastrocnemius muscle (67%). G6PDh activity was enhanced in the MLN (69%) and reduced in the spleen (28%) and soleus muscle (75%). CS activity was reduced in all tissues (MLN...

Changes induced by Cu2+ and Cr6+ metal stress in polyamines, auxins, abscisic acid titers and antioxidative enzymes activities of radish seedlings

Palavras-chave: Epinephrine; Lymphoid organs; Superoxide dismutase; Catalase; Glutathione peroxidase; TBARs.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600016

Choudhary,Sikander P.; Bhardwaj,Renu; Gupta,Bishan D.; Dutt,Prabhu; Gupta,Rajinder K.; Kanwar,Mukesh; Dutt,Pitamber.

The present study determined the effects of copper and chromium metals on the endogenous titers of polyamines, auxins, abscisic acid and antioxidative enzyme activities in Raphanus sativus L. cv. Pusa chetki seedlings. Among polyamines, putrescine and spermidine contents were enhanced by Cu2+ metal to 62.44 and 402.8 µg g-1 f.w. respectively over control. Spermine which was not observed in control was recorded in highest concentration (1287.9 µg g-1 f.w.) in Cu2+ metal stressed seedlings. On the other hand Cr6+ metal treated seedlings showed reduced contents of putrescine (1.43 µg g-1 f.w.), cadaverine (0.09 µg g-1 f.w.), spermidine (277.99 µg g-1 f.w.) and spermine (2.29 µg g-1 f.w.) when compared to control and Cu2+ metal treated seedlings. Significant...

Effect of herbicides in the oxidative stress in crop winter species

Palavras-chave: Catalase; Chromium; Copper; Guaiacol peroxidase; Polyamines (PAs); Superoxide dismutase.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000400006

Anais da ABC (AABC)

ALVES,CARLA; COSTA,EDUARDA; SOFIATTI,JESSICA R.; FORTE,CESAR T.; WINTER,FÁBIO L.; HOLZ,CINTHIA M.; KAIZER,ROSILENE R.; GALON,LEANDRO.

ABSTRACT Most herbicides applied in crop field, stay in the soil for a period, affecting next crop or even the plants using as green manure. Nowadays, the use of herbicides grow to increase productivity, mainly in the grain producing region north of Rio Grande do Sul state. The objective of this study was to evaluate the effects of herbicides fomesafen and sulfentrazone on antioxidant system in Avena sativa1, Vicia sativa2, Raphanus sativus and Lupinus albus. The plants were exposed to varying concentrations of fomesafen3 (0, 0.125, 0.25 and 0.5 kg ha -1) and sulfentrazone (0, 0.3, 0.6 and 1.2 kg ha-1). For this, the activities of, ascorbat peroxidase, catalase and guaiacol enzymes were analyzed, and the levels of MDA were quantificated. Fomesafen and...