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Registros recuperados: 29
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A possible role of Drosophila CTCF in mitotic bookmarking and maintaining chromatin domains during the cell cycle Biol. Res.
Shen,Wenlong; Wang,Dong; Ye,Bingyu; Shi,Minglei; Zhang,Yan; Zhao,Zhihu.
BACKGROUND: The CCCTC-binding factor (CTCF) is a highly conserved insulator protein that plays various roles in many cellular processes. CTCF is one of the main architecture proteins in higher eukaryotes, and in combination with other architecture proteins and regulators, also shapes the three-dimensional organization of a genome. Experiments show CTCF partially remains associated with chromatin during mitosis. However, the role of CTCF in the maintenance and propagation of genome architectures throughout the cell cycle remains elusive. RESULTS: We performed a comprehensive bioinformatics analysis on public datasets of Drosophila CTCF (dCTCF). We characterized dCTCF-binding sites according to their occupancy status during the cell cycle, and identified...
Tipo: Journal article Palavras-chave: DCTCF; Cell cycle; Chromatin domains; Mitotic bookmarking; Bioinformatics.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100027
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Alterations in the mitotic index of Allium cepa induced by infusions of Pluchea sagittalis submitted to three different cultivation systems Anais da ABC (AABC)
Rossato,Liana V.; Tedesco,Solange B.; Laughinghouse IV,Haywood D.; Farias,Júlia G.; Nicoloso,Fernando T..
We evaluated the antiproliferative effect of infusions from Pluchea sagittalis using the Allium cepa test. Infusions in three concentrations (2.5, 5, and 25 g dm-3) of leaves cultivated in three environments (in vitro, acclimatized growth chamber, and field) were used. Six onion bulbs were used for each of the eight treatments, and the mitotic index was obtained from 6000 cells per treatment. In conclusion, leaf infusions of P. sagittalis cultivated in the field have a high antiproliferative activity, as well as the cultivation system influences the antiproliferative potential.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antiproliferative effect; Camphorweed; Cell cycle; Medicinal plant; Mutagenicity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400007
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Circadian G2 arrest as related to circadian gating of cell population growth in Euglena OAK
Bolige, Aoen; Hagiwara, Shin-ya; Zhang, Yulan; Goto, Ken; 後藤, 健.
Cell population growth is gated to occur in particular circadian phases, which has been known for over four decades in various organisms including cyanobacteria and human. However, little is known as to which cell cycle phases from G(1) to M are primarily regulated by the circadian rhythm or when in a circadian cycle this primary regulation takes place. We report here that in the flagellate alga Euglena gracilis grown photoautotrophically, the circadian rhythm primarily prevented developmentally matured G(2) cells from progressing to mitosis, such that cell population growth occurred only during subjective night. In addition, we found that the circadian rhythm also arrests G(1)-to-S and S-to-G(2) transitions at particular circadian phases.
Palavras-chave: Cell cycle; Circadian rhythms; Englena gracilis; G2-arrest; Mitosis.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/717
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Clastogenic activity of 2-chlorodeoxyadenosine in mammalian somatic cells Genet. Mol. Biol.
Antonucci,Gilmara Ausech; Takahashi,Catarina Satie.
The base analogue 2-chlorodeoxyadenosine (2-CdA) used for therapy in chronic resistant and advanced lymphoproliferative disorders, is cytotoxic for both dividing and non-dividing lymphocytes. The present work evaluated the clastogenic potential of this drug in vitro in human lymphocytes in culture and in vivo in BALB/c mice bone marrow cells. In human lymphocytes, the clastogenic effect of 2-CdA was studied in G1, S and G2 phases of the cell cycle, using three different concentrations (10, 20 and 40 mug/mL). The endpoints analyzed included mitotic index (MI), proliferation index (PI), sister chromatid exchange (SCE), and chromosomal aberration (CA). Statistical analysis by a variance (ANOVA) test showed a significant increase (p < 0.05) in CA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human lymphocytes; 2-CdA; Chromosomal aberrations; SCE; Cell cycle.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200027
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Cytology, biochemistry and molecular changes during coffee fruit development Braz. J. Plant Physiol.
De Castro,Renato D.; Marraccini,Pierre.
In commercial coffee species (Coffea arabica and Coffea canephora), fruit development is a lengthy process, characterized by tissue changes and evolutions. For example, soon after fecundation and up to mid development, the fruit is mainly constituted of the pericarp and perisperm tissue. Thereafter, the perisperm gradually disappears and is progressively replaced by the endosperm (true seed). Initially present in a "liquid" state, the endosperm hardens as it ripens during the maturation phase, as a result of accumulation of storage proteins, sucrose and complex polysaccharides representing the main reserves of the seed. The last step of maturation is characterized by the dehydration of the endosperm and the color change of the pericarp. Important...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea spp; Bean development; Cell cycle; Endosperm; EST; Gene expression; Pericarp; Perisperm.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100013
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Cytotoxic Effect of Double Emulsion (W/O/W) CuSO4 Loaded PLA Nanoparticles on MKN-45 Gastric Adenocarcinoma Cell Line International Journal of Morphology
Montiel-Eulefi,E; Jara,F; Toro,C; Garcés,M; Leal,P.
Poly (L-lactic acid) (PLA) nanoparticles have the approval of the main institutions for drugs administration and therapeutics. However, the use of lactic acid polymer is controversial because lactic acid has been proposed as an energy source for cancer cells. The aim of this study was to evaluate the cytotoxic, apoptotic and cell cycle properties of PLA and CuSO4-loaded PLA biodegradable nanoparticles on MKN-45 gastric adenocarcinoma cell line. PLA nanoparticles for the delivery of the anticancer active principle CuSO4 were obtained using the double emulsion method. PLA and CuSO4 loaded PLA nanoparticles were morphologically characterized and their size determined using transmission electron microscopy (TEM). The cytotoxicity of this drug delivery system...
Tipo: Journal article Palavras-chave: Copper loaded nanoparticles; Poly-lactic acid; Adenocarcinoma MKN45cells; Cytotoxicity; Cell cycle.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100011
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DNA replication stress: oncogenes in the spotlight Genet. Mol. Biol.
Primo,Luiza M. F.; Teixeira,Leonardo K..
Abstract Precise replication of genetic material is essential to maintain genome stability. DNA replication is a tightly regulated process that ensues faithful copies of DNA molecules to daughter cells during each cell cycle. Perturbation of DNA replication may compromise the transmission of genetic information, leading to DNA damage, mutations, and chromosomal rearrangements. DNA replication stress, also referred to as DNA replicative stress, is defined as the slowing or stalling of replication fork progression during DNA synthesis as a result of different insults. Oncogene activation, one hallmark of cancer, is able to disturb numerous cellular processes, including DNA replication. In fact, extensive work has indicated that oncogene-induced replication...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cancer; Cell cycle; DNA replication; Oncogene; Replication stress.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200301
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Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers ArchiMer
Cavelier, Patricia; Cau, Julien; Morin, Nathalie; Delsert, Claude.
While our knowledge of bivalve gametogenesis has progressed in recent times, more molecular markers are needed in order to develop tissue imaging. Here, we identified stem cell and mitotic markers to further characterize oyster early gametogenesis, mainly through immunofluorescence microscopy. Intense alkaline phosphatase activity, a non-specific marker for stem cells, was detected on the outer edge of the gonad ducts at the post-spawning stage, suggesting an abundance of undifferentiated cells very early during the sexual cycle. This observation was confirmed using an antibody against Sox2, a transcription factor specific for stem or germline cells, which labeled cells in the gonad duct inner mass and ciliated epithelium early during the initial oyster...
Tipo: Text Palavras-chave: Germline cells; Cell cycle; Reproduction; Bivalve; Marine invertebrates.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00409/52100/52809.pdf
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Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells Biol. Res.
Guo,Jian-Ru; Chen,Qian-Qian; Lam,Christopher Wai-Kei; Zhang,Wei.
BACKGROUND: We have investigated the potential anticancer effects of karanjin, a principal furanoflavonol constituent of the Chinese medicine Fordia cauliflora, using cytotoxic assay, cell cycle arrest, and induction of apoptosis in three human cancer cell lines (A549, HepG2 and HL-60 cells). RESULTS: MTT cytotoxic assay showed that karanjin could inhibit the proliferation and viability of all three cancer cells. The induction of cell cycle arrest was observed via a PI (propidium iodide)/RNase Staining Buffer detection kit and analyzed by flow cytometry: karanjin could dose-dependently induce cell cycle arrest at G2/M phase in the three cell lines. Cell apoptosis was assessed by Annexin V-FITC/PI staining: all three cancer cells treated with karanjin...
Tipo: Journal article Palavras-chave: Karanjin; Cell cycle; Apoptosis; Cancer therapy.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100040
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Efficient retrovirus-mediated transfer of cell-cycle control genes to transformed cells BJMBR
Strauss,B.E.; Costanzi-Strauss,E..
The use of gene therapy continues to be a promising, yet elusive, alternative for the treatment of cancer. The origins of cancer must be well understood so that the therapeutic gene can be chosen with the highest chance of successful tumor regression. The gene delivery system must be tailored for optimum transfer of the therapeutic gene to the target tissue. In order to accomplish this, we study models of G1 cell-cycle control in both normal and transformed cells in order to understand the reasons for uncontrolled cellular proliferation. We then use this information to choose the gene to be delivered to the cells. We have chosen to study p16, p21, p53 and pRb gene transfer using the pCL-retrovirus. Described here are some general concepts and specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene therapy; Clinical trial; Retrovirus; Animal model; Cell cycle; Growth arrest.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700016
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Electrical stimulation of saccharomyces cerevisiae cultures BJM
Araújo,Ofelia Q.F.; Coelho,Maria Alice Z.; Margarit,Isabel C.P.; Vaz-Junior,Carlos A.; Rocha-Leão,Maria Helena M..
Modulation of cell endogenous membrane potential by an external electrical field influences the structure and function of membrane compartments, proteins and lipid bi-layer. In this work, the effects of applied potential on Saccharomyces cerevisiae growth were characterized through simple yet conclusive experiments. Cell growth time profile and cell division were investigated as macroscopic response to the electrical stimulation. Control experiments were conducted under identical conditions except for the absence of applied potential. Through comparative analysis, electrical stimulation was verified to alter cell cycle as smaller sized population was observed, suggesting that a synchrony in cell division was promoted. Power spectral analysis was employed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Electrochemical stress; Saccharomyces cerevisiae; Cell cycle; Viability.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100016
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Expression of D-type cyclins in differentiating cells of the mouse spinal cord Genet. Mol. Biol.
Salles,Patricy de Andrade; Fortes,Juliana Chagas; Guedes,Maria Izabel Florindo; Weller,Mathias.
The D-type cyclins form complexes with the cyclin dependent (CD) kinases CDK4 and CDK6 and promote the G1-S phase transition of the cell cycle by antagonizing the retinoblastoma suppresser protein pRB. In the developing nervous system D-type cyclins show spatially and temporally dynamic patterns of expression. We demonstrated that cyclin D1 was transiently expressed in differentiating spinal cord ventral interneurons while cyclin D3 protein was expressed in differentiating motor neurons and dorsal interneurons. This expression of cyclin D3 in neurons of the mantle zone was extended to all regions of the spinal cord at E15.5. The results suggest that cyclin D1 and D3 have specific functions in differentiating neurons. Similarly, in the developing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell cycle; D-type cyclins; Midbrain-hindbrain region; Neurogenesis; Spinal cord.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400032
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Galectin-8 promotes migration and proliferation and prevents apoptosis in U87 glioblastoma cells Biol. Res.
Metz,Claudia; Döger,Remziye; Riquelme,Elizabeth; Cortés,Priscilla; Holmes,Christopher; Shaughnessy,Ronan; Oyanadel,Claudia; Grabowski,Catalina; González,Alfonso; Soza,Andrea.
BACKGROUND: Glioblastoma is one of the most aggressive cancers of the brain. Malignant traits of glioblastoma cells include elevated migration, proliferation and survival capabilities. Galectins are unconventionally secreted glycan-binding proteins that modulate processes of cell adhesion, migration, proliferation and apoptosis by interacting with beta-galactosides of cell surface glycoproteins and the extracellular matrix. Galectin-8 is one of the galectins highly expressed in glioblastoma cells. It has a unique selectivity for terminally sialylated glycans recently found enhanced in these highly malignant cells. A previous study in glioblastoma cell lines reported that Gal-8 coating a plastic surface stimulates two-dimensional motility. Because in other...
Tipo: Journal article Palavras-chave: Galectin-8; Glioblastoma; Cancer; Cell cycle; Apoptosis; Proliferation; Migration.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100033
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Heparan sulfate and cell division BJMBR
Porcionatto,M.A.; Nader,H.B.; Dietrich,C.P..
Heparan sulfate is a component of vertebrate and invertebrate tissues which appears during the cytodifferentiation stage of embryonic development. Its structure varies according to the tissue and species of origin and is modified during neoplastic transformation. Several lines of experimental evidence suggest that heparan sulfate plays a role in cellular recognition, cellular adhesion and growth control. Heparan sulfate can participate in the process of cell division in two distinct ways, either as a positive or negative modulator of cellular proliferation, or as a response to a mitogenic stimulus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heparan sulfate; Glycosaminoglycan; Proteoglycan; Cell division; Cell cycle; Growth factors.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500006
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Heparan sulfate proteoglycans: structure, protein interactions and cell signaling Anais da ABC (AABC)
Dreyfuss,Juliana L.; Regatieri,Caio V.; Jarrouge,Thais R.; Cavalheiro,Renan P.; Sampaio,Lucia O.; Nader,Helena B..
Heparan sulfate proteoglycans are ubiquitously found at the cell surface and extracellular matrix in all the animal species. This review will focus on the structural characteristics of the heparan sulfate proteoglycans related to protein interactions leading to cell signaling. The heparan sulfate chains due to their vast structural diversity are able to bind and interact with a wide variety of proteins, such as growth factors, chemokines, morphogens, extracellular matrix components, enzymes, among others. There is a specificity directing the interactions of heparan sulfates and target proteins, regarding both the fine structure of the polysaccharide chain as well precise protein motifs. Heparan sulfates play a role in cellular signaling either as receptor...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycosaminoglycans and protein interactions; Growth factors; Focal adhesion; Extracellular matrix; Cell cycle; Cell proliferation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300007
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Induction of apoptosis in cancer cells by tumor necrosis factor and butyrolactone, an inhibitor of cyclin-dependent kinases BJMBR
Belizário,J.E.; Sherwood,S.; Beçak,W..
Induction of apoptosis by tumor necrosis factor (TNF) is modulated by changes in the expression and activity of several cell cycle regulatory proteins. We examined the effects of TNF (1-100 ng/ml) and butyrolactone I (100 µM), a specific inhibitor of cyclin-dependent kinases (CDK) with high selectivity for CDK-1 and CDK-2, on three different cancer cell lines: WEHI, L929 and HeLa S3. Both compounds blocked cell growth, but only TNF induced the common events of apoptosis, i.e., chromatin condensation and ladder pattern of DNA fragmentation in these cell lines. The TNF-induced apoptosis events were increased in the presence of butyrolactone. In vitro phosphorylation assays for exogenous histone H1 and endogenous retinoblastoma protein (pRb) in the total cell...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoptosis; Cell cycle; Cyclin-dependent kinases; Cyclin-dependent kinase chemical inhibitors; Tumor necrosis factor.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400016
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Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro Biol. Res.
Gan,Zhihua; Han,Kun; Lin,Shuchen; Hu,Haiyan; Shen,Zan; Min,Daliu.
Abstract Background Ubiquitin specific peptidase 39 (USP39), an essential factor in the assembly of the mature spliceosome complex, has an aberrant expression in several cancer. However, its function and the corresponding mechanism on human osteosarcoma has not been fully explored yet. Methods The mRNA and DNA copies of USP39 were increased in osteosarcoma cancer tissues compared with the one in human normal tissues according to datasets from the publicly available Oncomine database. A further western blot analysis also demonstrated an aberrant endogenous expression of USP39 in three different osteosarcoma cells. Then lentivirus-mediated short hairpin RNA (shRNA) was designed to silence USP39 in human osteosarcoma cell line U2OS, which is used to test...
Tipo: Journal article Palavras-chave: Cell cycle; Migration; Osteosarcoma; Proliferation; USP39.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100210
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Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro Biol. Res.
Pan,Zeya; Pan,Hao; Zhang,Jin; Yang,Yun; Liu,Hui; Yang,Yuan; Huang,Gang; Ni,Junsheng; Huang,Jian; Zhou,Weiping.
BACKGROUND: Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. RESULTS: In the present study, we investigated the functions of USP39 in human hepatocellular carcinoma (HCC) cell line SMMC-7721. We knocked down the expression of USP39 through lentivirus mediated RNA interference. The results of qRT-PCR and western blotting assay showed that both the mRNA and protein levels were suppressed efficiently after USP39 specific shRNA was delivered into SMMC-7721 cells. Cell growth was significantly inhibited as determined by MTT assay. Crystal violet staining indicated that colony numbers and sizes were both reduced after...
Tipo: Journal article Palavras-chave: Ubiquitin Specific Peptidase 39; Lentivirus; Human hepatocellular carcinoma; Cell proliferation; Cell cycle.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100018
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Meta-analysis of the cell cycle related C12orf48 Biocell
Varisli,Lokman.
The cell cycle is a conserved process from yeast to mammals and focuses on mechanisms that regulate the timing and frequency of DNA replication and cell division. The temporal and spatial expression of the genes is tightly regulated to ensure accurate replication and transmission of DNA to daughter cells during the cycle. Although the genes involved in interphase are well studied, most of the genes which are involved in mitotic events still remain unidentified. Since, the discovery of mitosis related genes is still incomplete, we performed a co-expression and gene ontology analysis for revealing novel mitosis regulated genes. In this study, we showed that C12orf48 is co-expressed with well-known mitotic genes. Moreover, it is also co-expressed with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C12orf48; Cell cycle; Co-expression; Gene ontology; Oncomine.
Ano: 2013 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000100002
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microRNA-3129 promotes cell proliferation in gastric cancer cell line SGC7901 via positive regulation of pRb BJMBR
Yang,Shaofeng; Sheng,Nan; Pan,Lili; Cao,Jing; Liu,Jiao; Ma,Ran.
Several microRNAs (miRNAs) have been reported as oncogenes or tumor suppressors in many cancers, including gastric cancer (GC). However, the role and molecular mechanism of miR-3129 in GC is largely unknown. We aimed to explore the function and the underlying molecular mechanism of miR-3129 in GC. Cancer tissues and corresponding adjacent tissues were collected from 50 patients with GC, and the expression of miR-3129 was detected by RT-qPCR. The expression of miR-3129 and pRb in human GC cell line SCG7091 was altered by transient transfection. Thereafter, MTT and flow cytometry assays were used to analyze cell viability and cell cycle. The expression of cyclin E, CDK2, CDK2 inhibitors (p16 and 21), and pRb were detected by RT-qPCR and western blot. A...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MicroRNA-3129; Gastric cancer; PRb; Proliferation; Cell cycle.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600612
Registros recuperados: 29
Primeira ... 12 ... Última
 

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