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Registros recuperados: 26
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A flavoprotein supports cell wall properties in the necrotrophic fungus Alternaria brassicicola ArchiMer
Pigné, Sandrine; Zykwinska, Agata; Janod, Etienne; Cuenot, Stéphane; Kerkoud, Mohammed; Raulo, Roxane; Bataillé-simoneau, Nelly; Marchi, Muriel; Kwasiborski, Anthony; N’guyen, Guillaume; Mabilleau, Guillaume; Simoneau, Philippe; Guillemette, Thomas.
Background: Flavin-dependent monooxygenases are involved in key biological processes as they catalyze a wide variety of chemo-, regio- and enantioselective oxygenation reactions. Flavoprotein monooxygenases are frequently encountered in micro-organisms, most of which require further functional and biocatalytic assessment. Here we investigated the function of the AbMak1 gene, which encodes a group A flavin monooxygenase in the plant pathogenic fungus Alternaria brassicicola, by generating a deficient mutant and examining its phenotype. Results: Functional analysis indicates that the AbMak1 protein is involved in cell wall biogenesis and influences the melanization process. We documented a significant decrease in melanin content in the Äabmak1 strain...
Tipo: Text Palavras-chave: Monooxygenase; Cell wall; Melanin; Fungus; Flavoprotein.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00371/48189/48305.pdf
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Acidic polysaccharides from Psidium cattleianum (Araçá) BABT
Vriesmann,Lúcia Cristina; Petkowicz,Carmen Lúcia de Oliveira; Carneiro,Paulo Irajara Borba; Costa,Maria Eugênia; Beleski-Carneiro,Eliana.
The aim of this work was to study the acidic polysaccharides of Psidium cattleianum. Pectic fractions were obtained by successive extractions with water, EDTA and sodium carbonate (4 and 25ºC). Monosaccharide composition, total carbohydrates, uronic acids and protein contents were determined for each fraction. The yield of water and EDTA-soluble fractions and high content of uronic acid were consistent with the presence of pectins probably arising from the middle lamellae. On the other hand, sodium carbonate-soluble pectins had a higher neutral sugar content, indicating highly branched polysaccharides in these fractions, consistent with pectins from cell wall.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectins; Acidic polysaccharides; Psidium cattleianum; Cell wall.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200001
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Apoplasm hydrostatic pressure on growth of cylindrical cells Braz. J. Plant Physiol.
Pessoa,José Dalton Cruz; Calbo,Adonai Gimenes.
According to the model frequently used as reference, plant cell growth occurs only when turgor surpasses a threshold. This model was proposed considering a cylindrical cell of constant wall thickness immerged in a water solution, with viscoelastic behaviour, hydraulic conductivity, variable extensibility, and unidirectional elongation. The author, Lockhart (1965), did not consider the effects of apoplasm hydrostatic potential, a subject treated later by Calbo and Pessoa (1994) who argued that this component of cell potential would interfere with cell growth rate. To evaluate this effect, where possible the same deductive procedures as those employed by Lockhart were used here, which resulted in a set of equivalent equations for cell growth, turgor and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoplasm; Cell wall; Growth; Lockhart; Model; Plant.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000100003
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Bromatological characteristics and in vitro digestibility of four sugarcane varieties subjected or not to the application of quicklime - doi: 10.4025/actascianimsci.v34i4.13775 Animal Sciences
Oliveira, Mauro Dal Secco; Universidade Estadual Paulista “Júlio de Mesquita Filho”; Rêgo, Aníbal Coutinho do; Universidade Estadual Paulista “Júlio de Mesquita Filho”; Sforcini, Mariana Paula Rossi; Universidade Estadual Paulista “Júlio de Mesquita Filho”; Freitas Junior, José Esler; Universidade Estadual Paulista “Júlio de Mesquita Filho”; Santos, Juliana dos; Instituto Federal do Rio Grande do Sul; Carvalho, Marina Vieira de.
The experiment was conducted to evaluate the bromatological characteristics and the in vitro digestibility of four sugarcane varieties, subjected or not to hydrolysis, with quicklime. A completely randomized design was employed with three replications arranged in a 4 × 2 factorial scheme, with four sugarcane varieties (SP 52454, RB 867515, RB 855536 and IAC 862480), hydrolyzed or not. There was significant effect on brix (p < 0.05) and industrial fiber (p < 0.05), and IAC 862480 variety had the lowest levels of industrial fiber. There were no significant difference (p > 0.05) in neutral detergent fiber, acid detergent fiber and lignin levels among the sugarcane varieties under analysis and for the sugarcanes, hydrolyzed or not. The use...
Tipo: Análise laboratorial Palavras-chave: 5.04.03.02-8 calcium oxide; Cell wall; Fiber; Ruminants; Saccharum officinarum L..
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/13775
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Cloning and endogenous expression of a Eucalyptus grandis UDP-glucose dehydrogenase cDNA Genet. Mol. Biol.
Labate,Mônica T. Veneziano; Bertolo,Ana L. Ferreira; Nascimento,Daniela Defávari do; Gutmanis,Gunta; Andrade,Alexander de; Rodrigues,Maria J. Calderan; Camargo,Eduardo L.O.; Boaretto,Luis Felipe; Moon,David H.; Bragatto,Juliano; Labate,Carlos A..
UDP-glucose dehydrogenase (UGDH) catalyzes the oxidation of UDP-glucose (UDP-Glc) to UDP-glucuronate (UDP-GlcA), a key sugar nucleotide involved in the biosynthesis of plant cell wall polysaccharides. A full-length cDNA fragment coding for UGDH was cloned from the cambial region of 6-month-old E. grandis saplings by RT-PCR. The 1443-bp-ORF encodes a protein of 480 amino acids with a predicted molecular weight of 53 kDa. The recombinant protein expressed in Escherichia coli catalyzed the conversion of UDP-Glc to UDP-GlcA, confirming that the cloned cDNA encodes UGDH. The deduced amino acid sequence of the cDNA showed a high degree of identity with UGDH from several plant species. The Southern blot assay indicated that more than one copy of UGDH is present...
Tipo: Info:eu-repo/semantics/article Palavras-chave: UDP-glucose; UDP-glucuronate; Hemicellulose; Pectin; Cell wall.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400015
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Coffee carbohydrates Braz. J. Plant Physiol.
Redgwell,Robert; Fischer,Monica.
This review summarises recent advances in the chemistry, physiology and molecular properties of coffee carbohydrates with a particular focus on the cell wall polysaccharides. The results of detailed chemical studies have demonstrated novel structural features of both the galactomannans and the arabinogalactan polysaccharides of the green and roasted coffee bean. For the first time immunological probes based on monoclonal antibodies for specific polysaccharide epitopes were used to reveal the patterns of distribution of the galactomannans, arabinogalactans and pectic polysaccharides in the coffee bean cell wall. Finally, the results of physiological and molecular studies are presented which emphasise the growing awareness of the potential role the metabolic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; Cell wall; Beverage quality; Seed.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100012
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Differences in the cell wall architecture of melanin lacking and melanin producing Cryptococcus neoformans clinical isolates from India: an electron microscopic study BJM
Mandal,Piyali; Roy,Tara S.; Das,Taposh K.; Banerjee,Uma; Xess,Immaculata; Nosanchuk,Joshua D..
Cryptococcus neoformans is an important opportunistic fungal pathogen that causes life-threatening infection of the central nervous system. A major virulence factor for C. neoformans is the production of melanin in the cell wall. Using transmission electron microscopy, we studied the cell walls of three pairs of isolates obtained from patients with dual cryptococcal infections, where a melanotic and an albino strain were isolated from the CSF of each patient. Transmission Electron Microscopy revealed that the albino strains lacked a melanin layer whereas a melanin layer was associated with the cell wall of the melanotic strains, comprising approximately 75% of the cell wall area. The cell wall size of the melanin producing cells was approximately double...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryptococcus neoformans; Melanin; TEM; Cell wall.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400015
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Ethylene-dependent regulation of an alpha-L-arabinofuranosidase is associated to firmness loss in 'Gala' apples under long term cold storage. Repositório Alice
STORCH, T. T.; FINATTO, T.; PEGORARO, C.; DAL CERO, P.; LAURENS, F.; ROMBALDI, C. V.; QUECINI, V.; GIRARDI, C. L..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Methylcyclopropene; A-L-Arabinofuranosidase; Cell wall; Gene expression; Malus x domestica.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1012162
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Evaluation of Digestibility of Cooked Rice Grain using in vitro Digestion Technique CIGR Journal
Ogawa, Y..
Impact of postharvest and/or food processing (such as flour milling to agricultural and food-resource materials) on the digestibility of foodstuffs was investigated through a simulated gastro-intestinal in vitro digestion technique. The differences among the starch digestibilities of cooked rice grain and slurry were examined. In vitro digestion techniques are commonly applied to starch-based milled materials such as flours used for bread making. However, rice is not usually milled to flour but cooked as a whole for consumption. Therefore, maintaining an intact structure of cooked grain during in vitro digestion is very important to evaluate its starch digestibility. Two hundred grams of polished rice grain were cooked using electric rice cooker with 300...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Grain; Slurry; Tissue structure; Cell wall; Starch; In vitro digestion; Japan.
Ano: 2015 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/3139
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Fertilización foliar con boro en aguacatero (Persea americana Mill.). Colegio de Postgraduados
González Gervacio, Catalina.
El boro es un micronutrimento que posee un intervalo muy estrecho entre deficiencia y toxicidad, varias de sus funciones dentro las plantas aún no han sido bien identificadas; la función principal a la que se atribuye el boro es estructural en la pared y membrana celular al formar parte del complejo B-RG II. Sin embargo, tiene varias funciones más como es el transporte de azúcares, síntesis de aminoácidos, proteínas y fenoles. La presente investigación se realizó con los objetivos de determinar la ruta de penetración del boro a través de la hoja y conocer los efectos que aplicaciones foliares de este elemento tienen sobre el contenido de boro total, concentraciones de carbohidratos y aminoácidos en hoja y sobre el grosor de la pared celular en brotes de...
Palavras-chave: Persea americana Mill.; Boro; Rutas de penetración; Carbohidratos; Aminoácidos; Pared celular; Boron; Routes of penetration; Carbohydrates; Amino acids; Cell wall; Maestría; Edafología.
Ano: 2011 URL: http://hdl.handle.net/10521/449
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Fertilización foliar con boro en aguacatero (Persea americana Mill.). Colegio de Postgraduados
González Gervacio, Catalina.
El boro es un micronutrimento que posee un intervalo muy estrecho entre deficiencia y toxicidad, varias de sus funciones dentro las plantas aún no han sido bien identificadas; la función principal a la que se atribuye el boro es estructural en la pared y membrana celular al formar parte del complejo B-RG II. Sin embargo, tiene varias funciones más como es el transporte de azúcares, síntesis de aminoácidos, proteínas y fenoles. La presente investigación se realizó con los objetivos de determinar la ruta de penetración del boro a través de la hoja y conocer los efectos que aplicaciones foliares de este elemento tienen sobre el contenido de boro total, concentraciones de carbohidratos y aminoácidos en hoja y sobre el grosor de la pared celular en brotes de...
Palavras-chave: Persea americana Mill.; Boro; Rutas de penetración; Carbohidratos; Aminoácidos; Pared celular; Boron; Routes of penetration; Carbohydrates; Amino acids; Cell wall; Maestría; Edafología.
Ano: 2011 URL: http://hdl.handle.net/10521/449
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Genome walking in Sugarcane: an approach to clone unknown flanking sequences. Repositório Alice
LEAO, A. P.; SOUTO, B. de M.; DIAS, B. B. A.; MOLINARI, H. B. C..
2011
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sugarcane; Genome walking; Promoter; Genes; Cell wall.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/908733
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Hypoglycemic activity of polysaccharide fractions containing ß-glucans from extracts of Rhynchelytrum repens (Willd.) C.E. Hubb., Poaceae BJMBR
De Paula,A.C.C.F.F.; Sousa,R.V.; Figueiredo-Ribeiro,R.C.L.; Buckeridge,M.S..
ß-Glucans are soluble fibers with physiological functions, such as interference with absorption of sugars and reduction of serum lipid levels. The objective of the present study was to analyze the distribution of ß-glucans in different tissues of the African grass species Rhynchelytrum repens and also to evaluate their hypoglycemic activity. Leaf blades, sheaths, stems, and young leaves of R. repens were submitted to extraction with 4 M KOH. Analysis of the fractions revealed the presence of arabinose, glucose, xylose, and traces of rhamnose and galactose. The presence of ß-glucan in these fractions was confirmed by hydrolyzing the polymers with endo-ß-glucanase from Bacillus subtilis, followed by HPLC analysis of the characteristic oligosaccharides...
Tipo: Info:eu-repo/semantics/article Palavras-chave: SS-Glucans; Cell wall; Hypoglycemic activity; Diabetes mellitus; Grasses; Rhynchelytrum repens.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600010
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Immunocytochemistry of the mucilage cells of Araucaria angustifolia (Bertol.) Kuntze (Araucariaceae) Rev. Bras. Bot.
Mastroberti,Alexandra Antunes; Mariath,Jorge Ernesto de Araujo.
The use of monoclonal antibodies for specific pectic epitopes is an important tool in the study of the cell wall. Throughout the development of mucilage cells of Araucaria angustifolia (Bertol.) Kuntze, a gradient of distribution was observed in relation to the pectic de-esterification, as well as to the increase of galactan and arabinan epitope distribution, and to the reduction of arabinogalactans proteins (AGPs) epitope at maturity. AGP and methyl-esterified homogalacturonan (HGA) were present in the mucilage. Galactans and arabinans were also observed in the mucilage, though with weak labelling. Degradation of AGP in the maturity of mucilage cells, in cell wall, as well as in the secretion, could be involved in the programmed cell death (PCD)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Araucaria angustifolia; Cell development; Cell wall; Monoclonal antibodies; Mucilage cells; Pectin.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042008000100002
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Immunomodulatory properties of cell wall extract from Enterococcus faecalis CECT7121 BJID
Sparo,Mónica; Delpech,Gastón; Batisttelli,Susana; Basualdo,Juan Ángel.
The aim of this study was to investigate the immunomodulatory properties of cell wall extract from Enterococcus faecalis CECT7121, measuring the induction of cytokines TNF-α, IL-6, IL-10 and IL-12 from human peripheral blood mononuclear cells (PBMCs). Cell wall extract was prepared from their growth in brain heart infusion broth (18 h, 35 °C). Subsequently, toxicity of the obtained cell wall extract was tested in Balb-C mice. PBMCs were isolated from buffy coats at the Blood Transfusion Service of Hospital Ramón Santamarina (Tandil, Argentina). PBMCs were purified using standard Ficoll-Paque gradient centrifugation. Aliquots of purified leukocytes were incubated at 37 °C for 24 h with heat-killed E. faecalis CECT7121 and cell wall extract. Concentrations...
Tipo: Info:eu-repo/semantics/report Palavras-chave: E. faecalis CECT7121; Cell wall; Immunomodulationa.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000500551
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Involvement of peroxidases in structural changes of barley stem Bragantia
Lidija,Begović; Lepeduš,Hrvoje; Lalić,Alojzije; Štolfa,Ivna; Jurković,Zorica; Kovačević,Josip; Cesar,Vera.
ABSTRACT The aim of this study was to investigate anatomical characteristics of barley (Hordeum vulgare L.) internodes and to reveal the connection with activity of guaiacol-peroxidases, their isoenzyme profiles, lignin, and cellulose content. Three lower internodes were sampled at elongation stage and differed in their anatomical features by showing that lignin deposition follows developmental pattern. Epidermis and hypodermis in the first internode were characterized by continuous layer of the lignified cells while in the second and third internode thin walled parenchyma cells could be observed in the hypodermis. These observations were in accordance with increment of peroxidase activity from the first to the third internode. Two acidic and 2 basic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lignin; Cell wall; Internode; Hordeum vulgare L..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000300352
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Mecanismos de tolerância da variedade de milho "Saracura" (BRS 4154) ao alagamento. Repositório Alice
ALVES, J.D.; MAGLHAES, M.M.; GOULART, P. de F.P.; DANTAS, B.F.; GOUVEA, J.A. de; PURCINO, R.P.; MAGALHAES, P. C.; FRIES, D.D.; LIVRAMENTO, D.E. do; MEYER, L.E.; SEIFFERT, M.; SILVEIRA, T..
Esta revisao tem por objetivo discutir mecanismos de tolerancia desenvolvidos por plantula da variedade de milho "Saracura" (BRS 4154), que possui, como principal caracteristica, tolerancia a periodos intermitentes de encharcamento do solo. Os primeiros estudos de analise de tolerancia a baixa disponibilidade de oxigenio no meio, sob condicoes controladas, em sala de crescimento, confirmaram essa caracteristica, ao revelar que as plantulas suportam ate quatro dias de hipoxia sem nenhum dano aparente. A partir dai, apresentaram na regiao do mesocotilo, uma regiao translucida, caracteristica de lise celular, a qual evoluiu para uma constricao, como o prolongamento do estresse, causando murcha, tombamento e morte das plantulas. Estudos posteriores revelaram...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Milho; Hipoxia; Calcio; Parede celular; Enzimas; Tolerancia; Alagamento; Zea mays; Maize; Calcium; Cell wall; Enzymes; Flooding.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/480572
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Mecanismos de tolerancia da variedade de milho Saracura (BRS 4154) ao alagamento. Repositório Alice
ALVES, J. D.; MAGALHAES, M. M.; GOULART, P. de F. P.; DANTAS, B. F.; GOUVEA, J. A. de; PURCINO, R. P.; MAGALHAES, P. C.; FRIES, D. D.; LIVRAMENTO, D. E. do; MEYER, L. E.; SEIFFERT, M.; SILVEIRA, T..
bitstream/item/187099/1/bARBARA-2002.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Hipoxia; Parede celular; Maize; Variety; Cell wall; Cálcio; Enzima; Milho; Variedade; Calcium; Enzymes.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/134585
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Molecular characterization and functional analysis of the beta-galactosidase gene during Coffea arabica (L.) fruit development. Repositório Alice
FIGUEIREDO, S. A.; LASHERMES, P.; ARAGAO, F. J. L..
bitstream/item/183324/1/erq440.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cell wall; Fruit ripening; Coffee; Café; Coffea Arábica; Beta-galactosidase.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/901379
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Occurrence of xyloglucan containing protuberances in the storage cell walls of cotyledons of Hymenaea courbaril L. Rev. Bras. Bot.
TINÉ,MARCO AURÉLIO SILVA; CORTELAZZO,ANGELO; BUCKERIDGE,MARCOS S..
Despite the suggestions of its pectic composition, no clear evidence for this has been presented. Here we show the occurrence of such a structure in walls of cells from cotyledons of Hymenaea courbariI L. These cells are known to accumulate large amounts of storage xyloglucan in the wall and, in this case, the protuberances seem to contain this storage polysaccharide rather than pectin. A hypothetical sequence of events leading from wall strands to protuberances was assembled based on scanning electron microscopy observations. On this basis, a tentative model for how polysaccharides are distributed into the wall, near the regions where protuberances are found, is proposed to explain the presence of storage xyloglucan in their composition.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xyloglucan; Pectin; Cell wall; Protuberances; Hymenaea courbaril.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042000000400007
Registros recuperados: 26
Primeira ... 12 ... Última
 

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